Q97VG1 - SULSO - GREMLIN Contacts

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Q97VG1 - Muconate cycloisomerase related protein

UniProt:	Q97VG1	Sequences: MTKISEIEAYILGKEVTSAQWASLMVLVRVTTNDGRVGWGETVSALRAEAVANFVKKINTVLKGNDVFNVEKNRLEWYKHDFNMTISLESTTAYSAVDIASWDIIGKELGAPLYKLLGGKTRDKVLVYANGWYQNCVKPEDFAEKAKEIVKMGYKALKFDPFGPYFNDISKKGLDIAEERVKAVREAVGDNVDILIEHHGRFNANSAIMIAKRLEKYNPLFMEEPIHPEDVEGLRKYRNNTSLRIALGERIINKQQALYFMKEGLVDFLQADLYRIGGVTETKKVVGIAETFDVQMAFHNAQGPILNAVTLQFDAFIPNFLIQESFYDWFPSWKRELIYNGTPIDNGYAIIPERPGLGVEVNEKMLDSLKVKGEEYFNPEEPVWVVKGTWRDY	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	393 (340)
Sequences:	11696 (6842)
Seq/√Len:	371.0

ECOLI - DGOD GUDD RSPA GUDX RHMD AEEP MENC
BACSU - GUDD YITF AEEP MENC
HALSA - Q9HS20 Q9HP25 Q9HQN7
SULSO - Q97VG1 GAD ARAD Q97YL9 Q97YR3 Q97UK8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
65_N	76_E	3.614	1.00
124_K	348_Y	3.347	1.00
238_R	267_D	3.234	1.00
259_Y	263_E	3.139	1.00
203_N	206_S	2.950	1.00
235_R	239_N	2.948	1.00
69_N	72_K	2.921	1.00
28_V	96_A	2.710	1.00
258_L	262_K	2.661	1.00
36_R	104_I	2.651	1.00
230_D	233_G	2.592	1.00
52_A	56_K	2.583	1.00
8_E	29_R	2.470	1.00
6_E	31_T	2.455	1.00
31_T	37_V	2.403	1.00
32_T	104_I	2.376	1.00
236_K	239_N	2.339	1.00
8_E	31_T	2.304	1.00
29_R	37_V	2.300	1.00
147_K	187_A	2.269	1.00
212_K	215_E	2.254	1.00
3_K	33_N	2.232	1.00
71_E	284_K	2.213	1.00
212_K	240_N	2.120	1.00
235_R	263_E	2.097	1.00
215_E	242_S	2.096	1.00
211_A	222_M	2.089	1.00
261_M	294_V	2.071	1.00
254_K	281_E	2.030	1.00
236_K	240_N	2.018	1.00
32_T	36_R	2.017	1.00
10_Y	29_R	1.991	1.00
98_D	280_T	1.985	1.00
6_E	59_N	1.960	1.00
179_E	182_K	1.959	1.00
232_E	263_E	1.929	1.00
276_I	285_V	1.928	1.00
211_A	241_T	1.927	1.00
106_G	113_L	1.909	1.00
147_K	151_K	1.908	1.00
268_F	295_Q	1.894	1.00
210_I	214_L	1.878	1.00
36_R	108_E	1.872	1.00
126_L	155_K	1.820	1.00
345_D	350_I	1.806	1.00
12_L	41_E	1.784	1.00
73_N	101_S	1.782	1.00
208_I	240_N	1.780	1.00
193_D	220_L	1.774	1.00
34_D	108_E	1.774	1.00
5_S	31_T	1.746	1.00
139_P	143_A	1.738	1.00
257_A	285_V	1.729	1.00
65_N	72_K	1.711	1.00
182_K	186_E	1.705	1.00
262_K	292_F	1.704	1.00
213_R	216_K	1.702	1.00
7_I	30_V	1.699	1.00
29_R	39_W	1.687	1.00
226_I	237_Y	1.678	1.00
182_K	217_Y	1.656	1.00
138_K	141_D	1.655	1.00
30_V	101_S	1.654	1.00
287_G	290_E	1.602	1.00
42_T	96_A	1.579	1.00
112_P	115_K	1.578	1.00
260_F	266_V	1.563	1.00
150_V	192_V	1.559	1.00
288_I	292_F	1.558	1.00
205_N	236_K	1.552	1.00
36_R	107_K	1.542	1.00
173_G	176_I	1.527	1.00
5_S	33_N	1.526	1.00
84_M	255_Q	1.514	1.00
261_M	288_I	1.506	1.00
183_A	186_E	1.499	1.00
143_A	187_A	1.495	1.00
