Q97UM3 - SULSO - GREMLIN Contacts

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Q97UM3 - Epimerase, putative

UniProt:	Q97UM3	Sequences: MKILTFGGTGFVGSNFVRYAISKGHDVLVYARSMNDYTKALQNIGANIVFSYDNYLKDIDCLVYFIGAMWTKDPREFEYLQVQFPTEIGRKFFQVNSGKFVYISSIGVSENIESKERPIAEESPHGEGLKPNTLHGITKLEGERNISKFPNYVILRFPIVYGPYSRILMWRAFMWLVSHGIGLKNDNMFSVVSTRNTSKAIELACKYKRSDYFYITDKEPANLTSLFSDAVKAINREIRSWLPFSVKLIEPFKSSHEMLKMAYDVLSRELVYSYKKAERELNYIPEDVRIETFKEMAKYYKII	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	303 (270)
Sequences:	62606 (44375)
Seq/√Len:	2700.6

ECOLI - GM4D GALE RMLB1 RMLB2 YBJS HLDD YFCH FCL RMLD YEEZ QOR2 YBJT ARNA YRAR
BACSU - YTCB GALE YFHF RMLB YFNG SPSK YTBQ YESF YWNB YHFK YHEG
HALSA - Q9HSV1 Q9HSU9 Q9HSE7 Q9HRD1 Q9HML4 Q9HS50 Q9HPF1 Q9HSQ3 Q9HNV3 Q9HR86
SULSO - Q97UM3 Q97VN5 Q980W1 Q7LXG5 Q7LXE8 Q97XG1 Q7LX44 Q97XJ9 Q7LXE6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
8_G	29_V	3.088	1.00
229_D	290_I	2.976	1.00
19_Y	199_K	2.617	1.00
200_A	215_I	2.540	1.00
79_Y	83_Q	2.492	1.00
211_D	276_K	2.453	1.00
61_C	205_C	2.415	1.00
103_I	201_I	2.323	1.00
53_D	94_Q	2.307	1.00
288_V	291_E	2.273	1.00
19_Y	202_E	2.253	1.00
213_F	280_E	2.175	1.00
73_D	76_E	2.136	1.00
19_Y	23_K	2.123	1.00
30_Y	44_I	2.120	1.00
64_Y	102_Y	2.115	1.00
233_A	294_K	2.066	1.00
232_K	290_I	2.061	1.00
99_K	151_N	2.038	1.00
120_A	123_S	2.032	1.00
199_K	286_E	1.976	1.00
48_I	51_S	1.944	1.00
63_V	205_C	1.937	1.00
224_T	228_S	1.933	1.00
6_F	64_Y	1.896	1.00
44_I	51_S	1.863	1.00
229_D	232_K	1.854	1.00
157_F	201_I	1.854	1.00
195_R	295_E	1.836	1.00
86_T	145_N	1.828	1.00
278_E	284_I	1.815	1.00
222_N	225_S	1.769	1.00
218_K	278_E	1.766	1.00
5_T	17_V	1.759	1.00
155_L	204_A	1.741	1.00
199_K	203_L	1.728	1.00
146_I	152_Y	1.708	1.00
161_Y	292_T	1.705	1.00
23_K	202_E	1.701	1.00
28_L	59_I	1.694	1.00
140_L	144_R	1.674	1.00
49_V	90_R	1.663	1.00
78_E	141_E	1.634	1.00
53_D	91_K	1.627	1.00
202_E	206_K	1.613	1.00
23_K	206_K	1.592	1.00
86_T	90_R	1.581	1.00
175_W	179_H	1.577	1.00
83_Q	86_T	1.570	1.00
193_S	295_E	1.551	1.00
16_F	198_S	1.543	1.00
274_Y	278_E	1.518	1.00
47_N	79_Y	1.515	1.00
19_Y	22_S	1.499	1.00
229_D	233_A	1.494	1.00
60_D	98_G	1.486	1.00
16_F	63_V	1.484	1.00
85_P	142_G	1.473	1.00
99_K	153_V	1.469	1.00
277_A	281_L	1.456	1.00
16_F	65_F	1.446	1.00
75_R	79_Y	1.442	1.00
19_Y	198_S	1.439	1.00
49_V	87_E	1.429	1.00
51_S	54_N	1.425	1.00
28_L	42_Q	1.412	1.00
28_L	40_A	1.409	1.00
135_H	139_K	1.408	1.00
17_V	29_V	1.394	1.00
62_L	92_F	1.393	1.00
203_L	207_Y	1.388	1.00
129_L	140_L	1.381	1.00
121_E	276_K	1.378	1.00
176_L	181_I	1.372	1.00
104_S	108_V	1.371	1.00
54_N	58_D	1.364	1.00
12_V	160_V	1.349	1.00
