Q97W10 - SULSO - GREMLIN Contacts

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Q97W10 - Uncharacterized protein

UniProt:	Q97W10	Sequences: MYADLIEREITQDYKVNLDLKLDNAYIAGAGDSYAVALTIEGKTNGRFKALDPFEGLHYENLDRPLVIVSVSGRPKSNILLATKFKGKTRLYVITANEDSQLARLADYLILIPYKPIIPLPGTLSFLMSLSAVYSLAGEQADEIKESGRLLNLANNPFFVGHKENYGIAYYAMLKFAEIFGYATNSERFEQFCHSPIFMTANRQIVLFRSDNEREKELINSIDYTDTQATDCSGAFCNSIILIKSIVNKMRKEKWDKIYFLENKKILNISSKMIY	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	275 (227)
Sequences:	4808 (2696)
Seq/√Len:	178.9

ECOLI - GLMS AGAS FRLB
BACSU - FRLB GLMS
HALSA - GLMS
SULSO - Q97W10 Q980D3 Q97YQ6 PGMI

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
93_V	106_A	4.573	1.00
83_T	105_L	4.322	1.00
122_G	125_S	3.511	1.00
27_I	37_A	3.501	1.00
86_K	107_D	3.098	1.00
69_V	133_V	3.066	1.00
37_A	69_V	2.949	1.00
68_I	82_A	2.926	1.00
24_N	49_K	2.817	1.00
126_F	168_I	2.790	1.00
84_K	87_G	2.688	1.00
23_D	64_R	2.630	1.00
22_L	65_P	2.589	1.00
189_F	218_L	2.560	1.00
65_P	92_Y	2.346	1.00
82_A	102_L	2.335	1.00
40_I	133_V	2.323	1.00
207_L	222_I	2.310	1.00
173_M	184_T	2.259	1.00
70_S	102_L	2.242	1.00
34_Y	38_L	2.133	1.00
80_L	83_T	2.035	1.00
112_I	128_M	2.003	1.00
177_A	185_N	1.997	1.00
95_T	103_A	1.961	1.00
98_E	111_L	1.936	1.00
84_K	88_K	1.913	1.00
24_N	47_R	1.903	1.00
66_L	89_T	1.903	1.00
74_R	101_Q	1.861	1.00
41_E	49_K	1.854	1.00
52_D	188_R	1.852	1.00
103_A	109_L	1.804	1.00
23_D	63_D	1.770	1.00
155_N	201_A	1.765	1.00
32_D	122_G	1.758	1.00
90_R	108_Y	1.751	1.00
170_Y	186_S	1.742	1.00
28_A	66_L	1.703	1.00
147_S	233_S	1.672	1.00
155_N	203_R	1.669	1.00
25_A	65_P	1.664	1.00
166_Y	170_Y	1.641	1.00
51_L	59_Y	1.637	1.00
26_Y	56_G	1.626	1.00
68_I	91_L	1.618	1.00
27_I	48_F	1.584	1.00
150_L	231_D	1.556	1.00
66_L	85_F	1.555	1.00
76_K	80_L	1.542	1.00
172_A	208_F	1.525	1.00
25_A	67_V	1.466	1.00
45_N	60_E	1.454	1.00
209_R	215_E	1.450	1.00
222_I	232_C	1.414	1.00
91_L	106_A	1.399	1.00
171_Y	175_K	1.373	1.00
143_E	146_E	1.369	1.00
138_G	141_A	1.350	1.00
28_A	56_G	1.330	1.00
38_L	170_Y	1.325	1.00
139_E	142_D	1.316	1.00
90_R	107_D	1.311	1.00
156_N	183_A	1.308	1.00
115_K	124_L	1.294	1.00
85_F	89_T	1.284	1.00
16_V	20_L	1.281	1.00
225_T	230_T	1.274	1.00
86_K	105_L	1.273	1.00
45_N	62_L	1.272	1.00
18_L	90_R	1.253	1.00
145_K	149_R	1.248	1.00
94_I	133_V	1.247	1.00
146_E	149_R	1.244	1.00
147_S	151_L	1.219	1.00
98_E	109_L	1.213	1.00
205_I	230_T	1.211	1.00
30_A	53_P	1.208	1.00
86_K	91_L	1.199	1.00
30_A	75_P	1.187	1.00
223_D	226_D	1.161	1.00
160_V	192_F	1.150	1.00
157_P	176_F	1.150	1.00
27_I	67_V	1.144	1.00
99_D	103_A	1.138	1.00
93_V	102_L	1.124	1.00
209_R	222_I	1.123	1.00
110_I	132_A	1.120	1.00
22_L	90_R	1.118	1.00
40_I	137_A	1.108	1.00
57_L	80_L	1.102	1.00
38_L	50_A	1.102	1.00
93_V	109_L	1.084	1.00
215_E	219_I	1.083	1.00
159_F	176_F	1.080	1.00
68_I	78_N	1.077	1.00
117_I	123_T	1.071	1.00
44_T	48_F	1.069	1.00
112_I	115_K	1.069	1.00
82_A	106_A	1.067	1.00
157_P	182_Y	1.058	1.00
