Q97VW9 - SULSO - GREMLIN Contacts

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Q97VW9 - Uncharacterized protein

UniProt:	Q97VW9	Sequences: MTRILIIASGGGHTGFARAIAQYLPEKVDFVIPTNDPYSRQMISQYANKIYEIPKGREPDESSLVLFKRLFSIIVKSASIKKYDLIIATGSNHSIFPSFFQFLKGGKVYGIESQDRLITKGKAIGIISNYAKGVFLHWQEQRKLYEKKGIVVGPIVEKPKYESKDEGYILVTTGSMGFKRLFDAIVKSVIGKRIVIQTGKIDPTIYRNQNVTTFSFDPDLEKWIANASLVITHQGKTAMEAVVMYRKPVIMVYNNDWKSATTLSDAKKYAEILGATFLSDPITWSDYNVLLEAIENANEPNVFDTGTPNLVKHVLSEL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	318 (301)
Sequences:	4287 (3081)
Seq/√Len:	177.6

BACSU - MURG UGTP
SULSO - Q97VW9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
260_A	263_L	3.696	1.00
270_A	277_F	3.084	1.00
267_K	271_E	3.007	1.00
23_Y	27_K	2.805	1.00
23_Y	26_E	2.784	1.00
219_D	222_K	2.748	1.00
15_G	216_F	2.744	1.00
180_R	280_D	2.717	1.00
230_V	242_V	2.619	1.00
263_L	279_S	2.613	1.00
300_P	303_F	2.574	1.00
86_I	101_Q	2.564	1.00
30_F	81_K	2.547	1.00
78_A	103_L	2.504	1.00
279_S	282_I	2.427	1.00
22_Q	48_N	2.417	1.00
289_V	293_A	2.406	1.00
41_Q	45_Q	2.311	1.00
109_Y	135_F	2.251	1.00
135_F	152_V	2.199	1.00
254_N	257_W	2.170	1.00
267_K	270_A	2.096	1.00
95_I	123_A	2.009	1.00
98_S	108_V	1.961	1.00
169_I	231_I	1.955	1.00
58_E	61_E	1.921	1.00
45_Q	218_P	1.907	1.00
293_A	296_N	1.900	1.00
32_I	97_P	1.897	1.00
276_T	293_A	1.887	1.00
133_G	150_I	1.879	1.00
136_L	151_V	1.857	1.00
168_Y	193_R	1.847	1.00
197_Q	217_D	1.824	1.00
300_P	304_D	1.817	1.00
263_L	267_K	1.802	1.00
303_F	307_T	1.796	1.00
41_Q	200_K	1.792	1.00
78_A	104_K	1.782	1.00
304_D	307_T	1.768	1.00
305_T	309_N	1.763	1.00
300_P	307_T	1.757	1.00
4_I	32_I	1.727	1.00
113_S	154_P	1.717	1.00
20_I	87_I	1.716	1.00
36_D	40_R	1.689	1.00
301_N	304_D	1.686	1.00
269_Y	275_A	1.684	1.00
102_F	130_Y	1.666	1.00
55_K	58_E	1.662	1.00
57_R	60_D	1.659	1.00
74_I	100_F	1.653	1.00
22_Q	25_P	1.644	1.00
307_T	310_L	1.595	1.00
306_G	310_L	1.576	1.00
28_V	49_K	1.556	1.00
108_V	131_A	1.542	1.00
179_K	202_D	1.525	1.00
285_S	290_L	1.515	1.00
230_V	247_K	1.515	1.00
120_K	144_L	1.507	1.00
181_L	252_V	1.504	1.00
40_R	44_S	1.487	1.00
239_M	251_M	1.487	1.00
166_E	193_R	1.480	1.00
304_D	308_P	1.463	1.00
306_G	309_N	1.462	1.00
102_F	105_G	1.457	1.00
14_T	38_Y	1.455	1.00
4_I	83_Y	1.454	1.00
200_K	215_S	1.436	1.00
