Q97UQ8 - SULSO - GREMLIN Contacts

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Q97UQ8 - Second ORF in transposon ISC1904

UniProt:	Q97UQ8	Sequences: MLKNLRIRKFEPEEEYVYFTYSIKNDKREKSKELIREYTTLLQKAIDYLWNLTKIQVRKKNGNYKITLPKKREVYKPLREELEKINHLASHYVDKAINDAFSILKSWRKRAIKGRASIEKPRVKKAYVRIKTTLRKVVGESVRITVRPHEYITFSWSKSWFSRRVRELELGEPIIKEEKVYLPFRYKLPWATPVDFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRGRELMRKYSHRERNRVLDFVHKFVNTLLDLYPMTFFAVEKLNKESMFKDANDSLSRKISRTVWGSIHRVLKYKAPLYGSFVKEVNPRFTSRSCPRCGFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIREIARVWVGVIPLMGRRGMNGFPRDFGEAQGLRIDIEYHEIP	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	424 (344)
Sequences:	4389 (2821)
Seq/√Len:	152.1

ECOLI - INSQ
HALSA - Q9HSW9 Q9HHJ9 Q9HM87 Q9HSW7 Q9HHG8 Q9HSZ6 Q9HSD4 Q9HHU6 Q9HSY4
SULSO - Q97UQ8 Q97Z61 Q97U68 Q97YI5 Q97YW9 Q97U51 Q97XQ0 Q97YU1 Q97XB6 Q97Y48 Q97XP1 Q97XQ8 Q97YI2 Q97V47 Q97V25 Q97Y44 Q97WZ3 Q97X39 Q97Y67 Q97YW4 Q7LXH2 Q97XA4 Q97WY6 Q97V53 Q7LXD9 Q7LXH6 Y2105 Q7LX59 Q97YF7 Q97U09

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
264_D	268_K	5.305	1.00
288_N	291_S	4.510	1.00
340_P	367_N	4.215	1.00
320_A	327_V	3.732	1.00
284_V	316_L	3.018	1.00
313_H	329_E	3.012	1.00
206_L	312_I	3.009	1.00
19_F	322_L	3.005	1.00
347_R	350_G	2.948	1.00
311_S	315_V	2.827	1.00
271_N	275_D	2.750	1.00
311_S	314_R	2.495	1.00
334_F	337_R	2.457	1.00
338_S	345_V	2.442	1.00
173_P	182_L	2.432	1.00
337_R	346_S	2.428	1.00
317_K	329_E	2.379	1.00
330_V	372_I	2.369	1.00
312_I	316_L	2.345	1.00
274_L	320_A	2.305	1.00
226_K	302_R	2.285	1.00
293_F	305_S	2.189	1.00
282_F	320_A	2.132	1.00
171_G	187_K	2.126	1.00
232_K	247_G	2.095	1.00
225_L	261_R	2.091	1.00
284_V	327_V	2.077	1.00
267_H	315_V	2.050	1.00
354_E	361_K	2.038	1.00
54_K	120_K	2.023	1.00
335_T	372_I	2.023	1.00
342_C	358_C	1.996	1.00
267_H	318_Y	1.989	1.00
36_R	180_V	1.978	1.00
49_L	97_I	1.976	1.00
263_L	311_S	1.921	1.00
298_D	301_S	1.918	1.00
28_R	31_S	1.915	1.00
270_V	315_V	1.887	1.00
206_L	265_F	1.883	1.00
51_N	55_I	1.867	1.00
37_E	87_H	1.861	1.00
278_P	325_S	1.853	1.00
317_K	321_P	1.841	1.00
247_G	251_M	1.840	1.00
266_V	315_V	1.821	1.00
50_W	100_A	1.809	1.00
341_R	360_F	1.806	1.00
342_C	355_C	1.790	1.00
351_K	364_R	1.788	1.00