65_F	103_I	1.345	1.00
157_F	197_T	1.340	1.00
3_I	25_H	1.333	1.00
89_G	149_F	1.330	1.00
188_M	220_P	1.325	1.00
103_I	157_F	1.324	1.00
57_K	95_V	1.293	1.00
53_D	95_V	1.288	1.00
49_V	53_D	1.284	1.00
25_H	206_K	1.275	1.00
193_S	292_T	1.275	1.00
225_S	228_S	1.270	1.00
26_D	40_A	1.269	1.00
147_S	154_I	1.267	1.00
194_T	198_S	1.259	1.00
8_G	17_V	1.256	1.00
155_L	201_I	1.253	1.00
158_P	272_Y	1.251	1.00
63_V	201_I	1.220	1.00
4_L	59_I	1.218	1.00
144_R	147_S	1.212	1.00
18_R	164_Y	1.202	1.00
20_A	202_E	1.197	1.00
192_V	197_T	1.194	1.00
137_I	141_E	1.194	1.00
291_E	294_K	1.188	1.00
109_S	214_Y	1.185	1.00
90_R	93_F	1.182	1.00
145_N	148_K	1.180	1.00
239_R	243_P	1.177	1.00
64_Y	85_P	1.175	1.00
102_Y	142_G	1.161	1.00
120_A	273_S	1.157	1.00
46_A	84_F	1.156	1.00
81_Q	138_T	1.149	1.00
243_P	246_V	1.149	1.00
276_K	280_E	1.148	1.00
4_L	30_Y	1.130	1.00
169_M	173_F	1.130	1.00
33_S	41_L	1.127	1.00
186_D	224_T	1.124	1.00
291_E	295_E	1.123	1.00
61_C	99_K	1.118	1.00
54_N	57_K	1.113	1.00
19_Y	195_R	1.100	1.00
211_D	280_E	1.090	1.00
119_I	123_S	1.088	1.00
156_R	212_Y	1.082	1.00
190_S	218_K	1.081	1.00
102_Y	146_I	1.081	1.00
200_A	277_A	1.079	1.00
274_Y	285_P	1.075	1.00
92_F	100_F	1.059	1.00
147_S	152_Y	1.056	1.00
122_E	276_K	1.055	1.00
101_V	155_L	1.054	1.00
186_D	222_N	1.052	1.00
9_T	31_A	1.048	1.00
88_I	100_F	1.047	1.00
46_A	66_I	1.047	1.00
15_N	195_R	1.040	1.00
40_A	59_I	1.032	1.00
221_A	289_R	1.032	1.00
3_I	20_A	1.029	1.00
290_I	294_K	1.023	1.00
231_V	238_I	1.022	1.00
15_N	194_T	1.021	1.00
147_S	212_Y	1.018	1.00
11_F	162_G	1.016	1.00
231_V	237_E	1.009	1.00
221_A	225_S	1.009	1.00
228_S	231_V	1.008	1.00
18_R	22_S	1.008	1.00
68_A	80_L	1.005	1.00
89_G	100_F	1.004	1.00
172_A	175_W	1.002	1.00
216_T	272_Y	1.001	1.00
90_R	94_Q	1.001	1.00
203_L	281_L	1.000	1.00
91_K	94_Q	0.988	1.00
53_D	57_K	0.983	1.00
199_K	283_Y	0.981	1.00
220_P	271_V	0.972	1.00
92_F	97_S	0.970	1.00
83_Q	87_E	0.968	1.00
102_Y	154_I	0.965	1.00
234_I	297_A	0.962	1.00
233_A	297_A	0.959	1.00
87_E	90_R	0.958	1.00
77_F	138_T	0.954	1.00
91_K	95_V	0.953	1.00
6_F	88_I	0.952	1.00
188_M	222_N	0.951	1.00
220_P	269_E	0.950	1.00
78_E	82_V	0.947	1.00
172_A	176_L	0.947	1.00
52_Y	91_K	0.946	1.00
90_R	149_F	0.943	1.00
3_I	61_C	0.942	1.00
203_L	206_K	0.939	1.00
82_V	141_E	0.937	1.00
78_E	83_Q	0.933	1.00
65_F	201_I	0.931	1.00
100_F	146_I	0.923	1.00
151_N	209_R	0.915	1.00
141_E	144_R	0.912	1.00
33_S	36_D	0.908	1.00
30_Y	55_Y	0.907	1.00
56_L	91_K	0.906	1.00
168_L	171_R	0.898	1.00
153_V	204_A	0.897	1.00
15_N	18_R	0.894	1.00
50_F	54_N	0.890	1.00
114_S	123_S	0.890	1.00
156_R	214_Y	0.890	1.00
99_K	208_K	0.887	1.00
80_L	84_F	0.885	1.00
