Q97VC7 - SULSO - GREMLIN Contacts

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Q97VC7 - Regucalcin homolog

UniProt:	Q97VC7	Sequences: MEPNLITLSNYKATLGEGPVYDKELNKLFWVDIEGKRIIVNDLNTGTEVFYEMPDLISSLCVIDDKRVIATIRHGFYIVNLDRGEIQKIAEVETDMESNRFNDGKCDKLGRYWAGTMNMNERKPTGSFYKLDTGKITKILEGLTVSNGLGWDPDNTQMFLIDSPVRKVFVFDFDLNKGEIYNRRVAVDFGNEPGNPDGMAVDEEGYIWVAHWGGGKVSRWNPKTGKKLFEIKVPATYVTSVTFGTQELDQLFITTAGKSQDPLAGKTFTTKANVRGLQNFRFKIS	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	285 (226)
Sequences:	11290 (9327)
Seq/√Len:	620.4

ECOLI - YNCE TOLB 6PGL BAMB
BACSU - YVRE YWHL 6PGL YXAL YWHK
HALSA - Q9HHP3 Q9HS46
SULSO - Q97VC7 Q97UH7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
103_D	116_T	3.410	1.00
142_G	161_I	3.306	1.00
115_G	129_Y	3.091	1.00
148_G	162_D	3.087	1.00
198_G	211_H	2.886	1.00
234_P	253_I	2.833	1.00
192_E	210_A	2.823	1.00
210_A	220_W	2.704	1.00
244_G	247_E	2.585	1.00
161_I	171_F	2.531	1.00
31_V	41_N	2.502	1.00
30_W	40_V	2.490	1.00
114_A	151_W	2.461	1.00
160_L	201_V	2.257	1.00
240_S	254_T	2.217	1.00
209_V	243_F	2.153	1.00
117_M	123_K	2.118	1.00
22_D	25_L	2.091	1.00
126_G	161_I	2.081	1.00
221_N	224_T	2.064	1.00
30_W	62_V	2.029	1.00
209_V	219_R	2.010	1.00
114_A	159_F	1.991	1.00
130_K	133_T	1.987	1.00
53_M	71_T	1.971	1.00
114_A	128_F	1.970	1.00
93_E	115_G	1.937	1.00
105_K	150_G	1.921	1.00
80_N	83_R	1.898	1.00
160_L	208_W	1.897	1.00
142_G	163_S	1.860	1.00
77_Y	86_I	1.845	1.00
18_G	32_D	1.812	1.00
218_S	230_E	1.801	1.00
160_L	170_V	1.758	1.00
162_D	196_P	1.753	1.00
218_S	227_K	1.740	1.00
76_F	115_G	1.724	1.00
217_V	253_I	1.715	1.00
63_I	106_C	1.705	1.00
42_D	45_T	1.692	1.00
168_V	210_A	1.680	1.00
116_T	146_S	1.655	1.00
255_T	258_K	1.630	1.00
107_D	151_W	1.629	1.00
220_W	227_K	1.608	1.00
127_S	138_K	1.566	1.00
102_N	118_N	1.538	1.00
107_D	111_R	1.521	1.00
106_C	113_W	1.428	1.00
61_C	105_K	1.390	1.00
153_P	201_V	1.370	1.00
244_G	248_L	1.359	1.00
105_K	149_L	1.354	1.00
209_V	251_L	1.352	1.00
71_T	79_V	1.343	1.00
70_A	106_C	1.331	1.00
151_W	159_F	1.327	1.00
211_H	238_V	1.308	1.00
33_I	36_K	1.299	1.00
160_L	199_M	1.290	1.00
77_Y	89_I	1.274	1.00
33_I	37_R	1.271	1.00
149_L	161_I	1.269	1.00
210_A	218_S	1.267	1.00
70_A	78_I	1.245	1.00
227_K	230_E	1.237	1.00