242_K	245_K	1.772	1.00
339_C	342_C	1.772	1.00
339_C	358_C	1.758	1.00
353_F	362_L	1.748	1.00
160_W	165_V	1.722	1.00
284_V	329_E	1.720	1.00
355_C	358_C	1.716	1.00
267_H	319_K	1.693	1.00
266_V	311_S	1.669	1.00
346_S	350_G	1.665	1.00
232_K	235_K	1.664	1.00
245_K	248_R	1.659	1.00
267_H	270_V	1.630	1.00
44_K	84_K	1.603	1.00
33_E	37_E	1.572	1.00
286_K	329_E	1.571	1.00
68_L	72_R	1.562	1.00
35_I	180_V	1.543	1.00
339_C	355_C	1.538	1.00
199_I	207_D	1.537	1.00
208_A	366_L	1.534	1.00
284_V	313_H	1.513	1.00
20_T	183_P	1.510	1.00
344_F	356_E	1.500	1.00
352_T	361_K	1.495	1.00
101_F	105_K	1.491	1.00
32_K	36_R	1.486	1.00
196_F	373_Y	1.481	1.00
49_L	100_A	1.449	1.00
353_F	360_F	1.440	1.00
375_K	380_P	1.429	1.00
111_A	118_I	1.429	1.00
142_V	145_T	1.424	1.00
205_T	208_A	1.423	1.00
370_L	374_L	1.419	1.00
209_Y	213_K	1.400	1.00
106_S	118_I	1.398	1.00
110_R	118_I	1.394	1.00
317_K	327_V	1.384	1.00
285_E	332_P	1.382	1.00
39_T	180_V	1.382	1.00
236_V	248_R	1.376	1.00
270_V	318_Y	1.375	1.00
295_D	301_S	1.366	1.00
270_V	319_K	1.358	1.00
197_L	209_Y	1.331	1.00
256_H	260_N	1.314	1.00
348_K	351_K	1.302	1.00
304_I	309_W	1.278	1.00
91_H	131_K	1.265	1.00
197_L	277_Y	1.261	1.00
177_E	256_H	1.242	1.00
233_R	237_Q	1.239	1.00
69_P	72_R	1.232	1.00
270_V	312_I	1.226	1.00
105_K	109_K	1.220	1.00
99_D	129_R	1.219	1.00
229_M	251_M	1.218	1.00
179_K	256_H	1.217	1.00
274_L	323_Y	1.210	1.00
229_M	255_S	1.202	1.00
196_F	281_F	1.201	1.00
102_S	106_S	1.194	1.00
366_L	370_L	1.190	1.00
225_L	258_E	1.185	1.00
104_L	120_K	1.179	1.00
54_K	58_R	1.179	1.00
340_P	370_L	1.173	1.00
29_E	32_K	1.173	1.00
95_K	98_N	1.169	1.00
142_V	146_V	1.165	1.00
232_K	254_Y	1.160	1.00
198_A	373_Y	1.157	1.00
288_N	332_P	1.154	1.00
79_R	93_V	1.150	1.00
42_L	96_A	1.149	1.00
91_H	133_T	1.137	1.00
347_R	351_K	1.134	1.00
335_T	375_K	1.134	1.00
139_G	142_V	1.133	1.00
338_S	375_K	1.130	1.00
214_F	363_D	1.128	1.00
201_S	309_W	1.121	1.00
330_V	375_K	1.118	0.99
272_T	276_L	1.118	0.99
145_T	151_Y	1.114	0.99
55_I	59_K	1.109	0.99
340_P	362_L	1.108	0.99
62_G	65_K	1.106	0.99
110_R	119_E	1.106	0.99
270_V	316_L	1.104	0.99
203_L	307_T	1.104	0.99
65_K	70_K	1.095	0.99
228_S	232_K	1.091	0.99
263_L	315_V	1.083	0.99
348_K	364_R	1.080	0.99
201_S	313_H	1.079	0.99
