Q97VF6 - SULSO - GREMLIN Contacts

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Q97VF6 - ABC transporter, permease protein

UniProt:	Q97VF6	Sequences: MVQGKFFEYLRLLWDNKKSRVGLIITVFYILIAIFGQIIFPKTYSLPPSPTTIFMPPQLSNFYLIFGTGPFAESILVQIIQGAKSVIEISFLAGLFATLIGIVVGIIAGYLGGIIDNILMGITDIILTLPSLILIIIIVSAFKTSNPIFLSLILSITSWAGLARAVRSQVLVIRNSPAVEVLRVLGLSRKYIIFREVVPTLGSYIAIHYIFNVEAAVYAEVGLYYLGVLPYNPNNWGAMIQQALSYGAAAGGKAIYYLAFPTIVVAGFMSGLILLSYGIDEISNPRIRRY	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	290 (264)
Sequences:	29088 (18908)
Seq/√Len:	1163.7

ECOLI - DPPC YEJE GSID OPPC SAPC DDPC NIKC NIKB GSIC DDPB DPPB YEJB OPPB SAPB
BACSU - APPC DPPC OPPC DPPB OPPB APPB
HALSA - Q9HMW2 Q9HMI1 Q9HMW3 Q9HMI2 Q9HMX0 Q9HMX1
SULSO - Q97VF6 Q97VK7 Q97YP2 Q97UH2 Q97UG3 Q97YP9 Q97VF8 Q97VK6 Q97UH1 Q97YQ0 Q97YP1 Q97UG4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
56_P	60_S	3.026	1.00
56_P	64_L	2.897	1.00
32_I	61_N	2.758	1.00
170_V	197_V	2.710	1.00
116_D	167_R	2.688	1.00
183_R	189_R	2.399	1.00
173_I	178_A	2.373	1.00
191_Y	195_R	2.318	1.00
104_V	119_L	2.277	1.00
187_L	191_Y	2.265	1.00
173_I	176_S	2.264	1.00
85_S	225_Y	2.251	1.00
92_L	151_S	2.246	1.00
190_K	194_F	2.199	1.00
96_F	155_S	2.182	1.00
50_P	55_M	2.170	1.00
188_S	191_Y	2.166	1.00
24_I	62_F	2.161	1.00
238_A	242_Q	2.090	1.00
177_P	180_E	2.072	1.00
10_L	14_W	2.071	1.00
42_K	46_L	2.010	1.00
179_V	197_V	1.970	1.00
37_Q	49_S	1.959	1.00
179_V	192_I	1.959	1.00
49_S	52_T	1.920	1.00
78_Q	239_M	1.920	1.00
111_L	189_R	1.872	1.00
106_I	170_V	1.859	1.00
210_I	276_S	1.852	1.00
59_L	63_Y	1.846	1.00
111_L	115_I	1.806	1.00
69_G	73_E	1.788	1.00
107_I	111_L	1.785	1.00
99_L	103_V	1.775	1.00
74_S	77_V	1.763	1.00
162_L	212_N	1.746	1.00
25_I	62_F	1.718	1.00
110_Y	189_R	1.707	1.00
178_A	200_T	1.703	1.00
33_A	83_A	1.698	1.00
173_I	201_L	1.693	1.00
114_I	118_I	1.667	1.00
109_G	170_V	1.656	1.00
43_T	46_L	1.605	1.00
66_F	260_F	1.600	1.00
76_L	80_I	1.573	1.00
100_I	159_W	1.568	1.00
174_R	197_V	1.567	1.00
71_F	78_Q	1.561	1.00
100_I	103_V	1.547	1.00
115_I	119_L	1.540	1.00
41_P	44_Y	1.535	1.00
107_I	115_I	1.527	1.00
75_I	260_F	1.511	1.00
151_S	155_S	1.504	1.00
45_S	49_S	1.502	1.00
97_A	158_S	1.496	1.00
88_E	92_L	1.484	1.00
169_Q	172_V	1.475	1.00
119_L	163_A	1.474	1.00
60_S	64_L	1.463	1.00
46_L	49_S	1.463	1.00
110_Y	193_I	1.452	1.00
81_Q	84_K	1.442	1.00
243_A	257_Y	1.441	1.00
98_T	209_Y	1.421	1.00
70_P	242_Q	1.415	1.00
123_T	163_A	1.414	1.00
57_P	60_S	1.402	1.00
101_G	162_L	1.391	1.00
94_G	209_Y	1.381	1.00
240_I	261_P	1.374	1.00
73_E	77_V	1.372	1.00
172_V	176_S	1.368	1.00
181_V	185_L	1.361	1.00
