Q97V08 - SULSO - GREMLIN Contacts

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Q97V08 - Uncharacterized protein

UniProt:	Q97V08	Sequences: MNYKPLIKVLLALVILALVDYLASVILKTLTFYFPQISTYTSDTFTAITVIIIAIGGFYIIRILQGLIYSQLLTRLEKSTASSIKVALDIIFYTILVLVILAALKVNLTGVLVGGAVGGIVIGLAVQTIAQNILSGILVTSSRTIRLNDAVSLISWIWGNPIIGEISKISLLFTEVKTINGNVIKIPNSAFLGNTVFQKLESEHSLVYPYQLLVNADVPANDLLNLAKAYINDFFSKEKIQLPEIYFTGKNGGTNAFTVILHFNDIKQLNGILNLINGAFDKAYWELKKKG	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	291 (247)
Sequences:	11634 (8494)
Seq/√Len:	540.4

ECOLI - YBIO YNAI MSCM MSCK MSCS YBDG
BACSU - YHDY YKUT YFKC
HALSA - Q9HQH2 Q9HNG6 Q9HQ04
SULSO - Q97V08 Q97V66 Q7LXV0 Q97V46

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
281_D	285_W	3.778	1.00
57_G	96_L	3.544	1.00
221_N	282_K	3.518	1.00
152_S	163_I	3.362	1.00
167_S	177_K	3.111	1.00
228_K	232_N	3.029	1.00
249_G	256_A	2.949	1.00
224_L	278_G	2.901	1.00
222_D	287_L	2.803	1.00
283_A	287_L	2.701	1.00
283_A	286_E	2.665	1.00
234_F	275_L	2.545	1.00
150_A	163_I	2.460	1.00
225_N	229_A	2.455	1.00
248_T	258_T	2.436	1.00
251_N	256_A	2.408	1.00
211_Q	254_T	2.346	1.00
127_Q	131_Q	2.317	1.00
154_I	161_P	2.292	1.00
209_P	258_T	2.127	1.00
229_A	233_D	2.100	1.00
226_L	283_A	2.091	1.00
273_L	277_N	2.087	1.00
227_A	280_F	2.083	1.00
229_A	283_A	2.051	1.00
224_L	228_K	2.046	1.00
168_K	175_E	1.997	1.00
279_A	282_K	1.977	1.00
150_A	165_E	1.966	1.00
190_A	194_N	1.966	1.00
209_P	256_A	1.949	1.00
222_D	225_N	1.927	1.00
226_L	287_L	1.881	1.00
222_D	226_L	1.861	1.00
221_N	278_G	1.764	1.00
152_S	199_K	1.757	1.00
211_Q	256_A	1.753	1.00
247_F	255_N	1.716	1.00
275_L	279_A	1.710	1.00
167_S	183_V	1.683	1.00
282_K	286_E	1.652	1.00
178_T	184_I	1.643	1.00
233_D	279_A	1.640	1.00
142_S	172_L	1.639	1.00
230_Y	283_A	1.630	1.00
152_S	197_F	1.626	1.00
230_Y	279_A	1.591	1.00
261_L	276_I	1.587	1.00
212_L	223_L	1.570	1.00
152_S	161_P	1.548	1.00
69_Y	85_K	1.539	1.00
231_I	259_V	1.508	1.00
207_V	258_T	1.497	1.00
128_T	131_Q	1.475	1.00
154_I	195_T	1.457	1.00
226_L	229_A	1.457	1.00
150_A	179_I	1.456	1.00
65_Q	89_D	1.451	1.00
223_L	257_F	1.450	1.00
217_D	222_D	1.445	1.00
162_I	195_T	1.413	1.00
50_V	104_L	1.410	1.00
145_I	174_T	1.408	1.00
225_N	228_K	1.398	1.00
175_E	183_V	1.385	1.00
120_I	123_G	1.374	1.00
174_T	188_N	1.354	1.00
184_I	197_F	1.351	1.00
210_Y	280_F	1.339	1.00
234_F	279_A	1.330	1.00
233_D	283_A	1.322	1.00
154_I	194_N	1.321	1.00
271_G	275_L	1.318	1.00
