Q97V95 - SULSO - GREMLIN Contacts

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Q97V95 - Uncharacterized protein

UniProt:	Q97V95	Sequences: MKVIDIANPIVITIFPESSLKEAIDILSNNPSGRIVVLKEEKPIGIVSTRDVVASFSEYGTNIYDLKVKDIMSKDLVIVSPNDDVNHVVRIMLMNNIGGIPVVDNNVIVGIFTEREILKLIASSMFSGLVDSVMSSNIISIGEENTIMDAAKLMVMNNVRRLPVFSKDNRLVGIITAADIVKYLAKNKNIGKVLDAGTKNPITINRYYSILNAAKLMIEKRIGTLLVMENQKLVGIVTERDLMYAYINIA	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	250 (231)
Sequences:	5415 (3381)
Seq/√Len:	222.4

ECOLI - IMDH
BACSU - MGTE IMDH
HALSA - Q9HQD0 Q9HRK9 IMDH Q9HMW8
SULSO - Q97V95 Q97U20 Q7LXN6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
166_S	170_R	4.393	1.00
128_G	132_S	3.541	1.00
141_I	164_V	3.493	1.00
104_D	107_V	3.140	1.00
46_I	199_K	2.983	1.00
81_P	109_V	2.948	1.00
53_V	216_L	2.853	1.00
209_S	212_N	2.691	1.00
86_N	90_R	2.654	1.00
234_V	238_T	2.590	1.00
11_V	17_E	2.556	1.00
83_D	87_H	2.540	1.00
145_N	149_D	2.459	1.00
50_R	54_A	2.456	1.00
81_P	131_D	2.316	1.00
142_G	145_N	2.313	1.00
129_L	132_S	2.276	1.00
215_K	219_E	2.248	1.00
79_V	102_V	2.239	1.00
143_E	172_V	2.183	1.00
79_V	130_V	2.116	1.00
142_G	167_K	2.072	1.00
79_V	88_V	2.014	1.00
190_I	195_D	1.937	1.00
34_R	70_D	1.921	1.00
192_K	195_D	1.917	1.00
39_K	42_K	1.896	1.00
86_N	127_S	1.864	1.00
9_P	17_E	1.840	1.00
195_D	198_T	1.832	1.00
202_I	205_N	1.806	1.00
124_S	128_G	1.797	1.00
52_V	55_S	1.775	1.00
80_S	83_D	1.750	1.00
238_T	241_D	1.737	1.00
190_I	193_V	1.672	1.00
210_I	239_E	1.651	1.00
55_S	58_E	1.644	1.00
214_A	243_M	1.638	1.00
191_G	195_D	1.623	1.00
86_N	126_F	1.562	1.00
107_V	170_R	1.547	1.00
59_Y	62_N	1.532	1.00
68_V	150_A	1.531	1.00
141_I	145_N	1.530	1.00
191_G	194_L	1.517	1.00
84_D	87_H	1.492	1.00
149_D	152_K	1.484	1.00
194_L	198_T	1.478	1.00
51_D	54_A	1.468	1.00
158_N	182_K	1.462	1.00
82_N	131_D	1.455	1.00
102_V	130_V	1.455	1.00
193_V	196_A	1.431	1.00
192_K	198_T	1.426	1.00
79_V	83_D	1.424	1.00
203_T	206_R	1.411	1.00
192_K	197_G	1.404	1.00
155_V	185_A	1.395	1.00
191_G	196_A	1.388	1.00
153_L	162_L	1.366	1.00
190_I	194_L	1.355	1.00
87_H	90_R	1.353	1.00
106_N	170_R	1.351	1.00
177_A	181_V	1.341	1.00
107_V	169_N	1.302	1.00
12_I	224_T	1.301	1.00
214_A	225_L	1.298	1.00
12_I	20_L	1.298	1.00
144_E	167_K	1.291	1.00
150_A	162_L	1.287	1.00
