Q97V72 - SULSO - GREMLIN Contacts

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Q97V72 - Electron transfer flavoprotein, subunit alpha (EtfA)

UniProt:	Q97V72	Sequences: MKTVVFSESPVFLRMAAAVAQSFGGEVIGISDNENVKFVNKLYIIDKIDEDGIVDLISSLSPDVILTGNVRRDRAIAGRVAGRLKLPIATDVVSLKVEDKKVTLNRVAYSGMGLVTLESELPIVITIANTQLQPKELQPSVEKVQLKEGRVKIVGRKSTSSGVNLATADIVVGVGRGIGSKENVKYAEELAKALGGAVGGSRPVAAELNWVSEDRQIGLSGLRIRPKLYIALGISGQPQHIAGIRDSKIIVAVNIDKNAPILENADYLVVGNAIDFCKAMLSKLGKK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	287 (275)
Sequences:	4022 (2489)
Seq/√Len:	150.1

ECOLI - YDIR FIXB YGCQ
BACSU - ETFA
HALSA - Q9HND2
SULSO - Q97V72 Q97V19

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
271_G	276_F	5.253	1.00
262_L	268_L	3.835	1.00
232_L	253_V	3.584	1.00
169_D	227_K	3.567	1.00
173_G	234_I	3.436	1.00
67_T	77_A	3.316	1.00
171_V	224_I	2.818	1.00
232_L	277_C	2.743	1.00
174_V	184_V	2.717	1.00
193_A	281_L	2.644	1.00
12_F	36_V	2.586	1.00
10_P	34_E	2.377	1.00
211_V	215_R	2.213	1.00
200_G	211_V	2.172	1.00
185_K	189_E	2.166	1.00
241_I	264_N	2.149	1.00
249_I	267_Y	2.135	1.00
185_K	188_E	2.104	1.00
174_V	187_A	2.095	1.00
252_A	265_A	2.075	1.00
189_E	281_L	2.042	1.00
92_V	125_I	1.995	1.00
170_I	196_G	1.994	1.00
201_S	234_I	1.993	1.00
105_N	114_L	1.963	1.00
190_L	281_L	1.960	1.00
228_L	251_V	1.956	1.00
183_N	274_I	1.948	1.00
232_L	273_A	1.938	1.00
188_E	198_V	1.921	1.00
57_I	65_I	1.899	1.00
94_S	105_N	1.893	1.00
178_I	187_A	1.873	1.00
173_G	231_A	1.866	1.00
227_K	248_K	1.853	1.00
229_Y	240_H	1.816	1.00
187_A	232_L	1.763	1.00
12_F	62_P	1.762	1.00
279_A	282_S	1.753	1.00
252_A	261_I	1.752	1.00
190_L	277_C	1.750	1.00
66_L	125_I	1.737	1.00
224_I	244_I	1.726	1.00
21_Q	25_G	1.725	1.00
18_A	42_L	1.722	1.00
173_G	217_I	1.715	1.00
190_L	194_L	1.708	1.00
164_N	167_T	1.697	1.00
165_L	171_V	1.685	1.00
171_V	199_G	1.677	1.00
172_V	190_L	1.670	1.00
12_F	34_E	1.659	1.00
54_V	83_R	1.643	1.00
50_E	83_R	1.636	1.00
244_I	247_S	1.605	1.00
54_V	58_S	1.574	1.00
45_I	48_I	1.572	1.00
32_D	35_N	1.528	1.00
55_D	59_S	1.526	1.00
77_A	126_T	1.508	1.00
184_V	198_V	1.496	1.00
96_K	105_N	1.467	1.00
22_S	25_G	1.467	1.00
212_S	215_R	1.458	1.00
21_Q	143_K	1.451	1.00
59_S	142_E	1.430	1.00
189_E	193_A	1.418	1.00
199_G	217_I	1.403	1.00
188_E	192_K	1.390	1.00
29_G	137_L	1.379	1.00
193_A	284_L	1.371	1.00
231_A	240_H	1.368	1.00
55_D	58_S	1.363	1.00
186_Y	277_C	1.361	1.00
231_A	234_I	1.341	1.00
54_V	80_V	1.337	1.00
80_V	124_V	1.332	1.00
180_S	210_W	1.331	1.00
46_D	75_A	1.331	1.00
6_F	96_K	1.324	1.00
103_T	116_T	1.324	1.00
178_I	273_A	1.324	1.00
15_M	23_F	1.316	1.00
275_D	279_A	1.314	1.00
26_E	29_G	1.306	1.00
