Q97VX4 - SULSO - GREMLIN Contacts

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Q97VX4 - Sugar phosphate nucleotydyl transferase

UniProt:	Q97VX4	Sequences: MYVMHAVILAGGYGKRLRPLTDDRPKPLIEVAGRPIIEWQISWLKQFGITSFVILTGYKWEVLIKWLSENEKRLGISTYFSIEEEPLGTGGALRKVERLLSTENTFIVLNGDIITNLDISKLKISNENVMTMSLVPLKSPYGIVETKDDKIIDFKEKPILENYWINAGVYLMRKEIFKYLPEKGDMEKLTFPKLAKESLLIGIKYYDVYWRSIDTIKDIEEVSEDLIKMKNGLSSER	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	237 (219)
Sequences:	22416 (13937)
Seq/√Len:	941.8

ECOLI - RMLA1 GALF RMLA2 GALU GLMU GLGC
BACSU - RMLA RFBF YNGB GTAB YTDA GLMU GLGC GLGD
HALSA - Q9HQQ4 Q9HSV0 Q9HSZ9 Q9HSZ8 Q9HS61 Q9HSW4
SULSO - Q97VX4 Q980H9 Q7LXE7 Q97U06 Q97XG0 Q97ZD1 Q7LXK9 Q7LXG3 Q980D4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
97_E	181_P	3.337	1.00
34_R	42_S	3.138	1.00
145_E	153_D	2.699	1.00
38_E	66_W	2.694	1.00
145_E	152_I	2.665	1.00
51_S	77_S	2.575	1.00
87_L	91_G	2.542	1.00
5_H	51_S	2.434	1.00
60_W	64_I	2.416	1.00
179_Y	197_E	2.331	1.00
147_K	150_K	2.328	1.00
105_T	173_R	2.326	1.00
51_S	79_Y	2.303	1.00
147_K	152_I	2.301	1.00
6_A	49_I	2.294	1.00
6_A	44_L	2.204	1.00
179_Y	193_K	2.050	1.00
56_T	60_W	2.012	1.00
94_R	97_E	1.978	1.00
41_I	52_F	1.947	1.00
130_M	134_L	1.898	1.00
60_W	82_I	1.878	1.00
193_K	197_E	1.856	1.00
131_T	134_L	1.833	1.00
81_S	95_K	1.824	1.00
192_P	196_K	1.817	1.00
61_E	65_K	1.812	1.00
38_E	42_S	1.805	1.00
153_D	192_P	1.769	1.00
129_V	133_S	1.750	1.00
44_L	109_L	1.740	1.00
18_R	22_D	1.705	1.00
93_L	108_V	1.703	1.00
145_E	162_N	1.694	1.00
10_A	54_I	1.675	1.00
146_T	164_W	1.668	1.00
70_N	74_L	1.666	1.00
193_K	196_K	1.655	1.00
200_L	203_I	1.626	1.00
7_V	96_V	1.621	1.00
24_R	30_E	1.586	1.00
211_R	221_E	1.573	1.00
64_I	82_I	1.568	1.00
200_L	204_K	1.562	1.00
53_V	99_L	1.560	1.00
45_K	76_I	1.553	1.00
62_V	65_K	1.544	1.00
35_P	38_E	1.540	1.00
24_R	62_V	1.532	1.00
123_K	131_T	1.529	1.00
105_T	124_I	1.482	1.00
79_Y	99_L	1.468	1.00
219_I	223_S	1.461	1.00
28_L	62_V	1.445	1.00
189_L	192_P	1.440	1.00
53_V	81_S	1.436	1.00
52_F	76_I	1.425	1.00
135_V	138_K	1.413	1.00
144_V	151_I	1.384	1.00
105_T	171_L	1.376	1.00
91_G	95_K	1.361	1.00
129_V	134_L	1.346	1.00
130_M	133_S	1.341	1.00
104_N	174_K	1.319	1.00
155_K	161_E	1.298	1.00
215_T	218_D	1.292	1.00
199_L	202_G	1.287	1.00
209_Y	225_D	1.283	1.00
110_N	168_G	1.281	1.00
13_Y	86_P	1.259	1.00
94_R	181_P	1.246	1.00
100_L	106_F	1.242	1.00
220_E	224_E	1.234	1.00
178_K	181_P	1.232	1.00
91_G	94_R	1.224	1.00
105_T	174_K	1.215	1.00
183_K	187_E	1.212	1.00
145_E	155_K	1.206	1.00
56_T	64_I	1.200	1.00
175_E	178_K	1.200	1.00
179_Y	182_E	1.196	1.00
53_V	96_V	1.194	1.00
56_T	82_I	1.194	1.00
178_K	182_E	1.166	1.00
107_I	171_L	1.160	1.00
129_V	173_R	1.157	1.00
218_D	221_E	1.145	1.00
136_P	163_Y	1.138	1.00
129_V	199_L	1.137	1.00
49_I	107_I	1.130	1.00
68_S	80_F	1.129	1.00
106_F	174_K	1.128	1.00
56_T	80_F	1.125	1.00
116_N	209_Y	1.114	1.00