212_L	216_A	1.317	1.00
59_Y	63_I	1.305	1.00
238_E	241_Q	1.298	1.00
265_D	268_Q	1.295	1.00
149_D	201_E	1.280	1.00
151_V	166_I	1.275	1.00
213_L	216_A	1.267	1.00
263_F	272_I	1.266	1.00
92_F	96_L	1.263	1.00
236_S	275_L	1.260	1.00
278_G	282_K	1.260	1.00
229_A	232_N	1.249	1.00
164_G	176_V	1.244	1.00
221_N	225_N	1.241	1.00
243_P	246_Y	1.240	1.00
57_G	61_I	1.236	1.00
211_Q	253_G	1.229	1.00
54_A	58_F	1.227	1.00
35_P	38_S	1.221	1.00
182_N	197_F	1.198	1.00
50_V	100_I	1.194	1.00
146_R	201_E	1.186	1.00
214_V	250_K	1.173	1.00
211_Q	251_N	1.171	1.00
190_A	193_G	1.150	1.00
178_T	182_N	1.147	1.00
46_T	49_T	1.141	1.00
284_Y	289_K	1.134	1.00
236_S	268_Q	1.127	1.00
114_G	117_V	1.124	1.00
215_N	281_D	1.116	1.00
209_P	249_G	1.112	1.00
183_V	201_E	1.101	1.00
211_Q	214_V	1.101	1.00
110_G	113_V	1.090	1.00
206_L	261_L	1.084	1.00
89_D	93_Y	1.084	1.00
65_Q	85_K	1.084	1.00
252_G	256_A	1.078	1.00
279_A	283_A	1.077	1.00
100_I	104_L	1.076	1.00
101_L	106_V	1.072	1.00
216_A	223_L	1.071	1.00
153_L	191_F	1.059	1.00
227_A	259_V	1.058	1.00
220_A	257_F	1.051	1.00
190_A	195_T	1.036	1.00
232_N	239_K	1.026	1.00
211_Q	252_G	1.025	1.00
228_K	245_I	1.009	1.00
58_F	62_R	1.009	1.00
276_I	280_F	1.000	1.00
248_T	256_A	0.993	1.00
269_L	273_L	0.992	1.00
221_N	224_L	0.983	1.00
266_I	269_L	0.967	1.00
118_G	122_I	0.957	1.00
61_I	89_D	0.951	1.00
112_L	116_A	0.946	1.00
274_N	278_G	0.942	1.00
220_A	224_L	0.929	1.00
53_I	103_A	0.928	1.00
173_F	185_K	0.928	1.00
58_F	93_Y	0.926	1.00
214_V	253_G	0.924	1.00
205_S	262_H	0.923	1.00
250_K	255_N	0.922	1.00
61_I	65_Q	0.914	1.00
82_S	86_V	0.908	1.00
240_I	243_P	0.908	1.00
226_L	230_Y	0.907	1.00
45_F	49_T	0.901	1.00
228_K	243_P	0.884	1.00
53_I	96_L	0.877	1.00
170_S	175_E	0.876	1.00
96_L	100_I	0.873	1.00
212_L	257_F	0.872	1.00
212_L	220_A	0.868	1.00
131_Q	189_S	0.867	1.00
231_I	261_L	0.867	1.00
85_K	89_D	0.861	1.00
177_K	183_V	0.860	1.00
126_V	134_L	0.855	1.00
143_R	146_R	0.854	1.00
264_N	268_Q	0.848	1.00
166_I	176_V	0.846	1.00
209_P	248_T	0.845	1.00
125_A	130_A	0.844	1.00
216_A	220_A	0.842	1.00
57_G	92_F	0.841	1.00
212_L	284_Y	0.840	1.00
162_I	184_I	0.840	1.00
206_L	273_L	0.838	1.00
251_N	254_T	0.837	1.00
41_T	45_F	0.837	1.00
247_F	277_N	0.835	1.00
250_K	277_N	0.835	1.00
181_G	262_H	0.832	1.00
245_I	259_V	0.831	1.00
224_L	274_N	0.830	1.00
204_H	269_L	0.825	1.00
117_V	126_V	0.822	1.00
246_Y	274_N	0.820	1.00
223_L	284_Y	0.819	1.00
61_I	92_F	0.811	1.00
165_E	177_K	0.811	1.00
