Q97UW1 - SULSO - GREMLIN Contacts

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Q97UW1 - Second ORF in transposon ISC1225

UniProt:	Q97UW1	Sequences: MSPQTVRNYVEEQGTEVIEKLLESMRRISMEILKGVKEVDISIDWTTKTWYGKPVEGLGSSAKGNSWNYATATTKYQNMVLLLAFVPQVNGMSKDEIVKLLMEQIVGMGLKVGLVTLDAGFYTVEVLKFISQFKFVIGVPVGDVKIYEEFDGEYTTNSKRHKKEEQVKFRLLVYGKESLRRGEDRGVLREATNLDLPKREVLKLYNKVRSPIETSYRNIKAFLPFTSSTKFIFRELIFVLAMIFYSLYTVFKNVMTREEFRLLLILCFLDDLSDLKDFIFNLEETLINTIDLFLWR	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	296 (256)
Sequences:	198 (128)
Seq/√Len:	8.0

HALSA - Q9HHP9 O51989 O51959
SULSO - Q97UW1 Q97Y64 Q97Y40 Q97TV3 Q97YD0 Q97XW2 Q97XT0 Q97WW6 Q97YS0 Q7LXX4 Q97UV3 Q97V50

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
71_T	216_Y	5.976	1.00
117_L	215_S	5.014	0.99
43_I	98_V	3.850	0.97
112_V	136_V	3.578	0.95
7_R	233_F	3.291	0.92
60_S	66_S	3.103	0.89
82_L	216_Y	3.051	0.88
192_T	228_S	2.872	0.85
46_T	220_K	2.861	0.85
22_L	26_R	2.742	0.82
46_T	59_G	2.647	0.80
119_A	138_G	2.602	0.78
236_L	240_L	2.485	0.75
244_F	255_M	2.440	0.74
214_T	217_R	2.358	0.71
105_I	115_V	2.352	0.71
88_Q	116_T	2.322	0.70
72_A	84_A	2.220	0.66
181_R	185_R	2.202	0.65
118_D	209_R	2.170	0.64
50_W	246_S	2.154	0.64
260_F	263_L	2.062	0.60
122_Y	139_V	2.040	0.59
80_V	217_R	2.039	0.59
59_G	228_S	2.027	0.59
67_W	216_Y	2.000	0.58
222_F	245_Y	1.955	0.56
192_T	245_Y	1.920	0.54
41_I	105_I	1.903	0.54
78_N	128_K	1.880	0.53
42_S	58_L	1.860	0.52
220_K	245_Y	1.853	0.52
45_W	112_V	1.817	0.50
205_Y	212_I	1.731	0.47
216_Y	226_T	1.720	0.46
46_T	202_L	1.719	0.46
44_D	213_E	1.702	0.45
47_T	68_N	1.689	0.45
13_Q	55_V	1.681	0.45
82_L	242_M	1.657	0.44
98_V	101_L	1.634	0.43
224_P	237_I	1.618	0.42
192_T	210_S	1.600	0.41
18_I	21_L	1.589	0.41
120_G	247_L	1.572	0.40
122_Y	214_T	1.538	0.39
136_V	174_Y	1.509	0.38
10_V	65_N	1.483	0.37
216_Y	227_S	1.479	0.37
118_D	213_E	1.477	0.37
70_A	101_L	1.468	0.36
101_L	245_Y	1.447	0.35
69_Y	122_Y	1.419	0.34
193_N	245_Y	1.419	0.34
190_E	193_N	1.418	0.34
209_R	213_E	1.407	0.34
28_I	233_F	1.395	0.33
252_K	256_T	1.388	0.33
168_K	212_I	1.383	0.33
71_T	212_I	1.356	0.32
46_T	228_S	1.347	0.32
72_A	246_S	1.339	0.31
46_T	80_V	1.334	0.31
66_S	210_S	1.331	0.31
200_E	203_K	1.324	0.31
205_Y	213_E	1.320	0.31
64_G	261_R	1.312	0.30
71_T	238_F	1.310	0.30
88_Q	98_V	1.310	0.30
195_D	207_K	1.310	0.30
112_V	170_R	1.306	0.30
214_T	247_L	1.294	0.30
114_L	221_A	1.292	0.30
117_L	122_Y	1.283	0.29
220_K	228_S	1.239	0.28
192_T	222_F	1.237	0.28
11_E	102_M	1.233	0.28