64_D	110_G	1.233	1.00
104_G	115_G	1.233	1.00
115_G	127_S	1.224	1.00
234_P	255_T	1.220	1.00
202_D	205_G	1.217	1.00
209_V	241_V	1.208	1.00
151_W	173_F	1.203	1.00
203_E	248_L	1.192	1.00
152_D	201_V	1.182	1.00
127_S	135_K	1.174	1.00
111_R	132_D	1.168	1.00
142_G	165_V	1.165	1.00
20_V	61_C	1.165	1.00
200_A	242_T	1.157	1.00
108_K	151_W	1.142	1.00
216_K	230_E	1.139	1.00
202_D	206_Y	1.136	1.00
31_V	39_I	1.131	1.00
123_K	127_S	1.125	1.00
35_G	39_I	1.120	1.00
33_I	39_I	1.118	1.00
116_T	149_L	1.113	1.00
32_D	36_K	1.109	1.00
68_V	71_T	1.097	1.00
79_V	86_I	1.096	1.00
163_S	166_R	1.095	1.00
192_E	212_W	1.094	1.00
211_H	215_G	1.091	1.00
152_D	156_T	1.091	1.00
117_M	125_T	1.089	1.00
169_F	188_D	1.089	1.00
243_F	251_L	1.088	1.00
61_C	104_G	1.088	1.00
115_G	123_K	1.087	1.00
200_A	241_V	1.081	1.00
147_N	164_P	1.063	1.00
192_E	196_P	1.057	1.00
185_V	188_D	1.056	1.00
32_D	57_I	1.055	1.00
153_P	205_G	1.053	1.00
108_K	155_N	1.052	1.00
197_D	213_G	1.052	1.00
212_W	216_K	1.045	1.00
64_D	106_C	1.045	1.00
244_G	249_D	1.042	1.00
162_D	199_M	1.038	1.00
117_M	124_P	1.025	1.00
150_G	200_A	1.022	1.00
244_G	252_F	1.021	1.00
163_S	167_K	1.015	1.00
211_H	241_V	1.011	1.00
19_P	31_V	1.008	1.00
86_I	89_I	1.003	1.00
168_V	199_M	0.994	1.00
142_G	146_S	0.992	1.00
200_A	243_F	0.991	1.00
141_E	171_F	0.989	1.00
241_V	253_I	0.986	1.00
114_A	149_L	0.986	1.00
244_G	250_Q	0.985	1.00
202_D	243_F	0.984	1.00
70_A	113_W	0.984	1.00
20_V	60_L	0.983	1.00
39_I	48_E	0.972	1.00
170_V	201_V	0.949	1.00
76_F	104_G	0.944	1.00
53_M	57_I	0.936	1.00
31_V	35_G	0.934	1.00
21_Y	29_F	0.933	1.00
41_N	48_E	0.932	1.00
150_G	199_M	0.930	1.00
67_R	80_N	0.927	1.00
165_V	169_F	0.925	1.00
234_P	238_V	0.920	1.00
234_P	257_G	0.918	1.00
106_C	131_L	0.911	1.00
61_C	106_C	0.911	1.00
203_E	243_F	0.910	1.00
202_D	207_I	0.908	1.00
199_M	210_A	0.901	1.00
30_W	69_I	0.898	1.00
212_W	218_S	0.897	1.00
146_S	163_S	0.895	1.00
63_I	67_R	0.895	1.00
145_V	164_P	0.891	1.00
73_R	77_Y	0.880	1.00
129_Y	135_K	0.879	1.00
172_D	182_N	0.877	1.00
192_E	214_G	0.877	1.00
161_I	169_F	0.876	1.00
27_K	42_D	0.873	1.00
216_K	232_K	0.869	1.00
22_D	62_V	0.865	1.00
21_Y	106_C	0.862	1.00
30_W	60_L	0.859	1.00
214_G	218_S	0.856	1.00
135_K	138_K	0.850	1.00
102_N	147_N	0.849	1.00
