GREMLIN Database
TPIS - Triosephosphate isomerase
UniProt: Q97VM8 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 227 (212)
Sequences: 302 (219)
Seq/√Len: 15.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
7_I118_I4.4901.00
75_A96_R4.4571.00
89_T117_S4.0411.00
81_V86_A3.8781.00
110_T117_S3.6190.99
209_E212_K3.3440.99
44_P47_M3.2510.99
49_Y53_Q3.1850.99
121_V127_V3.0160.98
214_V218_I2.6390.95
44_P80_M2.5860.95
157_P190_A2.5030.94
89_T110_T2.4810.93
64_V89_T2.3860.92
71_A177_A2.3790.92
148_T182_T2.3430.91
91_I103_I2.3210.91
139_L199_G2.3140.91
99_R102_E2.2570.90
79_E109_R2.2420.89
148_T154_K2.1310.87
213_V216_D2.1310.87
149_G182_T2.1300.87
43_T48_I2.0820.85
8_I214_V2.0440.84
138_I173_I1.9980.83
109_R113_L1.9630.82
150_V155_A1.9440.81
48_I61_A1.9180.80
52_S86_A1.9160.80
148_T151_S1.8840.79
45_A76_I1.7200.72
50_R54_E1.6870.70
78_P109_R1.6630.69
35_Y215_E1.6180.67
33_K56_D1.6140.67
149_G181_I1.5800.65
211_E215_E1.5720.65
22_V51_M1.5430.63
140_I177_A1.5370.63
168_R173_I1.5310.63
66_P102_E1.5060.61
149_G154_K1.4040.56
189_K192_K1.3790.54
139_L176_I1.3780.54
44_P77_L1.3780.54
151_S154_K1.3570.53
216_D219_T1.3310.52
168_R175_L1.3140.51
83_D114_G1.3010.50
25_G55_V1.3000.50
160_I177_A1.3000.50
48_I84_A1.2990.50
177_A195_A1.2800.49
8_I12_A1.2760.49
142_P197_G1.2690.48
67_V77_L1.2530.47
90_L139_L1.2440.47
10_F21_A1.2420.47
64_V77_L1.2240.46
111_K136_D1.2200.46
48_I86_A1.2110.45
101_D123_R1.1990.44
148_T181_I1.1940.44
6_I214_V1.1930.44
200_V214_V1.1840.44
134_K172_G1.1820.43
127_V167_I1.1740.43
10_F25_G1.1570.42
97_R121_V1.1570.42
43_T157_P1.1560.42
114_G209_E1.1550.42
183_T220_S1.1530.42
91_I133_L1.1500.42
97_R101_D1.1440.41
38_E58_P1.1400.41
182_T203_A1.1350.41
20_K214_V1.1270.40
193_L197_G1.1060.39
151_S181_I1.0990.39
68_P112_K1.0930.39
100_A130_F1.0850.38
154_K181_I1.0850.38
63_H142_P1.0840.38
41_L76_I1.0750.38
57_L134_K1.0740.38
6_I198_I1.0730.38
96_R218_I1.0720.37
117_S148_T1.0630.37
184_G216_D1.0570.37
30_K211_E1.0490.36
69_L77_L1.0410.36
133_L218_I1.0400.36
56_D91_I1.0400.36
12_A17_F1.0320.35
152_V193_L1.0230.35
22_V55_V1.0180.35
22_V25_G1.0160.35
131_S173_I0.9990.34
188_Y192_K0.9890.33
104_D188_Y0.9870.33
151_S155_A0.9860.33
94_S139_L0.9750.33
160_I175_L0.9700.32
151_S189_K0.9700.32
107_L208_K0.9620.32
107_L119_L0.9600.32
19_D83_D0.9550.32
40_I60_Y0.9530.31
108_K125_E0.9520.31
40_I88_G0.9500.31
101_D122_D0.9450.31
81_V115_L0.9370.31
116_K136_D0.9340.31
12_A80_M0.9320.30
30_K33_K0.9270.30
68_P77_L0.9230.30
154_K185_E0.9220.30
131_S176_I0.9140.30
152_V157_P0.9080.29
76_I127_V0.9030.29
71_A203_A0.8970.29
94_S201_A0.8910.29
12_A44_P0.8850.28
73_T152_V0.8830.28
149_G153_S0.8800.28
122_D129_P0.8780.28
129_P175_L0.8770.28
59_I65_D0.8700.28
31_I97_R0.8690.28
32_S55_V0.8660.27
19_D117_S0.8650.27
41_L106_V0.8630.27
200_V218_I0.8580.27
134_K165_D0.8450.26
81_V117_S0.8350.26
120_C146_I0.8310.26
78_P113_L0.8300.26
104_D134_K0.8300.26
143_P178_G0.8290.26
44_P191_L0.8270.26
119_L130_F0.8240.26
142_P152_V0.8220.25
73_T94_S0.8200.25
69_L76_I0.8170.25
131_S167_I0.8170.25
205_M209_E0.8150.25
26_K134_K0.8150.25
10_F55_V0.8150.25
20_K23_N0.8140.25
22_V50_R0.8120.25
22_V26_K0.8090.25
157_P193_L0.8070.25
167_I181_I0.8050.25
148_T183_T0.8050.25
47_M127_V0.8030.25
72_F77_L0.8000.25
26_K109_R0.8000.25
152_V159_V0.7980.24
94_S143_P0.7920.24
16_S117_S0.7890.24
22_V186_D0.7890.24
102_E172_G0.7810.24
37_V64_V0.7810.24
73_T157_P0.7810.24
53_Q159_V0.7730.23