128_V	132_T	1.078	0.99
315_V	318_Y	1.074	0.99
46_I	96_A	1.073	0.99
259_R	263_L	1.069	0.99
193_P	278_P	1.063	0.99
266_V	312_I	1.061	0.99
347_R	364_R	1.055	0.99
66_I	70_K	1.055	0.99
27_K	31_S	1.055	0.99
362_L	370_L	1.051	0.99
236_V	251_M	1.051	0.99
21_Y	186_Y	1.038	0.99
319_K	322_L	1.038	0.99
314_R	317_K	1.037	0.99
106_S	111_A	1.035	0.99
199_I	209_Y	1.035	0.99
205_T	216_T	1.027	0.99
271_N	323_Y	1.025	0.99
274_L	325_S	1.023	0.99
275_D	323_Y	1.020	0.99
143_R	151_Y	1.015	0.99
89_A	92_Y	1.013	0.99
24_K	179_K	1.007	0.99
138_V	141_S	1.006	0.99
54_K	104_L	1.000	0.99
286_K	332_P	0.997	0.99
266_V	270_V	0.995	0.99
289_K	310_G	0.990	0.99
71_K	98_N	0.990	0.99
283_A	372_I	0.989	0.99
281_F	326_F	0.989	0.99
240_A	244_T	0.989	0.99
207_D	269_F	0.981	0.99
233_R	251_M	0.979	0.99
32_K	180_V	0.977	0.99
362_L	366_L	0.976	0.99
156_W	159_S	0.974	0.99
244_T	248_R	0.972	0.99
337_R	345_V	0.972	0.99
213_K	272_T	0.972	0.99
197_L	210_D	0.971	0.99
351_K	363_D	0.969	0.99
274_L	282_F	0.967	0.99
295_D	299_S	0.964	0.98
75_Y	78_L	0.964	0.98
208_A	214_F	0.954	0.98
107_W	120_K	0.953	0.98
106_S	109_K	0.949	0.98
27_K	35_I	0.941	0.98
232_K	251_M	0.938	0.98
70_K	73_E	0.937	0.98
229_M	258_E	0.936	0.98
136_K	139_G	0.934	0.98
129_R	172_E	0.931	0.98
295_D	298_D	0.926	0.98
95_K	129_R	0.926	0.98
340_P	360_F	0.919	0.98
341_R	370_L	0.916	0.98
50_W	104_L	0.914	0.98
94_D	98_N	0.910	0.98
344_F	357_R	0.907	0.98
44_K	48_Y	0.905	0.98
346_S	353_F	0.904	0.98
352_T	362_L	0.903	0.98
74_V	97_I	0.903	0.98
46_I	100_A	0.902	0.98
250_L	253_K	0.901	0.98
340_P	374_L	0.900	0.98
45_A	96_A	0.896	0.98
366_L	369_S	0.895	0.98
334_F	375_K	0.894	0.98
106_S	110_R	0.892	0.97
368_A	371_N	0.892	0.97
40_T	43_Q	0.891	0.97
230_E	233_R	0.889	0.97
67_T	98_N	0.886	0.97
128_V	131_K	0.886	0.97
18_Y	187_K	0.884	0.97
196_F	283_A	0.883	0.97
18_Y	185_R	0.883	0.97
66_I	69_P	0.882	0.97
232_K	250_L	0.879	0.97
281_F	328_K	0.878	0.97
273_L	282_F	0.876	0.97
91_H	95_K	0.871	0.97
53_T	101_F	0.870	0.97
332_P	335_T	0.869	0.97
218_S	307_T	0.869	0.97
46_I	103_I	0.865	0.97
76_K	80_E	0.864	0.97
95_K	99_D	0.860	0.97
346_S	364_R	0.859	0.97
254_Y	258_E	0.857	0.97
320_A	325_S	0.853	0.97
350_G	364_R	0.851	0.97
195_D	211_G	0.851	0.97
229_M	237_Q	0.850	0.97
266_V	308_V	0.850	0.97
