Q97UT8 - SULSO - GREMLIN Contacts

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Q97UT8 - Phytoene synthetase (CrtB)

UniProt:	Q97UT8	Sequences: MIEKLEEIFKKGSVTYYNSSYFFPKKIREDVTKLYAFVRVFDDLVDSVPQKIEEYYNLRKLYQLEKSGYNTGNIVLQNFVELARRKGFEDSWIDAFLDAMESDIYKKVYYSIEELLKYMYGSAEVVGLFMMKILDLHNDSMYYARMLGRAMQFLNFIRDVQEDLYLGRQYLPVEDMEKFGIDKMECNEGFIEFIRYEISRYFEFEREAEKGFKFIPARYLVPIKTASEMYKWSALRIKKSPCIVLSKKVKPRRTKIVITVIKNFAGVYLWRSLNPII	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	277 (260)
Sequences:	3885 (2676)
Seq/√Len:	165.9

BACSU - YISP
HALSA - Q9HPV3 Q9HPE1 Q9HRI5
SULSO - Q97UT8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
202_F	238_K	4.792	1.00
111_S	114_E	4.773	1.00
179_F	196_Y	4.369	1.00
59_R	101_E	4.316	1.00
146_M	207_E	3.855	1.00
178_K	196_Y	3.491	1.00
175_D	197_E	2.723	1.00
202_F	235_L	2.575	1.00
91_S	95_A	2.505	1.00
63_Q	94_D	2.483	1.00
55_Y	59_R	2.474	1.00
81_E	85_R	2.440	1.00
30_D	86_K	2.387	1.00
106_K	114_E	2.352	1.00
110_Y	114_E	2.314	1.00
154_L	205_E	2.284	1.00
236_R	255_K	2.242	1.00
56_Y	101_E	2.237	1.00
182_D	185_E	2.161	1.00
243_I	246_S	2.135	1.00
111_S	174_E	2.076	1.00
63_Q	90_D	2.062	1.00
232_W	255_K	2.008	1.00
59_R	98_D	1.949	1.00
33_K	82_L	1.925	1.00
191_I	241_P	1.924	1.00
89_E	132_K	1.910	1.00
99_A	122_S	1.892	1.00
146_M	204_F	1.891	1.00
30_D	85_R	1.890	1.00
31_V	130_M	1.885	1.00
179_F	192_E	1.885	1.00
110_Y	173_V	1.884	1.00
52_I	105_Y	1.881	1.00
117_K	121_G	1.865	1.00
110_Y	177_E	1.834	1.00
56_Y	60_K	1.805	1.00
120_Y	145_R	1.780	1.00
161_Q	165_Y	1.683	1.00
99_A	121_G	1.649	1.00
112_I	200_R	1.647	1.00
261_I	265_A	1.613	1.00
175_D	179_F	1.604	1.00
209_E	231_K	1.600	1.00
57_N	61_L	1.593	1.00
27_I	133_I	1.588	1.00
90_D	94_D	1.571	1.00
102_S	117_K	1.570	1.00
198_I	234_A	1.533	1.00
34_L	133_I	1.526	1.00
113_E	116_L	1.523	1.00
88_F	129_F	1.512	1.00
122_S	156_F	1.507	1.00
173_V	177_E	1.502	1.00
61_L	64_L	1.488	1.00
128_L	145_R	1.483	1.00
116_L	149_R	1.482	1.00
175_D	196_Y	1.455	1.00
35_Y	39_R	1.450	1.00
198_I	238_K	1.440	1.00
149_R	204_F	1.425	1.00
34_L	129_F	1.422	1.00
64_L	69_Y	1.407	1.00
258_I	262_K	1.379	1.00
190_F	194_I	1.367	1.00
123_A	127_G	1.359	1.00
108_V	183_K	1.358	1.00
10_K	17_Y	1.341	1.00
152_Q	155_N	1.315	1.00
54_E	57_N	1.306	1.00
9_F	32_T	1.296	1.00
37_F	41_F	1.295	1.00
204_F	207_E	1.295	1.00
74_I	78_N	1.289	1.00
42_D	46_D	1.285	1.00
236_R	239_K	1.281	1.00
79_F	93_I	1.280	1.00
59_R	94_D	1.276	1.00
66_K	90_D	1.269	1.00
195_R	238_K	1.264	1.00
181_I	193_F	1.255	1.00
60_K	64_L	1.247	1.00