104_D	109_V	1.286	1.00
12_I	23_A	1.261	1.00
89_V	93_L	1.231	1.00
191_G	197_G	1.226	1.00
46_I	224_T	1.218	1.00
66_L	69_K	1.218	1.00
146_T	149_D	1.217	1.00
68_V	175_I	1.201	1.00
140_S	169_N	1.194	1.00
89_V	126_F	1.191	1.00
114_E	118_L	1.187	1.00
208_Y	212_N	1.185	1.00
145_N	153_L	1.181	1.00
147_I	151_A	1.161	1.00
165_F	169_N	1.155	1.00
152_K	156_M	1.151	1.00
90_R	94_M	1.146	1.00
216_L	223_G	1.143	1.00
46_I	226_L	1.128	1.00
66_L	70_D	1.126	1.00
72_M	164_V	1.120	1.00
212_N	215_K	1.113	1.00
50_R	55_S	1.113	1.00
191_G	198_T	1.100	1.00
125_M	128_G	1.098	1.00
230_N	234_V	1.096	1.00
208_Y	211_L	1.093	1.00
44_I	224_T	1.093	1.00
239_E	242_L	1.088	1.00
169_N	172_V	1.081	1.00
203_T	207_Y	1.076	1.00
30_N	45_G	1.076	1.00
141_I	162_L	1.075	1.00
32_S	59_Y	1.066	1.00
88_V	92_M	1.056	1.00
216_L	219_E	1.054	1.00
9_P	19_S	1.052	1.00
80_S	104_D	1.046	1.00
147_I	187_N	1.045	1.00
217_M	223_G	1.027	1.00
194_L	197_G	1.027	1.00
192_K	196_A	1.025	1.00
237_V	241_D	1.020	1.00
227_V	240_R	1.009	1.00
16_P	19_S	1.008	1.00
152_K	155_V	0.998	1.00
234_V	241_D	0.995	1.00
80_S	105_N	0.994	1.00
240_R	244_Y	0.989	1.00
112_F	133_V	0.975	1.00
230_N	233_L	0.972	1.00
103_V	106_N	0.969	1.00
202_I	206_R	0.969	1.00
83_D	91_I	0.966	1.00
193_V	197_G	0.949	1.00
233_L	243_M	0.939	1.00
113_T	116_E	0.933	1.00
222_I	225_L	0.933	1.00
85_V	133_V	0.930	1.00
216_L	220_K	0.929	1.00
79_V	100_I	0.928	1.00
151_A	184_L	0.924	1.00
147_I	183_Y	0.920	0.99
99_G	111_I	0.918	0.99
211_L	242_L	0.913	0.99
229_E	236_I	0.912	0.99
151_A	183_Y	0.911	0.99
233_L	240_R	0.906	0.99
139_I	157_N	0.892	0.99
238_T	244_Y	0.890	0.99
82_N	129_L	0.887	0.99
85_V	128_G	0.887	0.99
217_M	226_L	0.885	0.99
101_P	171_L	0.885	0.99
239_E	244_Y	0.882	0.99
196_A	199_K	0.882	0.99
206_R	209_S	0.876	0.99
142_G	169_N	0.869	0.99
11_V	15_F	0.868	0.99
202_I	207_Y	0.865	0.99
233_L	237_V	0.859	0.99
220_K	223_G	0.856	0.99
227_V	243_M	0.852	0.99
227_V	241_D	0.847	0.99
154_M	162_L	0.847	0.99
190_I	196_A	0.843	0.99
212_N	216_L	0.842	0.99
7_A	10_I	0.842	0.99
11_V	14_I	0.831	0.99
40_E	44_I	0.829	0.99
231_Q	235_G	0.819	0.99
99_G	161_R	0.814	0.99
77_V	100_I	0.812	0.99
15_F	192_K	0.810	0.99
71_I	183_Y	0.807	0.99
91_I	95_N	0.807	0.99
218_I	223_G	0.807	0.99
219_E	222_I	0.806	0.99
88_V	130_V	0.801	0.99
