Q97VZ8 - SULSO - GREMLIN Contacts

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Q97VZ8 - Uncharacterized protein

UniProt:	Q97VZ8	Sequences: MVSRLSTGILDFDKLIQGGIPQGFFIALTGEPGTGKTIFSLHFIAKGLRDGDPCIYVTTEESRDSIIRQAKQFNWDFEEYIEKKLIIIDALMKEKEDQWSLVNLTPEELVNKVIEAKQKLGYGKARLVIDSVSALFLDKPAMARKISYYLKRVLNKWNFTIYATSQYAITTSQAFGFGVEHVADGIIRFRRMIRNGELHRYILIEKMRQTDHDKHVWEIDIVNGKGIVLKGRLEERREDYTLPEKVKRKIIESGKKTEEELK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	262 (207)
Sequences:	10200 (5304)
Seq/√Len:	368.6

ECOLI - DNAB RECA
BACSU - YORI DNAC RECA
HALSA - Q9HP98 Q9HNX8 RADB RADA GVPD2
SULSO - Q97VZ8 Y1861 Q97WB6 RADA

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
117_K	127_L	3.489	1.00
53_P	125_A	3.452	1.00
52_D	125_A	3.446	1.00
6_S	13_D	3.174	1.00
40_S	164_T	2.886	1.00
44_I	128_V	2.864	1.00
47_G	126_R	2.829	1.00
7_T	20_I	2.778	1.00
44_I	54_C	2.498	1.00
26_I	185_G	2.377	1.00
25_F	151_K	2.305	1.00
22_Q	158_N	2.302	1.00
76_D	79_E	2.260	1.00
16_I	185_G	2.252	1.00
22_Q	125_A	2.191	1.00
8_G	13_D	2.176	1.00
188_R	205_E	2.124	1.00
40_S	128_V	2.117	1.00
27_A	183_A	2.113	1.00
20_I	43_F	2.112	1.00
118_Q	157_W	2.065	1.00
24_F	65_S	1.930	1.00
147_S	182_V	1.915	1.00
12_F	187_I	1.859	1.00
187_I	202_I	1.828	1.00
154_L	161_I	1.813	1.00
207_M	210_T	1.768	1.00
30_G	189_F	1.750	1.00
55_I	124_K	1.747	1.00
225_K	228_V	1.707	1.00
46_K	50_D	1.681	1.00
46_K	49_R	1.674	1.00
127_L	161_I	1.673	1.00
41_L	70_A	1.660	1.00
222_V	228_V	1.617	1.00
196_G	216_V	1.594	1.00
114_I	149_Y	1.583	1.00
127_L	159_F	1.566	1.00
53_P	123_G	1.553	1.00
45_A	49_R	1.549	1.00
153_V	157_W	1.537	1.00
44_I	56_Y	1.531	1.00
11_D	14_K	1.518	1.00
26_I	160_T	1.500	1.00
33_G	209_Q	1.487	1.00
20_I	160_T	1.476	1.00
126_R	162_Y	1.471	1.00
116_A	119_K	1.466	1.00
169_I	177_F	1.426	1.00
114_I	153_V	1.426	1.00
110_V	149_Y	1.418	1.00
185_G	207_M	1.386	1.00
10_L	229_L	1.376	1.00
128_V	162_Y	1.368	1.00
12_F	16_I	1.350	1.00
10_L	14_K	1.349	1.00
20_I	162_Y	1.345	1.00
15_L	213_D	1.344	1.00
39_F	43_F	1.331	1.00
103_N	116_A	1.321	1.00
47_G	54_C	1.316	1.00
126_R	160_T	1.316	1.00
7_T	13_D	1.291	1.00
189_F	202_I	1.287	1.00
41_L	66_I	1.263	1.00
201_Y	216_V	1.261	1.00
226_G	229_L	1.254	1.00
220_D	228_V	1.225	1.00
23_G	158_N	1.217	1.00
139_K	142_M	1.208	1.00
3_S	22_Q	1.199	1.00
65_S	69_Q	1.190	1.00
60_E	166_Q	1.186	1.00
67_I	71_K	1.150	1.00
199_H	218_E	1.140	1.00
47_G	52_D	1.133	1.00
53_P	122_Y	1.131	1.00
147_S	163_A	1.128	1.00
30_G	34_T	1.122	1.00
45_A	73_F	1.120	1.00
33_G	206_K	1.119	1.00
3_S	21_P	1.118	1.00
153_V	159_F	1.115	1.00
144_R	178_G	1.098	1.00
25_F	147_S	1.097	1.00
174_A	177_F	1.087	1.00
117_K	157_W	1.087	1.00
222_V	225_K	1.086	1.00
115_E	119_K	1.083	1.00
58_T	66_I	1.067	1.00
48_L	54_C	1.054	1.00
