Q97VH7 - SULSO - GREMLIN Contacts

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Q97VH7 - Uncharacterized protein

UniProt:	Q97VH7	Sequences: MGRKMGISGKLYSFLYLRGFKVWVSYRTQTVLTVLGWVLPVFTYYFTGTALGNRLVNEVGVSNYTAFFTIGLAFQGYVSSVISTISQRLRNEQLYGTIEYYVISRTGTFGFLLYSALWGLTLNTINAIIILAVGFALNIHYNVNIVSAIVIIVLLILSTLGIGMIAGAVTMVTKQGNPISFFFNTFTNLLGGTVFPVTVLPLYVRYISYGIPLTWALEGLREAFLNGAPITDIAPFIIILTIFDIVLLPLGVFSYNHAFKKAREKGTLGEY	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	271 (240)
Sequences:	23535 (16474)
Seq/√Len:	1063.4

ECOLI - YADH YHHJ YBHR YBHS
BACSU - YVFS TAGG YFIM YFIN NATB YHAP
HALSA - Q9HRJ5 Q9HMU9
SULSO - Q97VH7 Q97WP5 Q97U55 Q97Z04 Q7LY08 Q97W41

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
151_I	219_G	5.079	1.00
17_L	21_K	3.965	1.00
155_L	216_A	3.553	1.00
165_I	250_L	3.292	1.00
16_Y	121_T	3.094	1.00
11_L	110_G	3.085	1.00
12_Y	117_L	2.804	1.00
15_L	114_Y	2.758	1.00
19_G	115_S	2.716	1.00
202_L	206_Y	2.669	1.00
128_I	149_I	2.596	1.00
13_S	17_L	2.588	1.00
107_G	110_G	2.572	1.00
63_N	66_A	2.567	1.00
16_Y	117_L	2.410	1.00
158_S	244_D	2.397	1.00
157_L	244_D	2.304	1.00
234_A	238_I	2.239	1.00
92_E	115_S	2.158	1.00
154_L	241_T	2.139	1.00
205_R	209_Y	2.130	1.00
21_K	24_V	2.074	1.00
202_L	205_R	1.987	1.00
165_I	254_S	1.965	1.00
249_P	253_F	1.896	1.00
235_P	239_I	1.894	1.00
215_W	236_F	1.844	1.00
165_I	247_L	1.844	1.00
64_Y	67_F	1.823	1.00
239_I	242_I	1.810	1.00
12_Y	110_G	1.790	1.00
24_V	27_R	1.766	1.00
203_Y	206_Y	1.762	1.00
12_Y	16_Y	1.761	1.00
208_S	214_T	1.745	1.00
147_S	223_A	1.742	1.00
238_I	242_I	1.703	1.00
172_V	175_Q	1.684	1.00
158_S	247_L	1.677	1.00
197_V	208_S	1.657	1.00
161_G	248_L	1.618	1.00
242_I	246_V	1.614	1.00
161_G	244_D	1.608	1.00
253_F	257_H	1.607	1.00
218_E	236_F	1.594	1.00
124_T	153_V	1.593	1.00
133_V	136_A	1.581	1.00
209_Y	215_W	1.577	1.00
215_W	239_I	1.552	1.00
243_F	247_L	1.536	1.00
147_S	233_I	1.506	1.00
206_Y	210_G	1.474	1.00
155_L	159_T	1.458	1.00
170_T	179_I	1.449	1.00
12_Y	113_L	1.449	1.00
235_P	238_I	1.444	1.00
244_D	248_L	1.408	1.00
239_I	243_F	1.402	1.00
151_I	237_I	1.399	1.00
246_V	250_L	1.385	1.00
146_V	150_V	1.372	1.00
206_Y	209_Y	1.370	1.00
158_S	240_L	1.370	1.00
154_L	244_D	1.367	1.00
13_S	16_Y	1.365	1.00
66_A	69_T	1.331	1.00
229_P	232_D	1.309	1.00
154_L	237_I	1.307	1.00
147_S	150_V	1.292	1.00
237_I	241_T	1.286	1.00
166_A	170_T	1.281	1.00
