Q97V53 - SULSO - GREMLIN Contacts

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Q97V53 - Transposase ISC1316

UniProt:	Q97V53	Sequences: MKLACEIYNTLRWADIYFYQRDGKGLTQTELRQLALDLRKQDDEYKQVYSQVVQQIADRYYEARQRFFEGLARFPKEKKPHKYYSLVYPQYGWKILQVREIRKGKKKLITLKLSNLGTFKVIVHRDFPLDKVKRVVVKLTRSERIYITFVVDHEFPKLPNTGKVVAIDVGIEKLIVTSDGEYFPNLRPYEKALWKVKHLHRELSRKKFLSNNWFKAKVKLARAYEYLKNLRTDLYMKLGKWFAEHYDVVVMEDIHVKQLIGKSLRSLRRRLSDVAFSELRDLIKYQLEKYGKKLILVNPAYTSKTCARCGYVKEDLSLSDRVFVCSNCGWIADRDYNASLNILKGAGSERSLVPVELRPLPVPALRYWHGRAVK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	374 (331)
Sequences:	4585 (2902)
Seq/√Len:	159.5

ECOLI - INSQ
HALSA - Q9HSW9 Q9HSD4 Q9HHJ9 Q9HM87 Q9HHG8 Q9HSZ6 Q9HSW7 Q9HHU6 Q9HSY4 Q9HHX8
SULSO - Q97V53 Q97XA4 Q97V47 Q97XQ8 Q97YI2 Q97V25 Q97XP1 Q97Y44 Q97YW4 Q97YI5 Q97YU1 Q97U51 Q97XQ0 Q97U68 Q97XB6 Q97Y48 Q97Z61 Q97Y67 Q97UQ8 Q97YW9 Q97WY6 Q97X39 Q7LXD9 Q97WZ3 Q7LXH2 Q7LXH6 Q97YF7 Q97U09 Q7LX59 Q7LX75 Q97YK6 Y2105 Q7LXH9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
233_D	237_K	5.251	1.00
307_A	337_N	4.186	1.00
255_H	258_Q	4.177	1.00
287_L	294_L	3.531	1.00
174_L	279_L	2.948	1.00
280_R	296_L	2.931	1.00
251_M	283_I	2.910	1.00
250_V	346_A	2.895	1.00
240_K	244_E	2.828	1.00
278_E	282_L	2.707	1.00
317_S	320_D	2.580	1.00
135_V	147_I	2.501	1.00
260_I	272_S	2.426	1.00
193_L	269_R	2.398	1.00
301_Y	304_K	2.339	1.00
278_E	281_D	2.304	1.00
243_A	287_L	2.275	1.00
284_K	296_L	2.267	1.00
297_V	342_I	2.262	1.00
305_T	312_V	2.246	1.00
345_G	348_S	2.227	1.00
279_L	283_I	2.226	1.00
133_K	152_D	2.201	1.00
199_L	216_A	2.135	1.00
249_V	287_L	2.068	1.00
324_V	331_I	2.055	1.00
236_M	282_L	2.045	1.00
11_L	57_A	2.043	1.00
13_W	17_Y	2.027	1.00
192_A	230_L	2.020	1.00
236_M	285_Y	2.003	1.00
302_T	342_I	1.991	1.00
232_T	278_E	1.984	1.00
309_C	328_C	1.958	1.00
306_C	309_C	1.957	1.00
239_G	282_L	1.955	1.00
304_K	316_L	1.953	1.00
28_Q	32_R	1.937	1.00
251_M	294_L	1.922	1.00
309_C	325_C	1.893	1.00
16_I	74_F	1.880	1.00
29_T	32_R	1.864	1.00
216_A	220_L	1.860	1.00
28_Q	58_D	1.851	1.00
265_R	268_R	1.823	1.00
236_M	286_Q	1.801	1.00
284_K	288_E	1.800	1.00
321_R	334_R	1.795	1.00
211_N	214_F	1.784	1.00
247_D	292_K	1.780	1.00
325_C	328_C	1.760	1.00
308_R	330_W	1.729	1.00
236_M	239_G	1.727	1.00
174_L	234_L	1.724	1.00
235_Y	282_L	1.696	1.00
306_C	325_C	1.680	1.00
59_R	87_V	1.670	1.00
306_C	328_C	1.648	1.00
323_F	332_A	1.636	1.00
202_E	206_K	1.633	1.00
6_E	44_E	1.631	1.00
262_K	268_R	1.609	1.00
203_L	217_K	1.608	1.00
206_K	216_A	1.584	1.00
167_I	175_I	1.573	1.00
12_R	60_Y	1.569	1.00
235_Y	278_E	1.551	1.00