41_K	45_I	1.302	1.00
269_V	276_F	1.294	1.00
162_G	192_K	1.278	1.00
93_V	105_N	1.271	1.00
93_V	114_L	1.267	1.00
279_A	283_K	1.262	1.00
186_Y	189_E	1.256	1.00
24_G	28_I	1.253	1.00
178_I	183_N	1.234	1.00
16_A	52_G	1.233	1.00
18_A	39_V	1.225	1.00
200_G	206_A	1.222	1.00
28_I	138_Q	1.221	1.00
63_D	122_P	1.219	1.00
200_G	205_A	1.217	1.00
91_D	128_A	1.210	1.00
166_A	222_L	1.208	1.00
50_E	53_I	1.206	1.00
26_E	137_L	1.204	1.00
108_A	113_G	1.199	1.00
92_V	127_I	1.192	1.00
173_G	224_I	1.178	1.00
262_L	270_V	1.174	1.00
96_K	116_T	1.151	1.00
25_G	29_G	1.148	1.00
29_G	138_Q	1.134	1.00
51_D	55_D	1.121	0.99
86_L	124_V	1.105	0.99
173_G	201_S	1.102	0.99
41_K	52_G	1.100	0.99
88_I	124_V	1.098	0.99
47_K	50_E	1.093	0.99
55_D	147_K	1.083	0.99
273_A	278_K	1.082	0.99
84_L	124_V	1.078	0.99
177_G	210_W	1.072	0.99
271_G	275_D	1.070	0.99
181_K	211_V	1.067	0.99
186_Y	281_L	1.065	0.99
174_V	178_I	1.064	0.99
41_K	46_D	1.056	0.99
165_L	199_G	1.054	0.99
7_S	66_L	1.053	0.99
173_G	199_G	1.053	0.99
192_K	195_G	1.051	0.99
13_L	27_V	1.049	0.99
14_R	53_I	1.045	0.99
56_L	144_V	1.038	0.99
182_E	185_K	1.033	0.99
36_V	142_E	1.030	0.99
28_I	35_N	1.030	0.99
37_K	40_N	1.028	0.99
217_I	231_A	1.028	0.99
44_I	49_D	1.026	0.99
51_D	148_E	1.022	0.99
277_C	281_L	1.021	0.99
104_L	125_I	1.018	0.99
183_N	186_Y	1.011	0.99
77_A	88_I	1.010	0.99
203_P	208_L	1.008	0.99
88_I	126_T	1.003	0.99
9_S	63_D	0.996	0.99
17_A	20_A	0.992	0.99
174_V	211_V	0.988	0.99
53_I	80_V	0.980	0.99
225_R	246_D	0.979	0.99
36_V	60_L	0.977	0.99
50_E	79_R	0.974	0.99
191_A	198_V	0.974	0.99
31_S	136_E	0.973	0.99
179_G	274_I	0.972	0.98
23_F	26_E	0.970	0.98
197_A	215_R	0.970	0.98
33_N	36_V	0.966	0.98
253_V	276_F	0.963	0.98
48_I	51_D	0.952	0.98
74_R	91_D	0.947	0.98
43_Y	46_D	0.945	0.98
79_R	83_R	0.942	0.98
58_S	61_S	0.940	0.98
57_I	84_L	0.937	0.98
180_S	183_N	0.937	0.98
165_L	222_L	0.932	0.98
187_A	198_V	0.931	0.98
184_V	211_V	0.930	0.98
14_R	56_L	0.930	0.98
201_S	217_I	0.929	0.98
31_S	35_N	0.927	0.98
129_N	223_R	0.916	0.98
184_V	187_A	0.915	0.98
52_G	55_D	0.915	0.98
67_T	126_T	0.908	0.98
81_A	88_I	0.906	0.98
206_A	216_Q	0.905	0.98
89_A	92_V	0.905	0.98
20_A	38_F	0.903	0.98
41_K	44_I	0.896	0.97
95_L	104_L	0.894	0.97
34_E	142_E	0.893	0.97
49_D	53_I	0.892	0.97
99_D	118_E	0.889	0.97
50_E	55_D	0.877	0.97
230_I	253_V	0.874	0.97
20_A	23_F	0.866	0.97
203_P	206_A	0.860	0.97
50_E	80_V	0.857	0.97
186_Y	278_K	0.856	0.97
189_E	198_V	0.847	0.96
69_N	91_D	0.846	0.96
178_I	181_K	0.845	0.96
36_V	56_L	0.840	0.96
92_V	104_L	0.838	0.96
25_G	28_I	0.837	0.96
29_G	140_S	0.830	0.96
49_D	76_I	0.830	0.96
105_N	116_T	0.830	0.96
199_G	215_R	0.828	0.96