111_A	115_K	1.491	1.00
124_K	350_I	1.484	1.00
204_A	236_K	1.461	1.00
315_A	351_I	1.450	1.00
178_E	213_R	1.441	1.00
7_I	59_N	1.438	1.00
204_A	237_Y	1.437	1.00
53_N	57_K	1.418	1.00
141_D	144_E	1.414	1.00
113_L	357_L	1.414	1.00
59_N	63_K	1.409	1.00
283_K	287_G	1.408	1.00
176_I	179_E	1.389	1.00
153_G	159_F	1.387	1.00
98_D	102_W	1.382	1.00
148_E	152_M	1.379	1.00
65_N	73_N	1.369	1.00
68_F	108_E	1.366	1.00
261_M	292_F	1.345	1.00
208_I	236_K	1.320	1.00
259_Y	262_K	1.317	1.00
226_I	233_G	1.304	1.00
153_G	156_A	1.303	1.00
57_K	88_L	1.297	1.00
188_V	194_I	1.294	1.00
286_V	317_I	1.291	1.00
32_T	108_E	1.284	1.00
182_K	216_K	1.274	1.00
152_M	195_L	1.271	1.00
27_L	39_W	1.271	1.00
75_L	79_K	1.268	1.00
72_K	76_E	1.264	1.00
111_A	116_L	1.259	1.00
103_D	107_K	1.257	1.00
120_K	123_D	1.256	1.00
77_W	94_Y	1.253	1.00
258_L	288_I	1.250	1.00
282_T	313_F	1.244	1.00
147_K	150_V	1.243	1.00
105_I	109_L	1.232	1.00
195_L	221_F	1.225	1.00
132_W	177_A	1.222	1.00
99_I	279_V	1.219	1.00
209_M	212_K	1.216	1.00
179_E	183_A	1.214	1.00
13_G	22_A	1.211	1.00
257_A	269_L	1.209	1.00
212_K	242_S	1.205	1.00
56_K	59_N	1.205	1.00
340_N	354_R	1.190	1.00
229_E	256_Q	1.189	1.00
157_L	192_V	1.189	1.00
103_D	309_V	1.188	1.00
126_L	348_Y	1.188	1.00
287_G	291_T	1.187	1.00
208_I	237_Y	1.183	1.00
204_A	233_G	1.174	1.00
153_G	157_L	1.168	1.00
195_L	220_L	1.168	1.00
281_E	284_K	1.166	1.00
209_M	213_R	1.164	1.00
283_K	317_I	1.163	1.00
144_E	147_K	1.147	1.00
225_P	245_I	1.132	1.00
235_R	265_L	1.130	1.00
196_I	219_P	1.129	1.00
99_I	309_V	1.127	1.00
225_P	237_Y	1.127	1.00
7_I	62_L	1.124	1.00
237_Y	245_I	1.120	1.00
215_E	243_L	1.116	1.00
156_A	159_F	1.115	1.00
66_D	69_N	1.114	1.00
159_F	184_V	1.114	1.00
106_G	116_L	1.113	1.00
56_K	60_T	1.104	1.00
206_S	209_M	1.101	1.00
33_N	67_V	1.095	1.00
246_A	268_F	1.095	1.00
68_F	105_I	1.094	1.00
68_F	109_L	1.089	1.00
10_Y	27_L	1.089	1.00
254_K	288_I	1.089	1.00
178_E	217_Y	1.083	1.00
256_Q	259_Y	1.083	1.00
160_D	199_H	1.071	1.00
238_R	264_G	1.063	1.00
284_K	290_E	1.056	1.00
61_V	76_E	1.051	1.00
198_H	222_M	1.044	1.00
290_E	318_P	1.043	1.00
207_A	211_A	1.042	1.00
244_R	321_L	1.041	1.00
247_L	260_F	1.036	1.00
264_G	294_V	1.035	1.00
273_L	282_T	1.031	1.00
185_R	189_G	1.028	1.00
144_E	148_E	1.021	1.00
269_L	289_A	1.019	1.00
185_R	194_I	1.019	1.00
202_F	207_A	1.008	1.00
284_K	287_G	1.006	1.00
150_V	157_L	1.006	1.00
71_E	283_K	1.003	1.00
140_E	144_E	0.999	1.00
102_W	280_T	0.998	1.00
187_A	190_D	0.992	1.00
65_N	69_N	0.990	1.00
73_N	76_E	0.982	1.00
272_D	299_H	0.981	1.00
10_Y	39_W	0.979	1.00
224_E	270_Q	0.978	1.00
58_I	97_V	0.976	1.00
27_L	305_I	0.975	1.00