103_D	170_Y	1.229	1.00
42_D	159_D	1.221	1.00
140_S	214_F	1.215	1.00
193_F	197_E	1.214	1.00
197_E	200_R	1.202	1.00
198_I	242_C	1.199	1.00
185_E	189_G	1.169	1.00
202_F	231_K	1.164	1.00
46_D	159_D	1.161	1.00
179_F	193_F	1.160	1.00
120_Y	128_L	1.159	1.00
37_F	97_L	1.157	1.00
173_V	183_K	1.148	1.00
175_D	200_R	1.146	1.00
30_D	82_L	1.142	1.00
195_R	242_C	1.140	1.00
110_Y	174_E	1.135	1.00
191_I	243_I	1.133	1.00
188_E	192_E	1.125	1.00
15_T	229_M	1.122	1.00
120_Y	125_V	1.118	1.00
42_D	163_D	1.115	1.00
102_S	121_G	1.104	1.00
126_V	155_N	1.102	1.00
175_D	193_F	1.095	1.00
59_R	62_Y	1.090	1.00
232_W	235_L	1.084	1.00
175_D	178_K	1.083	1.00
41_F	97_L	1.080	1.00
57_N	60_K	1.079	1.00
63_Q	67_S	1.077	1.00
215_I	223_I	1.073	1.00
52_I	56_Y	1.070	0.99
98_D	117_K	1.066	0.99
150_A	154_L	1.058	0.99
181_I	189_G	1.044	0.99
155_N	158_R	1.042	0.99
92_W	132_K	1.040	0.99
55_Y	62_Y	1.036	0.99
27_I	218_R	1.022	0.99
174_E	178_K	1.017	0.99
124_E	148_G	1.014	0.99
88_F	93_I	1.007	0.99
34_L	93_I	1.006	0.99
80_V	84_R	1.004	0.99
61_L	73_N	0.990	0.99
209_E	227_S	0.988	0.99
7_E	11_K	0.981	0.99
255_K	258_I	0.980	0.99
143_Y	211_G	0.980	0.99
206_R	231_K	0.979	0.99
92_W	128_L	0.978	0.99
6_E	32_T	0.978	0.99
48_V	51_K	0.977	0.99
203_E	207_E	0.977	0.99
205_E	230_Y	0.975	0.99
162_E	166_L	0.974	0.99
89_E	92_W	0.970	0.99
240_S	247_K	0.970	0.99
112_I	197_E	0.970	0.99
24_P	27_I	0.964	0.99
164_L	186_C	0.958	0.99
112_I	175_D	0.954	0.99
59_R	63_Q	0.952	0.99
196_Y	200_R	0.947	0.99
158_R	230_Y	0.944	0.99
195_R	199_S	0.937	0.99
39_R	43_D	0.936	0.99
83_A	93_I	0.936	0.99
23_F	31_V	0.935	0.99
56_Y	59_R	0.923	0.98
39_R	127_G	0.921	0.98
12_G	43_D	0.915	0.98
86_K	133_I	0.899	0.98
53_E	56_Y	0.897	0.98
27_I	30_D	0.896	0.98
143_Y	214_F	0.894	0.98
123_A	152_Q	0.893	0.98
199_S	203_E	0.893	0.98
58_L	62_Y	0.891	0.98
62_Y	66_K	0.890	0.98
202_F	234_A	0.876	0.98
29_E	78_N	0.868	0.98
132_K	141_M	0.867	0.98
39_R	159_D	0.858	0.98
106_K	109_Y	0.856	0.98
73_N	82_L	0.855	0.98
112_I	196_Y	0.855	0.98
199_S	238_K	0.853	0.97
238_K	242_C	0.851	0.97
41_F	100_M	0.845	0.97
42_D	152_Q	0.844	0.97
103_D	168_R	0.843	0.97
16_Y	35_Y	0.839	0.97
39_R	46_D	0.837	0.97
181_I	187_N	0.836	0.97
157_I	234_A	0.834	0.97
42_D	155_N	0.828	0.97
77_Q	81_E	0.824	0.97
96_F	100_M	0.824	0.97
35_Y	123_A	0.821	0.97
68_G	71_T	0.819	0.97
78_N	81_E	0.817	0.97
46_D	155_N	0.817	0.97
115_L	153_F	0.815	0.97
254_T	258_I	0.808	0.97
53_E	57_N	0.807	0.96
190_F	244_V	0.806	0.96
39_R	42_D	0.806	0.96
169_Q	176_M	0.805	0.96
16_Y	39_R	0.803	0.96