217_M	220_K	0.795	0.98
219_E	223_G	0.792	0.98
241_D	244_Y	0.789	0.98
211_L	215_K	0.786	0.98
17_E	45_G	0.786	0.98
181_V	185_A	0.783	0.98
213_A	216_L	0.782	0.98
217_M	222_I	0.782	0.98
217_M	221_R	0.781	0.98
195_D	199_K	0.778	0.98
32_S	57_S	0.775	0.98
154_M	177_A	0.772	0.98
104_D	170_R	0.771	0.98
199_K	224_T	0.771	0.98
102_V	134_M	0.770	0.98
213_A	217_M	0.770	0.98
238_T	242_L	0.770	0.98
150_A	175_I	0.767	0.98
210_I	214_A	0.766	0.98
101_P	108_I	0.763	0.98
211_L	214_A	0.761	0.98
143_E	167_K	0.755	0.98
66_L	71_I	0.754	0.98
58_E	61_T	0.754	0.98
233_L	244_Y	0.753	0.98
218_I	222_I	0.753	0.98
217_M	228_M	0.753	0.98
206_R	210_I	0.748	0.98
97_I	100_I	0.748	0.98
116_E	135_S	0.744	0.98
32_S	62_N	0.741	0.98
110_G	134_M	0.740	0.98
230_N	236_I	0.736	0.98
234_V	237_V	0.734	0.97
103_V	171_L	0.732	0.97
155_V	158_N	0.728	0.97
68_V	71_I	0.723	0.97
141_I	153_L	0.719	0.97
204_I	214_A	0.718	0.97
24_I	50_R	0.718	0.97
166_S	172_V	0.712	0.97
141_I	150_A	0.710	0.97
92_M	100_I	0.709	0.97
124_S	133_V	0.709	0.97
232_K	235_G	0.708	0.97
71_I	175_I	0.707	0.97
237_V	240_R	0.707	0.97
220_K	226_L	0.705	0.97
223_G	226_L	0.704	0.97
236_I	240_R	0.703	0.97
85_V	124_S	0.701	0.97
78_I	103_V	0.696	0.97
88_V	112_F	0.696	0.97
91_I	94_M	0.692	0.96
147_I	180_I	0.688	0.96
231_Q	234_V	0.687	0.96
77_V	92_M	0.683	0.96
190_I	198_T	0.678	0.96
232_K	236_I	0.677	0.96
33_G	60_G	0.676	0.96
215_K	218_I	0.676	0.96
55_S	59_Y	0.671	0.96
191_G	199_K	0.670	0.96
90_R	126_F	0.663	0.95
235_G	239_E	0.660	0.95
81_P	104_D	0.660	0.95
89_V	117_I	0.658	0.95
68_V	147_I	0.657	0.95
180_I	184_L	0.656	0.95
108_I	140_S	0.656	0.95
151_A	180_I	0.655	0.95
78_I	106_N	0.654	0.95
207_Y	212_N	0.653	0.95
12_I	35_I	0.653	0.95
84_D	129_L	0.653	0.95
225_L	229_E	0.652	0.95
18_S	22_E	0.644	0.95
230_N	240_R	0.643	0.95
199_K	203_T	0.643	0.95
233_L	239_E	0.642	0.95
209_S	213_A	0.642	0.95
220_K	228_M	0.640	0.94
228_M	232_K	0.638	0.94
217_M	232_K	0.638	0.94
51_D	55_S	0.637	0.94
71_I	147_I	0.637	0.94
181_V	184_L	0.637	0.94
222_I	227_V	0.636	0.94
214_A	218_I	0.634	0.94
21_K	25_D	0.633	0.94
29_N	73_S	0.632	0.94
108_I	163_P	0.632	0.94
111_I	138_I	0.630	0.94
222_I	226_L	0.630	0.94
240_R	243_M	0.628	0.94
219_E	224_T	0.626	0.94
93_L	126_F	0.625	0.94
56_F	59_Y	0.625	0.94
160_R	177_A	0.621	0.94
