Q97VB2 - SULSO - GREMLIN Contacts

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Q97VB2 - Second ORF in transposon ISC1229

UniProt:	Q97VB2	Sequences: MLWKEKDVRGKKTDFYDAEKLENMVNKAREYVNNPLKELVSLYLFMNDVQTKQENRLRRALFLVSDEEKLSEERLEKFSKGDFSDVKLYQLEYTGTVLREIQVLARTLLQTREELKEVRKMIEGNVPQDHVLLTIPGIGKLAAGIIIGIVGDIKRFPRPESFVAYCGLDPVVERSGRIEVRKGISKRGNKYLGSLFYFLAETQYSRNPTLLKFHEAHKDKLKGRKLYTALARKLARIVWSVWYNNKPYEVT	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	251 (236)
Sequences:	3962 (2920)
Seq/√Len:	190.1

SULSO - Q97VB2 Q97Y06 Q97YE1 Q97UF7 Q97X29 Q97WZ5 Q97X12 Q97ZN4 Q97X26 Q97Y59 Q97X07 Q97WC0 Q97UQ6 Q97XK2 Q97VS0 Q97Z62 Q97XR9 Q97XV5 Q97WY7 Q7LXX5 Q97XM7 Q97UU8 Q97TX3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
99_R	121_M	3.280	1.00
50_Q	112_R	2.996	1.00
217_H	220_K	2.958	1.00
103_V	114_E	2.763	1.00
166_C	192_L	2.668	1.00
150_V	162_F	2.641	1.00
162_F	235_A	2.572	1.00
134_T	247_P	2.488	1.00
106_R	114_E	2.335	1.00
49_V	52_K	2.320	1.00
157_P	161_S	2.317	1.00
49_V	56_R	2.251	1.00
57_L	108_L	2.135	1.00
211_L	215_E	2.098	1.00
158_R	161_S	2.080	1.00
131_V	247_P	2.070	1.00
110_Q	114_E	2.061	1.00
43_Y	119_R	2.037	1.00
146_I	238_V	2.037	1.00
49_V	107_T	1.967	1.00
41_S	149_I	1.939	1.00
47_N	115_L	1.934	1.00
184_I	228_T	1.927	1.00
126_V	133_L	1.916	1.00
138_I	143_A	1.916	1.00
222_K	225_K	1.891	1.00
232_R	236_R	1.888	1.00
50_Q	115_L	1.874	1.00
168_L	231_A	1.869	1.00
121_M	124_G	1.832	1.00
107_T	114_E	1.813	1.00
132_L	238_V	1.801	1.00
44_L	194_S	1.767	1.00
13_T	172_V	1.735	1.00
174_R	179_E	1.734	1.00
51_T	55_N	1.709	1.00
105_A	109_L	1.700	1.00
131_V	134_T	1.681	1.00
142_A	195_L	1.677	1.00
194_S	198_F	1.671	1.00
23_N	27_K	1.661	1.00
78_F	82_D	1.642	1.00
239_W	243_Y	1.638	1.00
241_V	247_P	1.616	1.00
116_K	119_R	1.615	1.00
156_F	165_Y	1.614	1.00
198_F	201_E	1.605	1.00
166_C	235_A	1.589	1.00
135_I	241_V	1.581	1.00
240_S	244_N	1.579	1.00
43_Y	47_N	1.574	1.00
106_R	113_E	1.569	1.00
46_M	115_L	1.563	1.00
107_T	110_Q	1.559	1.00
221_L	225_K	1.554	1.00
145_I	195_L	1.541	1.00
216_A	220_K	1.535	1.00
75_L	78_F	1.521	1.00
132_L	143_A	1.504	1.00
135_I	238_V	1.501	1.00
103_V	117_E	1.470	1.00
57_L	101_I	1.468	1.00
214_H	230_L	1.456	1.00
82_D	85_D	1.450	1.00
47_N	50_Q	1.433	1.00
200_A	234_L	1.431	1.00
204_Y	214_H	1.418	1.00
78_F	81_G	1.409	1.00
199_L	234_L	1.406	1.00