251_M	296_L	1.522	1.00
251_M	280_R	1.505	1.00
214_F	217_K	1.491	1.00
322_V	331_I	1.478	1.00
70_G	73_R	1.463	1.00
253_D	296_L	1.463	1.00
177_T	181_E	1.458	1.00
323_F	330_W	1.457	1.00
340_L	344_K	1.451	1.00
239_G	285_Y	1.448	1.00
176_V	336_Y	1.420	1.00
16_I	20_Q	1.419	1.00
66_R	72_A	1.419	1.00
111_L	147_I	1.411	1.00
311_Y	326_S	1.410	1.00
164_V	343_L	1.409	1.00
51_Q	89_P	1.400	1.00
17_Y	21_R	1.398	1.00
316_L	320_D	1.392	1.00
29_T	33_Q	1.390	1.00
61_Y	65_Q	1.372	1.00
209_L	213_W	1.370	1.00
165_V	177_T	1.368	1.00
239_G	286_Q	1.364	1.00
252_E	299_P	1.340	1.00
51_Q	90_Q	1.323	1.00
64_R	74_F	1.317	1.00
15_D	31_L	1.306	1.00
173_K	176_V	1.301	1.00
239_G	279_L	1.294	1.00
262_K	265_R	1.284	1.00
271_L	276_F	1.276	1.00
313_K	316_L	1.273	1.00
225_E	229_N	1.272	1.00
11_L	60_Y	1.269	1.00
318_L	321_R	1.267	1.00
199_L	202_E	1.263	1.00
284_K	294_L	1.240	1.00
307_A	340_L	1.222	1.00
140_T	143_E	1.210	1.00
142_S	225_E	1.204	1.00
27_T	30_E	1.202	1.00
195_K	199_L	1.197	1.00
164_V	248_V	1.192	1.00
165_V	246_Y	1.191	1.00
144_R	225_E	1.191	1.00
62_E	66_R	1.184	1.00
192_A	227_L	1.183	1.00
255_H	299_P	1.181	1.00
199_L	223_A	1.181	1.00
65_Q	69_E	1.167	1.00
140_T	144_R	1.166	1.00
243_A	290_Y	1.159	1.00
32_R	58_D	1.156	1.00
307_A	332_A	1.149	1.00
178_S	182_Y	1.147	1.00
232_T	282_L	1.144	1.00
138_K	148_T	1.128	1.00
196_V	220_L	1.128	1.00
55_Q	58_D	1.125	1.00
304_K	315_D	1.123	1.00
196_V	224_Y	1.117	1.00
304_K	314_E	1.114	1.00
203_L	220_L	1.113	1.00
241_W	245_H	1.104	1.00
167_I	177_T	1.103	1.00
33_Q	36_L	1.101	1.00
332_A	340_L	1.096	1.00
235_Y	279_L	1.093	0.99
14_A	34_L	1.093	0.99
169_V	276_F	1.088	0.99
112_K	118_T	1.084	0.99
28_Q	57_A	1.074	0.99
161_T	247_D	1.070	0.99
171_I	274_V	1.069	0.99
282_L	285_Y	1.064	0.99
313_K	323_F	1.064	0.99
332_A	336_Y	1.059	0.99
173_K	184_P	1.058	0.99
55_Q	89_P	1.055	0.99
203_L	216_A	1.052	0.99
4_A	56_I	1.052	0.99
336_Y	340_L	1.045	0.99
200_H	204_S	1.041	0.99
66_R	71_L	1.041	0.99
166_A	343_L	1.038	0.99
49_Y	52_V	1.034	0.99
18_F	26_L	1.030	0.99
317_S	334_R	1.026	0.99
35_A	57_A	1.024	0.99
169_V	280_R	1.021	0.99
302_T	345_G	1.018	0.99
243_A	292_K	1.016	0.99
244_E	290_Y	1.014	0.99
260_I	268_R	1.013	0.99
297_V	345_G	1.013	0.99
318_L	334_R	1.008	0.99
87_V	134_R	1.004	0.99
317_S	321_R	0.999	0.99
239_G	283_I	0.988	0.99
313_K	326_S	0.983	0.99
93_W	97_Q	0.983	0.99
18_F	22_D	0.981	0.99
305_T	345_G	0.980	0.99
182_Y	333_D	0.980	0.99
243_A	249_V	0.976	0.99
240_K	290_Y	0.974	0.99
32_R	57_A	0.973	0.99
88_Y	91_Y	0.972	0.99
92_G	114_S	0.972	0.99
112_K	115_N	0.960	0.99
8_Y	56_I	0.960	0.99
6_E	10_T	0.956	0.99
