GREMLIN Database
LEU1 - Probable 2-isopropylmalate synthase
UniProt: Q97W36 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 347 (336)
Sequences: 4949 (2436)
Seq/√Len: 132.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
54_D96_Y3.6251.00
265_H269_E3.5141.00
149_Y186_A3.2411.00
19_P44_G3.2001.00
72_E83_K3.0791.00
167_V183_G2.7351.00
82_T85_E2.6861.00
98_K132_D2.6831.00
88_N92_E2.6711.00
90_I122_A2.6531.00
156_V165_V2.6291.00
146_F185_Y2.6101.00
7_A40_V2.6011.00
123_I134_I2.5951.00
153_K157_N2.5181.00
7_A39_Q2.4871.00
116_E154_K2.4711.00
72_E115_E2.3501.00
169_C180_S2.3301.00
77_Y270_N2.2921.00
184_V191_A2.2451.00
50_K92_E2.2391.00
81_M89_K2.2351.00
278_S281_E2.1841.00
84_E121_T2.1841.00
91_Y129_A2.1771.00
9_L232_V2.1721.00
91_Y95_K2.1441.00
304_E309_Y2.1171.00
196_N233_S2.0781.00
174_G209_E2.0741.00
8_D228_K2.0701.00
90_I126_A2.0321.00
70_T114_T1.9711.00
5_K8_D1.9681.00
228_K231_E1.9521.00
23_E27_I1.9521.00
31_K35_E1.9281.00
154_K158_F1.9211.00
75_M83_K1.9161.00
211_V224_V1.8891.00
75_M86_V1.8721.00
149_Y185_Y1.8521.00
311_S314_K1.8481.00
136_I155_I1.8401.00
50_K96_Y1.8181.00
50_K54_D1.8141.00
91_Y125_T1.8131.00
53_I93_S1.7691.00
78_K109_E1.7671.00
30_R34_E1.7641.00
233_S244_V1.7601.00
5_K235_F1.7071.00
180_S191_A1.7001.00
164_I189_R1.6981.00
136_I151_M1.6921.00
120_F154_K1.6841.00
225_K228_K1.6611.00
125_T128_E1.6611.00
209_E248_K1.6391.00
4_R35_E1.6161.00
27_I31_K1.6161.00
321_E324_K1.5891.00
299_L318_V1.5851.00
149_Y153_K1.5811.00
324_K328_S1.5771.00
153_K186_A1.5451.00
124_R160_G1.5411.00
300_R304_E1.5271.00
84_E88_N1.5181.00
70_T122_A1.5131.00
330_N336_D1.5091.00
87_L122_A1.5061.00
165_V188_A1.4991.00
83_K118_F1.4931.00
81_M85_E1.4901.00
106_F122_A1.4881.00
275_E286_R1.4841.00
148_Y167_V1.4831.00
31_K34_E1.4801.00
205_A210_V1.4781.00
230_Y284_N1.4761.00
301_K304_E1.4741.00
91_Y128_E1.4741.00
124_R158_F1.4651.00
143_M148_Y1.4641.00
300_R312_D1.4411.00
54_D100_H1.4391.00
303_L341_A1.4151.00
83_K115_E1.4091.00
94_V104_V1.4001.00
54_D58_S1.3891.00
113_R147_K1.3801.00
267_V287_R1.3771.00
87_L121_T1.3751.00
259_E291_G1.3741.00
152_I165_V1.3731.00
211_V226_T1.3701.00
27_I30_R1.3691.00
44_G56_T1.3631.00
325_L332_V1.3621.00
72_E76_K1.3611.00
41_E103_I1.3611.00
302_L305_E1.3531.00
108_P123_I1.3451.00
319_L323_K1.3451.00
318_V340_I1.3431.00
195_V236_V1.3361.00
296_I316_K1.3351.00
320_A324_K1.3181.00
150_D154_K1.3061.00
19_P29_T1.3021.00
72_E75_M1.2941.00
96_Y100_H1.2901.00
94_V129_A1.2821.00
120_F155_I1.2741.00
235_F239_M1.2711.00
83_K121_T1.2621.00
5_K232_V1.2591.00
152_I186_A1.2571.00
212_M226_T1.2551.00
85_E88_N1.2501.00
13_V103_I1.2441.00
103_I132_D1.2371.00