53_M	79_V	0.848	1.00
254_T	258_K	0.845	1.00
142_G	169_F	0.845	1.00
195_N	213_G	0.844	1.00
233_V	253_I	0.844	1.00
241_V	251_L	0.843	1.00
208_W	222_P	0.837	1.00
212_W	215_G	0.831	1.00
99_N	118_N	0.824	1.00
152_D	155_N	0.823	1.00
207_I	219_R	0.822	1.00
107_D	112_Y	0.820	1.00
63_I	66_K	0.817	1.00
206_Y	221_N	0.815	1.00
59_S	104_G	0.814	1.00
147_N	197_D	0.812	1.00
25_L	28_L	0.812	1.00
62_V	81_L	0.803	1.00
242_T	252_F	0.794	1.00
202_D	209_V	0.790	1.00
213_G	237_Y	0.788	1.00
23_K	62_V	0.786	1.00
38_I	71_T	0.786	1.00
70_A	104_G	0.784	1.00
118_N	124_P	0.773	1.00
239_T	256_A	0.773	1.00
93_E	117_M	0.773	1.00
117_M	127_S	0.770	1.00
200_A	209_V	0.770	1.00
191_N	220_W	0.767	1.00
105_K	151_W	0.760	1.00
156_T	174_D	0.758	1.00
198_G	241_V	0.756	1.00
152_D	158_M	0.750	1.00
23_K	66_K	0.748	1.00
32_D	35_G	0.744	1.00
191_N	218_S	0.737	1.00
101_F	117_M	0.734	1.00
22_D	30_W	0.734	1.00
22_D	27_K	0.732	1.00
103_D	149_L	0.732	1.00
105_K	114_A	0.730	1.00
38_I	60_L	0.725	1.00
212_W	220_W	0.718	1.00
169_F	185_V	0.715	1.00
80_N	87_Q	0.715	1.00
30_W	38_I	0.710	1.00
21_Y	43_L	0.698	1.00
101_F	115_G	0.698	1.00
126_G	149_L	0.697	1.00
207_I	228_L	0.695	1.00
101_F	104_G	0.693	1.00
104_G	113_W	0.693	1.00
185_V	189_F	0.692	1.00
42_D	46_G	0.691	1.00
192_E	218_S	0.691	1.00
20_V	30_W	0.686	1.00
70_A	79_V	0.684	1.00
201_V	208_W	0.675	1.00
106_C	151_W	0.674	1.00
163_S	169_F	0.673	1.00
40_V	62_V	0.669	1.00
29_F	43_L	0.668	1.00
247_E	250_Q	0.666	1.00
107_D	110_G	0.665	1.00
172_D	184_R	0.663	1.00
92_V	115_G	0.663	1.00
201_V	222_P	0.660	1.00
151_W	201_V	0.657	1.00
22_D	66_K	0.652	1.00
107_D	114_A	0.650	1.00
113_W	131_L	0.650	1.00
28_L	40_V	0.647	1.00
59_S	103_D	0.647	1.00
112_Y	128_F	0.644	1.00
42_D	47_T	0.644	1.00
22_D	26_N	0.644	1.00
78_I	90_A	0.642	1.00
163_S	171_F	0.642	1.00
154_D	205_G	0.638	1.00
204_E	207_I	0.634	1.00
53_M	77_Y	0.633	1.00
27_K	44_N	0.632	1.00
149_L	159_F	0.632	1.00
20_V	62_V	0.625	1.00
196_P	199_M	0.625	1.00
171_F	185_V	0.624	1.00
183_R	186_A	0.621	1.00
221_N	228_L	0.619	1.00
142_G	171_F	0.619	1.00
43_L	46_G	0.618	1.00
16_G	41_N	0.615	1.00
183_R	187_V	0.614	1.00
215_G	237_Y	0.613	1.00
63_I	69_I	0.612	1.00
206_Y	223_K	0.612	1.00
162_D	166_R	0.609	1.00
182_N	185_V	0.606	1.00