165_I	251_G	1.278	1.00
168_A	254_S	1.267	1.00
16_Y	114_Y	1.260	1.00
151_I	223_A	1.254	1.00
193_T	217_L	1.249	1.00
187_T	211_I	1.249	1.00
132_A	135_F	1.241	1.00
169_V	254_S	1.240	1.00
218_E	222_E	1.228	1.00
209_Y	214_T	1.225	1.00
21_K	25_S	1.223	1.00
20_F	24_V	1.221	1.00
12_Y	114_Y	1.219	1.00
120_L	153_V	1.209	1.00
211_I	214_T	1.207	1.00
131_L	148_A	1.206	1.00
114_Y	118_W	1.201	1.00
18_R	92_E	1.201	1.00
46_F	49_T	1.196	1.00
109_F	113_L	1.196	1.00
91_N	94_L	1.184	1.00
152_I	220_L	1.181	1.00
161_G	247_L	1.168	1.00
246_V	249_P	1.165	1.00
112_L	251_G	1.159	1.00
42_F	46_F	1.157	1.00
45_Y	136_A	1.141	1.00
19_G	22_V	1.140	1.00
214_T	218_E	1.139	1.00
120_L	157_L	1.137	1.00
218_E	232_D	1.128	1.00
123_N	156_I	1.125	1.00
171_M	175_Q	1.114	1.00
14_F	17_L	1.098	1.00
20_F	118_W	1.098	1.00
112_L	252_V	1.094	1.00
158_S	243_F	1.071	1.00
222_E	226_N	1.068	1.00
115_S	119_G	1.067	1.00
250_L	254_S	1.063	1.00
11_L	107_G	1.061	1.00
152_I	216_A	1.058	1.00
36_G	40_P	1.054	1.00
197_V	205_R	1.048	1.00
64_Y	68_F	1.035	1.00
162_I	247_L	1.031	1.00
197_V	214_T	1.030	1.00
69_T	72_L	1.023	1.00
16_Y	118_W	1.020	1.00
16_Y	20_F	0.999	1.00
70_I	75_Q	0.988	1.00
156_I	160_L	0.987	1.00
63_N	67_F	0.984	1.00
232_D	235_P	0.984	1.00
197_V	209_Y	0.980	1.00
200_L	205_R	0.977	1.00
45_Y	137_L	0.977	1.00
163_G	167_G	0.973	1.00
94_L	173_T	0.968	1.00
67_F	71_G	0.967	1.00
38_V	133_V	0.966	1.00
203_Y	207_I	0.964	1.00
146_V	149_I	0.963	1.00
19_G	118_W	0.959	1.00
241_T	245_I	0.954	1.00
62_S	66_A	0.951	1.00
182_F	186_F	0.949	1.00
170_T	175_Q	0.947	1.00
143_V	146_V	0.937	1.00
116_A	161_G	0.926	1.00
161_G	251_G	0.923	1.00
61_V	66_A	0.922	1.00
236_F	240_L	0.920	1.00
150_V	237_I	0.919	1.00
215_W	218_E	0.916	1.00
181_F	185_T	0.916	1.00
231_T	235_P	0.915	1.00
230_I	233_I	0.906	1.00
92_E	97_T	0.906	1.00
241_T	244_D	0.903	1.00
223_A	233_I	0.889	1.00
40_P	44_Y	0.886	1.00
90_R	93_Q	0.885	1.00
150_V	154_L	0.884	1.00
41_V	45_Y	0.868	1.00
162_I	183_F	0.864	1.00
250_L	253_F	0.852	1.00
201_P	205_R	0.850	1.00
116_A	248_L	0.849	1.00
233_I	236_F	0.846	1.00
74_F	213_L	0.841	1.00
109_F	252_V	0.840	1.00
164_M	251_G	0.838	1.00
215_W	219_G	0.836	1.00
142_N	146_V	0.832	1.00
233_I	237_I	0.831	1.00
236_F	239_I	0.829	1.00
72_L	76_G	0.827	1.00
90_R	94_L	0.823	1.00
148_A	220_L	0.821	1.00
20_F	121_T	0.820	1.00
238_I	241_T	0.819	1.00
31_V	35_L	0.813	1.00