220_K	238_F	1.229	0.28
190_E	228_S	1.211	0.27
70_A	135_F	1.205	0.27
135_F	252_K	1.203	0.27
109_G	236_L	1.202	0.27
50_W	58_L	1.201	0.27
164_E	187_V	1.201	0.27
110_L	155_T	1.197	0.27
6_V	224_P	1.196	0.27
98_V	105_I	1.195	0.27
112_V	216_Y	1.193	0.27
19_E	23_E	1.189	0.26
259_E	262_L	1.188	0.26
214_T	250_V	1.180	0.26
112_V	219_I	1.178	0.26
101_L	216_Y	1.177	0.26
14_G	18_I	1.176	0.26
127_L	135_F	1.161	0.26
71_T	239_V	1.161	0.26
44_D	118_D	1.158	0.25
105_I	135_F	1.131	0.25
225_F	246_S	1.117	0.24
206_N	246_S	1.113	0.24
228_S	238_F	1.107	0.24
112_V	182_G	1.098	0.24
110_L	115_V	1.097	0.24
52_G	69_Y	1.088	0.23
44_D	209_R	1.083	0.23
193_N	238_F	1.075	0.23
65_N	249_T	1.067	0.23
67_W	222_F	1.063	0.23
70_A	216_Y	1.054	0.22
131_S	161_H	1.053	0.22
13_Q	21_L	1.052	0.22
70_A	97_I	1.046	0.22
137_I	194_L	1.035	0.22
136_V	166_Q	1.034	0.22
6_V	9_Y	1.032	0.22
19_E	236_L	1.028	0.22
64_G	212_I	1.020	0.21
72_A	105_I	1.019	0.21
60_S	240_L	1.019	0.21
79_M	113_G	1.015	0.21
42_S	126_V	1.009	0.21
147_Y	150_F	0.999	0.21
206_N	211_P	0.998	0.21
88_Q	185_R	0.992	0.21
40_D	116_T	0.992	0.21
44_D	205_Y	0.991	0.21
81_L	143_D	0.989	0.21
112_V	195_D	0.988	0.21
92_M	136_V	0.985	0.20
42_S	75_K	0.983	0.20
189_R	249_T	0.976	0.20
249_T	252_K	0.975	0.20
80_V	113_G	0.970	0.20
9_Y	230_K	0.969	0.20
72_A	255_M	0.968	0.20
234_R	248_Y	0.967	0.20
190_E	236_L	0.966	0.20
42_S	59_G	0.964	0.20
122_Y	247_L	0.960	0.20
101_L	219_I	0.957	0.20
214_T	218_N	0.952	0.20
193_N	210_S	0.950	0.20
57_G	231_F	0.948	0.19
88_Q	198_K	0.938	0.19
47_T	120_G	0.938	0.19
39_V	197_P	0.938	0.19
127_L	191_A	0.934	0.19
233_F	237_I	0.932	0.19
192_T	220_K	0.931	0.19
74_T	248_Y	0.930	0.19
43_I	133_F	0.929	0.19
101_L	193_N	0.928	0.19
14_G	115_V	0.928	0.19
29_S	172_L	0.924	0.19
87_P	135_F	0.918	0.19
23_E	58_L	0.917	0.19
113_G	210_S	0.916	0.19
241_A	258_E	0.910	0.18
82_L	122_Y	0.895	0.18
117_L	120_G	0.895	0.18
76_Y	239_V	0.888	0.18
58_L	84_A	0.877	0.18
49_T	244_F	0.875	0.18
110_L	133_F	0.872	0.18
251_F	254_V	0.866	0.17
47_T	66_S	0.866	0.17
4_Q	7_R	0.864	0.17
70_A	126_V	0.863	0.17
43_I	70_A	0.863	0.17
24_S	126_V	0.862	0.17
64_G	102_M	0.860	0.17
69_Y	143_D	0.855	0.17
116_T	138_G	0.854	0.17
69_Y	175_G	0.853	0.17
29_S	218_N	0.852	0.17
174_Y	202_L	0.848	0.17
238_F	245_Y	0.833	0.17
176_K	181_R	0.830	0.17
121_F	213_E	0.826	0.17
64_G	112_V	0.824	0.16
5_T	42_S	0.823	0.16
210_S	236_L	0.821	0.16
219_I	238_F	0.819	0.16
149_E	153_E	0.816	0.16
89_V	102_M	0.815	0.16
4_Q	11_E	0.813	0.16
152_G	164_E	0.812	0.16
