Q97VZ0 - SULSO - GREMLIN Contacts

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Q97VZ0 - Phosphate permease, putative

UniProt:	Q97VZ0	Sequences: MAITIIISMQNIIEYLLFIVGLISSYVIGANNNATSLGILFATNAIKRRYAYILNMVSILVGVVLGSLTMLHSVYGVVYGNSLYVAIGIFTSLSSSIITFYYLNKSGIPSSLSQTFYPSIAILTLISHGFIELDWVKFWVIVSSWVISPIVAIGFSLLLYLTLNKVVSAETRIIKQITIYKNLILFSSAFTSFIVGANAIGIIVSSALLSAPAYLVIPLYGISAALGVLTSQRIAIRVGFRITKLGYLGASSALIGSNVINEIFTILGIPLSITQTIIGGIVGLSFRSFTSDVRKQVIQIFRSWATSPIFAIILSLAIFGVIKSILGL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	328 (310)
Sequences:	2593 (1389)
Seq/√Len:	78.9

ECOLI - PITA PITB
BACSU - PIT SULP
HALSA - O54523 Q9HN03 Q9HHS6 Q9HRD0 Q9HHW5
SULSO - Q97VZ0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
47_K	50_Y	5.338	1.00
69_T	268_G	4.478	1.00
26_Y	230_T	3.485	1.00
163_L	252_S	3.342	1.00
54_L	189_A	3.179	1.00
317_A	321_V	2.799	1.00
137_K	140_V	2.794	1.00
121_A	138_F	2.781	1.00
126_I	316_L	2.657	1.00
31_N	103_L	2.643	1.00
142_V	146_V	2.610	1.00
95_S	314_L	2.560	1.00
88_G	123_L	2.534	1.00
22_L	219_L	2.529	1.00
22_L	223_S	2.495	1.00
158_L	162_T	2.449	1.00
103_L	110_S	2.364	1.00
46_I	185_L	2.337	1.00
104_N	234_I	2.294	1.00
52_Y	229_L	2.279	1.00
60_L	222_I	2.279	1.00
143_S	147_I	2.259	1.00
20_V	90_F	2.250	1.00
55_N	192_S	2.239	1.00
37_L	41_F	2.216	1.00
78_V	124_T	2.168	1.00
153_I	286_F	2.130	1.00
31_N	104_N	2.098	1.00
40_L	188_S	2.092	1.00
194_I	261_N	2.091	1.00
257_S	277_I	2.089	1.00
104_N	110_S	2.045	1.00
147_I	151_V	1.980	1.00
53_I	57_V	1.961	1.00
98_I	102_Y	1.958	1.00
164_N	288_S	1.945	1.00
72_H	75_Y	1.942	1.00
234_I	237_R	1.925	1.00
197_A	265_T	1.886	1.00
102_Y	106_S	1.873	1.00
100_F	112_L	1.868	1.00
59_I	228_V	1.854	1.00
183_L	251_S	1.853	1.00
31_N	100_F	1.805	1.00
39_I	254_L	1.799	1.00
61_V	193_F	1.792	1.00
45_A	254_L	1.792	1.00
64_V	214_Y	1.783	1.00
114_Q	275_Q	1.754	1.00
95_S	311_A	1.753	1.00
91_T	318_I	1.728	1.00
58_S	189_A	1.725	1.00
125_L	130_F	1.710	1.00
32_N	35_T	1.694	1.00
161_L	165_K	1.675	1.00
190_F	258_N	1.665	1.00
265_T	268_G	1.663	1.00
28_I	112_L	1.656	1.00
17_L	210_S	1.602	1.00
36_S	191_T	1.592	1.00
139_W	142_V	1.571	1.00
180_Y	251_S	1.570	1.00
165_K	168_S	1.547	0.99
261_N	276_T	1.528	0.99
264_F	271_L	1.512	0.99
56_M	225_A	1.508	0.99
31_N	110_S	1.508	0.99
31_N	234_I	1.508	0.99
313_I	317_A	1.502	0.99
108_I	303_S	1.495	0.99
238_V	281_I	1.494	0.99
110_S	234_I	1.483	0.99