144_A	148_G	0.801	0.96
232_W	259_T	0.800	0.96
251_P	256_I	0.799	0.96
95_A	98_D	0.797	0.96
73_N	77_Q	0.795	0.96
102_S	109_Y	0.795	0.96
208_A	227_S	0.794	0.96
123_A	155_N	0.792	0.96
157_I	237_I	0.791	0.96
169_Q	183_K	0.791	0.96
62_Y	93_I	0.789	0.96
35_Y	127_G	0.789	0.96
75_V	82_L	0.788	0.96
236_R	240_S	0.786	0.96
130_M	134_L	0.786	0.96
30_D	33_K	0.784	0.96
253_R	256_I	0.783	0.96
96_F	125_V	0.780	0.96
40_V	73_N	0.779	0.96
52_I	101_E	0.777	0.96
65_E	68_G	0.773	0.96
233_S	248_K	0.772	0.95
35_Y	155_N	0.767	0.95
78_N	82_L	0.764	0.95
161_Q	245_L	0.761	0.95
39_R	155_N	0.761	0.95
163_D	170_Y	0.758	0.95
212_F	227_S	0.755	0.95
14_V	18_N	0.753	0.95
82_L	86_K	0.750	0.95
29_E	33_K	0.746	0.95
108_V	167_G	0.742	0.94
16_Y	155_N	0.742	0.94
123_A	126_V	0.741	0.94
16_Y	126_V	0.739	0.94
235_L	238_K	0.733	0.94
210_K	213_K	0.731	0.94
25_K	29_E	0.730	0.94
159_D	163_D	0.728	0.94
16_Y	123_A	0.726	0.94
57_N	73_N	0.723	0.94
150_A	205_E	0.720	0.93
212_F	224_K	0.718	0.93
61_L	76_L	0.717	0.93
124_E	149_R	0.717	0.93
26_K	85_R	0.716	0.93
41_F	58_L	0.714	0.93
16_Y	127_G	0.711	0.93
207_E	210_K	0.709	0.93
142_Y	207_E	0.708	0.93
181_I	190_F	0.706	0.93
112_I	116_L	0.706	0.93
152_Q	159_D	0.704	0.93
112_I	204_F	0.703	0.93
215_I	220_L	0.701	0.93
54_E	61_L	0.700	0.93
98_D	101_E	0.699	0.92
192_E	195_R	0.696	0.92
38_V	126_V	0.690	0.92
124_E	131_M	0.684	0.92
7_E	10_K	0.683	0.92
254_T	261_I	0.683	0.92
142_Y	146_M	0.683	0.92
153_F	200_R	0.682	0.92
219_Y	222_P	0.680	0.91
76_L	80_V	0.680	0.91
9_F	19_S	0.680	0.91
98_D	121_G	0.671	0.91
152_Q	163_D	0.670	0.91
187_N	246_S	0.670	0.91
44_L	54_E	0.669	0.91
92_W	120_Y	0.668	0.91
176_M	193_F	0.666	0.91
146_M	149_R	0.662	0.90
237_I	244_V	0.659	0.90
127_G	152_Q	0.656	0.90
96_F	151_M	0.654	0.90
99_A	156_F	0.654	0.90
103_D	118_Y	0.650	0.89
27_I	216_P	0.648	0.89
148_G	158_R	0.646	0.89
42_D	123_A	0.646	0.89
161_Q	240_S	0.646	0.89
154_L	201_Y	0.645	0.89
95_A	121_G	0.645	0.89
79_F	83_A	0.644	0.89
9_F	13_S	0.642	0.89
39_R	123_A	0.641	0.89
240_S	243_I	0.640	0.89
130_M	223_I	0.638	0.89
223_I	226_A	0.637	0.89
127_G	155_N	0.635	0.88
62_Y	90_D	0.635	0.88
73_N	80_V	0.635	0.88
38_V	122_S	0.634	0.88
67_S	90_D	0.632	0.88
101_E	104_I	0.631	0.88
154_L	233_S	0.631	0.88
198_I	202_F	0.630	0.88
35_Y	42_D	0.626	0.88
141_M	145_R	0.625	0.88
181_I	185_E	0.622	0.87
196_Y	199_S	0.622	0.87
38_V	129_F	0.620	0.87
232_W	236_R	0.619	0.87
172_P	197_E	0.619	0.87
245_L	249_V	0.616	0.87
231_K	235_L	0.605	0.86
123_A	159_D	0.604	0.86
68_G	72_G	0.602	0.86