246_Y	258_T	0.811	1.00
236_S	272_I	0.807	1.00
268_Q	271_G	0.802	1.00
129_I	135_S	0.800	1.00
135_S	192_L	0.791	1.00
43_D	47_A	0.791	1.00
227_A	284_Y	0.790	1.00
102_A	107_N	0.787	1.00
216_A	222_D	0.787	1.00
270_N	274_N	0.786	1.00
230_Y	276_I	0.786	1.00
151_V	176_V	0.785	1.00
138_L	192_L	0.782	1.00
37_I	40_Y	0.782	1.00
154_I	190_A	0.775	1.00
142_S	173_F	0.773	1.00
223_L	226_L	0.768	1.00
214_V	255_N	0.768	1.00
231_I	234_F	0.767	1.00
223_L	227_A	0.766	1.00
249_G	277_N	0.766	1.00
227_A	231_I	0.765	1.00
226_L	289_K	0.764	1.00
49_T	103_A	0.763	1.00
114_G	118_G	0.762	1.00
42_S	46_T	0.758	1.00
234_F	276_I	0.752	1.00
118_G	121_V	0.752	1.00
207_V	260_I	0.751	1.00
232_N	242_L	0.750	1.00
67_L	71_Q	0.747	1.00
210_Y	227_A	0.743	1.00
236_S	271_G	0.740	1.00
230_Y	234_F	0.740	1.00
228_K	231_I	0.740	1.00
36_Q	40_Y	0.738	1.00
275_L	278_G	0.734	1.00
48_I	52_I	0.722	1.00
113_V	116_A	0.716	1.00
206_L	263_F	0.715	1.00
43_D	46_T	0.710	1.00
94_T	98_L	0.709	1.00
226_L	286_E	0.705	1.00
248_T	273_L	0.704	1.00
242_L	246_Y	0.703	1.00
61_I	96_L	0.703	1.00
93_Y	97_V	0.701	1.00
177_K	181_G	0.701	1.00
214_V	252_G	0.696	1.00
78_K	81_A	0.689	1.00
230_Y	233_D	0.687	1.00
225_N	232_N	0.686	1.00
259_V	280_F	0.683	1.00
39_T	43_D	0.681	1.00
114_G	119_G	0.678	1.00
220_A	255_N	0.678	1.00
115_G	119_G	0.676	1.00
141_S	144_T	0.676	1.00
39_T	42_S	0.674	1.00
255_N	281_D	0.673	1.00
208_Y	277_N	0.672	1.00
183_V	202_S	0.672	1.00
68_I	71_Q	0.672	1.00
96_L	99_V	0.670	1.00
99_V	103_A	0.666	1.00
231_I	237_K	0.664	1.00
244_E	260_I	0.664	1.00
250_K	281_D	0.661	1.00
177_K	202_S	0.659	1.00
122_I	130_A	0.655	1.00
109_T	113_V	0.649	1.00
170_S	173_F	0.648	1.00
250_K	280_F	0.647	1.00
226_L	284_Y	0.646	1.00
180_N	205_S	0.646	1.00
231_I	276_I	0.644	1.00
153_L	162_I	0.640	1.00
52_I	56_G	0.640	1.00
153_L	186_I	0.639	1.00
132_N	189_S	0.633	1.00
122_I	134_L	0.633	1.00
220_A	247_F	0.630	1.00
131_Q	193_G	0.628	1.00
162_I	197_F	0.628	1.00
107_N	110_G	0.625	1.00
64_L	88_L	0.619	1.00
55_I	59_Y	0.616	1.00
145_I	191_F	0.615	1.00
214_V	254_T	0.613	1.00
205_S	260_I	0.612	1.00
212_L	289_K	0.611	1.00
235_F	238_E	0.611	1.00
64_L	92_F	0.609	1.00
220_A	282_K	0.609	1.00
95_I	99_V	0.605	1.00
112_L	121_V	0.603	1.00
208_Y	261_L	0.603	1.00
145_I	151_V	0.600	1.00
220_A	281_D	0.599	1.00
222_D	229_A	0.599	1.00
117_V	121_V	0.597	1.00
142_S	175_E	0.597	1.00
163_I	199_K	0.595	1.00
154_I	162_I	0.593	1.00
61_I	93_Y	0.592	1.00
231_I	243_P	0.591	1.00
53_I	57_G	0.590	1.00