226_L	230_T	1.477	0.99
200_I	224_A	1.477	0.99
187_S	255_I	1.474	0.99
15_Y	19_I	1.462	0.99
310_F	314_L	1.461	0.99
62_G	221_G	1.460	0.99
162_T	165_K	1.449	0.99
59_I	195_V	1.446	0.99
159_L	252_S	1.436	0.99
206_S	210_S	1.432	0.99
289_F	294_R	1.427	0.99
312_I	316_L	1.418	0.99
55_N	228_V	1.390	0.99
93_L	193_F	1.385	0.99
31_N	112_L	1.382	0.99
311_A	315_S	1.379	0.99
182_N	186_F	1.364	0.99
25_S	223_S	1.361	0.99
117_Y	141_I	1.360	0.99
238_V	278_I	1.358	0.99
44_N	240_F	1.358	0.99
36_S	250_A	1.351	0.98
146_V	305_A	1.339	0.98
316_L	320_G	1.336	0.98
246_G	288_S	1.325	0.98
315_S	318_I	1.322	0.98
93_L	258_N	1.320	0.98
199_A	274_T	1.302	0.98
99_T	307_S	1.295	0.98
293_V	296_Q	1.290	0.98
52_Y	56_M	1.279	0.98
183_L	186_F	1.275	0.98
242_I	297_V	1.267	0.98
92_S	119_S	1.266	0.98
19_I	150_I	1.264	0.98
246_G	249_G	1.258	0.98
127_S	319_F	1.252	0.98
142_V	310_F	1.251	0.98
39_I	277_I	1.251	0.98
157_L	287_R	1.243	0.97
30_A	104_N	1.239	0.97
257_S	280_G	1.233	0.97
298_I	302_R	1.233	0.97
150_I	154_G	1.216	0.97
125_L	132_E	1.182	0.96
191_T	245_L	1.179	0.96
60_L	225_A	1.178	0.96
119_S	123_L	1.169	0.96
157_L	161_L	1.168	0.96
72_H	76_G	1.157	0.96
237_R	300_I	1.155	0.96
65_L	193_F	1.153	0.96
23_I	101_Y	1.153	0.96
204_V	207_A	1.146	0.96
184_I	247_Y	1.146	0.96
40_L	54_L	1.135	0.96
27_V	101_Y	1.133	0.96
70_M	268_G	1.128	0.95
245_L	253_A	1.125	0.95
46_I	254_L	1.123	0.95
19_I	23_I	1.119	0.95
61_V	65_L	1.119	0.95
24_S	96_S	1.104	0.95
198_N	261_N	1.104	0.95
225_A	228_V	1.103	0.95
100_F	104_N	1.098	0.95
79_Y	82_S	1.092	0.95
184_I	250_A	1.090	0.94
118_P	311_A	1.084	0.94
153_I	279_G	1.070	0.94
173_I	176_Q	1.061	0.94
54_L	58_S	1.034	0.93
188_S	192_S	1.029	0.93
204_V	220_Y	1.026	0.92
193_F	255_I	1.026	0.92
35_T	273_I	1.025	0.92
69_T	72_H	1.024	0.92
158_L	317_A	1.015	0.92
311_A	314_L	1.013	0.92
50_Y	53_I	1.009	0.92
41_F	235_A	1.005	0.92
74_V	205_S	1.004	0.92
278_I	282_V	1.002	0.92
248_L	252_S	1.001	0.92
207_A	220_Y	0.993	0.91
63_V	221_G	0.991	0.91
90_F	322_I	0.973	0.90
114_Q	195_V	0.967	0.90
100_F	103_L	0.967	0.90
20_V	311_A	0.959	0.90
125_L	128_H	0.951	0.89
163_L	167_V	0.951	0.89
92_S	120_I	0.949	0.89
104_N	233_R	0.944	0.89
187_S	254_L	0.941	0.89
277_I	281_I	0.939	0.89
20_V	193_F	0.936	0.89
100_F	110_S	0.934	0.88
35_T	307_S	0.930	0.88
95_S	312_I	0.930	0.88
14_E	210_S	0.930	0.88
27_V	203_I	0.928	0.88
87_I	322_I	0.924	0.88