205_A	210_W	0.826	0.96
174_V	205_A	0.820	0.96
10_P	33_N	0.820	0.96
69_N	73_D	0.816	0.96
30_I	137_L	0.811	0.95
63_D	121_L	0.808	0.95
30_I	135_K	0.807	0.95
60_L	142_E	0.804	0.95
177_G	183_N	0.803	0.95
87_P	120_E	0.802	0.95
178_I	184_V	0.800	0.95
153_I	156_R	0.797	0.95
104_L	123_I	0.796	0.95
226_P	247_S	0.792	0.95
230_I	251_V	0.792	0.95
67_T	73_D	0.788	0.95
177_G	181_K	0.788	0.95
35_N	142_E	0.786	0.95
16_A	49_D	0.774	0.94
54_V	84_L	0.773	0.94
178_I	274_I	0.771	0.94
142_E	145_Q	0.769	0.94
37_K	143_K	0.767	0.94
77_A	80_V	0.762	0.94
48_I	53_I	0.760	0.94
181_K	210_W	0.756	0.93
186_Y	232_L	0.744	0.93
84_L	122_P	0.741	0.93
92_V	95_L	0.739	0.93
32_D	36_V	0.738	0.93
178_I	210_W	0.737	0.93
29_G	136_E	0.734	0.92
28_I	32_D	0.734	0.92
48_I	149_G	0.733	0.92
244_I	264_N	0.730	0.92
29_G	139_P	0.728	0.92
47_K	51_D	0.726	0.92
44_I	75_A	0.726	0.92
49_D	55_D	0.720	0.92
19_V	39_V	0.720	0.92
26_E	30_I	0.719	0.92
53_I	57_I	0.715	0.91
184_V	205_A	0.712	0.91
56_L	60_L	0.711	0.91
180_S	274_I	0.707	0.91
201_S	241_I	0.701	0.91
176_R	203_P	0.695	0.90
171_V	197_A	0.691	0.90
57_I	62_P	0.690	0.90
261_I	268_L	0.689	0.90
178_I	186_Y	0.687	0.90
106_R	117_L	0.687	0.90
73_D	76_I	0.686	0.90
99_D	123_I	0.686	0.90
174_V	200_G	0.684	0.89
201_S	231_A	0.684	0.89
107_V	128_A	0.684	0.89
272_N	275_D	0.680	0.89
231_A	261_I	0.675	0.89
177_G	186_Y	0.673	0.89
170_I	195_G	0.673	0.89
27_V	30_I	0.672	0.89
27_V	35_N	0.671	0.89
16_A	41_K	0.671	0.89
58_S	84_L	0.671	0.89
173_G	240_H	0.667	0.88
217_I	244_I	0.667	0.88
207_E	216_Q	0.666	0.88
224_I	247_S	0.665	0.88
38_F	52_G	0.662	0.88
110_S	128_A	0.660	0.88
44_I	76_I	0.659	0.88
193_A	196_G	0.659	0.88
13_L	16_A	0.658	0.88
141_V	144_V	0.656	0.87
184_V	200_G	0.653	0.87
67_T	80_V	0.650	0.87
88_I	92_V	0.649	0.87
97_V	118_E	0.647	0.87
89_A	104_L	0.645	0.87
181_K	185_K	0.643	0.86
40_N	145_Q	0.641	0.86
31_S	138_Q	0.638	0.86
104_L	127_I	0.636	0.86
241_I	263_E	0.633	0.85
92_V	97_V	0.628	0.85
47_K	53_I	0.625	0.85
276_F	279_A	0.618	0.84
128_A	219_L	0.616	0.84
202_R	216_Q	0.616	0.84
241_I	244_I	0.615	0.84
51_D	147_K	0.614	0.84
53_I	76_I	0.612	0.84
205_A	211_V	0.612	0.84
163_V	197_A	0.612	0.84
103_T	118_E	0.611	0.83
172_V	198_V	0.609	0.83
21_Q	147_K	0.608	0.83
170_I	227_K	0.608	0.83
201_S	242_A	0.607	0.83
87_P	115_V	0.606	0.83
133_Q	136_E	0.606	0.83
143_K	146_L	0.604	0.83
106_R	239_Q	0.604	0.83
249_I	266_D	0.603	0.83
178_I	205_A	0.603	0.83
28_I	140_S	0.602	0.83
50_E	54_V	0.602	0.83
220_S	238_P	0.602	0.83
174_V	232_L	0.602	0.83
184_V	219_L	0.598	0.82
120_E	123_I	0.598	0.82
173_G	229_Y	0.596	0.82
205_A	273_A	0.596	0.82
12_F	60_L	0.595	0.82
178_I	277_C	0.595	0.82
229_Y	234_I	0.593	0.82