152_S	162_I	0.589	1.00
36_Q	39_T	0.589	1.00
175_E	185_K	0.588	1.00
59_Y	62_R	0.588	1.00
228_K	242_L	0.588	1.00
72_L	81_A	0.588	1.00
217_D	285_W	0.587	0.99
127_Q	189_S	0.586	0.99
82_S	168_K	0.584	0.99
229_A	287_L	0.584	0.99
65_Q	69_Y	0.584	0.99
129_I	192_L	0.581	0.99
124_L	127_Q	0.579	0.99
240_I	246_Y	0.575	0.99
62_R	66_G	0.575	0.99
128_T	193_G	0.575	0.99
128_T	189_S	0.572	0.99
211_Q	255_N	0.572	0.99
237_K	272_I	0.571	0.99
90_I	94_T	0.571	0.99
38_S	42_S	0.565	0.99
186_I	195_T	0.564	0.99
69_Y	73_L	0.564	0.99
71_Q	74_T	0.564	0.99
151_V	191_F	0.562	0.99
79_S	140_T	0.560	0.99
120_I	128_T	0.560	0.99
224_L	245_I	0.560	0.99
139_V	145_I	0.558	0.99
210_Y	250_K	0.558	0.99
217_D	282_K	0.557	0.99
238_E	264_N	0.556	0.99
112_L	117_V	0.554	0.99
52_I	55_I	0.552	0.99
230_Y	287_L	0.551	0.99
214_V	251_N	0.551	0.99
77_E	146_R	0.550	0.99
35_P	40_Y	0.548	0.99
47_A	51_I	0.548	0.99
264_N	267_K	0.546	0.99
116_A	119_G	0.545	0.99
86_V	89_D	0.545	0.99
211_Q	250_K	0.545	0.99
266_I	270_N	0.544	0.99
135_S	189_S	0.543	0.99
117_V	120_I	0.543	0.99
272_I	275_L	0.541	0.99
246_Y	260_I	0.540	0.99
182_N	199_K	0.540	0.99
221_N	228_K	0.536	0.99
40_Y	43_D	0.535	0.99
277_N	280_F	0.531	0.99
215_N	285_W	0.530	0.99
119_G	125_A	0.529	0.99
247_F	250_K	0.526	0.99
180_N	207_V	0.526	0.99
224_L	257_F	0.524	0.99
223_L	283_A	0.524	0.99
79_S	83_S	0.523	0.99
216_A	255_N	0.522	0.99
62_R	65_Q	0.521	0.99
86_V	90_I	0.521	0.99
55_I	58_F	0.520	0.99
237_K	241_Q	0.520	0.99
237_K	261_L	0.519	0.99
124_L	129_I	0.518	0.99
234_F	237_K	0.518	0.99
118_G	125_A	0.517	0.99
42_S	45_F	0.515	0.99
227_A	245_I	0.515	0.99
50_V	54_A	0.514	0.99
231_I	245_I	0.513	0.99
258_T	274_N	0.512	0.99
86_V	168_K	0.511	0.99
270_N	273_L	0.507	0.99
138_L	172_L	0.507	0.99
108_L	114_G	0.506	0.99
78_K	82_S	0.506	0.99
44_T	48_I	0.506	0.99
81_A	85_K	0.505	0.99
51_I	62_R	0.504	0.98
189_S	193_G	0.501	0.98
226_L	231_I	0.501	0.98
267_K	270_N	0.500	0.98

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
5ajiA	3	0.8522	100	0.186	Contact Map
4hw9A	3	0.8247	100	0.19	Contact Map
3udcA	3	0.8282	100	0.221	Contact Map
1nz9A	1	0.1821	48.6	0.933	Contact Map
2e6zA	1	0.1924	36.9	0.938	Contact Map
2mi6A	1	0.1959	27	0.942	Contact Map
3cnrA	1	0.2921	26.4	0.942	Contact Map
2qn6B	1	0.2955	24.7	0.943	Contact Map
3ghfA	2	0.244	18	0.946	Contact Map
4hczA	1	0.1924	15.4	0.948	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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