219_G	236_F	0.812	1.00
217_L	221_R	0.809	1.00
60_G	64_Y	0.806	1.00
197_V	200_L	0.806	1.00
145_I	149_I	0.801	1.00
124_T	128_I	0.797	1.00
245_I	249_P	0.792	1.00
26_Y	29_Q	0.792	1.00
38_V	42_F	0.786	1.00
69_T	134_G	0.782	1.00
177_N	181_F	0.778	1.00
247_L	251_G	0.777	1.00
42_F	45_Y	0.776	1.00
205_R	208_S	0.775	1.00
216_A	240_L	0.768	1.00
27_R	30_T	0.767	1.00
33_T	37_W	0.760	1.00
144_N	147_S	0.758	1.00
79_S	83_S	0.754	1.00
240_L	244_D	0.750	1.00
42_F	133_V	0.746	1.00
131_L	152_I	0.746	1.00
222_E	228_A	0.746	1.00
106_T	111_F	0.742	1.00
192_G	196_P	0.742	1.00
249_P	252_V	0.740	1.00
62_S	65_T	0.738	1.00
71_G	74_F	0.736	1.00
72_L	75_Q	0.735	1.00
151_I	216_A	0.731	1.00
110_G	114_Y	0.731	1.00
129_I	132_A	0.725	1.00
232_D	236_F	0.724	1.00
41_V	134_G	0.721	1.00
228_A	232_D	0.720	1.00
97_T	100_Y	0.719	1.00
127_A	152_I	0.718	1.00
200_L	208_S	0.718	1.00
215_W	243_F	0.716	1.00
18_R	22_V	0.712	1.00
132_A	145_I	0.711	1.00
88_R	94_L	0.703	1.00
44_Y	48_G	0.701	1.00
222_E	232_D	0.700	1.00
74_F	216_A	0.698	1.00
69_T	73_A	0.692	1.00
128_I	132_A	0.692	1.00
184_N	188_N	0.691	1.00
49_T	136_A	0.684	1.00
149_I	153_V	0.678	1.00
76_G	80_S	0.676	1.00
88_R	93_Q	0.675	1.00
67_F	221_R	0.673	1.00
18_R	100_Y	0.672	1.00
23_W	33_T	0.668	1.00
253_F	256_N	0.667	1.00
119_G	123_N	0.665	1.00
93_Q	171_M	0.659	1.00
221_R	225_L	0.658	1.00
60_G	63_N	0.657	1.00
116_A	160_L	0.655	1.00
41_V	130_I	0.651	1.00
62_S	67_F	0.648	1.00
97_T	101_Y	0.648	1.00
164_M	168_A	0.641	1.00
248_L	252_V	0.639	1.00
71_G	221_R	0.639	1.00
234_A	237_I	0.639	1.00
85_I	119_G	0.639	1.00
252_V	256_N	0.637	1.00
76_G	79_S	0.635	1.00
100_Y	104_S	0.635	1.00
90_R	176_G	0.635	1.00
131_L	145_I	0.634	1.00
80_S	84_T	0.633	1.00
207_I	211_I	0.633	1.00
67_F	72_L	0.632	1.00
138_N	141_Y	0.632	1.00
121_T	125_I	0.631	1.00
231_T	234_A	0.630	1.00
70_I	74_F	0.630	1.00
175_Q	178_P	0.626	1.00
127_A	153_V	0.625	1.00
152_I	156_I	0.624	1.00
187_T	213_L	0.622	1.00
230_I	235_P	0.622	1.00
12_Y	109_F	0.621	1.00
89_L	92_E	0.619	1.00
218_E	228_A	0.618	1.00
174_K	178_P	0.618	1.00
91_N	95_Y	0.618	1.00
166_A	180_S	0.616	1.00
181_F	184_N	0.611	1.00
154_L	240_L	0.611	1.00
244_D	247_L	0.610	1.00
46_F	136_A	0.610	1.00
67_F	74_F	0.609	1.00
65_T	68_F	0.607	1.00
35_L	38_V	0.606	1.00
216_A	219_G	0.602	1.00
73_A	76_G	0.602	1.00
192_G	197_V	0.602	1.00
190_L	204_V	0.600	1.00
113_L	117_L	0.599	1.00
183_F	187_T	0.598	1.00