89_A	196_G	0.593	0.82
200_G	216_Q	0.593	0.82
47_K	150_R	0.592	0.81
183_N	189_E	0.591	0.81
252_A	262_L	0.590	0.81
136_E	140_S	0.590	0.81
16_A	53_I	0.589	0.81
13_L	33_N	0.588	0.81
51_D	54_V	0.585	0.81
118_E	121_L	0.580	0.80
47_K	52_G	0.577	0.80
67_T	76_I	0.577	0.80
47_K	149_G	0.576	0.80
39_V	143_K	0.576	0.80
24_G	141_V	0.572	0.79
163_V	171_V	0.571	0.79
42_L	45_I	0.566	0.79
250_I	264_N	0.566	0.79
98_E	103_T	0.564	0.78
77_A	124_V	0.563	0.78
177_G	273_A	0.562	0.78
29_G	32_D	0.560	0.78
64_V	123_I	0.559	0.78
36_V	144_V	0.559	0.78
164_N	222_L	0.559	0.78
74_R	90_T	0.558	0.78
40_N	52_G	0.556	0.77
172_V	281_L	0.556	0.77
79_R	82_G	0.555	0.77
61_S	122_P	0.553	0.77
72_R	76_I	0.553	0.77
274_I	278_K	0.552	0.77
177_G	201_S	0.549	0.76
15_M	24_G	0.549	0.76
171_V	217_I	0.547	0.76
172_V	193_A	0.544	0.76
194_L	230_I	0.544	0.76
137_L	140_S	0.544	0.76
252_A	268_L	0.543	0.76
30_I	33_N	0.542	0.76
162_G	188_E	0.542	0.76
188_E	215_R	0.541	0.75
41_K	48_I	0.540	0.75
260_P	263_E	0.540	0.75
158_S	257_K	0.539	0.75
267_Y	274_I	0.537	0.75
25_G	38_F	0.537	0.75
28_I	141_V	0.536	0.75
49_D	72_R	0.536	0.75
22_S	26_E	0.534	0.74
7_S	11_V	0.534	0.74
172_V	194_L	0.533	0.74
162_G	165_L	0.533	0.74
81_A	124_V	0.533	0.74
183_N	273_A	0.533	0.74
53_I	59_S	0.530	0.74
7_S	10_P	0.530	0.74
170_I	194_L	0.530	0.74
190_L	193_A	0.529	0.74
177_G	270_V	0.527	0.74
41_K	49_D	0.527	0.74
51_D	149_G	0.527	0.74
187_A	217_I	0.527	0.74
47_K	55_D	0.527	0.74
198_V	215_R	0.526	0.73
174_V	198_V	0.525	0.73
50_E	59_S	0.523	0.73
8_E	11_V	0.522	0.73
65_I	124_V	0.522	0.73
170_I	230_I	0.522	0.73
257_K	260_P	0.521	0.73
55_D	145_Q	0.521	0.73
97_V	103_T	0.520	0.73
21_Q	38_F	0.519	0.72
188_E	196_G	0.518	0.72
19_V	147_K	0.517	0.72
234_I	247_S	0.514	0.72
21_Q	39_V	0.512	0.71
244_I	261_I	0.512	0.71
173_G	244_I	0.512	0.71
188_E	211_V	0.512	0.71
46_D	79_R	0.508	0.71
245_R	263_E	0.508	0.71
165_L	168_A	0.508	0.71
256_D	259_A	0.505	0.70
172_V	277_C	0.505	0.70
86_L	122_P	0.504	0.70
213_E	217_I	0.504	0.70
224_I	264_N	0.503	0.70
187_A	277_C	0.503	0.70
181_K	188_E	0.503	0.70
20_A	42_L	0.503	0.70
189_E	282_S	0.500	0.70

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4l2iA	1	0.993	100	0.049	Contact Map
1o97D	1	0.9686	100	0.068	Contact Map
1efvA	1	0.9791	100	0.069	Contact Map
1efpA	1	0.9652	100	0.087	Contact Map
3ih5A	6	0.5679	100	0.696	Contact Map
1efpB	1	0.6132	99.9	0.742	Contact Map
1efvB	1	0.6341	99.9	0.743	Contact Map
4l2iB	1	0.6725	99.9	0.746	Contact Map
1o97C	1	0.6376	99.9	0.747	Contact Map
3fetA	2	0.5017	99.9	0.748	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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