157_F	160_V	0.883	1.00
55_Y	59_I	0.882	1.00
11_F	194_T	0.882	1.00
56_L	59_I	0.879	1.00
188_M	269_E	0.879	1.00
240_S	243_P	0.877	1.00
213_F	276_K	0.872	1.00
50_F	53_D	0.871	1.00
114_S	120_A	0.870	1.00
160_V	197_T	0.869	1.00
56_L	62_L	0.869	1.00
28_L	58_D	0.867	1.00
89_G	146_I	0.867	1.00
196_N	217_D	0.866	1.00
14_S	164_Y	0.865	1.00
193_S	196_N	0.863	1.00
200_A	283_Y	0.857	1.00
143_E	156_R	0.857	1.00
16_F	20_A	0.856	1.00
233_A	290_I	0.854	1.00
244_F	247_K	0.850	1.00
93_F	149_F	0.850	1.00
175_W	178_S	0.837	1.00
199_K	202_E	0.835	1.00
155_L	215_I	0.835	1.00
154_I	212_Y	0.834	1.00
161_Y	193_S	0.830	1.00
62_L	88_I	0.828	1.00
231_V	236_R	0.827	1.00
15_N	19_Y	0.826	1.00
68_A	76_E	0.825	1.00
30_Y	52_Y	0.820	1.00
87_E	91_K	0.819	1.00
21_I	27_V	0.819	1.00
216_T	274_Y	0.819	1.00
30_Y	51_S	0.817	1.00
243_P	247_K	0.814	1.00
17_V	27_V	0.804	1.00
34_M	43_N	0.802	1.00
6_F	66_I	0.800	1.00
51_S	55_Y	0.799	1.00
56_L	95_V	0.798	1.00
104_S	143_E	0.796	1.00
49_V	94_Q	0.791	1.00
219_E	288_V	0.787	1.00
33_S	43_N	0.786	1.00
176_L	180_G	0.784	1.00
25_H	60_D	0.781	1.00
225_S	229_D	0.778	1.00
218_K	274_Y	0.778	1.00
204_A	281_L	0.776	1.00
160_V	192_V	0.771	1.00
106_I	158_P	0.770	1.00
3_I	63_V	0.765	1.00
85_P	146_I	0.763	1.00
144_R	148_K	0.761	1.00
242_L	245_S	0.759	1.00
48_I	52_Y	0.756	1.00
242_L	247_K	0.754	1.00
47_N	80_L	0.752	1.00
185_N	224_T	0.752	1.00
68_A	77_F	0.751	1.00
42_Q	58_D	0.748	1.00
169_M	172_A	0.737	1.00
141_E	145_N	0.736	1.00
106_I	272_Y	0.734	1.00
76_E	79_Y	0.731	1.00
275_K	278_E	0.730	1.00
89_G	145_N	0.730	1.00
198_S	202_E	0.728	1.00
32_R	45_G	0.725	1.00
3_I	205_C	0.725	1.00
203_L	283_Y	0.721	1.00
241_W	244_F	0.721	1.00
133_T	136_G	0.718	1.00
42_Q	59_I	0.715	1.00
15_N	198_S	0.712	1.00
167_I	171_R	0.712	1.00
177_V	231_V	0.711	1.00
131_P	136_G	0.710	1.00
74_P	137_I	0.709	1.00
233_A	293_F	0.706	1.00
85_P	89_G	0.705	1.00
64_Y	88_I	0.702	1.00
117_R	123_S	0.697	1.00
52_Y	87_E	0.697	1.00
84_F	87_E	0.697	1.00
33_S	38_T	0.691	1.00
31_A	41_L	0.690	1.00
242_L	246_V	0.685	1.00
168_L	172_A	0.684	1.00
98_G	151_N	0.684	1.00
169_M	177_V	0.683	1.00
196_N	199_K	0.682	1.00
145_N	149_F	0.682	1.00
78_E	138_T	0.678	1.00
203_L	282_N	0.676	1.00
45_G	48_I	0.674	1.00
173_F	176_L	0.674	1.00
62_L	100_F	0.671	1.00
16_F	201_I	0.669	1.00
12_V	197_T	0.666	1.00
118_P	273_S	0.662	1.00
134_L	137_I	0.658	1.00
14_S	18_R	0.657	1.00
110_E	130_K	0.657	1.00
176_L	179_H	0.656	1.00
56_L	92_F	0.654	1.00
61_C	101_V	0.651	1.00
25_H	61_C	0.650	1.00
76_E	80_L	0.649	1.00
221_A	226_L	0.648	1.00
142_G	146_I	0.645	1.00
224_T	242_L	0.642	1.00
204_A	277_A	0.642	1.00
278_E	285_P	0.640	1.00
150_P	210_S	0.638	1.00