162_E	193_R	1.411	1.00
169_I	194_I	1.383	1.00
288_N	292_E	1.382	1.00
50_I	53_I	1.381	1.00
71_F	75_V	1.379	1.00
122_K	258_K	1.370	1.00
11_G	15_G	1.364	1.00
242_V	249_V	1.363	1.00
234_Q	251_M	1.363	1.00
64_L	67_F	1.358	1.00
20_I	89_T	1.344	1.00
168_Y	195_V	1.342	1.00
303_F	306_G	1.340	1.00
301_N	305_T	1.336	1.00
242_V	251_M	1.330	1.00
302_V	306_G	1.321	1.00
232_T	238_A	1.318	1.00
298_N	303_F	1.299	1.00
287_Y	291_L	1.293	1.00
99_F	103_L	1.291	1.00
5_L	17_A	1.281	1.00
195_V	226_N	1.275	1.00
184_A	288_N	1.273	1.00
56_G	60_D	1.267	1.00
136_L	141_Q	1.261	1.00
183_D	186_V	1.260	1.00
56_G	59_P	1.256	1.00
22_Q	46_Y	1.242	1.00
195_V	214_F	1.232	1.00
296_N	303_F	1.223	1.00
264_S	267_K	1.223	1.00
276_T	303_F	1.219	1.00
137_H	154_P	1.216	1.00
72_S	76_K	1.200	1.00
282_I	289_V	1.177	1.00
293_A	297_A	1.175	1.00
308_P	311_V	1.174	1.00
85_L	109_Y	1.173	1.00
157_E	226_N	1.169	1.00
184_A	291_L	1.167	1.00
269_Y	274_G	1.164	1.00
3_R	85_L	1.161	1.00
62_S	65_V	1.152	1.00
140_E	271_E	1.145	1.00
260_A	279_S	1.145	1.00
30_F	51_Y	1.144	1.00
55_K	60_D	1.142	1.00
4_I	81_K	1.140	1.00
276_T	296_N	1.134	1.00
195_V	223_W	1.132	1.00
65_V	69_R	1.132	1.00
156_V	244_M	1.131	1.00
5_L	21_A	1.122	1.00
172_T	238_A	1.115	1.00
251_M	275_A	1.115	1.00
33_P	39_S	1.114	1.00
198_T	206_Y	1.109	1.00
167_G	298_N	1.109	1.00
98_S	127_I	1.105	1.00
157_E	162_E	1.096	1.00
270_A	276_T	1.095	1.00
94_S	123_A	1.090	1.00
163_S	193_R	1.085	1.00
139_Q	151_V	1.083	1.00
197_Q	216_F	1.081	1.00
287_Y	290_L	1.077	1.00
195_V	212_T	1.076	1.00
137_H	240_E	1.076	1.00
170_L	223_W	1.071	1.00
258_K	261_T	1.071	1.00
51_Y	76_K	1.070	1.00
57_R	61_E	1.067	1.00
163_S	166_E	1.065	1.00
302_V	305_T	1.063	1.00
250_I	276_T	1.061	1.00
34_T	40_R	1.060	1.00
263_L	277_F	1.059	1.00
203_P	215_S	1.059	1.00
67_F	71_F	1.052	1.00
298_N	304_D	1.050	1.00
249_V	275_A	1.046	1.00
217_D	223_W	1.043	1.00
142_R	149_G	1.033	0.99
74_I	99_F	1.032	0.99
3_R	24_L	1.030	0.99
297_A	300_P	1.029	0.99
7_I	89_T	1.027	0.99
243_V	269_Y	1.026	0.99
168_Y	226_N	1.023	0.99
276_T	279_S	1.022	0.99
66_L	70_L	1.020	0.99
292_E	296_N	1.016	0.99
157_E	161_Y	1.012	0.99
75_V	78_A	1.010	0.99
100_F	104_K	1.010	0.99
56_G	61_E	1.007	0.99
238_A	242_V	1.002	0.99
75_V	103_L	1.000	0.99
123_A	127_I	0.999	0.99
202_D	205_I	0.999	0.99
54_P	76_K	0.998	0.99
