Q97UT0 - SULSO - GREMLIN Contacts

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Q97UT0 - Uncharacterized protein

UniProt:	Q97UT0	Sequences: MMLDLIYLSVIIFIASIPTGAFAYGFSSTATPLLLIRYTNKQISPILNFIEMFQNLTNVILNFKNYSIRSLVFTIFGTPGVLIGSIVGAYILKISANETLFKVIVYSILIPLILLQAAGFRKEIGLEKWKKIGYLVTFPVGVLYGVTTISGPPLALIINNQGLTKQEFKFAIAVLRTVESVSTFGAYLTLGIFNPQVIYYATVTSPAVIAGMLIGIFMGNVIKHKEDFRRLVMSFDSWISGYGLSFNLAKVVDYGVWYWPMTAIIAIDLYLLLRYIKGRRRLLK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	284 (234)
Sequences:	12465 (8884)
Seq/√Len:	580.8

ECOLI - YFCA
BACSU - YTNM YUNE YRKJ YJNA YDHB
HALSA - O54662
SULSO - Q97UT0 Q97WF4 Q981D0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
245_S	249_A	3.637	1.00
226_E	230_R	3.483	1.00
84_G	138_F	3.421	1.00
17_I	218_M	3.392	1.00
227_D	230_R	3.084	1.00
67_S	167_E	3.007	1.00
9_S	207_A	2.908	1.00
91_I	142_V	2.879	1.00
80_G	134_Y	2.587	1.00
11_I	33_L	2.463	1.00
217_F	221_V	2.438	1.00
67_S	70_S	2.393	1.00
13_F	211_G	2.381	1.00
224_H	227_D	2.306	1.00
213_L	217_F	2.236	1.00
69_R	72_V	2.224	1.00
164_T	167_E	2.216	1.00
114_L	118_A	2.087	1.00
71_L	174_V	2.052	1.00
106_Y	243_G	2.015	1.00
152_P	156_L	2.008	1.00
244_L	248_L	1.987	1.00
53_F	205_S	1.966	1.00
68_I	72_V	1.910	1.00
75_I	178_V	1.901	1.00
69_R	73_F	1.823	1.00
63_F	68_I	1.817	1.00
113_I	240_S	1.797	1.00
57_T	209_I	1.777	1.00
113_I	243_G	1.749	1.00
48_N	183_T	1.738	1.00
110_I	243_G	1.671	1.00
57_T	213_L	1.655	1.00
81_V	179_E	1.652	1.00
12_I	204_T	1.641	1.00
199_Y	203_V	1.634	1.00
90_Y	95_S	1.628	1.00
46_I	201_A	1.601	1.00
49_F	201_A	1.583	1.00
70_S	163_L	1.583	1.00
217_F	220_N	1.572	1.00
113_I	233_M	1.539	1.00
107_S	111_P	1.534	1.00
89_A	186_A	1.517	1.00
75_I	79_P	1.510	1.00
207_A	211_G	1.508	1.00
39_T	42_Q	1.487	1.00
86_I	186_A	1.479	1.00
15_A	29_T	1.478	1.00
230_R	234_S	1.476	1.00
131_K	135_L	1.466	1.00
140_V	153_P	1.466	1.00
13_F	210_A	1.462	1.00
84_G	88_G	1.456	1.00
72_V	79_P	1.451	1.00
61_L	216_I	1.448	1.00
72_V	75_I	1.441	1.00
87_V	138_F	1.430	1.00
106_Y	247_N	1.424	1.00
80_G	137_T	1.420	1.00
202_T	206_P	1.404	1.00
65_N	220_N	1.403	1.00
60_I	63_F	1.396	1.00
234_S	241_G	1.382	1.00
131_K	134_Y	1.381	1.00
6_I	10_V	1.361	1.00
80_G	84_G	1.358	1.00
42_Q	45_P	1.352	1.00
58_N	173_A	1.348	1.00
17_I	214_I	1.340	1.00
71_L	171_A	1.338	1.00