38_E	70_N	1.113	1.00
139_S	157_K	1.112	1.00
201_I	204_K	1.110	1.00
213_I	222_V	1.107	1.00
59_K	62_V	1.096	1.00
28_L	66_W	1.096	1.00
188_K	192_P	1.088	1.00
56_T	63_L	1.076	1.00
211_R	225_D	1.076	1.00
182_E	197_E	1.068	1.00
142_G	156_E	1.066	1.00
179_Y	190_T	1.064	1.00
88_G	188_K	1.051	1.00
34_R	39_W	1.043	1.00
37_I	54_I	1.040	1.00
17_L	29_I	1.036	1.00
64_I	80_F	1.026	1.00
182_E	193_K	1.020	1.00
37_I	67_L	1.019	1.00
36_I	213_I	1.016	1.00
172_M	191_F	1.009	1.00
46_Q	117_L	1.003	1.00
11_G	55_L	0.998	1.00
150_K	200_L	0.985	1.00
140_P	218_D	0.983	1.00
37_I	63_L	0.977	1.00
5_H	104_N	0.976	1.00
127_E	173_R	0.971	1.00
53_V	79_Y	0.970	1.00
126_N	201_I	0.968	1.00
128_N	131_T	0.968	1.00
108_V	172_M	0.967	1.00
224_E	227_I	0.966	1.00
130_M	172_M	0.964	1.00
103_E	175_E	0.957	1.00
7_V	106_F	0.956	1.00
94_R	180_L	0.956	1.00
122_L	171_L	0.953	1.00
166_N	170_Y	0.949	1.00
42_S	45_K	0.945	1.00
35_P	66_W	0.945	1.00
128_N	201_I	0.944	1.00
164_W	204_K	0.943	1.00
220_E	223_S	0.943	1.00
81_S	99_L	0.935	1.00
34_R	38_E	0.934	1.00
221_E	224_E	0.934	1.00
17_L	21_T	0.924	1.00
211_R	222_V	0.913	1.00
223_S	227_I	0.911	1.00
4_M	104_N	0.909	1.00
5_H	53_V	0.901	1.00
129_V	132_M	0.895	1.00
42_S	74_L	0.882	1.00
41_I	67_L	0.882	1.00
76_I	79_Y	0.879	1.00
27_P	111_G	0.878	1.00
118_D	121_K	0.877	1.00
140_P	157_K	0.874	1.00
134_L	137_L	0.871	1.00
8_I	111_G	0.870	1.00
119_I	122_L	0.867	1.00
217_K	221_E	0.850	1.00
123_K	126_N	0.850	1.00
30_E	35_P	0.849	1.00
5_H	101_S	0.843	1.00
139_S	159_I	0.843	1.00
47_F	120_S	0.841	1.00
134_L	164_W	0.828	1.00
36_I	40_Q	0.826	1.00
97_E	177_F	0.823	1.00
134_L	144_V	0.823	1.00
113_I	210_W	0.821	1.00
61_E	64_I	0.818	1.00
54_I	78_T	0.817	1.00
41_I	78_T	0.817	1.00
128_N	132_M	0.816	1.00
89_T	110_N	0.814	1.00
128_N	150_K	0.811	1.00
184_G	187_E	0.810	1.00
45_K	52_F	0.810	1.00
221_E	225_D	0.809	1.00
117_L	120_S	0.806	1.00
64_I	68_S	0.800	1.00
143_I	165_I	0.800	1.00
76_I	80_F	0.796	1.00
190_T	193_K	0.795	1.00
21_T	29_I	0.795	1.00
9_L	110_N	0.792	1.00
45_K	75_G	0.791	1.00
29_I	219_I	0.785	1.00
45_K	50_T	0.780	1.00
123_K	128_N	0.779	1.00
122_L	133_S	0.779	1.00
123_K	129_V	0.776	1.00
25_P	62_V	0.775	1.00
32_A	223_S	0.770	1.00
175_E	179_Y	0.767	1.00
150_K	202_G	0.765	1.00
128_N	202_G	0.764	1.00
46_Q	120_S	0.758	1.00
35_P	39_W	0.743	1.00
43_W	117_L	0.740	1.00
7_V	55_L	0.739	1.00
65_K	68_S	0.738	1.00
32_A	39_W	0.733	1.00
220_E	227_I	0.733	1.00
50_T	77_S	0.726	1.00
152_I	155_K	0.720	1.00
93_L	176_I	0.718	1.00
5_H	100_L	0.717	1.00
97_E	106_F	0.716	1.00
81_S	96_V	0.715	1.00
43_W	47_F	0.713	1.00
128_N	133_S	0.710	1.00
109_L	119_I	0.709	1.00
85_E	184_G	0.706	1.00
55_L	92_A	0.705	1.00
75_G	78_T	0.705	1.00
189_L	193_K	0.702	1.00
20_L	216_I	0.700	1.00
33_G	39_W	0.700	1.00
154_F	166_N	0.698	1.00
45_K	70_N	0.696	1.00
103_E	174_K	0.696	1.00
6_A	52_F	0.692	1.00
39_W	226_L	0.692	1.00
79_Y	82_I	0.686	1.00