182_F	188_S	0.995	0.99
278_L	289_V	0.993	0.99
273_L	310_L	0.992	0.99
60_D	63_S	0.990	0.99
136_L	142_R	0.988	0.99
304_D	310_L	0.985	0.99
255_N	280_D	0.980	0.99
139_Q	142_R	0.976	0.99
172_T	220_L	0.965	0.99
299_E	302_V	0.962	0.99
193_R	212_T	0.960	0.99
307_T	311_V	0.952	0.99
164_K	193_R	0.947	0.99
31_V	48_N	0.945	0.99
196_I	211_V	0.943	0.99
50_I	54_P	0.940	0.99
99_F	130_Y	0.937	0.99
32_I	80_I	0.931	0.99
201_I	215_S	0.930	0.99
252_V	278_L	0.929	0.99
119_T	264_S	0.926	0.99
184_A	188_S	0.924	0.99
299_E	310_L	0.922	0.99
44_S	47_A	0.921	0.99
166_E	210_N	0.921	0.99
267_K	277_F	0.921	0.99
107_K	132_K	0.916	0.99
37_P	40_R	0.915	0.99
120_K	141_Q	0.913	0.99
75_V	79_S	0.909	0.99
14_T	42_M	0.904	0.99
128_S	134_V	0.904	0.99
221_E	224_I	0.903	0.99
17_A	89_T	0.898	0.99
140_E	268_K	0.896	0.99
162_E	168_Y	0.894	0.99
289_V	292_E	0.894	0.99
300_P	310_L	0.882	0.98
102_F	131_A	0.881	0.98
296_N	300_P	0.879	0.98
103_L	130_Y	0.875	0.98
102_F	108_V	0.875	0.98
154_P	237_T	0.873	0.98
21_A	48_N	0.873	0.98
254_N	280_D	0.872	0.98
300_P	306_G	0.872	0.98
198_T	213_T	0.867	0.98
88_A	98_S	0.866	0.98
240_E	243_V	0.866	0.98
159_P	219_D	0.865	0.98
110_G	131_A	0.862	0.98
301_N	308_P	0.861	0.98
137_H	141_Q	0.860	0.98
229_L	304_D	0.860	0.98
300_P	308_P	0.851	0.98
136_L	149_G	0.850	0.98
15_G	42_M	0.848	0.98
182_F	186_V	0.847	0.98
229_L	250_I	0.846	0.98
278_L	285_S	0.845	0.98
184_A	187_K	0.841	0.98
30_F	49_K	0.839	0.98
120_K	145_Y	0.836	0.98
178_F	254_N	0.831	0.98
29_D	48_N	0.831	0.98
286_D	289_V	0.828	0.98
34_T	37_P	0.824	0.97
303_F	310_L	0.822	0.97
231_I	252_V	0.814	0.97
16_F	155_I	0.812	0.97
89_T	111_I	0.807	0.97
137_H	151_V	0.807	0.97
167_G	301_N	0.806	0.97
180_R	255_N	0.805	0.97
239_M	269_Y	0.800	0.97
69_R	72_S	0.795	0.97
178_F	233_H	0.791	0.97
159_P	222_K	0.790	0.97
53_I	73_I	0.789	0.97
89_T	113_S	0.787	0.97
3_R	87_I	0.784	0.97
72_S	78_A	0.783	0.97
35_N	40_R	0.782	0.97
7_I	31_V	0.781	0.97
54_P	58_E	0.780	0.97
7_I	93_H	0.773	0.96
179_K	280_D	0.772	0.96
208_N	211_V	0.772	0.96
186_V	190_I	0.769	0.96
285_S	289_V	0.767	0.96
55_K	61_E	0.767	0.96
5_L	87_I	0.766	0.96
232_T	251_M	0.765	0.96
136_L	152_V	0.765	0.96
257_W	261_T	0.764	0.96
108_V	132_K	0.760	0.96
185_I	188_S	0.758	0.96
229_L	294_I	0.758	0.96
291_L	295_E	0.749	0.96
25_P	29_D	0.745	0.95
257_W	260_A	0.744	0.95