242_I	281_I	0.918	0.88
172_R	225_A	0.917	0.87
102_Y	314_L	0.914	0.87
57_V	321_V	0.912	0.87
194_I	276_T	0.910	0.87
134_D	137_K	0.909	0.87
261_N	265_T	0.909	0.87
121_A	133_L	0.907	0.87
294_R	297_V	0.906	0.87
41_F	51_A	0.905	0.87
139_W	143_S	0.905	0.87
48_R	232_Q	0.902	0.87
43_T	59_I	0.897	0.86
302_R	306_T	0.893	0.86
171_T	176_Q	0.890	0.86
249_G	268_G	0.889	0.86
133_L	138_F	0.886	0.86
66_G	221_G	0.881	0.85
188_S	191_T	0.881	0.85
33_N	228_V	0.876	0.85
135_W	139_W	0.873	0.85
160_Y	248_L	0.871	0.85
98_I	226_L	0.870	0.85
27_V	100_F	0.869	0.85
57_V	61_V	0.866	0.84
98_I	318_I	0.864	0.84
115_T	307_S	0.864	0.84
35_T	59_I	0.863	0.84
179_I	183_L	0.861	0.84
64_V	218_P	0.861	0.84
35_T	195_V	0.847	0.83
319_F	322_I	0.844	0.83
175_K	218_P	0.843	0.83
30_A	112_L	0.841	0.83
236_I	240_F	0.838	0.82
141_I	198_N	0.831	0.82
123_L	315_S	0.829	0.82
103_L	112_L	0.827	0.81
18_F	95_S	0.823	0.81
17_L	90_F	0.823	0.81
141_I	311_A	0.816	0.81
19_I	212_P	0.816	0.81
244_K	296_Q	0.815	0.81
203_I	220_Y	0.814	0.80
301_F	305_A	0.813	0.80
125_L	158_L	0.811	0.80
30_A	103_L	0.810	0.80
194_I	265_T	0.808	0.80
13_I	321_V	0.807	0.80
54_L	62_G	0.803	0.80
144_S	197_A	0.800	0.79
159_L	255_I	0.799	0.79
133_L	286_F	0.797	0.79
256_G	260_I	0.796	0.79
254_L	277_I	0.792	0.79
77_V	80_G	0.790	0.78
55_N	59_I	0.790	0.78
135_W	218_P	0.788	0.78
96_S	149_P	0.787	0.78
125_L	312_I	0.786	0.78
60_L	64_V	0.785	0.78
130_F	218_P	0.784	0.78
274_T	307_S	0.778	0.77
65_L	224_A	0.777	0.77
195_V	199_A	0.776	0.77
43_T	113_S	0.771	0.77
107_G	233_R	0.771	0.77
70_M	73_S	0.770	0.77
236_I	241_R	0.766	0.76
199_A	273_I	0.766	0.76
158_L	163_L	0.763	0.76
159_L	282_V	0.763	0.76
194_I	198_N	0.760	0.76
172_R	175_K	0.759	0.76
184_I	254_L	0.757	0.75
162_T	166_V	0.751	0.75
104_N	112_L	0.748	0.75
185_L	261_N	0.746	0.74
315_S	319_F	0.745	0.74
98_I	314_L	0.745	0.74
158_L	173_I	0.745	0.74
151_V	260_I	0.745	0.74
92_S	148_S	0.744	0.74
199_A	202_I	0.743	0.74
44_N	49_R	0.743	0.74
237_R	297_V	0.741	0.74
208_L	216_V	0.741	0.74
18_F	22_L	0.739	0.74
115_T	310_F	0.738	0.74
156_S	280_G	0.737	0.73
235_A	242_I	0.735	0.73
118_P	122_I	0.734	0.73
98_I	106_S	0.731	0.73
275_Q	308_P	0.730	0.73
178_T	182_N	0.729	0.73
218_P	222_I	0.727	0.72
65_L	69_T	0.725	0.72
19_I	266_I	0.725	0.72
198_N	265_T	0.717	0.71
100_F	202_I	0.717	0.71
40_L	185_L	0.715	0.71
207_A	210_S	0.715	0.71
96_S	280_G	0.715	0.71
66_G	197_A	0.714	0.71