43_K	134_Y	1.054	1.00
137_A	141_A	1.043	1.00
169_A	208_F	1.043	1.00
68_I	85_F	1.031	0.99
112_I	129_S	1.022	0.99
193_C	224_Y	1.008	0.99
32_D	171_Y	1.007	0.99
200_T	230_T	1.004	0.99
205_I	225_T	1.000	0.99
126_F	167_G	0.996	0.99
34_Y	52_D	0.992	0.99
113_P	116_P	0.990	0.99
24_N	63_D	0.980	0.99
200_T	205_I	0.975	0.99
209_R	219_I	0.969	0.99
221_S	224_Y	0.958	0.99
211_D	215_E	0.957	0.99
158_F	204_Q	0.956	0.99
121_P	179_I	0.951	0.99
60_E	84_K	0.941	0.99
236_F	240_I	0.940	0.99
18_L	21_K	0.940	0.99
29_G	33_S	0.939	0.99
112_I	116_P	0.937	0.99
44_T	140_Q	0.936	0.99
165_N	208_F	0.926	0.99
79_I	83_T	0.925	0.99
236_F	239_S	0.923	0.99
20_L	90_R	0.918	0.99
175_K	198_F	0.902	0.99
159_F	206_V	0.899	0.99
192_F	225_T	0.893	0.99
26_Y	66_L	0.891	0.99
99_D	104_R	0.886	0.98
215_E	240_I	0.884	0.98
27_I	50_A	0.878	0.98
193_C	197_I	0.877	0.98
98_E	113_P	0.875	0.98
67_V	92_Y	0.872	0.98
94_I	132_A	0.864	0.98
187_E	196_P	0.864	0.98
224_Y	228_Q	0.860	0.98
163_K	214_R	0.856	0.98
83_T	87_G	0.852	0.98
21_K	64_R	0.851	0.98
21_K	90_R	0.851	0.98
32_D	174_L	0.850	0.98
192_F	197_I	0.849	0.98
181_G	198_F	0.847	0.98
44_T	137_A	0.843	0.98
211_D	237_C	0.833	0.98
30_A	191_Q	0.831	0.98
35_A	170_Y	0.831	0.98
60_E	88_K	0.831	0.98
138_G	142_D	0.830	0.98
71_V	117_I	0.823	0.98
71_V	96_A	0.817	0.97
79_I	101_Q	0.811	0.97
212_N	215_E	0.809	0.97
28_A	68_I	0.804	0.97
25_A	46_G	0.802	0.97
91_L	107_D	0.797	0.97
39_T	168_I	0.793	0.97
33_S	95_T	0.791	0.97
77_S	190_E	0.786	0.97
165_N	210_S	0.786	0.97
82_A	91_L	0.781	0.97
41_E	50_A	0.781	0.97
29_G	50_A	0.779	0.97
117_I	124_L	0.776	0.96
136_L	140_Q	0.776	0.96
60_E	213_E	0.774	0.96
209_R	218_L	0.772	0.96
160_V	207_L	0.770	0.96
101_Q	105_L	0.763	0.96
103_A	111_L	0.762	0.96
226_D	229_A	0.762	0.96
95_T	131_S	0.761	0.96
38_L	167_G	0.756	0.96
168_I	208_F	0.750	0.96
122_G	198_F	0.749	0.96
156_N	204_Q	0.748	0.96
151_L	157_P	0.747	0.96
160_V	225_T	0.746	0.96
34_Y	50_A	0.741	0.95
171_Y	198_F	0.739	0.95
41_E	48_F	0.738	0.95
114_Y	128_M	0.737	0.95
18_L	22_L	0.737	0.95
36_V	133_V	0.735	0.95
146_E	150_L	0.735	0.95
26_Y	49_K	0.727	0.95
35_A	38_L	0.724	0.95
38_L	41_E	0.722	0.95
68_I	102_L	0.719	0.95
143_E	147_S	0.718	0.95
216_K	220_N	0.717	0.95
19_D	22_L	0.717	0.95
110_I	136_L	0.715	0.94
45_N	48_F	0.711	0.94
223_D	227_T	0.709	0.94
17_N	21_K	0.708	0.94
75_P	181_G	0.705	0.94
99_D	109_L	0.701	0.94
207_L	218_L	0.700	0.94
32_D	126_F	0.700	0.94
135_S	139_E	0.695	0.94
71_V	122_G	0.694	0.94
80_L	220_N	0.693	0.93
52_D	191_Q	0.692	0.93
98_E	103_A	0.689	0.93
149_R	152_N	0.684	0.93
52_D	190_E	0.683	0.93
225_T	232_C	0.678	0.93
217_E	220_N	0.676	0.93
39_T	167_G	0.675	0.93
17_N	20_L	0.674	0.93
61_N	84_K	0.670	0.92
123_T	171_Y	0.669	0.92
157_P	206_V	0.663	0.92
32_D	175_K	0.663	0.92
77_S	193_C	0.662	0.92
174_L	195_S	0.662	0.92
218_L	221_S	0.660	0.92
17_N	149_R	0.660	0.92
45_N	61_N	0.657	0.92
130_L	134_Y	0.656	0.92
173_M	186_S	0.656	0.92
219_I	223_D	0.655	0.91
99_D	111_L	0.654	0.91
103_A	106_A	0.650	0.91