253_D	299_P	0.951	0.99
281_D	284_K	0.947	0.99
10_T	14_A	0.946	0.99
242_F	249_V	0.945	0.99
286_Q	289_K	0.940	0.98
39_R	53_V	0.937	0.98
313_K	317_S	0.936	0.98
59_R	136_V	0.931	0.98
262_K	266_S	0.929	0.98
123_V	126_D	0.928	0.98
36_L	39_R	0.927	0.98
51_Q	55_Q	0.923	0.98
11_L	31_L	0.922	0.98
316_L	323_F	0.919	0.98
226_Y	230_L	0.918	0.98
287_L	292_K	0.917	0.98
297_V	346_A	0.912	0.98
16_I	64_R	0.911	0.98
235_Y	275_A	0.909	0.98
94_K	97_Q	0.908	0.98
39_R	43_D	0.906	0.98
206_K	212_N	0.905	0.98
200_H	220_L	0.905	0.98
304_K	312_V	0.899	0.98
164_V	347_G	0.898	0.98
94_K	112_K	0.897	0.98
19_Y	23_G	0.892	0.98
87_V	136_V	0.892	0.98
165_V	178_S	0.890	0.98
110_T	118_T	0.889	0.98
321_R	333_D	0.886	0.98
235_Y	239_G	0.883	0.98
228_K	232_T	0.883	0.98
113_L	137_V	0.882	0.98
200_H	224_Y	0.881	0.98
12_R	64_R	0.880	0.98
250_V	342_I	0.880	0.98
192_A	196_V	0.879	0.98
196_V	204_S	0.877	0.98
67_F	75_P	0.873	0.98
13_W	16_I	0.872	0.98
196_V	227_L	0.872	0.98
48_V	51_Q	0.870	0.97
66_R	69_E	0.868	0.97
345_G	349_E	0.868	0.97
213_W	217_K	0.866	0.97
320_D	334_R	0.866	0.97
199_L	220_L	0.866	0.97
13_W	77_E	0.863	0.97
188_P	230_L	0.862	0.97
340_L	343_L	0.861	0.97
140_T	225_E	0.857	0.97
256_V	277_S	0.856	0.97
136_V	148_T	0.855	0.97
35_A	54_Q	0.852	0.97
203_L	213_W	0.850	0.97
54_Q	58_D	0.847	0.97
307_A	338_A	0.846	0.97
173_K	182_Y	0.843	0.97
322_V	332_A	0.843	0.97
338_A	341_N	0.842	0.97
248_V	293_K	0.840	0.97
223_A	227_L	0.838	0.97
320_D	323_F	0.837	0.97
67_F	74_F	0.835	0.97
311_Y	327_N	0.835	0.97
175_I	238_L	0.834	0.97
110_T	115_N	0.832	0.97
336_Y	339_S	0.829	0.97
197_K	200_H	0.823	0.96
45_Y	53_V	0.823	0.96
96_L	112_K	0.815	0.96
305_T	341_N	0.809	0.96
176_V	182_Y	0.808	0.96
202_E	205_R	0.808	0.96
199_L	219_K	0.808	0.96
66_R	70_G	0.807	0.96
197_K	201_R	0.806	0.96
305_T	314_E	0.805	0.96
91_Y	94_K	0.803	0.96
253_D	280_R	0.803	0.96
189_Y	193_L	0.802	0.96
14_A	17_Y	0.801	0.96
163_K	247_D	0.800	0.96
295_I	346_A	0.798	0.96
307_A	344_K	0.798	0.96
267_L	271_L	0.797	0.96
277_S	281_D	0.796	0.96
185_N	274_V	0.793	0.96
307_A	330_W	0.792	0.96
15_D	61_Y	0.792	0.96
176_V	183_F	0.792	0.96
11_L	35_A	0.791	0.96
198_H	202_E	0.789	0.96
217_K	221_A	0.789	0.96
234_L	238_L	0.787	0.95
185_N	188_P	0.782	0.95
219_K	222_R	0.781	0.95
164_V	250_V	0.771	0.95
240_K	286_Q	0.771	0.95
111_L	135_V	0.771	0.95
31_L	34_L	0.766	0.95
299_P	302_T	0.764	0.95
248_V	295_I	0.760	0.95
64_R	67_F	0.760	0.95
172_E	176_V	0.760	0.95
196_V	200_H	0.755	0.94
181_E	241_W	0.753	0.94
304_K	313_K	0.753	0.94
189_Y	192_A	0.749	0.94
165_V	249_V	0.744	0.94
14_A	18_F	0.743	0.94