54_E	72_E	1.399	1.00
50_Q	108_L	1.390	1.00
163_V	232_R	1.374	1.00
200_A	231_A	1.343	1.00
106_R	110_Q	1.326	1.00
70_L	74_R	1.314	1.00
119_R	123_E	1.313	1.00
175_S	179_E	1.293	1.00
197_Y	231_A	1.286	1.00
131_V	241_V	1.283	1.00
133_L	140_K	1.275	1.00
45_F	56_R	1.273	1.00
153_I	242_W	1.263	1.00
72_E	76_E	1.262	1.00
110_Q	113_E	1.259	1.00
218_K	223_G	1.242	1.00
108_L	112_R	1.240	1.00
149_I	189_N	1.233	1.00
53_Q	57_L	1.230	1.00
142_A	199_L	1.225	1.00
40_V	191_Y	1.223	1.00
48_D	52_K	1.223	1.00
42_L	46_M	1.221	1.00
213_F	233_K	1.211	1.00
218_K	224_R	1.210	1.00
240_S	243_Y	1.204	1.00
80_K	83_F	1.203	1.00
210_L	230_L	1.199	1.00
74_R	77_K	1.196	1.00
123_E	140_K	1.188	1.00
78_F	83_F	1.185	1.00
19_E	23_N	1.172	1.00
210_L	237_I	1.161	1.00
73_E	77_K	1.138	1.00
135_I	234_L	1.138	1.00
49_V	53_Q	1.125	1.00
213_F	217_H	1.119	1.00
71_S	74_R	1.106	1.00
241_V	246_K	1.101	1.00
217_H	222_K	1.100	1.00
163_V	169_D	1.100	1.00
43_Y	115_L	1.094	1.00
79_S	82_D	1.087	1.00
40_V	122_I	1.068	1.00
95_G	121_M	1.066	1.00
44_L	191_Y	1.064	1.00
197_Y	228_T	1.060	1.00
132_L	242_W	1.059	1.00
75_L	82_D	1.052	1.00
224_R	227_Y	1.050	1.00
35_P	94_T	1.046	1.00
43_Y	141_L	1.040	1.00
213_F	230_L	1.038	1.00
112_R	116_K	1.031	1.00
213_F	229_A	1.030	1.00
50_Q	54_E	1.027	1.00
196_F	235_A	1.027	1.00
11_K	172_V	1.022	1.00
75_L	83_F	1.018	1.00
79_S	83_F	1.015	1.00
80_K	84_S	1.007	1.00
121_M	125_N	1.003	1.00
200_A	230_L	1.003	1.00
54_E	108_L	0.998	0.99
129_D	147_I	0.994	0.99
24_M	28_A	0.978	0.99
146_I	192_L	0.978	0.99
217_H	226_L	0.976	0.99
217_H	221_L	0.972	0.99
47_N	51_T	0.971	0.99
201_E	204_Y	0.971	0.99
103_V	121_M	0.970	0.99
186_K	193_G	0.969	0.99
184_I	193_G	0.969	0.99
44_L	48_D	0.967	0.99
201_E	224_R	0.966	0.99
98_L	102_Q	0.962	0.99
96_T	121_M	0.958	0.99
158_R	239_W	0.953	0.99
237_I	241_V	0.946	0.99
208_P	212_K	0.933	0.99
124_G	128_Q	0.933	0.99
138_I	238_V	0.925	0.99
79_S	85_D	0.923	0.99
53_Q	104_L	0.921	0.99
215_E	219_D	0.920	0.99
208_P	211_L	0.917	0.99
37_K	149_I	0.914	0.99
53_Q	107_T	0.913	0.99
210_L	234_L	0.906	0.99
12_K	17_D	0.900	0.99
40_V	144_G	0.887	0.99
68_E	78_F	0.876	0.99
39_L	118_V	0.872	0.99
99_R	103_V	0.871	0.99
53_Q	108_L	0.870	0.99
200_A	227_Y	0.870	0.99
126_V	144_G	0.869	0.99
201_E	227_Y	0.867	0.99
135_I	138_I	0.850	0.98
138_I	142_A	0.848	0.98
161_S	164_A	0.846	0.98