96_A	124_L	0.645	0.91
54_F	190_E	0.645	0.91
54_F	162_H	0.642	0.91
171_Y	174_L	0.642	0.91
220_N	224_Y	0.642	0.91
56_G	66_L	0.633	0.90
43_K	137_A	0.632	0.90
135_S	138_G	0.631	0.90
71_V	114_Y	0.629	0.90
144_I	148_G	0.627	0.90
71_V	115_K	0.627	0.90
117_I	120_L	0.624	0.89
72_S	96_A	0.624	0.89
123_T	175_K	0.623	0.89
77_S	96_A	0.623	0.89
18_L	152_N	0.622	0.89
216_K	219_I	0.622	0.89
238_N	241_I	0.622	0.89
137_A	140_Q	0.620	0.89
176_F	180_F	0.618	0.89
159_F	189_F	0.618	0.89
96_A	114_Y	0.617	0.89
71_V	124_L	0.616	0.89
43_K	164_E	0.610	0.88
183_A	199_M	0.610	0.88
27_I	133_V	0.608	0.88
71_V	171_Y	0.606	0.88
80_L	84_K	0.606	0.88
32_D	195_S	0.601	0.88
119_P	125_S	0.598	0.87
129_S	133_V	0.594	0.87
193_C	198_F	0.583	0.86
28_A	85_F	0.582	0.86
154_A	231_D	0.580	0.86
120_L	123_T	0.578	0.85
117_I	121_P	0.577	0.85
16_V	139_E	0.577	0.85
16_V	108_Y	0.573	0.85
94_I	112_I	0.571	0.85
131_S	135_S	0.570	0.85
96_A	122_G	0.569	0.85
95_T	100_S	0.569	0.85
218_L	222_I	0.568	0.85
30_A	52_D	0.567	0.84
83_T	104_R	0.561	0.84
162_H	189_F	0.560	0.84
160_V	189_F	0.560	0.84
22_L	25_A	0.560	0.84
83_T	101_Q	0.555	0.83
192_F	200_T	0.555	0.83
77_S	122_G	0.554	0.83
29_G	37_A	0.554	0.83
34_Y	53_P	0.554	0.83
36_V	40_I	0.553	0.83
143_E	150_L	0.551	0.83
171_Y	195_S	0.550	0.83
75_P	179_I	0.548	0.82
82_A	86_K	0.548	0.82
40_I	43_K	0.547	0.82
59_Y	62_L	0.546	0.82
117_I	195_S	0.546	0.82
222_I	234_G	0.545	0.82
149_R	242_L	0.539	0.81
28_A	81_L	0.537	0.81
167_G	170_Y	0.537	0.81
94_I	110_I	0.534	0.81
131_S	134_Y	0.533	0.81
36_V	175_K	0.533	0.81
78_N	82_A	0.531	0.80
126_F	130_L	0.529	0.80
234_G	237_C	0.527	0.80
57_L	84_K	0.526	0.80
150_L	153_L	0.526	0.80
68_I	106_A	0.524	0.79
25_A	48_F	0.522	0.79
96_A	129_S	0.522	0.79
157_P	204_Q	0.522	0.79
32_D	196_P	0.521	0.79
56_G	85_F	0.519	0.79
200_T	204_Q	0.519	0.79
75_P	120_L	0.518	0.79
159_F	172_A	0.515	0.78
181_G	193_C	0.515	0.78
71_V	190_E	0.515	0.78
102_L	111_L	0.513	0.78
23_D	26_Y	0.513	0.78
19_D	140_Q	0.512	0.78
102_L	106_A	0.511	0.78
177_A	183_A	0.511	0.78
131_S	138_G	0.511	0.78
53_P	75_P	0.510	0.78
101_Q	104_R	0.510	0.78
120_L	124_L	0.510	0.78
24_N	64_R	0.509	0.78
32_D	198_F	0.507	0.77
71_V	77_S	0.506	0.77
79_I	105_L	0.505	0.77
165_N	168_I	0.504	0.77
30_A	55_E	0.504	0.77
117_I	171_Y	0.503	0.77
187_E	191_Q	0.503	0.77
75_P	129_S	0.503	0.77
202_N	229_A	0.503	0.77
167_G	196_P	0.503	0.77
22_L	46_G	0.501	0.76
77_S	181_G	0.501	0.76
217_E	221_S	0.500	0.76
92_Y	110_I	0.500	0.76

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2amlA	2	1	100	0.341	Contact Map
2zj3A	2	0.9927	100	0.348	Contact Map
3tbfA	2	1	100	0.359	Contact Map
1moqA	2	1	100	0.36	Contact Map
3knzA	2	0.9455	100	0.361	Contact Map
4amvA	2	1	100	0.363	Contact Map
2pocA	4	0.9927	100	0.363	Contact Map
4s1wA	2	1	100	0.364	Contact Map
3fj1A	2	1	100	0.364	Contact Map
3g68A	3	0.9964	100	0.366	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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