Q97VC4 - SULSO - GREMLIN Contacts

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Q97VC4 - Uncharacterized protein

UniProt:	Q97VC4	Sequences: MSEILELINRGKYNEALKRIKEEIEQKKDDPELYYLMGLVYFKIGKYRRAIDNLRKAIRLKKDEAKYHCLLGYSLYMEGYNISDEKLIRRALKELQLSLEMDKDLPNKEKKYYYYMANILYELEDYEKALEKVNKALEIDDSSEVHKLRGDILFQLKKYNEAIEEYKTNLNDDKNLYAIAYTYFTMGKYDQALEYFNKAISANPEDPYYYQGKAETLLFMGRTNEAYNTIKRALEIDPDNPYIKLTEVEILTETNEKEAIKVLTEYLEEMEEFREIVCEEIREGKFKEKTKEVIEKVITKFC	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	302 (246)
Sequences:	32155 (28355)
Seq/√Len:	1807.8

ECOLI - LPSRP HEMY NLPI YBEQ PGAA YPHG BCSC BAMD
BACSU - YVCD YPIA RAPE RAPB RAPA RAPH RAPJ RAPG RAPI RAPK RAPC RAPF YRRB RAPD
HALSA - Q9HMM8
SULSO - Q97VC4 Q97V36 Q97WM3 Q97ZS3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
222_R	225_E	3.499	1.00
188_K	191_Q	3.357	1.00
125_D	128_K	3.313	1.00
87_L	90_R	3.230	1.00
158_K	161_E	3.226	1.00
46_K	49_R	3.217	1.00
210_Y	229_T	2.914	1.00
146_H	165_E	2.629	1.00
34_Y	53_N	2.608	1.00
214_A	230_I	2.445	1.00
150_G	166_Y	2.376	1.00
117_A	133_V	2.364	1.00
113_Y	132_K	2.342	1.00
176_L	195_Y	2.323	1.00
180_A	196_F	2.264	1.00
197_N	213_K	2.166	1.00
213_K	229_T	2.148	1.00
38_G	54_L	2.134	1.00
244_K	262_V	2.095	1.00
217_L	229_T	2.022	1.00
153_L	165_E	2.020	1.00
134_N	149_R	2.017	1.00
120_L	132_K	2.013	1.00
116_M	132_K	2.010	1.00
21_K	37_M	2.003	1.00
179_I	195_Y	1.998	1.00
37_M	53_N	1.978	1.00
149_R	165_E	1.914	1.00
217_L	225_E	1.909	1.00
20_I	36_L	1.887	1.00
231_K	247_E	1.803	1.00
68_H	94_E	1.794	1.00
133_V	148_L	1.779	1.00
41_Y	49_R	1.777	1.00
167_K	179_I	1.767	1.00
41_Y	53_N	1.764	1.00
96_Q	116_M	1.748	1.00
193_L	197_N	1.744	1.00
153_L	161_E	1.723	1.00
183_Y	195_Y	1.721	1.00
120_L	128_K	1.720	1.00
183_Y	191_Q	1.708	1.00
92_L	96_Q	1.678	1.00
248_V	263_L	1.658	1.00
95_L	115_Y	1.648	1.00
227_Y	231_K	1.648	1.00
196_F	212_G	1.613	1.00
163_I	167_K	1.605	1.00
166_Y	178_A	1.508	1.00
130_L	153_L	1.503	1.00
55_R	71_L	1.494	1.00
120_L	125_D	1.478	1.00
130_L	134_N	1.468	1.00
230_I	246_T	1.466	1.00
51_I	55_R	1.436	1.00
17_L	21_K	1.410	1.00
193_L	220_M	1.393	1.00
251_L	262_V	1.388	1.00
113_Y	135_K	1.386	1.00
183_Y	188_K	1.370	1.00
37_M	41_Y	1.362	1.00
193_L	217_L	1.356	1.00
92_L	120_L	1.346	1.00
213_K	217_L	1.342	1.00
153_L	158_K	1.341	1.00
130_L	156_L	1.291	1.00
210_Y	232_R	1.273	1.00
136_A	145_V	1.268	1.00
17_L	41_Y	1.266	1.00
116_M	120_L	1.263	1.00
146_H	168_T	1.261	1.00