105_G	166_V	1.338	1.00
105_G	109_G	1.335	1.00
21_V	25_I	1.323	1.00
121_G	124_D	1.317	1.00
52_T	55_M	1.314	1.00
19_S	278_G	1.313	1.00
35_F	38_I	1.305	1.00
167_R	171_L	1.303	1.00
101_G	166_V	1.280	1.00
207_I	280_D	1.257	1.00
280_D	284_N	1.253	1.00
173_I	197_V	1.247	1.00
173_I	179_V	1.245	1.00
114_I	117_N	1.234	1.00
108_A	119_L	1.233	1.00
16_N	270_S	1.226	1.00
104_V	163_A	1.225	1.00
133_I	137_I	1.224	1.00
155_S	159_W	1.211	1.00
148_I	152_L	1.210	1.00
62_F	65_I	1.210	1.00
255_I	259_A	1.206	1.00
85_S	88_E	1.205	1.00
109_G	174_R	1.200	1.00
66_F	76_L	1.189	1.00
53_I	56_P	1.178	1.00
138_I	153_I	1.175	1.00
165_A	208_H	1.164	1.00
106_I	193_I	1.163	1.00
206_A	283_S	1.152	1.00
182_L	196_E	1.148	1.00
79_I	264_V	1.148	1.00
23_L	266_A	1.145	1.00
22_G	271_G	1.144	1.00
279_I	282_I	1.143	1.00
89_I	220_E	1.135	1.00
73_E	78_Q	1.108	1.00
31_L	35_F	1.106	1.00
170_V	201_L	1.099	1.00
112_G	174_R	1.094	1.00
73_E	81_Q	1.091	1.00
22_G	278_G	1.086	1.00
182_L	192_I	1.080	1.00
14_W	18_K	1.074	1.00
126_I	160_A	1.068	1.00
102_I	166_V	1.060	1.00
64_L	74_S	1.057	1.00
106_I	198_V	1.056	1.00
36_G	81_Q	1.053	1.00
169_Q	173_I	1.052	1.00
182_L	187_L	1.051	1.00
66_F	79_I	1.049	1.00
50_P	53_I	1.042	1.00
60_S	63_Y	1.042	1.00
166_V	170_V	1.039	1.00
23_L	27_V	1.029	1.00
166_V	205_I	1.028	1.00
17_K	20_R	1.026	1.00
117_N	121_G	1.023	1.00
103_V	106_I	1.021	1.00
46_L	50_P	1.021	1.00
21_V	62_F	1.015	1.00
122_I	126_I	1.011	1.00
180_E	183_R	1.011	1.00
153_I	157_T	1.008	1.00
191_Y	194_F	1.005	1.00
239_M	257_Y	1.003	1.00
26_T	271_G	0.999	1.00
25_I	29_Y	0.999	1.00
146_N	149_F	0.996	1.00
261_P	265_V	0.989	1.00
36_G	39_I	0.987	1.00
75_I	239_M	0.983	1.00
185_L	196_E	0.982	1.00
213_V	272_L	0.981	1.00
129_L	134_L	0.973	1.00
58_Q	63_Y	0.972	1.00
159_W	163_A	0.971	1.00
14_W	20_R	0.971	1.00
93_A	155_S	0.968	1.00
135_I	153_I	0.962	1.00
278_G	282_I	0.959	1.00
75_I	257_Y	0.958	1.00
243_A	258_L	0.957	1.00
48_P	51_T	0.956	1.00
116_D	171_L	0.954	1.00
182_L	185_L	0.954	1.00
256_Y	260_F	0.948	1.00
183_R	192_I	0.946	1.00
40_F	77_V	0.940	1.00
113_G	116_D	0.939	1.00
120_M	163_A	0.938	1.00
166_V	201_L	0.935	1.00
18_K	21_V	0.933	1.00
82_G	239_M	0.926	1.00
84_K	88_E	0.926	1.00
203_S	280_D	0.924	1.00
112_G	175_N	0.921	1.00
78_Q	257_Y	0.921	1.00
260_F	263_I	0.920	1.00
116_D	120_M	0.920	1.00
55_M	58_Q	0.916	1.00
68_T	74_S	0.913	1.00
39_I	45_S	0.907	1.00
77_V	81_Q	0.904	1.00
88_E	147_P	0.904	1.00
118_I	122_I	0.898	1.00
115_I	118_I	0.896	1.00
48_P	52_T	0.895	1.00
241_Q	245_S	0.893	1.00
148_I	151_S	0.892	1.00
187_L	192_I	0.887	1.00
88_E	91_F	0.887	1.00
79_I	260_F	0.884	1.00