97_I	219_L	0.713	0.71
103_L	234_I	0.711	0.71
256_G	279_G	0.711	0.71
68_L	217_I	0.710	0.71
219_L	226_L	0.704	0.70
145_W	149_P	0.696	0.69
222_I	226_L	0.688	0.68
191_T	254_L	0.686	0.68
95_S	119_S	0.686	0.68
76_G	133_L	0.686	0.68
141_I	144_S	0.684	0.68
273_I	307_S	0.683	0.68
275_Q	303_S	0.682	0.68
250_A	254_L	0.678	0.67
67_S	217_I	0.677	0.67
64_V	217_I	0.676	0.67
34_A	149_P	0.675	0.67
91_T	322_I	0.673	0.67
136_V	313_I	0.671	0.66
167_V	171_T	0.671	0.66
23_I	27_V	0.671	0.66
167_V	180_Y	0.669	0.66
32_N	274_T	0.669	0.66
24_S	112_L	0.668	0.66
194_I	262_E	0.668	0.66
250_A	273_I	0.660	0.65
252_S	255_I	0.657	0.65
14_E	82_S	0.655	0.64
68_L	204_V	0.647	0.64
191_T	257_S	0.646	0.63
235_A	254_L	0.644	0.63
243_T	288_S	0.640	0.63
67_S	208_L	0.637	0.62
248_L	308_P	0.635	0.62
192_S	279_G	0.635	0.62
156_S	167_V	0.633	0.62
20_V	310_F	0.633	0.62
144_S	148_S	0.630	0.61
271_L	276_T	0.628	0.61
173_I	177_I	0.627	0.61
114_Q	307_S	0.627	0.61
54_L	221_G	0.627	0.61
78_V	84_Y	0.625	0.61
254_L	260_I	0.624	0.61
43_T	250_A	0.621	0.60
174_I	300_I	0.617	0.60
63_V	200_I	0.617	0.60
318_I	322_I	0.616	0.60
302_R	313_I	0.616	0.60
51_A	281_I	0.615	0.60
59_I	114_Q	0.615	0.60
115_T	203_I	0.613	0.59
31_N	149_P	0.612	0.59
25_S	97_I	0.611	0.59
155_F	256_G	0.611	0.59
26_Y	101_Y	0.610	0.59
87_I	150_I	0.607	0.59
30_A	100_F	0.605	0.58
45_A	181_K	0.603	0.58
110_S	115_T	0.599	0.58
14_E	85_V	0.598	0.57
129_G	137_K	0.597	0.57
275_Q	319_F	0.596	0.57
253_A	277_I	0.595	0.57
44_N	181_K	0.594	0.57
194_I	268_G	0.594	0.57
70_M	201_G	0.591	0.57
124_T	130_F	0.590	0.56
144_S	162_T	0.589	0.56
12_I	317_A	0.589	0.56
62_G	191_T	0.589	0.56
66_G	191_T	0.587	0.56
317_A	320_G	0.586	0.56
104_N	202_I	0.584	0.56
69_T	141_I	0.583	0.56
77_V	92_S	0.583	0.56
308_P	311_A	0.582	0.55
108_I	113_S	0.582	0.55
19_I	53_I	0.581	0.55
184_I	195_V	0.580	0.55
125_L	222_I	0.579	0.55
14_E	18_F	0.578	0.55
146_V	255_I	0.578	0.55
68_L	226_L	0.578	0.55
34_A	113_S	0.577	0.55
54_L	185_L	0.576	0.55
94_S	222_I	0.575	0.55
94_S	318_I	0.573	0.54
62_G	145_W	0.573	0.54
187_S	191_T	0.571	0.54
200_I	220_Y	0.571	0.54
121_A	126_I	0.570	0.54
31_N	195_V	0.570	0.54
144_S	274_T	0.570	0.54
21_G	93_L	0.567	0.54
78_V	141_I	0.566	0.53
126_I	320_G	0.566	0.53
143_S	313_I	0.564	0.53
120_I	274_T	0.564	0.53
124_T	128_H	0.564	0.53
37_L	54_L	0.564	0.53
24_S	48_R	0.562	0.53
21_G	34_A	0.561	0.53
84_Y	88_G	0.559	0.53