117_A	233_M	1.313	1.00
99_T	103_V	1.309	1.00
79_P	83_I	1.309	1.00
90_Y	93_K	1.298	1.00
12_I	207_A	1.274	1.00
62_N	219_G	1.256	1.00
82_L	182_S	1.253	1.00
100_L	103_V	1.234	1.00
227_D	231_L	1.224	1.00
108_I	147_T	1.223	1.00
100_L	104_I	1.222	1.00
240_S	244_L	1.219	1.00
136_V	157_I	1.211	1.00
154_L	175_L	1.208	1.00
86_I	90_Y	1.205	1.00
129_W	132_I	1.202	1.00
138_F	142_V	1.200	1.00
56_L	60_I	1.184	1.00
245_S	248_L	1.178	1.00
152_P	155_A	1.177	1.00
56_L	177_T	1.176	1.00
59_V	174_V	1.171	1.00
5_L	203_V	1.154	1.00
110_I	240_S	1.147	1.00
97_N	100_L	1.141	1.00
186_A	190_L	1.128	1.00
185_G	189_T	1.109	1.00
118_A	121_R	1.104	1.00
206_P	210_A	1.096	1.00
58_N	212_M	1.095	1.00
209_I	213_L	1.083	1.00
10_V	14_I	1.082	1.00
165_K	225_K	1.082	1.00
13_F	214_I	1.082	1.00
98_E	102_K	1.080	1.00
52_M	181_V	1.075	1.00
211_G	214_I	1.072	1.00
181_V	185_G	1.069	1.00
66_Y	171_A	1.062	1.00
62_N	170_F	1.059	1.00
121_R	124_I	1.059	1.00
111_P	115_L	1.057	1.00
83_I	86_I	1.054	1.00
153_P	156_L	1.053	1.00
87_V	90_Y	1.052	1.00
42_Q	194_N	1.046	1.00
59_V	63_F	1.046	1.00
135_L	139_P	1.038	1.00
73_F	79_P	1.037	1.00
12_I	30_A	1.034	1.00
132_I	135_L	1.027	1.00
8_L	204_T	1.025	1.00
166_Q	219_G	1.021	1.00
85_S	183_T	1.010	1.00
225_K	229_R	1.002	1.00
241_G	245_S	1.000	1.00
211_G	215_G	0.996	1.00
102_K	249_A	0.977	1.00
7_Y	10_V	0.976	1.00
80_G	83_I	0.973	1.00
214_I	217_F	0.969	1.00
68_I	71_L	0.959	1.00
73_F	80_G	0.957	1.00
21_A	222_I	0.956	1.00
194_N	199_Y	0.955	1.00
12_I	16_S	0.953	1.00
103_V	107_S	0.952	1.00
35_L	40_N	0.951	1.00
110_I	114_L	0.947	1.00
114_L	240_S	0.945	1.00
103_V	247_N	0.940	1.00
99_T	102_K	0.935	1.00
144_Y	150_S	0.931	1.00
202_T	205_S	0.917	1.00
57_T	212_M	0.910	1.00
89_A	182_S	0.907	1.00
188_L	198_I	0.906	1.00
31_T	47_L	0.902	1.00
13_F	207_A	0.900	1.00
106_Y	110_I	0.898	1.00
44_S	187_Y	0.897	1.00
9_S	203_V	0.893	1.00
71_L	175_L	0.883	1.00
91_I	95_S	0.880	1.00
59_V	177_T	0.876	1.00
73_F	130_K	0.874	1.00
210_A	213_L	0.868	1.00
62_N	216_I	0.865	1.00
133_G	161_Q	0.864	1.00
194_N	200_Y	0.862	1.00
87_V	142_V	0.860	1.00
116_Q	233_M	0.856	1.00
176_R	179_E	0.854	1.00
165_K	228_F	0.853	1.00
52_M	184_F	0.853	1.00
40_N	105_V	0.853	1.00
103_V	106_Y	0.842	1.00
167_E	170_F	0.839	1.00
60_I	64_K	0.837	1.00
89_A	183_T	0.833	1.00
73_F	134_Y	0.830	1.00