161_E	164_E	1.248	1.00
41_Y	46_K	1.239	1.00
227_Y	251_L	1.238	1.00
233_A	243_I	1.233	1.00
199_A	209_Y	1.218	1.00
179_I	183_Y	1.217	1.00
177_Y	212_G	1.215	1.00
247_E	262_V	1.209	1.00
247_E	251_L	1.190	1.00
215_E	246_T	1.187	1.00
217_L	222_R	1.159	1.00
251_L	258_E	1.158	1.00
54_L	70_L	1.154	1.00
72_G	95_L	1.148	1.00
98_S	112_Y	1.128	1.00
71_L	75_L	1.126	1.00
227_Y	254_T	1.123	1.00
218_L	246_T	1.122	1.00
163_I	186_M	1.120	1.00
137_L	146_H	1.117	1.00
176_L	198_K	1.110	1.00
181_Y	212_G	1.090	1.00
232_R	235_E	1.080	1.00
92_L	123_L	1.074	1.00
23_E	33_L	1.073	1.00
193_L	216_T	1.070	1.00
163_I	183_Y	1.059	1.00
117_A	136_A	1.059	1.00
17_L	44_I	1.058	1.00
149_R	153_L	1.057	1.00
71_L	94_E	1.057	1.00
130_L	149_R	1.053	1.00
163_I	179_I	1.051	1.00
251_L	256_E	1.049	1.00
35_Y	39_L	1.049	1.00
64_E	67_Y	1.049	1.00
118_N	148_L	1.044	1.00
211_Q	246_T	1.041	1.00
128_K	131_E	1.041	1.00
75_L	94_E	1.041	1.00
170_L	176_L	1.025	1.00
232_R	236_I	1.023	1.00
206_D	209_Y	1.023	1.00
200_I	210_Y	1.020	1.00
245_L	249_E	1.016	1.00
114_Y	145_V	1.012	1.00
99_L	113_Y	1.003	1.00
147_K	178_A	0.999	1.00
234_L	244_K	0.999	1.00
34_Y	56_K	0.998	1.00
92_L	116_M	0.997	1.00
260_I	264_T	0.988	1.00
56_K	60_L	0.986	1.00
130_L	152_I	0.978	1.00
184_F	216_T	0.978	1.00
193_L	213_K	0.977	1.00
214_A	233_A	0.976	1.00
165_E	168_T	0.975	1.00
191_Q	194_E	0.968	1.00
151_D	178_A	0.967	1.00
114_Y	148_L	0.965	1.00
237_D	240_N	0.962	1.00
215_E	219_F	0.954	1.00
211_Q	215_E	0.954	1.00
248_V	259_A	0.948	1.00
207_P	233_A	0.948	1.00
180_A	199_A	0.948	1.00
146_H	169_N	0.947	1.00
225_E	228_N	0.940	1.00
211_Q	243_I	0.939	1.00
132_K	135_K	0.936	1.00
69_C	72_G	0.934	1.00
30_D	33_L	0.934	1.00
135_K	139_I	0.934	1.00
150_G	162_A	0.933	1.00
218_L	230_I	0.933	1.00
203_N	206_D	0.933	1.00
143_S	169_N	0.931	1.00
184_F	212_G	0.930	1.00
159_Y	182_T	0.928	1.00
137_L	149_R	0.919	1.00
24_I	34_Y	0.916	1.00
229_T	232_R	0.912	1.00
244_K	265_E	0.903	1.00
117_A	129_A	0.902	1.00
245_L	248_V	0.900	1.00
127_E	156_L	0.897	1.00
38_G	50_A	0.895	1.00
126_Y	156_L	0.891	1.00
198_K	202_A	0.887	1.00
150_G	169_N	0.884	1.00
110_K	136_A	0.883	1.00
137_L	145_V	0.879	1.00
177_Y	181_Y	0.877	1.00
19_R	22_E	0.875	1.00
88_I	119_I	0.873	1.00
49_R	52_D	0.868	1.00
27_K	30_D	0.866	1.00
17_L	37_M	0.866	1.00
168_T	171_N	0.865	1.00
31_P	57_A	0.865	1.00
102_D	109_E	0.864	1.00
90_R	93_K	0.859	1.00
240_N	243_I	0.856	1.00
14_N	44_I	0.855	1.00
126_Y	152_I	0.854	1.00