246_A	270_Q	0.973	1.00
183_A	187_A	0.971	1.00
202_F	210_I	0.968	1.00
139_P	179_E	0.967	1.00
146_A	188_V	0.956	1.00
9_A	56_K	0.949	1.00
219_P	243_L	0.931	1.00
184_V	188_V	0.926	1.00
175_D	179_E	0.921	1.00
276_I	282_T	0.915	1.00
49_E	52_A	0.908	1.00
309_V	357_L	0.908	1.00
105_I	116_L	0.906	1.00
266_V	269_L	0.904	1.00
185_R	191_N	0.901	1.00
162_F	177_A	0.901	1.00
70_V	105_I	0.897	1.00
298_F	319_N	0.894	1.00
49_E	57_K	0.893	1.00
273_L	310_T	0.893	1.00
261_M	269_L	0.893	1.00
226_I	234_L	0.889	1.00
109_L	116_L	0.885	1.00
148_E	151_K	0.884	1.00
178_E	182_K	0.883	1.00
232_E	259_Y	0.882	1.00
283_K	316_F	0.882	1.00
143_A	147_K	0.877	1.00
115_K	353_E	0.877	1.00
156_A	195_L	0.873	1.00
146_A	157_L	0.872	1.00
174_L	210_I	0.869	1.00
137_V	141_D	0.869	1.00
253_N	256_Q	0.867	1.00
149_I	152_M	0.867	1.00
102_W	279_V	0.864	1.00
178_E	216_K	0.862	1.00
174_L	178_E	0.860	1.00
205_N	209_M	0.860	1.00
215_E	241_T	0.858	1.00
177_A	180_R	0.855	1.00
9_A	55_V	0.851	1.00
143_A	188_V	0.849	1.00
145_K	148_E	0.845	1.00
55_V	59_N	0.843	1.00
231_V	265_L	0.839	1.00
113_L	279_V	0.838	1.00
117_L	283_K	0.836	1.00
181_V	196_I	0.836	1.00
139_P	183_A	0.836	1.00
154_Y	159_F	0.836	1.00
234_L	247_L	0.829	1.00
41_E	305_I	0.828	1.00
207_A	226_I	0.828	1.00
67_V	101_S	0.827	1.00
258_L	292_F	0.826	1.00
252_I	256_Q	0.825	1.00
248_G	272_D	0.825	1.00
114_Y	312_Q	0.817	1.00
279_V	309_V	0.814	1.00
181_V	217_Y	0.812	1.00
198_H	211_A	0.810	1.00
74_R	98_D	0.810	1.00
239_N	264_G	0.808	1.00
344_I	349_A	0.805	1.00
143_A	146_A	0.804	1.00
251_I	257_A	0.800	1.00
188_V	192_V	0.799	1.00
54_F	93_A	0.796	1.00
208_I	241_T	0.795	1.00
114_Y	351_I	0.795	1.00
82_F	85_T	0.793	1.00
11_I	52_A	0.793	1.00
55_V	60_T	0.791	1.00
234_L	245_I	0.791	1.00
286_V	296_M	0.787	1.00
271_A	282_T	0.785	1.00
153_G	158_K	0.782	1.00
11_I	158_K	0.779	1.00
204_A	208_I	0.778	1.00
78_Y	281_E	0.777	1.00
286_V	298_F	0.777	1.00
74_R	280_T	0.771	1.00
181_V	214_L	0.770	1.00
279_V	283_K	0.770	1.00
49_E	53_N	0.770	1.00
143_A	186_E	0.769	1.00
232_E	235_R	0.769	1.00
298_F	314_D	0.768	1.00
73_N	77_W	0.765	1.00
109_L	112_P	0.764	1.00
110_G	355_P	0.763	1.00
5_S	34_D	0.763	1.00
208_I	226_I	0.760	1.00
247_L	266_V	0.760	1.00
30_V	38_G	0.760	1.00
57_K	60_T	0.758	1.00
288_I	291_T	0.757	1.00
50_A	53_N	0.756	1.00
175_D	213_R	0.755	1.00
62_L	65_N	0.751	1.00
238_R	245_I	0.751	1.00
286_V	290_E	0.747	1.00
251_I	256_Q	0.746	1.00
254_K	284_K	0.746	1.00
202_F	222_M	0.746	1.00
350_I	353_E	0.744	1.00
237_Y	241_T	0.741	1.00
273_L	313_F	0.739	1.00
67_V	108_E	0.738	1.00
53_N	56_K	0.736	1.00
74_R	77_W	0.735	1.00
198_H	202_F	0.735	1.00