175_I	182_L	0.850	0.97
257_R	261_R	0.849	0.97
73_E	76_K	0.849	0.97
176_K	179_K	0.847	0.97
107_W	121_P	0.846	0.97
48_Y	52_L	0.845	0.97
230_E	234_A	0.842	0.96
353_F	364_R	0.841	0.96
154_F	157_S	0.841	0.96
203_L	303_K	0.839	0.96
221_Q	225_L	0.833	0.96
236_V	247_G	0.831	0.96
65_K	74_V	0.829	0.96
367_N	371_N	0.826	0.96
91_H	132_T	0.823	0.96
99_D	174_I	0.820	0.96
174_I	183_P	0.815	0.96
370_L	373_Y	0.813	0.96
289_K	309_W	0.813	0.96
57_V	104_L	0.809	0.96
205_T	214_F	0.801	0.95
149_H	152_I	0.801	0.95
271_N	319_K	0.800	0.95
67_T	71_K	0.799	0.95
221_Q	261_R	0.798	0.95
169_E	187_K	0.792	0.95
41_L	84_K	0.791	0.95
344_F	353_F	0.790	0.95
41_L	88_L	0.789	0.95
24_K	181_Y	0.784	0.95
201_S	207_D	0.783	0.95
85_I	93_V	0.783	0.95
79_R	85_I	0.780	0.95
269_F	272_T	0.780	0.95
293_F	301_S	0.778	0.94
136_K	145_T	0.776	0.94
340_P	368_A	0.775	0.94
134_L	147_R	0.774	0.94
63_N	66_I	0.772	0.94
23_I	158_K	0.769	0.94
23_I	182_L	0.769	0.94
265_F	269_F	0.769	0.94
248_R	252_R	0.766	0.94
338_S	371_N	0.766	0.94
70_K	75_Y	0.765	0.94
144_I	182_L	0.764	0.94
214_F	366_L	0.763	0.94
197_L	282_F	0.763	0.94
218_S	221_Q	0.762	0.94
53_T	104_L	0.759	0.94
26_D	155_S	0.755	0.94
77_P	81_E	0.753	0.93
233_R	255_S	0.753	0.93
34_L	38_Y	0.752	0.93
300_L	304_I	0.751	0.93
273_L	277_Y	0.748	0.93
192_T	195_D	0.744	0.93
287_L	332_P	0.744	0.93
283_A	373_Y	0.743	0.93
229_M	233_R	0.741	0.93
291_S	332_P	0.741	0.93
25_N	182_L	0.741	0.93
231_V	235_K	0.740	0.93
103_I	121_P	0.740	0.93
291_S	304_I	0.734	0.93
95_K	131_K	0.731	0.92
225_L	257_R	0.722	0.92
97_I	101_F	0.721	0.92
286_K	313_H	0.721	0.92
91_H	94_D	0.720	0.92
51_N	123_V	0.720	0.92
44_K	82_L	0.717	0.92
235_K	247_G	0.716	0.92
283_A	375_K	0.713	0.91
133_T	136_K	0.711	0.91
230_E	237_Q	0.709	0.91
200_D	365_Q	0.708	0.91
104_L	107_W	0.707	0.91
283_A	379_F	0.707	0.91
40_T	44_K	0.706	0.91
241_S	244_T	0.704	0.91
72_R	76_K	0.702	0.91
282_F	316_L	0.702	0.91
258_E	306_R	0.698	0.91
206_L	307_T	0.695	0.90
172_E	185_R	0.693	0.90
52_L	56_Q	0.693	0.90
204_Y	208_A	0.692	0.90
29_E	33_E	0.691	0.90
75_Y	94_D	0.690	0.90
207_D	312_I	0.689	0.90
100_A	104_L	0.688	0.90
37_E	41_L	0.688	0.90
69_P	73_E	0.685	0.90
88_L	91_H	0.685	0.90
310_G	314_R	0.685	0.90
334_F	338_S	0.684	0.90
131_K	134_L	0.681	0.90