39_R	163_D	0.601	0.85
52_I	104_I	0.599	0.85
43_D	47_S	0.595	0.85
147_L	227_S	0.594	0.85
125_V	148_G	0.594	0.85
131_M	211_G	0.594	0.85
108_V	173_V	0.593	0.85
195_R	198_I	0.593	0.85
211_G	215_I	0.589	0.84
213_K	224_K	0.586	0.84
56_Y	62_Y	0.586	0.84
101_E	105_Y	0.585	0.84
35_Y	118_Y	0.580	0.83
39_R	126_V	0.579	0.83
41_F	130_M	0.578	0.83
56_Y	105_Y	0.575	0.83
155_N	159_D	0.574	0.83
15_T	45_V	0.573	0.83
22_F	225_T	0.573	0.83
157_I	198_I	0.573	0.83
128_L	132_K	0.572	0.83
39_R	152_Q	0.572	0.83
155_N	163_D	0.571	0.82
46_D	152_Q	0.571	0.82
127_G	151_M	0.570	0.82
251_P	259_T	0.568	0.82
200_R	203_E	0.566	0.82
234_A	238_K	0.563	0.82
38_V	43_D	0.560	0.81
233_S	251_P	0.560	0.81
222_P	226_A	0.559	0.81
200_R	204_F	0.557	0.81
96_F	129_F	0.556	0.81
123_A	170_Y	0.556	0.81
216_P	220_L	0.555	0.81
142_Y	247_K	0.553	0.80
202_F	206_R	0.552	0.80
205_E	234_A	0.551	0.80
154_L	234_A	0.550	0.80
252_R	258_I	0.550	0.80
82_L	85_R	0.550	0.80
153_F	197_E	0.549	0.80
15_T	158_R	0.548	0.80
248_K	251_P	0.547	0.80
54_E	58_L	0.547	0.80
203_E	206_R	0.546	0.80
180_G	183_K	0.546	0.80
35_Y	46_D	0.544	0.79
124_E	156_F	0.542	0.79
16_Y	46_D	0.537	0.79
201_Y	234_A	0.536	0.78
159_D	168_R	0.536	0.78
95_A	128_L	0.535	0.78
171_L	197_E	0.534	0.78
46_D	118_Y	0.534	0.78
131_M	141_M	0.532	0.78
156_F	163_D	0.532	0.78
38_V	226_A	0.531	0.78
39_R	151_M	0.530	0.78
193_F	196_Y	0.529	0.77
6_E	10_K	0.526	0.77
251_P	255_K	0.524	0.77
134_L	219_Y	0.524	0.77
153_F	204_F	0.524	0.77
212_F	215_I	0.524	0.77
41_F	134_L	0.523	0.77
100_M	122_S	0.521	0.76
75_V	83_A	0.520	0.76
122_S	126_V	0.518	0.76
66_K	93_I	0.517	0.76
22_F	28_R	0.516	0.76
224_K	263_N	0.516	0.76
124_E	128_L	0.515	0.76
192_E	199_S	0.514	0.75
14_V	20_S	0.513	0.75
181_I	186_C	0.512	0.75
91_S	132_K	0.512	0.75
127_G	163_D	0.511	0.75
83_A	86_K	0.510	0.75
151_M	155_N	0.510	0.75
124_E	146_M	0.508	0.75
122_S	155_N	0.507	0.74
206_R	209_E	0.506	0.74
226_A	230_Y	0.505	0.74
145_R	149_R	0.504	0.74
26_K	29_E	0.503	0.74
123_A	163_D	0.500	0.73

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4hd1A	1	0.8809	100	0.118	Contact Map
3acxA	1	0.9567	100	0.121	Contact Map
3we9A	1	0.8231	100	0.15	Contact Map
3wcaA	1	0.9964	100	0.196	Contact Map
3vj8A	1	0.9928	100	0.22	Contact Map
1wy0A	2	0.8339	95.9	0.901	Contact Map
4dhdA	2	0.8267	95.5	0.904	Contact Map
3oyrA	2	0.8375	95.4	0.904	Contact Map
3ts7A	2	0.7726	95.4	0.904	Contact Map
4lfgA	2	0.769	95.4	0.904	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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