190_D	218_N	0.732	1.00
257_A	289_A	0.729	1.00
67_V	105_I	0.728	1.00
52_A	55_V	0.727	1.00
311_L	351_I	0.723	1.00
127_V	130_N	0.723	1.00
85_T	88_L	0.722	1.00
120_K	353_E	0.720	1.00
231_V	260_F	0.718	1.00
295_Q	321_L	0.717	1.00
13_G	24_L	0.717	1.00
311_L	349_A	0.716	1.00
71_E	75_L	0.714	1.00
181_V	188_V	0.713	1.00
315_A	352_P	0.712	1.00
39_W	305_I	0.708	0.99
231_V	259_Y	0.708	0.99
231_V	263_E	0.703	0.99
236_K	264_G	0.703	0.99
31_T	35_G	0.700	0.99
323_Q	326_F	0.699	0.99
9_A	52_A	0.699	0.99
58_I	77_W	0.698	0.99
117_L	316_F	0.697	0.99
71_E	280_T	0.693	0.99
271_A	286_V	0.693	0.99
172_K	176_I	0.692	0.99
117_L	279_V	0.692	0.99
329_W	332_S	0.690	0.99
31_T	39_W	0.689	0.99
3_K	66_D	0.689	0.99
345_D	348_Y	0.688	0.99
102_W	117_L	0.685	0.99
176_I	180_R	0.684	0.99
94_Y	278_G	0.683	0.99
30_V	62_L	0.682	0.99
129_A	149_I	0.681	0.99
102_W	113_L	0.679	0.99
146_A	184_V	0.678	0.99
204_A	226_I	0.676	0.99
143_A	183_A	0.676	0.99
269_L	294_V	0.676	0.99
180_R	185_R	0.675	0.99
69_N	115_K	0.673	0.99
38_G	107_K	0.672	0.99
144_E	187_A	0.671	0.99
74_R	78_Y	0.670	0.99
76_E	79_K	0.670	0.99
61_V	65_N	0.667	0.99
255_Q	258_L	0.666	0.99
4_I	30_V	0.666	0.99
254_K	285_V	0.666	0.99
174_L	177_A	0.664	0.99
70_V	73_N	0.662	0.99
271_A	296_M	0.661	0.99
111_A	119_G	0.659	0.99
330_F	333_W	0.657	0.99
61_V	80_H	0.656	0.99
300_N	307_N	0.656	0.99
177_A	202_F	0.654	0.99
55_V	97_V	0.653	0.99
75_L	284_K	0.653	0.99
290_E	319_N	0.653	0.99
185_R	218_N	0.651	0.99
146_A	198_H	0.648	0.99
40_G	96_A	0.647	0.99
129_A	154_Y	0.646	0.99
211_A	243_L	0.645	0.99
209_M	240_N	0.645	0.99
50_A	57_K	0.645	0.99
254_K	258_L	0.644	0.99
106_G	111_A	0.643	0.99
106_G	109_L	0.641	0.99
106_G	355_P	0.640	0.99
185_R	190_D	0.640	0.99
233_G	236_K	0.639	0.99
156_A	193_D	0.638	0.99
289_A	294_V	0.637	0.99
124_K	346_N	0.637	0.99
270_Q	299_H	0.636	0.99
161_P	198_H	0.635	0.99
257_A	294_V	0.634	0.99
330_F	334_K	0.633	0.99
32_T	67_V	0.630	0.99
309_V	313_F	0.627	0.99
266_V	294_V	0.626	0.99
177_A	185_R	0.623	0.99
326_F	333_W	0.623	0.99
186_E	190_D	0.620	0.99
11_I	46_L	0.620	0.99
257_A	271_A	0.619	0.99
113_L	117_L	0.617	0.99
251_I	260_F	0.615	0.99
101_S	105_I	0.614	0.99
113_L	116_L	0.612	0.99
37_V	107_K	0.612	0.99
234_L	237_Y	0.612	0.99
62_L	101_S	0.609	0.98
114_Y	120_K	0.608	0.98
155_K	191_N	0.607	0.98
314_D	323_Q	0.605	0.98
178_E	188_V	0.605	0.98
255_Q	259_Y	0.598	0.98
140_E	143_A	0.597	0.98
131_G	149_I	0.597	0.98
105_I	108_E	0.594	0.98
104_I	108_E	0.594	0.98
157_L	188_V	0.594	0.98
178_E	181_V	0.594	0.98
84_M	87_S	0.591	0.98
159_F	181_V	0.590	0.98
71_E	123_D	0.590	0.98
7_I	97_V	0.588	0.98
20_Q	23_S	0.587	0.98