74_T	175_L	0.828	1.00
119_G	122_K	0.823	1.00
34_L	204_T	0.816	1.00
89_A	187_Y	0.816	1.00
16_S	20_G	0.816	1.00
63_F	66_Y	0.810	1.00
203_V	206_P	0.808	1.00
224_H	230_R	0.804	1.00
87_V	91_I	0.801	1.00
116_Q	232_V	0.801	1.00
51_E	180_S	0.801	1.00
74_T	158_I	0.783	1.00
92_L	187_Y	0.783	1.00
81_V	175_L	0.781	1.00
15_A	30_A	0.780	1.00
185_G	188_L	0.772	1.00
19_T	27_S	0.772	1.00
38_Y	200_Y	0.771	1.00
88_G	142_V	0.769	1.00
96_A	146_V	0.763	1.00
83_I	87_V	0.760	1.00
61_L	65_N	0.755	1.00
140_V	154_L	0.753	1.00
22_F	231_L	0.750	1.00
144_Y	179_E	0.748	1.00
229_R	233_M	0.745	1.00
30_A	204_T	0.745	1.00
35_L	43_I	0.743	1.00
118_A	122_K	0.741	1.00
184_F	201_A	0.740	1.00
121_R	125_G	0.738	1.00
9_S	206_P	0.736	1.00
61_L	64_K	0.732	1.00
42_Q	46_I	0.730	1.00
159_N	168_F	0.729	1.00
193_F	198_I	0.723	1.00
50_I	208_V	0.721	1.00
38_Y	42_Q	0.718	1.00
85_S	182_S	0.718	1.00
51_E	150_S	0.716	1.00
7_Y	37_R	0.715	1.00
243_G	247_N	0.713	1.00
96_A	100_L	0.712	1.00
8_L	200_Y	0.708	1.00
31_T	43_I	0.707	1.00
113_I	117_A	0.703	1.00
148_T	172_I	0.702	1.00
218_M	221_V	0.700	1.00
198_I	202_T	0.700	1.00
84_G	142_V	0.696	1.00
231_L	234_S	0.693	1.00
47_L	51_E	0.693	1.00
156_L	160_N	0.688	1.00
28_S	109_L	0.687	1.00
137_T	154_L	0.685	1.00
163_L	167_E	0.685	1.00
177_T	181_V	0.683	1.00
67_S	72_V	0.683	1.00
57_T	61_L	0.682	1.00
8_L	203_V	0.681	1.00
93_K	190_L	0.680	1.00
13_F	17_I	0.678	1.00
169_K	219_G	0.677	1.00
48_N	148_T	0.675	1.00
90_Y	190_L	0.673	1.00
172_I	176_R	0.671	1.00
40_N	101_F	0.671	1.00
9_S	13_F	0.668	1.00
149_I	152_P	0.666	1.00
174_V	178_V	0.665	1.00
216_I	220_N	0.664	1.00
201_A	205_S	0.664	1.00
17_I	211_G	0.664	1.00
85_S	89_A	0.660	1.00
130_K	134_Y	0.658	1.00
165_K	169_K	0.656	1.00
226_E	229_R	0.655	1.00
115_L	119_G	0.655	1.00
36_I	245_S	0.650	1.00
45_P	193_F	0.650	1.00
20_G	216_I	0.648	1.00
20_G	212_M	0.647	1.00
61_L	213_L	0.644	1.00
65_N	217_F	0.642	1.00
222_I	227_D	0.641	1.00
106_Y	246_F	0.637	1.00
233_M	240_S	0.637	1.00
61_L	217_F	0.634	1.00
111_P	114_L	0.633	1.00
55_N	150_S	0.632	1.00
110_I	113_I	0.627	1.00
66_Y	71_L	0.625	1.00
137_T	141_G	0.624	1.00
119_G	229_R	0.620	1.00
123_E	126_L	0.619	1.00
231_L	235_F	0.618	1.00
157_I	161_Q	0.617	1.00
155_A	168_F	0.613	1.00
66_Y	174_V	0.612	1.00
33_L	37_R	0.611	1.00
19_T	176_R	0.611	1.00