55_I	117_K	1.054	1.00
44_I	48_L	1.053	1.00
207_M	211_D	1.046	1.00
28_L	187_I	1.034	1.00
190_R	203_L	1.034	1.00
109_L	112_K	1.026	1.00
39_F	189_F	1.015	1.00
133_S	165_S	1.014	1.00
140_P	144_R	1.013	1.00
177_F	180_E	0.999	1.00
56_Y	128_V	0.993	1.00
106_P	109_L	0.991	1.00
201_Y	214_K	0.986	1.00
192_M	201_Y	0.986	1.00
39_F	202_I	0.985	1.00
21_P	24_F	0.981	1.00
193_I	198_L	0.975	1.00
61_E	208_R	0.969	1.00
54_C	126_R	0.961	1.00
27_A	186_I	0.959	1.00
132_V	179_V	0.958	1.00
63_R	67_I	0.956	1.00
52_D	126_R	0.955	1.00
43_F	126_R	0.948	1.00
5_L	160_T	0.946	1.00
113_V	135_L	0.944	1.00
183_A	186_I	0.943	1.00
12_F	204_I	0.943	1.00
27_A	179_V	0.940	1.00
112_K	115_E	0.929	1.00
37_T	41_L	0.918	1.00
28_L	39_F	0.917	1.00
112_K	116_A	0.915	1.00
199_H	216_V	0.915	1.00
148_Y	152_R	0.915	1.00
57_V	113_V	0.912	1.00
43_F	47_G	0.911	1.00
149_Y	152_R	0.903	1.00
203_L	214_K	0.893	1.00
20_I	26_I	0.892	1.00
41_L	56_Y	0.891	1.00
9_I	13_D	0.889	1.00
138_D	141_A	0.887	1.00
102_V	106_P	0.885	1.00
38_I	69_Q	0.884	1.00
152_R	156_K	0.882	1.00
25_F	163_A	0.881	1.00
189_F	200_R	0.881	1.00
15_L	204_I	0.878	1.00
114_I	156_K	0.874	1.00
42_H	46_K	0.873	1.00
40_S	162_Y	0.872	1.00
9_I	229_L	0.868	1.00
114_I	118_Q	0.864	1.00
24_F	209_Q	0.863	1.00
38_I	221_I	0.861	1.00
9_I	42_H	0.854	1.00
108_E	112_K	0.846	1.00
55_I	127_L	0.846	1.00
59_T	134_A	0.838	1.00
15_L	217_W	0.832	1.00
105_T	108_E	0.824	1.00
54_C	128_V	0.821	1.00
25_F	150_L	0.818	1.00
9_I	217_W	0.813	1.00
168_A	171_T	0.810	1.00
150_L	161_I	0.810	1.00
57_V	127_L	0.807	1.00
21_P	207_M	0.805	1.00
43_F	46_K	0.805	1.00
11_D	217_W	0.804	1.00
6_S	50_D	0.803	1.00
131_S	164_T	0.797	1.00
62_S	65_S	0.797	1.00
111_N	149_Y	0.793	1.00
173_Q	186_I	0.791	1.00
22_Q	52_D	0.787	1.00
13_D	46_K	0.787	1.00
34_T	200_R	0.786	1.00
117_K	124_K	0.781	1.00
194_R	197_E	0.778	1.00
53_P	124_K	0.776	1.00
152_R	155_N	0.773	1.00
187_I	204_I	0.771	1.00
139_K	175_F	0.767	1.00
166_Q	181_H	0.762	1.00
16_I	19_G	0.760	1.00
48_L	100_S	0.758	1.00
123_G	157_W	0.756	1.00
40_S	56_Y	0.756	1.00
140_P	178_G	0.754	1.00
194_R	199_H	0.754	1.00
100_S	104_L	0.749	1.00
43_F	162_Y	0.749	1.00
226_G	230_K	0.748	1.00
29_T	188_R	0.744	1.00
195_N	198_L	0.742	1.00
26_I	187_I	0.737	1.00
20_I	126_R	0.728	1.00
18_G	21_P	0.726	1.00
174_A	181_H	0.724	1.00
129_I	150_L	0.721	1.00
104_L	108_E	0.720	1.00
74_N	77_F	0.719	1.00
37_T	66_I	0.716	1.00
221_I	226_G	0.716	1.00
33_G	212_H	0.714	1.00
194_R	198_L	0.713	0.99
101_L	104_L	0.712	0.99
145_K	149_Y	0.711	0.99
110_V	146_I	0.706	0.99
23_G	62_S	0.705	0.99
147_S	179_V	0.700	0.99
60_E	208_R	0.699	0.99
28_L	162_Y	0.695	0.99
190_R	201_Y	0.694	0.99
105_T	116_A	0.692	0.99
169_I	180_E	0.691	0.99
175_F	179_V	0.688	0.99
224_G	227_I	0.687	0.99
16_I	187_I	0.685	0.99