97_R103_I0.7720.23
107_L130_F0.7690.23
8_I91_I0.7670.23
139_L200_V0.7650.23
38_E44_P0.7540.23
38_E192_K0.7510.23
143_P201_A0.7500.23
8_I28_I0.7500.23
100_A129_P0.7490.22
64_V81_V0.7490.22
143_P157_P0.7480.22
6_I161_T0.7480.22
50_R216_D0.7480.22
127_V163_A0.7470.22
7_I176_I0.7450.22
8_I200_V0.7430.22
107_L117_S0.7310.22
76_I91_I0.7290.22
52_S110_T0.7270.22
17_F44_P0.7260.22
104_D126_L0.7170.21
94_S97_R0.7150.21
34_E215_E0.7150.21
18_G21_A0.7140.21
120_C199_G0.7120.21
208_K211_E0.7110.21
88_G143_P0.7060.21
12_A188_Y0.7050.21
142_P193_L0.7040.21
45_A209_E0.7010.21
213_V218_I0.7000.21
66_P161_T0.6970.21
36_S39_I0.6960.20
73_T142_P0.6950.20
163_A195_A0.6940.20
68_P108_K0.6940.20
18_G24_L0.6930.20
130_F173_I0.6860.20
140_I190_A0.6800.20
6_I216_D0.6770.20
51_M59_I0.6750.20
51_M82_K0.6710.20
34_E216_D0.6700.20
136_D182_T0.6640.19
24_L214_V0.6600.19
65_D96_R0.6590.19
61_A84_A0.6540.19
5_I10_F0.6530.19
10_F110_T0.6510.19
210_P214_V0.6510.19
188_Y220_S0.6510.19
124_Y137_A0.6490.19
53_Q173_I0.6470.19
64_V90_L0.6460.19
134_K188_Y0.6440.19
88_G97_R0.6440.19
63_H96_R0.6430.19
68_P107_L0.6420.19
106_V215_E0.6370.18
39_I168_R0.6370.18
83_D105_D0.6350.18
145_L179_A0.6340.18
65_D176_I0.6260.18
111_K171_E0.6240.18
16_S88_G0.6230.18
41_L57_L0.6230.18
23_N26_K0.6220.18
73_T143_P0.6190.18
31_I35_Y0.6180.18
171_E175_L0.6180.18
160_I189_K0.6160.18
126_L130_F0.6140.18
35_Y39_I0.6140.18
145_L197_G0.6100.18
129_P132_L0.6080.17
174_Y197_G0.6080.17
152_V179_A0.6050.17
19_D107_L0.6050.17
36_S193_L0.6050.17
30_K111_K0.6040.17
132_L195_A0.6000.17
105_D108_K0.5990.17
73_T90_L0.5980.17
20_K109_R0.5930.17
151_S213_V0.5910.17
203_A213_V0.5900.17
84_A96_R0.5860.17
26_K56_D0.5860.17
72_F198_I0.5850.17
89_T164_V0.5830.17
157_P201_A0.5770.16
101_D105_D0.5770.16
142_P147_G0.5740.16
27_K129_P0.5730.16
173_I206_K0.5730.16
153_S181_I0.5730.16
30_K172_G0.5680.16
130_F138_I0.5680.16
42_S48_I0.5670.16
48_I205_M0.5670.16
33_K209_E0.5660.16
31_I112_K0.5650.16
64_V110_T0.5630.16
47_M77_L0.5610.16
33_K117_S0.5590.16
30_K82_K0.5540.16
76_I96_R0.5530.16
67_V136_D0.5520.16
21_A24_L0.5510.16
51_M55_V0.5500.16
140_I159_V0.5490.16
19_D50_R0.5490.16
114_G183_T0.5460.16
112_K136_D0.5460.16
195_A207_A0.5450.16
68_P72_F0.5450.16
111_K191_L0.5440.15
121_V126_L0.5440.15
134_K168_R0.5410.15
35_Y216_D0.5400.15
43_T73_T0.5400.15
153_S185_E0.5390.15
30_K212_K0.5340.15
26_K212_K0.5340.15
164_V211_E0.5330.15
79_E165_D0.5330.15
85_G171_E0.5320.15
79_E113_L0.5310.15
9_N197_G0.5300.15
60_Y88_G0.5290.15
5_I55_V0.5270.15
105_D109_R0.5250.15
63_H98_L0.5230.15
187_V219_T0.5230.15
101_D126_L0.5210.15
113_L134_K0.5210.15
81_V196_H0.5200.15
45_A196_H0.5180.15
64_V78_P0.5170.15
142_P145_L0.5160.15
80_M195_A0.5160.15
106_V195_A0.5110.15
178_G200_V0.5110.15
24_L207_A0.5110.15
22_V53_Q0.5070.14
66_P195_A0.5070.14
155_A198_I0.5060.14
9_N193_L0.5030.14
42_S143_P0.5020.14
106_V113_L0.5010.14
61_A174_Y0.5010.14
12_A213_V0.5000.14
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4y9aA 2 0.9383 100 0.513 Contact Map
4y96A 2 0.9515 100 0.515 Contact Map
4y8fA 2 0.9295 100 0.524 Contact Map
4y90A 3 0.9339 100 0.526 Contact Map
2h6rA 3 0.9207 100 0.54 Contact Map
4g1kA 2 0.9383 100 0.55 Contact Map
1hg3A 3 0.9736 100 0.557 Contact Map
4mvaA 2 0.9515 100 0.563 Contact Map
1mo0A 2 0.9559 100 0.566 Contact Map
3kxqA 2 0.9075 100 0.567 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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