24_G327_E1.2351.00
57_I100_H1.2341.00
3_A28_A1.2321.00
181_L213_G1.2311.00
156_V189_R1.2311.00
60_G63_S1.2241.00
75_M114_T1.2221.00
259_E293_H1.2121.00
193_V207_L1.2061.00
75_M115_E1.2041.00
117_D121_T1.2041.00
117_D120_F1.2031.00
123_I136_I1.1880.99
171_N174_G1.1840.99
26_F55_K1.1770.99
127_I160_G1.1760.99
91_Y126_A1.1750.99
152_I155_I1.1750.99
112_T143_M1.1740.99
152_I167_V1.1740.99
127_I163_I1.1690.99
138_D179_N1.1660.99
230_Y234_R1.1540.99
67_F262_I1.1530.99
120_F124_R1.1430.99
177_T210_V1.1370.99
81_M86_V1.1320.99
75_M118_F1.1280.99
32_I42_V1.1130.99
303_L310_L1.1120.99
209_E249_A1.0970.99
125_T129_A1.0950.99
109_E274_Y1.0940.99
120_F158_F1.0930.99
180_S210_V1.0900.99
116_E151_M1.0880.99
180_S205_A1.0850.99
215_K221_E1.0790.99
182_A186_A1.0720.99
12_D223_N1.0620.99
87_L125_T1.0580.99
55_K58_S1.0560.99
5_K228_K1.0340.98
193_V211_V1.0270.98
84_E87_L1.0240.98
20_A51_N1.0210.98
110_D113_R1.0200.98
301_K305_E1.0180.98
124_R128_E1.0170.98
47_R52_D1.0160.98
95_K129_A1.0150.98
127_I134_I1.0130.98
68_I106_F1.0060.98
46_S56_T0.9990.98
317_I320_A0.9980.98
171_N249_A0.9860.98
316_K320_A0.9860.98
80_K269_E0.9830.98
61_I102_M0.9790.98
97_A104_V0.9780.98
215_K281_E0.9720.98
92_E96_Y0.9690.98
174_G206_S0.9670.98
12_D39_Q0.9620.98
182_A185_Y0.9550.97
113_R142_T0.9550.97
112_T151_M0.9540.97
171_N176_A0.9520.97
124_R159_V0.9480.97
300_R315_L0.9480.97
302_L334_V0.9470.97
90_I125_T0.9440.97
78_K265_H0.9380.97
146_F150_D0.9340.97
37_G60_G0.9330.97
256_F282_V0.9250.97
11_V224_V0.9250.97
48_S93_S0.9230.97
154_K157_N0.9180.97
29_T44_G0.9130.97
174_G249_A0.9090.97
310_L318_V0.9090.97
107_S137_P0.9060.97
69_A74_H0.9040.96
73_L77_Y0.9040.96
5_K231_E0.8980.96
198_I242_V0.8980.96
74_H110_D0.8940.96
69_A110_D0.8930.96
175_L181_L0.8930.96
177_T205_A0.8840.96
4_R37_G0.8780.96
72_E114_T0.8720.96
148_Y169_C0.8640.95
146_F149_Y0.8620.95
78_K274_Y0.8610.95
69_A109_E0.8600.95
78_K273_T0.8600.95
299_L315_L0.8580.95
75_M81_M0.8570.95
117_D154_K0.8550.95
24_G292_K0.8550.95
79_L109_E0.8540.95
312_D315_L0.8520.95
103_I133_R0.8510.95
246_Y253_D0.8480.95
16_A29_T0.8450.95
63_S103_I0.8440.95
26_F58_S0.8430.95
79_L274_Y0.8420.95
122_A126_A0.8400.95
105_E135_N0.8350.95
150_D153_K0.8310.94
121_T125_T0.8300.94
299_L319_L0.8250.94
180_S193_V0.8240.94
43_I63_S0.8200.94
123_I155_I0.8190.94
41_E62_S0.8170.94
95_K99_D0.8160.94
57_I62_S0.8150.94
65_H107_S0.8140.94
149_Y182_A0.8110.94
314_K317_I0.8110.94
169_C183_G0.8040.93
105_E166_S0.8000.93
124_R127_I0.7990.93
144_H185_Y0.7880.93
23_E55_K0.7820.92
206_S248_K0.7800.92
325_L328_S0.7790.92
85_E89_K0.7790.92
21_S293_H0.7710.92
12_D224_V0.7710.92
325_L340_I0.7700.92