271_G	275_L	0.881	1.00
117_N	120_M	0.880	1.00
93_A	97_A	0.872	1.00
56_P	59_L	0.871	1.00
102_I	205_I	0.868	1.00
21_V	24_I	0.866	1.00
189_R	192_I	0.863	1.00
272_L	276_S	0.861	1.00
93_A	154_L	0.859	1.00
131_S	219_A	0.859	1.00
8_E	12_L	0.856	1.00
105_G	170_V	0.846	1.00
142_F	149_F	0.845	1.00
201_L	204_Y	0.838	1.00
24_I	28_F	0.838	1.00
123_T	160_A	0.829	1.00
206_A	276_S	0.824	1.00
113_G	117_N	0.823	1.00
50_P	56_P	0.817	1.00
25_I	271_G	0.815	1.00
28_F	32_I	0.813	1.00
16_N	274_L	0.813	1.00
58_Q	61_N	0.811	1.00
143_K	149_F	0.810	1.00
147_P	151_S	0.804	1.00
98_T	102_I	0.794	1.00
152_L	155_S	0.794	1.00
154_L	219_A	0.794	1.00
99_L	102_I	0.788	1.00
80_I	84_K	0.788	1.00
22_G	26_T	0.786	1.00
168_S	171_L	0.782	1.00
54_F	57_P	0.779	1.00
51_T	55_M	0.779	1.00
109_G	171_L	0.779	1.00
208_H	212_N	0.778	1.00
104_V	159_W	0.778	1.00
92_L	225_Y	0.777	1.00
14_W	17_K	0.775	1.00
42_K	77_V	0.772	1.00
83_A	268_F	0.771	1.00
77_V	80_I	0.771	1.00
98_T	162_L	0.770	1.00
131_S	135_I	0.769	1.00
78_Q	81_Q	0.769	1.00
24_I	65_I	0.766	1.00
189_R	193_I	0.766	1.00
22_G	25_I	0.764	1.00
144_T	149_F	0.762	1.00
11_R	14_W	0.761	1.00
98_T	103_V	0.761	1.00
37_Q	84_K	0.757	1.00
176_S	180_E	0.751	1.00
103_V	107_I	0.750	1.00
239_M	261_P	0.747	1.00
35_F	39_I	0.747	1.00
68_T	72_A	0.746	1.00
42_K	45_S	0.746	1.00
149_F	152_L	0.743	1.00
86_V	90_S	0.743	1.00
20_R	267_G	0.742	1.00
194_F	197_V	0.739	1.00
138_I	149_F	0.739	1.00
92_L	95_L	0.738	1.00
242_Q	245_S	0.737	1.00
170_V	174_R	0.733	1.00
108_A	116_D	0.731	1.00
259_A	263_I	0.730	1.00
24_I	263_I	0.728	1.00
173_I	200_T	0.725	1.00
91_F	95_L	0.722	1.00
187_L	196_E	0.716	1.00
29_Y	268_F	0.716	1.00
80_I	83_A	0.711	1.00
265_V	269_M	0.705	1.00
180_E	184_V	0.705	1.00
53_I	64_L	0.702	1.00
93_A	216_A	0.701	1.00
259_A	262_T	0.698	1.00
260_F	264_V	0.697	1.00
61_N	65_I	0.695	1.00
32_I	80_I	0.694	1.00
8_E	281_E	0.692	1.00
242_Q	246_Y	0.687	1.00
17_K	21_V	0.679	1.00
23_L	275_L	0.678	1.00
42_K	63_Y	0.677	1.00
27_V	31_L	0.675	1.00
127_L	160_A	0.672	1.00
13_L	17_K	0.670	1.00
69_G	254_A	0.669	1.00
169_Q	204_Y	0.669	1.00
53_I	57_P	0.666	1.00
65_I	76_L	0.665	1.00
192_I	197_V	0.665	1.00
281_E	287_I	0.664	1.00
19_S	23_L	0.661	1.00
267_G	271_G	0.660	1.00
241_Q	244_L	0.659	1.00
29_Y	33_A	0.654	1.00
210_I	272_L	0.652	1.00
40_F	44_Y	0.650	1.00
218_Y	221_V	0.650	1.00
280_D	287_I	0.649	1.00
100_I	155_S	0.646	1.00
275_L	279_I	0.646	1.00
166_V	169_Q	0.646	1.00
169_Q	201_L	0.643	1.00
32_I	255_I	0.642	1.00
158_S	161_G	0.641	1.00
20_R	270_S	0.639	1.00
79_I	261_P	0.637	1.00
96_F	151_S	0.635	1.00
269_M	273_I	0.634	1.00
112_G	116_D	0.632	1.00