46_T	135_F	0.810	0.16
65_N	214_T	0.806	0.16
117_L	210_S	0.805	0.16
121_F	226_T	0.804	0.16
80_V	210_S	0.801	0.16
56_E	177_E	0.801	0.16
173_V	215_S	0.800	0.16
8_N	12_E	0.798	0.16
86_V	214_T	0.795	0.16
210_S	228_S	0.793	0.16
123_T	229_T	0.791	0.16
53_K	152_G	0.789	0.16
11_E	158_S	0.785	0.16
84_A	102_M	0.784	0.16
19_E	25_M	0.782	0.16
14_G	22_L	0.777	0.15
119_A	235_E	0.764	0.15
21_L	25_M	0.763	0.15
155_T	173_V	0.760	0.15
108_M	153_E	0.760	0.15
193_N	240_L	0.759	0.15
126_V	216_Y	0.757	0.15
193_N	222_F	0.756	0.15
82_L	219_I	0.751	0.15
74_T	88_Q	0.748	0.15
47_T	247_L	0.747	0.15
19_E	81_L	0.744	0.15
126_V	214_T	0.744	0.15
135_F	193_N	0.741	0.15
221_A	244_F	0.736	0.15
98_V	135_F	0.735	0.15
229_T	236_L	0.733	0.15
23_E	26_R	0.728	0.14
18_I	69_Y	0.728	0.14
10_V	18_I	0.728	0.14
48_K	84_A	0.728	0.14
127_L	205_Y	0.721	0.14
128_K	206_N	0.721	0.14
9_Y	12_E	0.721	0.14
86_V	100_L	0.720	0.14
223_L	235_E	0.716	0.14
45_W	88_Q	0.714	0.14
65_N	130_I	0.713	0.14
69_Y	205_Y	0.713	0.14
37_K	111_K	0.712	0.14
171_L	206_N	0.710	0.14
93_S	123_T	0.708	0.14
54_P	141_V	0.704	0.14
8_N	206_N	0.702	0.14
72_A	102_M	0.699	0.14
82_L	215_S	0.697	0.14
46_T	190_E	0.696	0.14
171_L	239_V	0.692	0.14
190_E	252_K	0.691	0.14
207_K	232_I	0.689	0.14
95_D	129_F	0.689	0.14
196_L	232_I	0.689	0.14
21_L	191_A	0.688	0.14
211_P	241_A	0.688	0.14
101_L	135_F	0.687	0.14
69_Y	236_L	0.685	0.14
10_V	77_Q	0.683	0.14
104_Q	224_P	0.683	0.14
83_L	90_N	0.680	0.13
15_T	27_R	0.679	0.13
113_G	241_A	0.678	0.13
80_V	139_V	0.677	0.13
206_N	216_Y	0.676	0.13
29_S	134_K	0.675	0.13
112_V	155_T	0.673	0.13
22_L	92_M	0.673	0.13
4_Q	104_Q	0.669	0.13
118_D	205_Y	0.668	0.13
21_L	24_S	0.666	0.13
36_V	109_G	0.665	0.13
25_M	207_K	0.665	0.13
67_W	71_T	0.665	0.13
33_L	201_V	0.664	0.13
5_T	220_K	0.662	0.13
184_D	191_A	0.662	0.13
120_G	140_P	0.660	0.13
67_W	191_A	0.658	0.13
49_T	157_N	0.658	0.13
213_E	234_R	0.657	0.13
135_F	238_F	0.657	0.13
50_W	229_T	0.657	0.13
127_L	137_I	0.654	0.13
163_K	168_K	0.653	0.13
211_P	217_R	0.652	0.13
7_R	13_Q	0.651	0.13
20_K	165_E	0.643	0.13
36_V	201_V	0.641	0.13
111_K	134_K	0.641	0.13
214_T	232_I	0.638	0.13
78_N	244_F	0.637	0.13
47_T	60_S	0.634	0.13
41_I	75_K	0.633	0.13
233_F	247_L	0.631	0.13
245_Y	252_K	0.627	0.12
44_D	212_I	0.627	0.12
141_V	151_D	0.627	0.12
4_Q	22_L	0.626	0.12
47_T	71_T	0.626	0.12
227_S	239_V	0.626	0.12
135_F	230_K	0.625	0.12
38_E	168_K	0.622	0.12
71_T	101_L	0.622	0.12
119_A	158_S	0.620	0.12
61_S	171_L	0.615	0.12
22_L	25_M	0.615	0.12