227_Y	247_E	0.852	1.00
184_F	219_F	0.851	1.00
212_G	215_E	0.849	1.00
113_Y	136_A	0.842	1.00
207_P	237_D	0.836	1.00
176_L	199_A	0.835	1.00
40_V	44_I	0.832	1.00
51_I	75_L	0.831	1.00
73_Y	77_M	0.831	1.00
70_L	73_Y	0.830	1.00
89_R	123_L	0.828	1.00
163_I	182_T	0.824	1.00
51_I	78_E	0.820	1.00
98_S	101_M	0.818	1.00
113_Y	139_I	0.817	1.00
94_E	97_L	0.817	1.00
246_T	249_E	0.816	1.00
147_K	151_D	0.815	1.00
198_K	201_S	0.815	1.00
182_T	186_M	0.812	1.00
210_Y	233_A	0.810	1.00
195_Y	198_K	0.810	1.00
121_Y	148_L	0.810	1.00
163_I	188_K	0.809	1.00
224_N	254_T	0.806	1.00
252_T	259_A	0.805	1.00
152_I	156_L	0.805	1.00
75_L	90_R	0.802	1.00
56_K	59_R	0.801	1.00
218_L	253_E	0.800	1.00
189_Y	216_T	0.799	1.00
218_L	226_A	0.798	1.00
216_T	220_M	0.797	1.00
135_K	138_E	0.795	1.00
214_A	226_A	0.794	1.00
58_I	68_H	0.794	1.00
252_T	263_L	0.789	1.00
38_G	57_A	0.788	1.00
227_Y	250_I	0.785	1.00
200_I	209_Y	0.780	1.00
190_D	220_M	0.775	1.00
109_E	112_Y	0.773	1.00
160_N	186_M	0.770	1.00
36_L	39_L	0.769	1.00
223_T	253_E	0.769	1.00
177_Y	209_Y	0.767	1.00
39_L	70_L	0.763	1.00
180_A	192_A	0.763	1.00
154_F	182_T	0.762	1.00
258_E	261_K	0.760	1.00
74_S	78_E	0.760	1.00
15_E	18_K	0.750	1.00
115_Y	118_N	0.748	1.00
181_Y	184_F	0.748	1.00
97_L	101_M	0.745	1.00
121_Y	152_I	0.744	1.00
119_I	123_L	0.739	1.00
35_Y	67_Y	0.732	1.00
215_E	218_L	0.732	1.00
69_C	73_Y	0.728	1.00
114_Y	118_N	0.726	1.00
42_F	70_L	0.725	1.00
53_N	56_K	0.722	1.00
42_F	50_A	0.720	1.00
176_L	202_A	0.719	1.00
263_L	267_L	0.718	1.00
223_T	250_I	0.717	1.00
151_D	154_F	0.714	1.00
250_I	254_T	0.706	1.00
210_Y	236_I	0.705	1.00
121_Y	155_Q	0.703	1.00
130_L	158_K	0.702	1.00
148_L	151_D	0.698	1.00
199_A	202_A	0.697	1.00
159_Y	163_I	0.697	1.00
99_L	116_M	0.696	1.00
159_Y	185_T	0.695	1.00
223_T	254_T	0.695	1.00
39_L	43_K	0.694	1.00
17_L	40_V	0.693	1.00
181_Y	185_T	0.692	1.00
151_D	155_Q	0.690	1.00
61_K	64_E	0.688	1.00
99_L	112_Y	0.687	1.00
93_K	97_L	0.684	1.00
248_V	266_Y	0.682	1.00
51_I	74_S	0.679	1.00
16_A	20_I	0.673	1.00
261_K	265_E	0.673	1.00
57_A	67_Y	0.670	1.00
34_Y	57_A	0.669	1.00
189_Y	220_M	0.667	1.00
194_E	198_K	0.666	1.00
24_I	33_L	0.661	1.00
31_P	61_K	0.659	1.00
164_E	168_T	0.658	1.00
154_F	178_A	0.656	1.00
103_K	109_E	0.654	1.00
114_Y	136_A	0.651	1.00
154_F	162_A	0.650	1.00
126_Y	130_L	0.648	1.00
55_R	59_R	0.645	1.00
35_Y	70_L	0.643	1.00
92_L	119_I	0.643	1.00
225_E	229_T	0.639	1.00
126_Y	155_Q	0.637	1.00
97_L	100_E	0.634	1.00