190_K	193_I	0.631	1.00
120_M	123_T	0.629	1.00
32_I	58_Q	0.628	1.00
150_L	220_E	0.628	1.00
247_G	258_L	0.628	1.00
64_L	68_T	0.626	1.00
97_A	155_S	0.623	1.00
61_N	64_L	0.623	1.00
101_G	105_G	0.622	1.00
118_I	121_G	0.622	1.00
27_V	262_T	0.621	1.00
220_E	224_Y	0.617	1.00
9_Y	278_G	0.617	1.00
29_Y	32_I	0.616	1.00
13_L	274_L	0.615	1.00
144_T	148_I	0.614	1.00
18_K	22_G	0.613	1.00
157_T	160_A	0.611	1.00
258_L	262_T	0.609	1.00
36_G	247_G	0.605	1.00
108_A	120_M	0.604	1.00
52_T	64_L	0.596	1.00
49_S	55_M	0.589	1.00
40_F	81_Q	0.588	1.00
87_I	91_F	0.587	1.00
13_L	19_S	0.586	1.00
131_S	134_L	0.585	1.00
123_T	159_W	0.582	1.00
100_I	104_V	0.582	1.00
219_A	222_G	0.580	1.00
171_L	174_R	0.580	1.00
112_G	171_L	0.578	1.00
218_Y	222_G	0.577	1.00
161_G	212_N	0.575	1.00
119_L	123_T	0.574	1.00
137_I	141_A	0.573	1.00
149_F	153_I	0.573	1.00
94_G	213_V	0.573	1.00
83_A	87_I	0.572	1.00
20_R	24_I	0.571	1.00
110_Y	194_F	0.571	1.00
70_P	246_Y	0.567	1.00
41_P	76_L	0.561	1.00
101_G	163_A	0.560	1.00
162_L	208_H	0.560	1.00
70_P	256_Y	0.559	1.00
217_V	221_V	0.558	1.00
110_Y	174_R	0.557	1.00
42_K	64_L	0.556	1.00
111_L	193_I	0.555	1.00
162_L	166_V	0.555	1.00
240_I	265_V	0.553	1.00
94_G	212_N	0.552	1.00
277_Y	280_D	0.550	1.00
158_S	212_N	0.550	1.00
134_L	157_T	0.549	1.00
90_S	216_A	0.549	1.00
126_I	159_W	0.548	1.00
25_I	58_Q	0.544	1.00
156_I	159_W	0.543	1.00
131_S	156_I	0.541	1.00
8_E	287_I	0.539	1.00
109_G	116_D	0.537	1.00
187_L	190_K	0.537	1.00
179_V	183_R	0.536	1.00
216_A	219_A	0.536	1.00
139_V	143_K	0.533	1.00
25_I	267_G	0.532	1.00
89_I	150_L	0.531	1.00
221_V	241_Q	0.530	1.00
16_N	278_G	0.529	1.00
98_T	205_I	0.527	1.00
29_Y	58_Q	0.523	1.00
11_R	15_D	0.523	1.00
207_I	210_I	0.523	1.00
41_P	77_V	0.522	1.00
25_I	59_L	0.517	1.00
194_F	199_P	0.516	1.00
50_P	57_P	0.516	1.00
29_Y	267_G	0.514	1.00
161_G	208_H	0.513	1.00
152_L	156_I	0.511	1.00
165_A	169_Q	0.510	1.00
95_L	99_L	0.508	1.00
108_A	167_R	0.507	1.00
84_K	87_I	0.506	1.00
244_L	247_G	0.505	1.00
221_V	238_A	0.503	1.00
120_M	124_D	0.503	1.00
26_T	272_L	0.500	1.00
121_G	125_I	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4tquM	1	0.8724	99.9	0.6	Contact Map
3tuiA	2	0.7	99.9	0.626	Contact Map
3d31C	2	0.8	99.9	0.636	Contact Map
2onkC	2	0.8172	99.8	0.652	Contact Map
3rlfF	1	0.8931	99.8	0.658	Contact Map
4ymuD	2	0.7207	99.8	0.661	Contact Map
4tquN	1	0.9103	99.8	0.673	Contact Map
3rlfG	1	0.8862	99.8	0.677	Contact Map
3j9pD	4	0.7724	44.1	0.941	Contact Map
3j5pB	3	0.8241	5.3	0.962	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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