101_S	174_K	0.683	1.00
67_L	78_T	0.678	1.00
38_E	67_L	0.676	1.00
54_I	67_L	0.673	1.00
30_E	33_G	0.671	1.00
139_S	160_L	0.668	1.00
97_E	100_L	0.668	1.00
20_L	24_R	0.668	1.00
155_K	158_P	0.665	1.00
8_I	109_L	0.662	1.00
14_G	26_K	0.661	1.00
150_K	201_I	0.658	1.00
39_W	42_S	0.656	1.00
100_L	177_F	0.655	1.00
55_L	96_V	0.654	1.00
123_K	133_S	0.654	1.00
122_L	131_T	0.651	1.00
5_H	106_F	0.650	1.00
41_I	54_I	0.647	1.00
28_L	63_L	0.644	1.00
86_P	91_G	0.641	1.00
128_N	200_L	0.641	1.00
41_I	76_I	0.639	1.00
44_L	107_I	0.634	1.00
194_L	198_S	0.633	1.00
134_L	163_Y	0.632	1.00
216_I	220_E	0.631	1.00
212_S	218_D	0.630	1.00
20_L	29_I	0.627	1.00
89_T	166_N	0.626	1.00
7_V	53_V	0.625	1.00
151_I	202_G	0.623	1.00
150_K	199_L	0.623	1.00
109_L	169_V	0.620	1.00
194_L	199_L	0.618	1.00
98_R	101_S	0.616	1.00
97_E	101_S	0.615	1.00
97_E	178_K	0.613	1.00
88_G	189_L	0.610	1.00
124_I	127_E	0.609	1.00
123_K	127_E	0.608	1.00
114_I	222_V	0.605	1.00
57_G	86_P	0.605	1.00
43_W	169_V	0.604	1.00
43_W	46_Q	0.603	1.00
145_E	161_E	0.598	1.00
8_I	40_Q	0.593	1.00
137_L	140_P	0.592	1.00
16_R	26_K	0.589	1.00
9_L	89_T	0.582	1.00
153_D	196_K	0.581	1.00
185_D	188_K	0.579	1.00
217_K	220_E	0.578	1.00
175_E	197_E	0.576	1.00
149_D	204_K	0.573	1.00
31_V	223_S	0.571	1.00
83_E	92_A	0.571	1.00
13_Y	18_R	0.567	1.00
55_L	81_S	0.565	1.00
110_N	113_I	0.562	1.00
192_P	195_A	0.562	1.00
127_E	131_T	0.561	1.00
140_P	215_T	0.559	1.00
88_G	185_D	0.558	1.00
129_V	175_E	0.558	1.00
60_W	63_L	0.558	1.00
39_W	43_W	0.555	1.00
42_S	70_N	0.555	1.00
134_L	202_G	0.555	1.00
96_V	99_L	0.554	1.00
176_I	194_L	0.553	1.00
84_E	95_K	0.553	1.00
132_M	144_V	0.551	1.00
20_L	23_D	0.549	1.00
50_T	75_G	0.546	1.00
179_Y	194_L	0.546	1.00
40_Q	43_W	0.543	1.00
174_K	177_F	0.540	1.00
125_S	128_N	0.535	1.00
115_T	169_V	0.533	1.00
175_E	198_S	0.530	1.00
145_E	163_Y	0.528	1.00
126_N	131_T	0.526	1.00
91_G	186_M	0.526	1.00
190_T	194_L	0.526	1.00
27_P	63_L	0.525	1.00
136_P	162_N	0.524	1.00
137_L	163_Y	0.523	1.00
41_I	45_K	0.522	1.00
124_I	129_V	0.521	1.00
66_W	69_E	0.520	1.00
93_L	177_F	0.520	1.00
25_P	59_K	0.518	1.00
110_N	169_V	0.517	1.00
43_W	119_I	0.516	1.00
85_E	91_G	0.509	1.00
40_Q	109_L	0.508	1.00
175_E	199_L	0.507	1.00
32_A	226_L	0.506	1.00
141_Y	156_E	0.505	1.00
115_T	167_A	0.505	1.00
7_V	93_L	0.503	1.00
87_L	186_M	0.502	1.00
42_S	46_Q	0.502	1.00
188_K	191_F	0.500	1.00
94_R	186_M	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1tzfA	4	0.9578	100	0.326	Contact Map
3jukA	3	0.9536	100	0.327	Contact Map
2e3dA	3	0.9873	100	0.328	Contact Map
2ux8A	4	0.9916	100	0.329	Contact Map
2pa4A	3	0.9916	100	0.343	Contact Map
1iimA	3	0.9916	100	0.349	Contact Map
3brkX	2	0.9705	100	0.359	Contact Map
1yp2A	4	0.9831	100	0.359	Contact Map
4ecmA	3	0.9662	100	0.364	Contact Map
1lvwA	3	0.9873	100	0.367	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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