152_Y	210_S	0.637	1.00
228_S	232_K	0.633	1.00
118_P	271_V	0.630	1.00
113_E	123_S	0.629	1.00
169_M	176_L	0.627	1.00
171_R	177_V	0.627	1.00
241_W	247_K	0.625	1.00
121_E	277_A	0.624	1.00
20_A	205_C	0.623	1.00
180_G	236_R	0.623	1.00
167_I	172_A	0.621	1.00
229_D	289_R	0.620	1.00
64_Y	100_F	0.619	1.00
239_R	242_L	0.618	1.00
78_E	137_I	0.617	1.00
44_I	52_Y	0.615	1.00
163_P	295_E	0.603	1.00
163_P	193_S	0.602	1.00
15_N	164_Y	0.601	1.00
112_I	119_I	0.599	1.00
226_L	289_R	0.596	1.00
113_E	117_R	0.594	1.00
137_I	144_R	0.592	1.00
4_L	44_I	0.592	1.00
267_S	270_L	0.591	1.00
188_M	271_V	0.590	1.00
177_V	180_G	0.590	1.00
42_Q	55_Y	0.588	1.00
4_L	62_L	0.587	1.00
120_A	275_K	0.587	1.00
171_R	176_L	0.587	1.00
108_V	143_E	0.585	1.00
231_V	234_I	0.583	1.00
23_K	203_L	0.582	1.00
190_S	220_P	0.582	1.00
196_N	286_E	0.581	1.00
74_P	134_L	0.575	1.00
92_F	95_V	0.575	1.00
158_P	216_T	0.574	1.00
147_S	150_P	0.574	1.00
101_V	205_C	0.571	1.00
131_P	140_L	0.570	1.00
55_Y	58_D	0.570	1.00
178_S	181_I	0.569	1.00
175_W	181_I	0.568	1.00
138_T	142_G	0.564	1.00
227_F	231_V	0.563	1.00
67_G	81_Q	0.563	1.00
5_T	20_A	0.563	1.00
221_A	229_D	0.562	1.00
17_V	21_I	0.560	1.00
171_R	175_W	0.559	1.00
235_N	238_I	0.555	1.00
134_L	138_T	0.554	1.00
135_H	138_T	0.550	1.00
101_V	204_A	0.548	1.00
24_G	27_V	0.548	1.00
137_I	148_K	0.547	1.00
215_I	277_A	0.545	1.00
239_R	246_V	0.544	1.00
187_N	223_L	0.542	1.00
219_E	290_I	0.540	1.00
101_V	201_I	0.539	1.00
286_E	291_E	0.538	1.00
11_F	15_N	0.537	1.00
16_F	202_E	0.535	1.00
9_T	29_V	0.534	1.00
49_V	91_K	0.534	1.00
190_S	217_D	0.533	1.00
68_A	81_Q	0.532	1.00
82_V	86_T	0.532	1.00
173_F	177_V	0.532	1.00
23_K	199_K	0.531	1.00
4_L	55_Y	0.531	1.00
61_C	98_G	0.529	1.00
9_T	33_S	0.529	1.00
201_I	204_A	0.526	1.00
174_M	297_A	0.525	1.00
277_A	285_P	0.523	1.00
105_S	139_K	0.522	1.00
117_R	269_E	0.519	1.00
153_V	213_F	0.517	1.00
92_F	96_N	0.517	1.00
239_R	247_K	0.515	1.00
209_R	213_F	0.513	1.00
46_A	52_Y	0.512	1.00
86_T	149_F	0.510	1.00
89_G	93_F	0.508	1.00
114_S	117_R	0.503	1.00
155_L	200_A	0.503	1.00
154_I	210_S	0.503	1.00
52_Y	56_L	0.501	1.00
239_R	245_S	0.501	1.00
197_T	215_I	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1sb8A	2	0.9406	100	0.238	Contact Map
3m2pA	2	0.9208	100	0.239	Contact Map
3rufA	2	0.9241	100	0.241	Contact Map
1rkxA	3	0.9142	100	0.243	Contact Map
1rpnA	4	0.9307	100	0.243	Contact Map
2pzmA	2	0.9274	100	0.246	Contact Map
2z1mA	4	0.9802	100	0.249	Contact Map
1n7hA	4	0.934	100	0.249	Contact Map
2c5aA	2	0.934	100	0.256	Contact Map
3slgA	4	0.9406	100	0.256	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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