217_I	221_G	0.558	0.52
40_L	63_V	0.558	0.52
94_S	97_I	0.557	0.52
187_S	258_N	0.557	0.52
82_S	147_I	0.556	0.52
27_V	97_I	0.555	0.52
243_T	285_S	0.554	0.52
190_F	259_V	0.554	0.52
94_S	98_I	0.554	0.52
130_F	321_V	0.553	0.52
123_L	246_G	0.553	0.52
32_N	307_S	0.553	0.52
88_G	189_A	0.552	0.52
12_I	172_R	0.550	0.51
91_T	199_A	0.550	0.51
23_I	97_I	0.549	0.51
63_V	197_A	0.549	0.51
151_V	154_G	0.548	0.51
19_I	106_S	0.548	0.51
292_D	295_K	0.547	0.51
187_S	251_S	0.547	0.51
154_G	291_S	0.545	0.51
280_G	293_V	0.544	0.51
58_S	229_L	0.544	0.51
13_I	322_I	0.542	0.50
152_A	269_I	0.542	0.50
36_S	195_V	0.541	0.50
63_V	210_S	0.540	0.50
19_I	171_T	0.539	0.50
15_Y	18_F	0.538	0.50
96_S	121_A	0.537	0.50
49_R	53_I	0.534	0.49
99_T	231_S	0.534	0.49
217_I	300_I	0.534	0.49
21_G	24_S	0.534	0.49
249_G	288_S	0.533	0.49
40_L	46_I	0.533	0.49
197_A	210_S	0.532	0.49
177_I	252_S	0.531	0.49
30_A	101_Y	0.531	0.49
51_A	285_S	0.530	0.49
171_T	175_K	0.529	0.49
77_V	83_L	0.529	0.49
77_V	85_V	0.529	0.49
99_T	195_V	0.528	0.49
313_I	320_G	0.527	0.48
195_V	231_S	0.527	0.48
202_I	309_I	0.527	0.48
276_T	280_G	0.525	0.48
76_G	204_V	0.524	0.48
200_I	206_S	0.523	0.48
145_W	202_I	0.522	0.48
123_L	189_A	0.521	0.48
253_A	283_G	0.520	0.48
64_V	222_I	0.520	0.48
201_G	204_V	0.519	0.47
31_N	37_L	0.519	0.47
107_G	283_G	0.518	0.47
103_L	118_P	0.518	0.47
131_I	154_G	0.518	0.47
276_T	282_V	0.515	0.47
104_N	113_S	0.512	0.46
57_V	226_L	0.512	0.46
39_I	43_T	0.511	0.46
214_Y	218_P	0.510	0.46
139_W	171_T	0.510	0.46
155_F	259_V	0.510	0.46
73_S	76_G	0.508	0.46
79_Y	136_V	0.508	0.46
84_Y	280_G	0.507	0.46
19_I	158_L	0.505	0.46
117_Y	270_P	0.505	0.46
186_F	259_V	0.505	0.46
131_I	175_K	0.504	0.45
202_I	206_S	0.504	0.45
263_I	267_L	0.504	0.45
252_S	256_G	0.503	0.45
13_I	90_F	0.503	0.45
56_M	192_S	0.503	0.45
54_L	192_S	0.502	0.45
41_F	284_L	0.501	0.45
151_V	158_L	0.500	0.45

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3qe7A	2	0.5	15.2	0.967	Contact Map
4n7wA	2	0.8415	4.7	0.974	Contact Map
3rlbA	1	0.4787	4.5	0.974	Contact Map
1jb0I	1	0.1159	3.4	0.976	Contact Map
4xk8I	1	0.0884	3.2	0.976	Contact Map
4l6vi	1	0.1159	3.1	0.976	Contact Map
4cz8A	2	0.6768	3	0.976	Contact Map
2b2hA	3	0.5091	2.7	0.977	Contact Map
4i7zE	1	0.0854	2.7	0.977	Contact Map
4tkrA	2	0.4756	2.5	0.977	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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