190_W	193_P	0.677	0.89
215_V	265_F	0.676	0.89
263_L	267_H	0.675	0.89
356_E	364_R	0.675	0.89
41_L	44_K	0.672	0.89
283_A	328_K	0.671	0.89
30_K	33_E	0.670	0.89
76_K	79_R	0.666	0.88
129_R	174_I	0.666	0.88
202_N	208_A	0.666	0.88
104_L	108_R	0.665	0.88
262_V	306_R	0.663	0.88
334_F	345_V	0.662	0.88
69_P	75_Y	0.661	0.88
190_W	325_S	0.660	0.88
52_L	74_V	0.660	0.88
237_Q	251_M	0.660	0.88
38_Y	42_L	0.658	0.88
206_L	270_V	0.657	0.88
23_I	165_V	0.654	0.88
179_K	260_N	0.651	0.87
316_L	327_V	0.650	0.87
91_H	134_L	0.650	0.87
295_D	304_I	0.647	0.87
225_L	229_M	0.645	0.87
313_H	317_K	0.645	0.87
96_A	100_A	0.644	0.87
17_V	187_K	0.644	0.87
346_S	351_K	0.643	0.87
235_K	250_L	0.641	0.87
298_D	305_S	0.640	0.86
35_I	39_T	0.638	0.86
338_S	343_G	0.637	0.86
138_V	143_R	0.634	0.86
97_I	100_A	0.633	0.86
25_N	154_F	0.631	0.86
131_K	135_R	0.629	0.86
277_Y	282_F	0.629	0.86
71_K	74_V	0.627	0.85
20_T	185_R	0.625	0.85
195_D	277_Y	0.624	0.85
49_L	74_V	0.623	0.85
225_L	254_Y	0.622	0.85
350_G	353_F	0.621	0.85
243_H	246_R	0.619	0.85
65_K	71_K	0.616	0.84
144_I	147_R	0.615	0.84
68_L	71_K	0.613	0.84
255_S	262_V	0.612	0.84
154_F	158_K	0.611	0.84
92_Y	128_V	0.608	0.84
22_S	183_P	0.608	0.84
107_W	110_R	0.606	0.83
146_V	175_I	0.606	0.83
52_L	78_L	0.605	0.83
235_K	238_S	0.605	0.83
134_L	137_V	0.605	0.83
38_Y	89_A	0.604	0.83
206_L	266_V	0.604	0.83
203_L	294_K	0.604	0.83
348_K	363_D	0.604	0.83
48_Y	78_L	0.602	0.83
93_V	96_A	0.602	0.83
236_V	244_T	0.601	0.83
71_K	75_Y	0.601	0.83
142_V	147_R	0.601	0.83
52_L	55_I	0.601	0.83
362_L	367_N	0.600	0.83
21_Y	158_K	0.598	0.83
341_R	362_L	0.597	0.82
215_V	272_T	0.595	0.82
255_S	259_R	0.593	0.82
52_L	73_E	0.590	0.82
245_K	249_E	0.590	0.82
209_Y	215_V	0.588	0.81
244_T	251_M	0.587	0.81
372_I	375_K	0.587	0.81
67_T	70_K	0.586	0.81
109_K	118_I	0.579	0.80
338_S	353_F	0.577	0.80
34_L	37_E	0.572	0.80
232_K	236_V	0.572	0.80
55_I	58_R	0.572	0.80
318_Y	322_L	0.571	0.80
289_K	292_M	0.569	0.79
128_V	134_L	0.569	0.79
233_R	238_S	0.569	0.79
210_D	366_L	0.568	0.79
90_S	133_T	0.567	0.79
275_D	281_F	0.567	0.79
334_F	353_F	0.565	0.79
66_I	71_K	0.564	0.79
350_G	356_E	0.563	0.79
222_L	225_L	0.562	0.79
51_N	54_K	0.561	0.78
223_Y	226_K	0.559	0.78
75_Y	79_R	0.559	0.78
215_V	269_F	0.557	0.78