8_N	37_V	0.621	0.94
229_E	235_G	0.620	0.94
228_M	231_Q	0.619	0.93
107_V	168_D	0.618	0.93
45_G	48_S	0.617	0.93
146_T	187_N	0.617	0.93
42_K	45_G	0.617	0.93
140_S	165_F	0.614	0.93
223_G	227_V	0.611	0.93
39_K	43_P	0.610	0.93
193_V	198_T	0.608	0.93
229_E	232_K	0.607	0.93
147_I	177_A	0.606	0.93
90_R	93_L	0.605	0.93
92_M	117_I	0.603	0.93
218_I	225_L	0.597	0.92
82_N	105_N	0.591	0.92
78_I	100_I	0.590	0.92
223_G	243_M	0.583	0.91
111_I	135_S	0.582	0.91
73_S	174_I	0.582	0.91
213_A	219_E	0.580	0.91
209_S	239_E	0.579	0.91
86_N	94_M	0.569	0.90
85_V	112_F	0.568	0.90
228_M	233_L	0.566	0.90
221_R	225_L	0.565	0.90
96_N	114_E	0.564	0.90
204_I	207_Y	0.564	0.90
85_V	130_V	0.563	0.90
77_V	97_I	0.562	0.90
34_R	38_L	0.558	0.89
103_V	170_R	0.554	0.89
216_L	242_L	0.553	0.89
49_T	235_G	0.551	0.89
215_K	222_I	0.551	0.89
205_N	208_Y	0.550	0.89
72_M	78_I	0.547	0.88
223_G	228_M	0.547	0.88
213_A	220_K	0.546	0.88
219_E	243_M	0.545	0.88
213_A	223_G	0.543	0.88
8_N	20_L	0.541	0.88
85_V	89_V	0.541	0.88
58_E	62_N	0.538	0.88
217_M	229_E	0.537	0.88
21_K	32_S	0.536	0.87
204_I	208_Y	0.536	0.87
41_E	186_K	0.534	0.87
143_E	166_S	0.532	0.87
13_T	42_K	0.532	0.87
16_P	22_E	0.530	0.87
126_F	129_L	0.529	0.87
225_L	228_M	0.528	0.87
235_G	238_T	0.528	0.87
227_V	236_I	0.527	0.87
101_P	161_R	0.527	0.87
74_K	172_V	0.526	0.86
219_E	242_L	0.525	0.86
99_G	160_R	0.525	0.86
67_K	71_I	0.525	0.86
233_L	236_I	0.521	0.86
229_E	233_L	0.520	0.86
89_V	164_V	0.520	0.86
221_R	226_L	0.518	0.86
220_K	229_E	0.515	0.85
185_A	189_N	0.514	0.85
99_G	113_T	0.513	0.85
146_T	183_Y	0.512	0.85
23_A	44_I	0.510	0.85
67_K	147_I	0.508	0.85
223_G	229_E	0.507	0.84
76_L	171_L	0.505	0.84
68_V	145_N	0.503	0.84
217_M	227_V	0.501	0.84
80_S	86_N	0.500	0.84
150_A	154_M	0.500	0.84

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2zy9A	2	0.984	100	0.341	Contact Map
2ouxA	2	0.74	100	0.425	Contact Map
3kxrA	3	0.66	100	0.483	Contact Map
2yvyA	2	0.72	100	0.5	Contact Map
3l2bA	2	0.464	99.9	0.572	Contact Map
1vr9A	1	0.48	99.9	0.582	Contact Map
3ddjA	1	0.992	99.9	0.583	Contact Map
4af0A	3	0.532	99.9	0.59	Contact Map
2yzqA	2	0.716	99.9	0.591	Contact Map
3usbA	4	0.968	99.9	0.592	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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