138_I	234_L	0.843	0.98
75_L	81_G	0.835	0.98
14_D	175_S	0.831	0.98
196_F	231_A	0.825	0.98
222_K	226_L	0.824	0.98
46_M	118_V	0.823	0.98
150_V	153_I	0.820	0.98
197_Y	201_E	0.815	0.98
202_T	206_R	0.814	0.98
169_D	185_S	0.810	0.98
52_K	55_N	0.807	0.98
76_E	80_K	0.805	0.98
99_R	102_Q	0.797	0.98
114_E	117_E	0.791	0.97
164_A	169_D	0.788	0.97
212_K	216_A	0.787	0.97
22_E	26_N	0.787	0.97
109_L	113_E	0.786	0.97
40_V	145_I	0.782	0.97
149_I	191_Y	0.779	0.97
213_F	216_A	0.768	0.97
42_L	118_V	0.767	0.97
117_E	121_M	0.765	0.97
184_I	229_A	0.763	0.97
75_L	79_S	0.763	0.97
172_V	179_E	0.762	0.97
68_E	82_D	0.762	0.97
123_E	133_L	0.760	0.97
21_L	24_M	0.759	0.97
152_D	155_R	0.755	0.97
216_A	219_D	0.740	0.96
77_K	81_G	0.740	0.96
70_L	73_E	0.736	0.96
68_E	84_S	0.733	0.96
68_E	74_R	0.730	0.96
70_L	81_G	0.729	0.96
16_Y	20_K	0.727	0.96
38_E	100_E	0.722	0.96
113_E	117_E	0.717	0.95
32_V	128_Q	0.717	0.95
77_K	80_K	0.711	0.95
46_M	114_E	0.708	0.95
201_E	231_A	0.707	0.95
62_F	67_E	0.706	0.95
70_L	79_S	0.705	0.95
30_E	33_N	0.705	0.95
159_P	239_W	0.705	0.95
75_L	80_K	0.698	0.95
200_A	203_Q	0.697	0.95
34_N	38_E	0.695	0.95
125_N	128_Q	0.694	0.95
55_N	59_R	0.690	0.94
81_G	85_D	0.686	0.94
59_R	63_L	0.684	0.94
115_L	119_R	0.683	0.94
47_N	119_R	0.682	0.94
109_L	112_R	0.681	0.94
96_T	99_R	0.680	0.94
107_T	111_T	0.679	0.94
100_E	103_V	0.675	0.94
42_L	100_E	0.673	0.94
169_D	172_V	0.665	0.93
188_G	193_G	0.664	0.93
217_H	229_A	0.659	0.93
8_V	11_K	0.658	0.93
71_S	75_L	0.653	0.93
69_K	78_F	0.653	0.93
212_K	215_E	0.650	0.92
71_S	78_F	0.648	0.92
119_R	141_L	0.647	0.92
70_L	82_D	0.645	0.92
41_S	191_Y	0.644	0.92
11_K	16_Y	0.641	0.92
45_F	49_V	0.639	0.92
236_R	239_W	0.635	0.91
163_V	236_R	0.633	0.91
41_S	189_N	0.632	0.91
145_I	149_I	0.630	0.91
69_K	72_E	0.628	0.91
78_F	85_D	0.628	0.91
74_R	82_D	0.627	0.91
160_E	232_R	0.624	0.91
75_L	84_S	0.622	0.91
233_K	236_R	0.622	0.91
171_V	185_S	0.621	0.91
76_E	79_S	0.619	0.90
111_T	115_L	0.619	0.90
16_Y	21_L	0.619	0.90
112_R	115_L	0.618	0.90
218_K	226_L	0.618	0.90
74_R	85_D	0.611	0.90
169_D	193_G	0.610	0.90
53_Q	111_T	0.606	0.89
74_R	78_F	0.606	0.89
191_Y	194_S	0.605	0.89
38_E	60_A	0.605	0.89
119_R	122_I	0.604	0.89
9_R	169_D	0.604	0.89
10_G	187_R	0.604	0.89
146_I	162_F	0.603	0.89
74_R	81_G	0.602	0.89
42_L	103_V	0.601	0.89
50_Q	111_T	0.601	0.89