59_G	87_P	0.615	0.12
52_G	55_V	0.615	0.12
24_S	196_L	0.613	0.12
138_G	252_K	0.612	0.12
121_F	217_R	0.611	0.12
102_M	203_K	0.609	0.12
102_M	251_F	0.606	0.12
154_Y	233_F	0.600	0.12
205_Y	209_R	0.599	0.12
126_V	212_I	0.598	0.12
15_T	28_I	0.598	0.12
99_K	228_S	0.598	0.12
66_S	249_T	0.596	0.12
84_A	229_T	0.593	0.12
78_N	137_I	0.592	0.12
92_M	239_V	0.592	0.12
210_S	245_Y	0.592	0.12
29_S	171_L	0.588	0.12
189_R	243_I	0.588	0.12
136_V	205_Y	0.588	0.12
58_L	98_V	0.585	0.12
43_I	226_T	0.585	0.12
101_L	105_I	0.583	0.12
42_S	190_E	0.583	0.12
102_M	127_L	0.583	0.12
10_V	50_W	0.582	0.12
7_R	17_V	0.582	0.12
35_G	72_A	0.579	0.12
232_I	250_V	0.579	0.12
29_S	250_V	0.578	0.12
26_R	47_T	0.576	0.12
58_L	146_I	0.575	0.12
133_F	239_V	0.574	0.12
138_G	254_V	0.573	0.12
16_E	88_Q	0.572	0.12
51_Y	63_K	0.571	0.12
193_N	220_K	0.570	0.12
57_G	86_V	0.562	0.11
164_E	230_K	0.558	0.11
23_E	171_L	0.558	0.11
59_G	166_Q	0.558	0.11
87_P	244_F	0.556	0.11
43_I	156_T	0.555	0.11
236_L	242_M	0.553	0.11
14_G	91_G	0.552	0.11
61_S	66_S	0.552	0.11
127_L	130_I	0.551	0.11
84_A	117_L	0.550	0.11
52_G	122_Y	0.548	0.11
136_V	202_L	0.548	0.11
26_R	117_L	0.546	0.11
89_V	247_L	0.545	0.11
11_E	122_Y	0.544	0.11
19_E	117_L	0.542	0.11
193_N	228_S	0.541	0.11
7_R	11_E	0.541	0.11
42_S	74_T	0.540	0.11
138_G	226_T	0.539	0.11
69_Y	253_N	0.538	0.11
55_V	126_V	0.534	0.11
130_I	133_F	0.534	0.11
68_N	120_G	0.532	0.11
35_G	229_T	0.530	0.11
67_W	106_V	0.530	0.11
206_N	225_F	0.527	0.11
8_N	117_L	0.525	0.11
23_E	224_P	0.524	0.11
115_V	181_R	0.523	0.11
242_M	245_Y	0.523	0.11
58_L	91_G	0.522	0.11
202_L	252_K	0.522	0.11
20_K	64_G	0.519	0.11
172_L	201_V	0.519	0.11
89_V	165_E	0.519	0.11
121_F	205_Y	0.518	0.11
5_T	16_E	0.517	0.11
210_S	222_F	0.514	0.11
193_N	216_Y	0.514	0.11
185_R	188_L	0.510	0.11
76_Y	252_K	0.509	0.11
25_M	119_A	0.505	0.10
172_L	222_F	0.504	0.10
120_G	144_V	0.504	0.10
174_Y	206_N	0.503	0.10
152_G	260_F	0.502	0.10
43_I	126_V	0.502	0.10
196_L	240_L	0.500	0.10

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1musA	2	0.9189	99.3	0.844	Contact Map
1f6yA	2	0.6892	28.4	0.963	Contact Map
2yciX	2	0.6892	24.4	0.965	Contact Map
4xb4A	1	0.3345	14.3	0.968	Contact Map
4o1eA	2	0.6993	13.4	0.968	Contact Map
2ia0A	2	0.4054	8.9	0.971	Contact Map
1cxqA	2	0.4628	8.8	0.971	Contact Map
2vxzA	1	0.3851	8.3	0.971	Contact Map
4mq3A	1	0.4155	6.3	0.973	Contact Map
3flkA	3	0.1993	5.7	0.973	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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