220_L	224_I	0.738	0.95
51_Y	81_K	0.737	0.95
178_F	255_N	0.737	0.95
193_R	210_N	0.736	0.95
139_Q	268_K	0.733	0.95
156_V	245_Y	0.732	0.95
113_S	124_I	0.727	0.95
187_K	190_I	0.725	0.95
186_V	189_V	0.725	0.95
298_N	306_G	0.725	0.95
5_L	24_L	0.725	0.95
157_E	168_Y	0.721	0.95
297_A	304_D	0.715	0.94
298_N	301_N	0.711	0.94
254_N	258_K	0.710	0.94
252_V	280_D	0.709	0.94
290_L	297_A	0.708	0.94
59_P	64_L	0.701	0.94
74_I	103_L	0.700	0.94
41_Q	218_P	0.698	0.94
137_H	237_T	0.697	0.94
203_P	213_T	0.695	0.93
38_Y	42_M	0.695	0.93
139_Q	272_I	0.692	0.93
246_R	311_V	0.692	0.93
299_E	306_G	0.690	0.93
24_L	29_D	0.690	0.93
296_N	299_E	0.688	0.93
172_T	197_Q	0.688	0.93
133_G	148_K	0.687	0.93
145_Y	149_G	0.687	0.93
305_T	308_P	0.685	0.93
4_I	30_F	0.685	0.93
55_K	59_P	0.682	0.93
170_L	247_K	0.681	0.93
6_I	31_V	0.680	0.93
53_I	58_E	0.678	0.93
157_E	160_K	0.676	0.93
28_V	81_K	0.675	0.92
63_S	66_L	0.674	0.92
70_L	73_I	0.672	0.92
301_N	307_T	0.668	0.92
35_N	54_P	0.667	0.92
298_N	307_T	0.666	0.92
304_D	311_V	0.661	0.92
303_F	308_P	0.660	0.92
246_R	308_P	0.658	0.91
163_S	168_Y	0.657	0.91
76_K	79_S	0.654	0.91
158_K	161_Y	0.653	0.91
202_D	256_D	0.652	0.91
112_E	121_G	0.651	0.91
68_K	72_S	0.651	0.91
29_D	33_P	0.649	0.91
180_R	285_S	0.645	0.91
124_I	145_Y	0.643	0.91
4_I	80_I	0.643	0.91
43_I	46_Y	0.639	0.90
53_I	60_D	0.636	0.90
297_A	307_T	0.636	0.90
173_T	178_F	0.635	0.90
33_P	36_D	0.635	0.90
300_P	311_V	0.635	0.90
108_V	127_I	0.634	0.90
5_L	89_T	0.633	0.90
110_G	134_V	0.632	0.90
21_A	29_D	0.628	0.90
111_I	114_Q	0.628	0.90
86_I	106_G	0.627	0.89
297_A	303_F	0.625	0.89
231_I	290_L	0.621	0.89
167_G	228_S	0.621	0.89
138_W	240_E	0.621	0.89
222_K	226_N	0.621	0.89
172_T	232_T	0.620	0.89
188_S	295_E	0.620	0.89
184_A	287_Y	0.620	0.89
34_T	92_N	0.618	0.89
176_M	254_N	0.617	0.89
6_I	98_S	0.616	0.89
31_V	34_T	0.615	0.89
131_A	134_V	0.610	0.88
20_I	135_F	0.608	0.88
6_I	97_P	0.606	0.88
49_K	81_K	0.603	0.88
203_P	206_Y	0.602	0.87
278_L	293_A	0.602	0.87
159_P	165_D	0.601	0.87
239_M	274_G	0.600	0.87
18_R	46_Y	0.597	0.87
51_Y	54_P	0.596	0.87
172_T	230_V	0.595	0.87
43_I	47_A	0.593	0.87
243_V	311_V	0.593	0.87
4_I	86_I	0.591	0.86
158_K	164_K	0.591	0.86
160_K	163_S	0.590	0.86
298_N	310_L	0.589	0.86
233_H	255_N	0.588	0.86
302_V	307_T	0.588	0.86
82_K	106_G	0.587	0.86