232_V236_V0.7700.92
65_H135_N0.7690.92
65_H105_E0.7690.92
155_I165_V0.7680.92
335_E339_N0.7650.92
177_T209_E0.7640.91
206_S209_E0.7640.91
7_A37_G0.7630.91
126_A131_A0.7620.91
280_E285_F0.7610.91
321_E325_L0.7580.91
234_R238_E0.7580.91
87_L118_F0.7560.91
105_E190_Q0.7480.91
105_E192_H0.7480.91
117_D158_F0.7460.90
118_F122_A0.7460.90
177_T180_S0.7420.90
96_Y99_D0.7420.90
215_K224_V0.7400.90
18_F45_L0.7380.90
9_L229_L0.7370.90
45_L135_N0.7350.90
264_V298_G0.7330.90
208_E248_K0.7300.90
233_S251_V0.7300.90
195_V232_V0.7290.89
237_A243_P0.7290.89
8_D231_E0.7270.89
76_K80_K0.7270.89
144_H218_L0.7260.89
20_A55_K0.7240.89
44_G61_I0.7230.89
146_F182_A0.7220.89
317_I321_E0.7220.89
152_I188_A0.7180.89
57_I102_M0.7170.89
280_E286_R0.7150.89
89_K93_S0.7150.89
87_L91_Y0.7140.88
45_L107_S0.7130.88
289_A293_H0.7110.88
73_L273_T0.7050.88
29_T42_V0.7020.88
92_E95_K0.6990.87
268_I301_K0.6980.87
32_I35_E0.6980.87
48_S66_L0.6980.87
3_A32_I0.6980.87
230_Y253_D0.6950.87
133_R164_I0.6920.87
57_I101_G0.6920.87
26_F59_T0.6920.87
138_D141_G0.6880.87
17_G29_T0.6870.87
14_I40_V0.6860.86
136_I152_I0.6840.86
64_I101_G0.6830.86
26_F30_R0.6810.86
242_V245_P0.6800.86
215_K222_T0.6800.86
123_I159_V0.6780.86
271_P286_R0.6720.85
13_V43_I0.6710.85
175_L213_G0.6700.85
245_P249_A0.6670.85
94_V126_A0.6670.85
212_M281_E0.6660.85
155_I159_V0.6620.84
270_N273_T0.6610.84
108_P119_L0.6600.84
198_I240_T0.6580.84
116_E150_D0.6560.84
18_F262_I0.6540.84
107_S135_N0.6540.84
39_Q60_G0.6510.83
185_Y218_L0.6510.83
229_L287_R0.6510.83
69_A106_F0.6510.83
41_E61_I0.6500.83
258_H263_H0.6500.83
61_I101_G0.6480.83
236_V240_T0.6470.83
142_T276_P0.6470.83
308_I338_K0.6470.83
73_L270_N0.6450.83
112_T136_I0.6430.83
303_L337_A0.6430.83
261_G295_G0.6410.83
43_I46_S0.6410.83
213_G250_I0.6410.83
195_V208_E0.6400.82
70_T108_P0.6400.82
80_K265_H0.6400.82
335_E338_K0.6380.82
148_Y152_I0.6360.82
303_L308_I0.6350.82
67_F107_S0.6350.82
172_D201_R0.6320.82
71_S74_H0.6300.81
284_N287_R0.6290.81
196_N236_V0.6290.81
66_L93_S0.6290.81
33_F60_G0.6280.81
166_S192_H0.6270.81
88_N91_Y0.6260.81
207_L229_L0.6240.81
21_S292_K0.6210.80
4_R7_A0.6200.80
245_P248_K0.6170.80
69_A79_L0.6160.80
181_L218_L0.6160.80
302_L306_Q0.6140.80
234_R253_D0.6130.80
312_D316_K0.6110.79
61_I64_I0.6100.79
11_V211_V0.6090.79
184_V214_I0.6080.79
108_P134_I0.6070.79
194_T207_L0.6070.79
217_L220_Y0.6060.79
246_Y254_N0.6040.79
46_S67_F0.6040.79
264_V294_S0.6040.79
82_T88_N0.6020.78
69_A107_S0.6010.78
112_T148_Y0.5990.78
41_E63_S0.5990.78
128_E160_G0.5990.78
151_M155_I0.5980.78
66_L94_V0.5980.78
156_V159_V0.5980.78
236_V239_M0.5960.78
143_M147_K0.5950.78
76_K82_T0.5950.78