79_S	84_S	0.600	0.89
99_R	117_E	0.600	0.89
103_V	107_T	0.598	0.89
38_E	97_V	0.594	0.89
196_F	238_V	0.594	0.89
76_E	83_F	0.589	0.88
102_Q	106_R	0.588	0.88
138_I	146_I	0.587	0.88
36_L	125_N	0.584	0.88
46_M	111_T	0.584	0.88
145_I	191_Y	0.579	0.87
131_V	245_N	0.577	0.87
76_E	82_D	0.577	0.87
70_L	80_K	0.576	0.87
168_L	196_F	0.576	0.87
35_P	95_G	0.576	0.87
138_I	199_L	0.574	0.87
23_N	26_N	0.573	0.87
182_K	185_S	0.571	0.87
85_D	93_Y	0.571	0.87
77_K	83_F	0.567	0.86
195_L	199_L	0.567	0.86
69_K	76_E	0.566	0.86
103_V	106_R	0.565	0.86
225_K	229_A	0.565	0.86
154_K	243_Y	0.561	0.86
207_N	211_L	0.561	0.86
69_K	74_R	0.561	0.86
81_G	84_S	0.560	0.85
72_E	75_L	0.559	0.85
134_T	241_V	0.559	0.85
29_R	34_N	0.558	0.85
30_E	34_N	0.556	0.85
172_V	181_R	0.556	0.85
215_E	218_K	0.553	0.85
203_Q	207_N	0.552	0.85
70_L	75_L	0.551	0.85
69_K	84_S	0.550	0.84
68_E	93_Y	0.550	0.84
185_S	193_G	0.550	0.84
53_Q	56_R	0.550	0.84
167_G	176_G	0.550	0.84
174_R	178_I	0.549	0.84
204_Y	227_Y	0.548	0.84
104_L	107_T	0.546	0.84
204_Y	218_K	0.546	0.84
193_G	228_T	0.544	0.84
168_L	232_R	0.544	0.84
243_Y	246_K	0.541	0.83
73_E	124_G	0.540	0.83
34_N	37_K	0.538	0.83
134_T	147_I	0.537	0.83
56_R	104_L	0.537	0.83
199_L	203_Q	0.535	0.83
40_V	148_G	0.533	0.83
76_E	84_S	0.532	0.82
22_E	28_A	0.528	0.82
45_F	52_K	0.527	0.82
7_D	23_N	0.527	0.82
45_F	48_D	0.524	0.82
167_G	193_G	0.522	0.81
42_L	45_F	0.522	0.81
56_R	59_R	0.520	0.81
9_R	164_A	0.519	0.81
106_R	117_E	0.518	0.81
68_E	71_S	0.517	0.81
39_L	121_M	0.517	0.81
131_V	135_I	0.516	0.81
32_V	35_P	0.514	0.80
66_D	82_D	0.510	0.80
7_D	16_Y	0.510	0.80
113_E	116_K	0.509	0.80
99_R	110_Q	0.508	0.80
42_L	104_L	0.506	0.79
229_A	232_R	0.505	0.79
132_L	138_I	0.503	0.79
201_E	205_S	0.503	0.79
218_K	221_L	0.503	0.79
77_K	82_D	0.500	0.78
203_Q	234_L	0.500	0.78

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1keaA	2	0.7888	92.8	0.911	Contact Map
2a1jB	2	0.3068	80.9	0.927	Contact Map
4glxA	1	0.5339	80.4	0.927	Contact Map
3fhfA	1	0.741	77.9	0.929	Contact Map
1pu6A	1	0.7769	77.4	0.93	Contact Map
2a1jA	1	0.2351	76.4	0.93	Contact Map
1z00A	1	0.3267	75.7	0.931	Contact Map
1z00B	1	0.2829	75.2	0.931	Contact Map
2duyA	1	0.255	75.1	0.931	Contact Map
2abkA	1	0.7769	73.8	0.932	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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