13_Y	40_V	0.632	1.00
47_Y	51_I	0.631	1.00
189_Y	219_F	0.630	1.00
184_F	189_Y	0.624	1.00
178_A	181_Y	0.623	1.00
23_E	26_Q	0.623	1.00
131_E	135_K	0.622	1.00
211_Q	240_N	0.617	1.00
218_L	223_T	0.616	1.00
57_A	60_L	0.615	1.00
231_K	235_E	0.614	1.00
196_F	216_T	0.611	1.00
248_V	252_T	0.610	1.00
76_Y	115_Y	0.608	1.00
262_V	265_E	0.607	1.00
234_L	243_I	0.607	1.00
226_A	250_I	0.606	1.00
162_A	182_T	0.602	1.00
170_L	179_I	0.602	1.00
19_R	23_E	0.601	1.00
48_R	78_E	0.597	1.00
42_F	47_Y	0.596	1.00
189_Y	193_L	0.596	1.00
228_N	232_R	0.594	1.00
211_Q	233_A	0.593	1.00
154_F	185_T	0.591	1.00
260_I	263_L	0.590	1.00
193_L	222_R	0.590	1.00
234_L	247_E	0.589	1.00
68_H	97_L	0.587	1.00
42_F	54_L	0.585	1.00
34_Y	60_L	0.584	1.00
168_T	172_D	0.581	1.00
110_K	114_Y	0.581	1.00
230_I	250_I	0.579	1.00
91_A	95_L	0.578	1.00
118_N	121_Y	0.576	1.00
35_Y	64_E	0.574	1.00
75_L	87_L	0.574	1.00
90_R	94_E	0.568	1.00
184_F	196_F	0.566	1.00
78_E	87_L	0.562	1.00
154_F	159_Y	0.560	1.00
161_E	165_E	0.557	1.00
20_I	24_I	0.555	1.00
35_Y	57_A	0.554	1.00
24_I	37_M	0.554	1.00
121_Y	126_Y	0.553	1.00
128_K	132_K	0.552	1.00
159_Y	186_M	0.551	1.00
95_L	119_I	0.550	1.00
227_Y	256_E	0.549	1.00
197_N	217_L	0.549	1.00
264_T	267_L	0.546	1.00
18_K	22_E	0.546	1.00
49_R	53_N	0.542	1.00
118_N	122_E	0.541	1.00
167_K	171_N	0.539	1.00
132_K	136_A	0.537	1.00
218_L	250_I	0.532	1.00
262_V	266_Y	0.532	1.00
13_Y	43_K	0.532	1.00
233_A	236_I	0.531	1.00
20_I	37_M	0.531	1.00
244_K	266_Y	0.529	1.00
136_A	139_I	0.527	1.00
217_L	220_M	0.527	1.00
98_S	102_D	0.522	1.00
257_K	260_I	0.521	1.00
22_E	26_Q	0.521	1.00
134_N	137_L	0.517	1.00
22_E	25_E	0.516	1.00
207_P	211_Q	0.516	1.00
92_L	125_D	0.516	1.00
13_Y	44_I	0.515	1.00
96_Q	100_E	0.515	1.00
169_N	172_D	0.511	1.00
151_D	166_Y	0.509	1.00
20_I	23_E	0.509	1.00
200_I	213_K	0.507	1.00
191_Q	195_Y	0.506	1.00
121_Y	129_A	0.504	1.00
134_N	153_L	0.504	1.00
224_N	227_Y	0.501	1.00
51_I	71_L	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1w3bA	2	0.9636	100	0.209	Contact Map
2xpiA	2	0.9073	100	0.236	Contact Map
4ui9J	2	0.9338	100	0.244	Contact Map
4ui9C	2	0.9106	100	0.246	Contact Map
4l9pA	1	0.8113	100	0.246	Contact Map
3dssA	1	0.8377	100	0.247	Contact Map
4rg9A	2	0.7252	100	0.252	Contact Map
4eqfA	1	0.8278	100	0.257	Contact Map
4kvmA	3	0.9636	100	0.261	Contact Map
1fchA	3	0.8808	100	0.261	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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