55_N	177_T	0.610	1.00
16_S	26_F	0.610	1.00
30_A	47_L	0.609	1.00
154_L	158_I	0.608	1.00
55_N	180_S	0.606	1.00
210_A	214_I	0.603	1.00
208_V	212_M	0.596	1.00
140_V	143_L	0.593	1.00
112_L	152_P	0.591	1.00
140_V	150_S	0.591	1.00
199_Y	202_T	0.590	1.00
88_G	92_L	0.585	1.00
132_I	136_V	0.582	1.00
50_I	204_T	0.580	1.00
110_I	244_L	0.579	1.00
67_S	171_A	0.578	1.00
31_T	44_S	0.576	1.00
150_S	154_L	0.574	1.00
230_R	233_M	0.574	1.00
26_F	47_L	0.574	1.00
194_N	198_I	0.573	1.00
144_Y	149_I	0.570	1.00
244_L	247_N	0.566	1.00
30_A	34_L	0.566	1.00
59_V	68_I	0.565	0.99
56_L	59_V	0.564	0.99
24_Y	232_V	0.563	0.99
203_V	207_A	0.563	0.99
232_V	239_I	0.562	0.99
54_Q	208_V	0.561	0.99
158_I	163_L	0.557	0.99
52_M	56_L	0.556	0.99
41_K	44_S	0.554	0.99
113_I	116_Q	0.554	0.99
19_T	51_E	0.553	0.99
212_M	216_I	0.552	0.99
157_I	160_N	0.552	0.99
214_I	218_M	0.551	0.99
154_L	157_I	0.550	0.99
74_T	137_T	0.550	0.99
51_E	144_Y	0.548	0.99
38_Y	43_I	0.546	0.99
38_Y	194_N	0.546	0.99
71_L	75_I	0.545	0.99
109_L	239_I	0.544	0.99
55_N	148_T	0.544	0.99
224_H	228_F	0.543	0.99
40_N	44_S	0.541	0.99
21_A	218_M	0.540	0.99
20_G	58_N	0.539	0.99
183_T	186_A	0.537	0.99
192_I	199_Y	0.536	0.99
199_Y	206_P	0.536	0.99
198_I	201_A	0.535	0.99
53_F	56_L	0.535	0.99
112_L	116_Q	0.534	0.99
152_P	158_I	0.532	0.99
242_Y	245_S	0.530	0.99
48_N	184_F	0.529	0.99
113_I	239_I	0.527	0.99
53_F	209_I	0.526	0.99
41_K	92_L	0.522	0.99
150_S	176_R	0.521	0.99
13_F	16_S	0.520	0.99
55_N	176_R	0.520	0.99
84_G	87_V	0.518	0.99
44_S	92_L	0.517	0.99
7_Y	11_I	0.516	0.99
38_Y	46_I	0.516	0.99
187_Y	192_I	0.513	0.99
54_Q	148_T	0.511	0.99
83_I	138_F	0.510	0.99
16_S	208_V	0.506	0.99
180_S	183_T	0.505	0.99
159_N	165_K	0.505	0.99
21_A	215_G	0.503	0.99
44_S	148_T	0.501	0.99
222_I	228_F	0.500	0.99

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3llqA	3	0.6725	34.7	0.936	Contact Map
2f2bA	4	0.6831	23.4	0.941	Contact Map
3o7qA	1	0.8592	22.8	0.941	Contact Map
2gfpA	2	0.8486	22.5	0.942	Contact Map
4bwzA	2	0.7746	17.5	0.944	Contact Map
1pw4A	1	0.8768	14.8	0.946	Contact Map
4cz8A	2	0.7817	14.3	0.947	Contact Map
3cn5A	3	0.6796	14	0.947	Contact Map
3wdoA	1	0.8556	11	0.949	Contact Map
2cfqA	1	0.4472	10.6	0.95	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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