70_I	73_A	0.597	1.00
168_A	251_G	0.597	1.00
222_E	236_F	0.596	1.00
64_Y	69_T	0.596	1.00
243_F	246_V	0.594	1.00
17_L	20_F	0.593	1.00
74_F	155_L	0.591	1.00
85_I	163_G	0.590	1.00
61_V	67_F	0.590	1.00
43_T	47_T	0.589	1.00
65_T	69_T	0.586	1.00
19_G	119_G	0.585	1.00
183_F	186_F	0.585	1.00
117_L	121_T	0.584	1.00
141_Y	145_I	0.582	1.00
254_S	257_H	0.574	1.00
122_L	126_N	0.574	1.00
67_F	73_A	0.571	1.00
11_L	14_F	0.570	1.00
15_L	110_G	0.568	1.00
32_L	36_G	0.568	1.00
74_F	217_L	0.567	1.00
230_I	234_A	0.567	1.00
128_I	131_L	0.566	1.00
62_S	70_I	0.566	1.00
235_P	242_I	0.566	1.00
101_Y	106_T	0.565	1.00
77_Y	84_T	0.563	1.00
180_S	184_N	0.563	1.00
119_G	160_L	0.562	1.00
92_E	111_F	0.561	1.00
147_S	237_I	0.561	1.00
69_T	74_F	0.560	1.00
88_R	91_N	0.558	1.00
132_A	136_A	0.556	1.00
148_A	223_A	0.556	1.00
100_Y	163_G	0.554	1.00
45_Y	49_T	0.554	1.00
219_G	233_I	0.553	1.00
157_L	161_G	0.550	1.00
78_V	159_T	0.548	1.00
64_Y	70_I	0.547	1.00
43_T	46_F	0.546	1.00
135_F	141_Y	0.545	1.00
165_I	169_V	0.537	1.00
37_W	40_P	0.535	1.00
178_P	181_F	0.535	1.00
120_L	123_N	0.534	1.00
83_S	86_S	0.534	1.00
147_S	151_I	0.533	1.00
196_P	200_L	0.532	1.00
82_I	85_I	0.529	1.00
167_G	176_G	0.529	1.00
89_L	94_L	0.528	1.00
100_Y	106_T	0.527	1.00
96_G	99_E	0.526	1.00
23_W	29_Q	0.524	1.00
210_G	215_W	0.523	1.00
204_V	207_I	0.522	1.00
195_F	199_V	0.520	1.00
102_V	108_T	0.517	1.00
245_I	248_L	0.516	1.00
74_F	77_Y	0.515	1.00
190_L	211_I	0.515	1.00
164_M	167_G	0.514	1.00
166_A	183_F	0.514	1.00
101_Y	104_S	0.512	1.00
222_E	233_I	0.511	1.00
75_Q	81_V	0.511	1.00
98_I	111_F	0.510	1.00
218_E	221_R	0.510	1.00
211_I	215_W	0.510	1.00
185_T	188_N	0.507	1.00
166_A	169_V	0.507	1.00
81_V	159_T	0.506	1.00
153_V	157_L	0.505	1.00
66_A	71_G	0.505	1.00
162_I	165_I	0.505	1.00
120_L	160_L	0.503	1.00
212_P	216_A	0.501	1.00
97_T	111_F	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2m20A	2	0.1513	49.2	0.913	Contact Map
2k1eA	3	0.3653	17.4	0.931	Contact Map
2ks1B	1	0.1624	16.2	0.932	Contact Map
2kluA	1	0.1624	14.5	0.934	Contact Map
2l2tA	2	0.1587	10.6	0.938	Contact Map
4cadC	1	0.738	10.5	0.938	Contact Map
3effK	4	0.3801	9.8	0.938	Contact Map
4wfeA	2	0.6827	9.3	0.939	Contact Map
2jwaA	2	0.1513	8.2	0.941	Contact Map
3wdoA	1	0.797	8	0.941	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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