320_A	323_Y	0.557	0.78
70_K	74_V	0.556	0.78
223_Y	302_R	0.555	0.78
76_K	97_I	0.554	0.78
234_A	238_S	0.554	0.78
260_N	263_L	0.551	0.77
203_L	293_F	0.551	0.77
244_T	247_G	0.551	0.77
269_F	312_I	0.550	0.77
333_R	337_R	0.549	0.77
127_Y	130_I	0.549	0.77
331_N	334_F	0.548	0.77
27_K	152_I	0.545	0.76
315_V	319_K	0.545	0.76
230_E	298_D	0.545	0.76
42_L	128_V	0.544	0.76
45_A	93_V	0.544	0.76
223_Y	303_K	0.543	0.76
248_R	251_M	0.541	0.76
200_D	336_S	0.540	0.76
283_A	378_G	0.539	0.76
181_Y	260_N	0.539	0.76
38_Y	88_L	0.539	0.76
291_S	294_K	0.539	0.76
266_V	269_F	0.539	0.76
154_F	159_S	0.538	0.75
127_Y	131_K	0.538	0.75
92_Y	130_I	0.536	0.75
74_V	94_D	0.535	0.75
136_K	143_R	0.533	0.75
204_Y	351_K	0.532	0.75
310_G	313_H	0.532	0.75
27_K	32_K	0.530	0.74
129_R	132_T	0.529	0.74
300_L	303_K	0.528	0.74
18_Y	171_G	0.527	0.74
48_Y	84_K	0.527	0.74
73_E	77_P	0.527	0.74
348_K	352_T	0.527	0.74
271_N	274_L	0.527	0.74
289_K	304_I	0.522	0.73
39_T	128_V	0.520	0.73
181_Y	256_H	0.520	0.73
37_E	88_L	0.519	0.73
201_S	312_I	0.519	0.73
68_L	74_V	0.518	0.73
293_F	298_D	0.518	0.73
146_V	149_H	0.518	0.73
66_I	75_Y	0.517	0.73
130_I	135_R	0.516	0.72
223_Y	227_Y	0.516	0.72
108_R	111_A	0.514	0.72
336_S	346_S	0.514	0.72
230_E	238_S	0.513	0.72
88_L	93_V	0.513	0.72
66_I	72_R	0.513	0.72
37_E	40_T	0.513	0.72
74_V	78_L	0.512	0.72
219_L	307_T	0.511	0.72
41_L	85_I	0.511	0.72
71_K	97_I	0.511	0.72
57_V	108_R	0.510	0.72
354_E	360_F	0.509	0.71
268_K	271_N	0.509	0.71
143_R	153_T	0.508	0.71
22_S	263_L	0.508	0.71
76_K	83_E	0.507	0.71
58_R	62_G	0.506	0.71
38_Y	130_I	0.505	0.71
38_Y	41_L	0.504	0.71
196_F	379_F	0.503	0.71
342_C	345_V	0.501	0.70
176_K	256_H	0.501	0.70
127_Y	132_T	0.500	0.70
53_T	71_K	0.500	0.70

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1nu0A	1	0.3066	94.8	0.935	Contact Map
1vhxA	2	0.3231	94.3	0.936	Contact Map
1iv0A	1	0.2288	91.4	0.942	Contact Map
2k4xA	1	0.125	86.1	0.948	Contact Map
3j20Y	1	0.1132	85.4	0.948	Contact Map
1qypA	1	0.1014	84.3	0.949	Contact Map
4aybP	1	0.0943	81.7	0.95	Contact Map
1vd4A	1	0.125	75.6	0.953	Contact Map
1ffkW	1	0.1651	73.2	0.954	Contact Map
3j3w0	1	0.1297	72.2	0.954	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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