270_A	273_L	0.585	0.86
102_F	132_K	0.585	0.86
38_Y	176_M	0.583	0.86
73_I	77_S	0.583	0.86
251_M	269_Y	0.582	0.86
202_D	254_N	0.579	0.85
113_S	137_H	0.579	0.85
78_A	82_K	0.577	0.85
42_M	216_F	0.575	0.85
254_N	261_T	0.571	0.85
250_I	293_A	0.569	0.84
33_P	38_Y	0.568	0.84
183_D	190_I	0.568	0.84
156_V	196_I	0.567	0.84
6_I	94_S	0.566	0.84
65_V	79_S	0.565	0.84
198_T	202_D	0.565	0.84
268_K	272_I	0.562	0.84
171_V	194_I	0.562	0.84
267_K	279_S	0.561	0.84
176_M	233_H	0.558	0.83
7_I	37_P	0.558	0.83
157_E	193_R	0.557	0.83
113_S	236_K	0.555	0.83
224_I	245_Y	0.555	0.83
57_R	63_S	0.552	0.83
183_D	188_S	0.547	0.82
160_K	226_N	0.547	0.82
9_S	92_N	0.547	0.82
29_D	36_D	0.546	0.82
176_M	200_K	0.546	0.82
5_L	29_D	0.545	0.82
169_I	229_L	0.545	0.82
77_S	97_P	0.544	0.82
250_I	278_L	0.543	0.82
190_I	295_E	0.539	0.81
122_K	125_G	0.539	0.81
185_I	190_I	0.538	0.81
114_Q	119_T	0.537	0.81
140_E	143_K	0.537	0.81
54_P	69_R	0.537	0.81
276_T	297_A	0.536	0.81
33_P	44_S	0.536	0.81
110_G	113_S	0.535	0.81
189_V	192_K	0.535	0.81
11_G	216_F	0.533	0.80
202_D	215_S	0.532	0.80
114_Q	175_S	0.531	0.80
70_L	126_I	0.531	0.80
253_Y	262_T	0.530	0.80
66_L	73_I	0.528	0.80
252_V	285_S	0.527	0.80
18_R	22_Q	0.524	0.79
260_A	264_S	0.523	0.79
276_T	307_T	0.522	0.79
266_A	277_F	0.521	0.79
25_P	48_N	0.519	0.79
66_L	77_S	0.517	0.78
71_F	79_S	0.517	0.78
192_K	295_E	0.517	0.78
299_E	303_F	0.515	0.78
306_G	311_V	0.515	0.78
233_H	254_N	0.514	0.78
206_Y	213_T	0.513	0.78
99_F	126_I	0.512	0.78
89_T	93_H	0.510	0.77
72_S	75_V	0.508	0.77
56_G	63_S	0.506	0.77
126_I	130_Y	0.506	0.77
248_P	307_T	0.505	0.77
31_V	35_N	0.503	0.76
302_V	309_N	0.503	0.76
303_F	311_V	0.502	0.76
295_E	299_E	0.502	0.76
223_W	227_A	0.501	0.76
234_Q	239_M	0.500	0.76
299_E	305_T	0.500	0.76
113_S	237_T	0.500	0.76

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1f0kA	2	0.9623	100	0.495	Contact Map
3s2uA	1	0.9214	100	0.5	Contact Map
2acvA	1	0.9528	100	0.526	Contact Map
2c1xA	1	0.956	100	0.533	Contact Map
2vchA	1	0.9591	100	0.537	Contact Map
3hbfA	1	0.9623	100	0.541	Contact Map
4relA	1	0.9497	100	0.553	Contact Map
3otgA	2	0.956	100	0.556	Contact Map
2iyaA	1	0.9434	100	0.556	Contact Map
3rscA	1	0.9623	100	0.56	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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