297_H301_K0.5920.77
108_P136_I0.5920.77
69_A78_K0.5890.77
69_A86_V0.5860.77
9_L211_V0.5860.77
56_T64_I0.5850.76
313_D316_K0.5850.76
326_A332_V0.5850.76
64_I102_M0.5840.76
310_L315_L0.5830.76
122_A241_G0.5830.76
191_A210_V0.5820.76
121_T124_R0.5820.76
53_I96_Y0.5800.76
299_L337_A0.5800.76
89_K92_E0.5790.76
93_S126_A0.5780.76
24_G27_I0.5780.76
171_N245_P0.5760.75
127_I159_V0.5740.75
72_E118_F0.5730.75
29_T33_F0.5730.75
109_E263_H0.5710.75
66_L97_A0.5690.74
196_N251_V0.5650.74
6_L14_I0.5640.74
332_V336_D0.5620.74
259_E292_K0.5620.74
16_A42_V0.5620.74
75_M82_T0.5560.73
133_R190_Q0.5560.73
84_E118_F0.5550.73
116_E120_F0.5550.73
67_F106_F0.5550.73
51_N54_D0.5540.73
69_A114_T0.5520.72
23_E324_K0.5510.72
169_C205_A0.5500.72
83_K114_T0.5500.72
167_V184_V0.5500.72
74_H273_T0.5490.72
120_F159_V0.5480.72
263_H274_Y0.5480.72
94_V106_F0.5440.71
45_L204_N0.5430.71
4_R31_K0.5430.71
94_V125_T0.5430.71
266_G274_Y0.5420.71
212_M277_I0.5400.71
230_Y285_F0.5380.70
208_E212_M0.5380.70
306_Q334_V0.5370.70
180_S184_V0.5370.70
90_I106_F0.5360.70
25_E292_K0.5360.70
218_L221_E0.5350.70
202_A255_A0.5330.70
111_A119_L0.5330.70
177_T193_V0.5330.70
115_E118_F0.5320.69
212_M250_I0.5320.69
120_F160_G0.5320.69
298_G302_L0.5310.69
325_L336_D0.5300.69
94_V131_A0.5300.69
258_H275_E0.5300.69
74_H113_R0.5280.69
173_F276_P0.5280.69
64_I97_A0.5270.69
73_L272_F0.5270.69
18_F22_S0.5250.68
214_I218_L0.5240.68
292_K323_K0.5220.68
105_E133_R0.5200.68
26_F29_T0.5190.68
133_R162_R0.5190.68
269_E300_R0.5180.67
60_G102_M0.5180.67
184_V220_Y0.5180.67
245_P267_V0.5170.67
150_D185_Y0.5170.67
171_N206_S0.5170.67
135_N192_H0.5170.67
140_V276_P0.5170.67
32_I37_G0.5160.67
91_Y122_A0.5140.67
74_H78_K0.5140.67
290_L322_I0.5140.67
7_A35_E0.5130.67
233_S253_D0.5120.67
178_A182_A0.5120.67
185_Y220_Y0.5110.66
62_S102_M0.5110.66
135_N166_S0.5100.66
68_I79_L0.5100.66
123_I151_M0.5090.66
51_N55_K0.5090.66
171_N228_K0.5070.66
279_P287_R0.5040.65
147_K150_D0.5040.65
7_A42_V0.5030.65
112_T116_E0.5030.65
196_N244_V0.5020.65
226_T281_E0.5020.65
68_I86_V0.5020.65
115_E120_F0.5020.65
299_L322_I0.5020.65
173_F213_G0.5010.65
262_I273_T0.5000.65
141_G179_N0.5000.65
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4ov4A 2 0.9625 100 0.153 Contact Map
2ztjA 2 0.804 100 0.169 Contact Map
3ivsA 2 0.9366 100 0.19 Contact Map
3hq1A 2 0.9625 100 0.196 Contact Map
3rmjA 2 0.781 100 0.207 Contact Map
3bleA 2 0.7839 100 0.302 Contact Map
3eegA 2 0.6945 100 0.355 Contact Map
2qf7A 4 0.9481 100 0.431 Contact Map
4qshA 4 0.8646 100 0.448 Contact Map
2cw6A 5 0.7147 100 0.465 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.011 seconds.