204_S	220_L	0.741	0.94
265_R	272_S	0.741	0.94
55_Q	59_R	0.739	0.94
51_Q	91_Y	0.738	0.94
203_L	206_K	0.737	0.94
51_Q	54_Q	0.737	0.94
81_H	84_Y	0.736	0.94
201_R	205_R	0.730	0.93
316_L	334_R	0.729	0.93
337_N	341_N	0.729	0.93
171_I	270_R	0.727	0.93
137_V	147_I	0.724	0.93
30_E	33_Q	0.724	0.93
134_R	150_V	0.722	0.93
162_G	247_D	0.722	0.93
110_T	113_L	0.721	0.93
169_V	175_I	0.721	0.93
301_Y	345_G	0.718	0.93
308_R	340_L	0.712	0.92
64_R	68_F	0.710	0.92
179_D	246_Y	0.710	0.92
323_F	334_R	0.708	0.92
188_P	192_A	0.700	0.92
171_I	260_I	0.694	0.91
178_S	339_S	0.692	0.91
14_A	31_L	0.691	0.91
36_L	40_K	0.690	0.91
250_V	295_I	0.690	0.91
209_L	214_F	0.688	0.91
109_I	112_K	0.687	0.91
7_I	56_I	0.686	0.91
314_E	323_F	0.686	0.91
88_Y	93_W	0.684	0.91
202_E	219_K	0.680	0.91
92_G	95_I	0.678	0.90
55_Q	87_V	0.677	0.90
183_F	238_L	0.677	0.90
244_E	248_V	0.677	0.90
60_Y	64_R	0.676	0.90
344_K	347_G	0.676	0.90
158_L	292_K	0.673	0.90
22_D	25_G	0.672	0.90
256_V	276_F	0.672	0.90
258_Q	271_L	0.672	0.90
238_L	241_W	0.671	0.90
314_E	317_S	0.669	0.90
52_V	86_L	0.668	0.90
170_G	176_V	0.668	0.90
212_N	215_K	0.665	0.90
51_Q	92_G	0.658	0.89
183_F	186_L	0.655	0.89
15_D	18_F	0.655	0.89
242_F	246_Y	0.653	0.89
16_I	19_Y	0.652	0.89
112_K	116_L	0.650	0.89
116_L	120_K	0.648	0.88
254_I	299_P	0.647	0.88
49_Y	88_Y	0.646	0.88
8_Y	60_Y	0.645	0.88
111_L	114_S	0.643	0.88
311_Y	323_F	0.641	0.88
20_Q	23_G	0.640	0.88
160_N	163_K	0.639	0.88
88_Y	113_L	0.638	0.88
28_Q	31_L	0.637	0.88
57_A	61_Y	0.636	0.87
258_Q	299_P	0.636	0.87
175_I	279_L	0.635	0.87
140_T	145_I	0.633	0.87
210_S	213_W	0.633	0.87
183_F	234_L	0.633	0.87
192_A	223_A	0.632	0.87
174_L	274_V	0.631	0.87
190_E	269_R	0.631	0.87
144_R	229_N	0.628	0.87
316_L	321_R	0.628	0.87
182_Y	336_Y	0.628	0.87
39_R	45_Y	0.627	0.87
10_T	13_W	0.626	0.87
131_K	152_D	0.624	0.86
18_F	23_G	0.624	0.86
32_R	36_L	0.620	0.86
113_L	116_L	0.620	0.86
301_Y	305_T	0.619	0.86
249_V	283_I	0.619	0.86
97_Q	109_I	0.618	0.86
282_L	286_Q	0.617	0.86
117_G	120_K	0.616	0.86
186_L	234_L	0.616	0.86
197_K	204_S	0.614	0.86
48_V	53_V	0.614	0.86
24_K	30_E	0.614	0.86
14_A	38_L	0.613	0.85
25_G	31_L	0.613	0.85
315_D	320_D	0.610	0.85
10_T	44_E	0.602	0.84
250_V	343_L	0.601	0.84
121_V	124_H	0.601	0.84
50_S	89_P	0.600	0.84
15_D	25_G	0.596	0.84
303_S	316_L	0.594	0.84
175_I	183_F	0.594	0.84
34_L	38_L	0.594	0.84
53_V	56_I	0.593	0.84
36_L	50_S	0.593	0.84
163_K	246_Y	0.593	0.84
178_S	336_Y	0.592	0.83
58_D	89_P	0.590	0.83
206_K	210_S	0.590	0.83
15_D	22_D	0.590	0.83
88_Y	95_I	0.590	0.83
227_L	273_D	0.587	0.83
301_Y	312_V	0.587	0.83
238_L	279_L	0.586	0.83