99_N	117_M	0.606	1.00
125_T	138_K	0.605	1.00
196_P	210_A	0.602	1.00
58_S	72_I	0.599	1.00
150_G	201_V	0.599	1.00
192_E	220_W	0.596	1.00
186_A	189_F	0.596	1.00
57_I	60_L	0.593	1.00
78_I	106_C	0.591	1.00
152_D	160_L	0.586	1.00
60_L	69_I	0.586	1.00
130_K	134_G	0.584	1.00
18_G	60_L	0.582	1.00
28_L	32_D	0.580	1.00
146_S	161_I	0.578	1.00
160_L	168_V	0.578	1.00
148_G	196_P	0.575	1.00
126_G	159_F	0.575	1.00
111_R	130_K	0.575	1.00
112_Y	116_T	0.575	1.00
67_R	82_D	0.574	1.00
238_V	253_I	0.571	1.00
114_A	126_G	0.571	1.00
196_P	214_G	0.566	1.00
253_I	257_G	0.565	1.00
238_V	241_V	0.563	1.00
58_S	102_N	0.561	1.00
116_T	126_G	0.561	1.00
168_V	196_P	0.560	1.00
62_V	69_I	0.557	1.00
118_N	145_V	0.556	1.00
184_R	187_V	0.555	1.00
53_M	73_R	0.555	1.00
166_R	195_N	0.554	1.00
81_L	84_G	0.551	1.00
100_R	118_N	0.551	1.00
222_P	225_G	0.551	1.00
63_I	68_V	0.546	0.99
16_G	33_I	0.546	0.99
102_N	145_V	0.545	0.99
55_D	73_R	0.544	0.99
16_G	31_V	0.544	0.99
30_W	68_V	0.543	0.99
150_G	160_L	0.541	0.99
246_Q	249_D	0.539	0.99
141_E	169_F	0.539	0.99
52_E	77_Y	0.539	0.99
217_V	238_V	0.537	0.99
36_K	56_L	0.536	0.99
92_V	129_Y	0.535	0.99
60_L	71_T	0.534	0.99
221_N	226_K	0.534	0.99
72_I	75_G	0.534	0.99
63_I	110_G	0.531	0.99
154_D	158_M	0.531	0.99
112_Y	151_W	0.530	0.99
92_V	127_S	0.529	0.99
126_G	139_I	0.524	0.99
128_F	151_W	0.524	0.99
128_F	137_T	0.522	0.99
32_D	60_L	0.522	0.99
167_K	188_D	0.521	0.99
52_E	79_V	0.518	0.99
62_V	106_C	0.516	0.99
55_D	72_I	0.514	0.99
80_N	84_G	0.514	0.99
112_Y	136_I	0.514	0.99
103_D	148_G	0.511	0.99
76_F	113_W	0.509	0.99
16_G	35_G	0.507	0.99
197_D	239_T	0.507	0.99
239_T	242_T	0.506	0.99
144_T	164_P	0.503	0.99
16_G	19_P	0.503	0.99
93_E	129_Y	0.501	0.99
57_I	71_T	0.500	0.99

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1vyhC	1	0.8632	100	0.195	Contact Map
4wjuA	1	0.8632	100	0.218	Contact Map
4j73A	1	0.9228	100	0.22	Contact Map
4u1eI	1	0.9123	100	0.221	Contact Map
1gotB	1	0.8632	100	0.225	Contact Map
3sfzA	1	0.9404	100	0.225	Contact Map
3ow8A	3	0.8702	100	0.226	Contact Map
3dm0A	1	0.9123	100	0.23	Contact Map
3j60g	1	0.9053	100	0.237	Contact Map
5a1uD	1	0.9088	100	0.239	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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