15_L	207_M	0.682	0.99
227_I	230_K	0.682	0.99
71_K	78_E	0.682	0.99
225_K	229_L	0.681	0.99
205_E	212_H	0.676	0.99
66_I	69_Q	0.674	0.99
69_Q	209_Q	0.670	0.99
115_E	118_Q	0.659	0.99
107_E	112_K	0.655	0.99
103_N	106_P	0.653	0.99
173_Q	177_F	0.652	0.99
21_P	185_G	0.651	0.99
113_V	129_I	0.650	0.99
218_E	221_I	0.649	0.99
24_F	184_D	0.647	0.99
200_R	214_K	0.645	0.99
4_R	210_T	0.637	0.99
154_L	160_T	0.636	0.99
22_Q	160_T	0.634	0.99
11_D	229_L	0.634	0.99
228_V	231_G	0.631	0.99
153_V	161_I	0.631	0.99
151_K	184_D	0.631	0.99
173_Q	176_G	0.629	0.99
75_W	79_E	0.628	0.99
12_F	202_I	0.628	0.99
42_H	45_A	0.628	0.99
117_K	153_V	0.626	0.99
5_L	47_G	0.621	0.99
69_Q	73_F	0.620	0.99
29_T	173_Q	0.617	0.99
34_T	190_R	0.616	0.99
59_T	135_L	0.613	0.98
9_I	232_R	0.611	0.98
151_K	154_L	0.611	0.98
74_N	78_E	0.609	0.98
184_D	208_R	0.606	0.98
72_Q	80_Y	0.605	0.98
144_R	148_Y	0.605	0.98
129_I	135_L	0.599	0.98
191_R	198_L	0.599	0.98
34_T	171_T	0.599	0.98
143_A	176_G	0.593	0.98
15_L	212_H	0.591	0.98
68_R	71_K	0.590	0.98
56_Y	100_S	0.590	0.98
109_L	113_V	0.587	0.98
202_I	219_I	0.582	0.98
8_G	46_K	0.581	0.98
195_N	199_H	0.581	0.98
24_F	185_G	0.579	0.98
31_E	177_F	0.578	0.98
220_D	224_G	0.577	0.98
20_I	47_G	0.577	0.98
31_E	34_T	0.574	0.98
138_D	142_M	0.574	0.98
202_I	217_W	0.572	0.98
132_V	146_I	0.567	0.97
3_S	18_G	0.565	0.97
213_D	217_W	0.555	0.97
103_N	122_Y	0.553	0.97
45_A	76_D	0.547	0.97
196_G	201_Y	0.546	0.97
40_S	43_F	0.546	0.97
167_Y	177_F	0.543	0.97
191_R	200_R	0.541	0.97
100_S	103_N	0.540	0.97
48_L	80_Y	0.539	0.97
153_V	156_K	0.539	0.97
69_Q	72_Q	0.538	0.97
110_V	135_L	0.538	0.97
196_G	199_H	0.536	0.96
133_S	175_F	0.536	0.96
177_F	181_H	0.535	0.96
31_E	188_R	0.533	0.96
102_V	105_T	0.532	0.96
118_Q	156_K	0.532	0.96
183_A	206_K	0.531	0.96
125_A	160_T	0.531	0.96
65_S	209_Q	0.531	0.96
136_F	152_R	0.530	0.96
75_W	80_Y	0.530	0.96
27_A	163_A	0.529	0.96
176_G	179_V	0.523	0.96
131_S	134_A	0.521	0.96
45_A	70_A	0.517	0.96
135_L	146_I	0.516	0.96
25_F	161_I	0.514	0.96
151_K	163_A	0.512	0.95
218_E	230_K	0.510	0.95
165_S	176_G	0.509	0.95
200_R	221_I	0.508	0.95
131_S	165_S	0.508	0.95
32_P	180_E	0.508	0.95
58_T	61_E	0.505	0.95
16_I	204_I	0.504	0.95
131_S	166_Q	0.503	0.95
179_V	183_A	0.500	0.95

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3bgwA	3	0.874	100	0.352	Contact Map
2dr3A	3	0.9084	100	0.362	Contact Map
2q6tA	1	0.8511	100	0.372	Contact Map
4nmnA	3	0.8702	100	0.374	Contact Map
3bs4A	1	0.8397	100	0.375	Contact Map
1n0wA	1	0.7481	100	0.412	Contact Map
2w0mA	1	0.8282	100	0.414	Contact Map
2cvhA	1	0.7901	100	0.419	Contact Map
2r6aA	5	0.8588	100	0.422	Contact Map
3ldaA	1	0.7634	100	0.423	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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