7_I	53_V	0.585	0.83
116_L	119_F	0.584	0.83
31_L	35_A	0.583	0.83
231_R	273_D	0.583	0.83
39_R	42_D	0.582	0.82
26_L	31_L	0.582	0.82
109_I	115_N	0.578	0.82
324_V	330_W	0.577	0.82
85_S	136_V	0.577	0.82
342_I	345_G	0.576	0.82
280_R	284_K	0.574	0.82
192_A	226_Y	0.574	0.82
201_R	204_S	0.574	0.82
332_A	337_N	0.573	0.81
305_T	310_G	0.571	0.81
17_Y	20_Q	0.569	0.81
246_Y	249_V	0.569	0.81
232_T	236_M	0.569	0.81
19_Y	64_R	0.566	0.81
25_G	61_Y	0.566	0.81
85_S	89_P	0.566	0.81
111_L	117_G	0.565	0.81
56_I	60_Y	0.563	0.80
168_D	335_D	0.561	0.80
18_F	24_K	0.560	0.80
166_A	250_V	0.557	0.80
287_L	290_Y	0.557	0.80
190_E	193_L	0.556	0.79
8_Y	63_A	0.556	0.79
158_L	161_T	0.556	0.79
300_A	304_K	0.555	0.79
240_K	243_A	0.555	0.79
308_R	332_A	0.554	0.79
259_L	271_L	0.554	0.79
285_Y	289_K	0.553	0.79
275_A	278_E	0.550	0.79
57_A	60_Y	0.548	0.78
198_H	201_R	0.547	0.78
4_A	86_L	0.545	0.78
113_L	119_F	0.543	0.78
191_K	194_W	0.543	0.78
111_L	115_N	0.543	0.78
224_Y	228_K	0.541	0.78
326_S	334_R	0.540	0.77
313_K	334_R	0.539	0.77
166_A	178_S	0.539	0.77
224_Y	231_R	0.538	0.77
258_Q	262_K	0.538	0.77
110_T	114_S	0.536	0.77
172_E	321_R	0.536	0.77
35_A	39_R	0.535	0.77
13_W	20_Q	0.534	0.77
32_R	35_A	0.534	0.77
26_L	35_A	0.533	0.77
4_A	48_V	0.530	0.76
202_E	216_A	0.530	0.76
125_R	128_P	0.529	0.76
25_G	30_E	0.528	0.76
214_F	218_V	0.527	0.76
304_K	317_S	0.525	0.76
109_I	123_V	0.525	0.76
195_K	198_H	0.524	0.75
320_D	326_S	0.523	0.75
318_L	333_D	0.522	0.75
229_N	233_D	0.522	0.75
54_Q	57_A	0.522	0.75
168_D	303_S	0.522	0.75
15_D	26_L	0.521	0.75
174_L	235_Y	0.518	0.75
49_Y	90_Q	0.518	0.75
15_D	64_R	0.515	0.74
260_I	265_R	0.513	0.74
344_K	349_E	0.513	0.74
305_T	323_F	0.512	0.74
30_E	34_L	0.512	0.74
197_K	265_R	0.511	0.74
11_L	38_L	0.509	0.73
15_D	19_Y	0.508	0.73
323_F	326_S	0.508	0.73
283_I	294_L	0.507	0.73
68_F	71_L	0.506	0.73
10_T	38_L	0.506	0.73
169_V	256_V	0.505	0.73
155_F	288_E	0.504	0.73
268_R	272_S	0.504	0.73
140_T	146_Y	0.503	0.72
267_L	270_R	0.502	0.72
190_E	270_R	0.502	0.72
10_T	31_L	0.502	0.72
170_G	173_K	0.500	0.72

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1vhxA	2	0.3663	92.7	0.933	Contact Map
1nu0A	1	0.3476	92.3	0.934	Contact Map
1ffkW	1	0.1791	87.7	0.94	Contact Map
1iv0A	1	0.2594	86.3	0.941	Contact Map
4aybP	1	0.115	84.9	0.942	Contact Map
2k4xA	1	0.1444	82.3	0.944	Contact Map
1qypA	1	0.1203	81	0.945	Contact Map
1vq8Z	1	0.1765	80.4	0.945	Contact Map
3j20Y	1	0.1337	78.4	0.946	Contact Map
1vd4A	1	0.1497	74.1	0.948	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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