207_A	237_Y	0.587	0.98
279_V	313_F	0.585	0.98
314_D	320_F	0.585	0.98
154_Y	158_K	0.584	0.98
283_K	313_F	0.583	0.98
129_A	133_Y	0.580	0.98
78_Y	94_Y	0.580	0.98
322_I	325_S	0.578	0.98
224_E	248_G	0.577	0.98
7_I	28_V	0.574	0.98
221_F	297_A	0.574	0.98
38_G	103_D	0.574	0.98
70_V	280_T	0.573	0.98
343_P	350_I	0.573	0.98
174_L	180_R	0.572	0.98
189_G	192_V	0.572	0.98
150_V	188_V	0.571	0.98
67_V	104_I	0.569	0.98
289_A	296_M	0.569	0.98
308_A	359_V	0.566	0.98
155_K	346_N	0.566	0.98
12_L	305_I	0.563	0.97
224_E	250_R	0.562	0.97
47_R	50_A	0.559	0.97
75_L	78_Y	0.558	0.97
73_N	97_V	0.557	0.97
57_K	61_V	0.556	0.97
70_V	116_L	0.556	0.97
124_K	345_D	0.555	0.97
115_K	120_K	0.555	0.97
173_G	177_A	0.555	0.97
11_I	24_L	0.551	0.97
129_A	152_M	0.550	0.97
176_I	185_R	0.548	0.97
241_T	245_I	0.548	0.97
256_Q	265_L	0.547	0.97
335_R	339_Y	0.546	0.97
40_G	43_V	0.545	0.97
70_V	101_S	0.545	0.97
60_T	63_K	0.545	0.97
119_G	123_D	0.544	0.97
282_T	298_F	0.544	0.97
304_P	338_I	0.543	0.97
274_Y	301_A	0.541	0.97
121_T	318_P	0.539	0.97
286_V	319_N	0.539	0.97
26_V	51_V	0.539	0.97
107_K	110_G	0.539	0.97
143_A	184_V	0.537	0.97
38_G	100_A	0.532	0.96
332_S	336_E	0.531	0.96
173_G	180_R	0.531	0.96
208_I	212_K	0.531	0.96
273_L	279_V	0.531	0.96
161_P	196_I	0.530	0.96
114_Y	353_E	0.528	0.96
42_T	92_T	0.528	0.96
150_V	187_A	0.528	0.96
174_L	185_R	0.528	0.96
128_Y	131_G	0.527	0.96
322_I	326_F	0.527	0.96
211_A	214_L	0.526	0.96
51_V	54_F	0.526	0.96
9_A	26_V	0.526	0.96
311_L	352_P	0.525	0.96
265_L	294_V	0.525	0.96
66_D	115_K	0.524	0.96
103_D	308_A	0.523	0.96
113_L	312_Q	0.523	0.96
194_I	219_P	0.522	0.96
175_D	190_D	0.522	0.96
185_R	188_V	0.520	0.96
290_E	296_M	0.519	0.96
46_L	51_V	0.519	0.96
232_E	236_K	0.519	0.96
62_L	73_N	0.516	0.96
238_R	265_L	0.515	0.96
224_E	249_E	0.515	0.96
31_T	34_D	0.512	0.96
126_L	193_D	0.511	0.96
210_I	213_R	0.511	0.96
146_A	210_I	0.510	0.95
54_F	89_E	0.510	0.95
26_V	52_A	0.510	0.95
161_P	202_F	0.509	0.95
5_S	32_T	0.508	0.95
234_L	266_V	0.507	0.95
33_N	36_R	0.506	0.95
340_N	343_P	0.505	0.95
133_Y	136_C	0.503	0.95
198_H	207_A	0.501	0.95
46_L	50_A	0.500	0.95

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3rcyA	5	0.9669	100	0.211	Contact Map
3bjsA	5	0.9186	100	0.225	Contact Map
3s5sA	2	0.8855	100	0.228	Contact Map
3ozyA	2	0.944	100	0.229	Contact Map
2qdeA	5	0.9084	100	0.23	Contact Map
2qddA	5	0.916	100	0.231	Contact Map
4dwdA	5	0.9389	100	0.232	Contact Map
3rr1A	5	0.9186	100	0.232	Contact Map
3i6eA	4	0.8855	100	0.233	Contact Map
2qgyA	2	0.9313	100	0.235	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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