Q97UH7 - SULSO - GREMLIN Contacts

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Q97UH7 - Uncharacterized protein

UniProt:	Q97UH7	Sequences: MEIELKTLGNYKAILGESPVYDREGNRLFWVDIDGKKVIVNNLDTNEEKYYDTPDLVTSLCLIDYKRIIVTLRHGFHTLNLTTGEITPYLELENNGENRFNDGKCDIRDRYWAGTMNLHIENPKPTASLYKLEGQRLTKVLDKLYRSNGLGWDPDNKLFYLIDTPLRKVFQFDFDKDKGDIYNKRIVMDFKYEPGRPDGMTIDEEGYLWIAHSAGGKVSRWNPKNGEKVSEIKLPVLYVSSVTFGSPEMNQIFITTMSKGGEPLAGRLFTAKMNIKGLQSYKFLT	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	285 (226)
Sequences:	11297 (9220)
Seq/√Len:	613.3

ECOLI - YNCE TOLB 6PGL BAMB
BACSU - YVRE YWHL 6PGL YXAL YWHK YUXL
HALSA - Q9HHP3 Q9HS46
SULSO - Q97UH7 Q97VC7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
149_G	163_D	3.297	1.00
102_D	115_T	3.032	1.00
114_G	130_Y	2.856	1.00
199_G	212_H	2.743	1.00
211_A	221_W	2.733	1.00
193_E	211_A	2.651	1.00
245_G	248_E	2.640	1.00
96_G	114_G	2.638	1.00
241_S	255_T	2.510	1.00
113_A	152_W	2.493	1.00
162_I	172_F	2.321	1.00
222_N	225_N	2.317	1.00
235_P	254_I	2.304	1.00
143_K	162_I	2.299	1.00
80_N	83_T	2.263	1.00
219_S	228_K	2.255	1.00
31_V	41_N	2.210	1.00
30_W	40_V	2.118	1.00
210_I	220_R	2.096	1.00
161_L	209_W	2.088	1.00
210_I	244_F	2.055	1.00
131_K	134_G	2.023	1.00
113_A	129_L	2.007	1.00
161_L	171_Q	1.979	1.00
161_L	202_I	1.900	1.00
104_K	151_G	1.840	1.00
219_S	231_E	1.836	1.00
169_V	211_A	1.800	1.00
221_W	228_K	1.794	1.00
42_N	45_T	1.774	1.00
63_I	105_C	1.754	1.00
143_K	164_T	1.730	1.00
22_D	25_G	1.710	1.00
158_L	171_Q	1.702	1.00
218_V	254_I	1.700	1.00
153_D	202_I	1.699	1.00
127_A	162_I	1.685	1.00
210_I	242_V	1.653	1.00
104_K	150_L	1.565	1.00
113_A	160_Y	1.558	1.00
39_I	48_E	1.533	1.00
50_Y	53_T	1.516	1.00
212_H	239_V	1.506	1.00
245_G	249_M	1.496	1.00
77_H	86_I	1.478	1.00
186_I	189_D	1.471	1.00
106_D	152_W	1.461	1.00
18_S	32_D	1.436	1.00
201_T	243_T	1.434	1.00
193_E	213_S	1.428	1.00
76_F	114_G	1.422	1.00
63_I	67_R	1.416	1.00
217_K	231_E	1.416	1.00
106_D	110_R	1.413	1.00
245_G	251_Q	1.404	1.00
164_T	168_K	1.403	1.00
163_D	197_P	1.403	1.00
96_G	116_M	1.360	1.00
128_S	139_K	1.356	1.00
151_G	201_T	1.348	1.00
170_F	189_D	1.347	1.00
79_L	86_I	1.345	1.00
245_G	253_F	1.340	1.00
173_D	176_K	1.340	1.00
203_D	244_F	1.333	1.00
103_G	114_G	1.325	1.00
115_T	147_S	1.324	1.00
152_W	174_F	1.322	1.00
101_N	117_N	1.321	1.00
201_T	242_V	1.315	1.00
113_A	150_L	1.301	1.00
228_K	231_E	1.288	1.00
30_W	62_L	1.287	1.00
110_R	133_E	1.285	1.00
208_L	229_V	1.273	1.00
118_L	126_T	1.261	1.00
204_E	249_M	1.258	1.00
150_L	162_I	1.253	1.00
198_D	214_A	1.241	1.00
245_G	250_N	1.224	1.00
22_D	62_L	1.206	1.00
143_K	166_L	1.199	1.00
32_D	36_K	1.195	1.00
128_S	136_R	1.189	1.00
244_F	252_I	1.181	1.00
210_I	252_I	1.169	1.00
130_Y	136_R	1.166	1.00
63_I	66_K	1.164	1.00
105_C	112_W	1.162	1.00
203_D	207_Y	1.161	1.00
41_N	48_E	1.148	1.00
151_G	200_M	1.131	1.00
153_D	156_N	1.125	1.00
201_T	244_F	1.122	1.00
96_G	100_F	1.107	1.00
235_P	239_V	1.104	1.00
107_I	152_W	1.097	1.00
203_D	206_G	1.096	1.00
116_M	119_H	1.094	1.00
212_H	242_V	1.085	1.00
148_N	165_P	1.084	1.00
114_G	128_S	1.083	1.00
143_K	147_S	1.076	1.00
211_A	219_S	1.073	1.00
256_T	259_K	1.071	1.00
98_N	117_N	1.069	1.00
168_K	189_D	1.063	1.00
61_C	104_K	1.062	1.00
68_I	71_T	1.059	1.00
163_D	200_M	1.055	1.00
59_S	71_T	1.052	1.00
115_T	150_L	1.049	1.00
153_D	157_K	1.045	1.00
213_S	217_K	1.040	1.00
31_V	39_I	1.040	1.00
161_L	200_M	1.033	1.00
86_I	89_Y	1.032	1.00
164_T	167_R	1.029	1.00
152_W	160_Y	1.025	1.00
33_I	37_K	1.021	1.00
203_D	210_I	1.008	1.00
151_G	202_I	1.002	1.00
154_P	202_I	0.999	1.00
61_C	103_G	0.991	1.00
70_V	105_C	0.986	1.00
61_C	105_C	0.983	1.00
30_W	60_L	0.977	1.00
70_V	79_L	0.977	1.00
22_D	27_R	0.972	1.00
20_V	61_C	0.971	1.00
214_A	238_Y	0.968	1.00
234_L	254_I	0.960	1.00
235_P	256_T	0.951	1.00
154_P	206_G	0.950	1.00
70_V	78_T	0.940	1.00
106_D	111_Y	0.937	1.00
170_F	186_I	0.934	1.00
33_I	36_K	0.934	1.00
200_M	211_A	0.933	1.00
136_R	139_K	0.930	1.00
76_F	103_G	0.926	1.00
55_D	73_R	0.924	1.00
157_K	175_D	0.922	1.00
27_R	42_N	0.920	1.00
64_D	105_C	0.916	1.00
106_D	109_D	0.914	1.00
213_S	219_S	0.907	1.00
202_I	209_W	0.904	1.00
110_R	131_K	0.901	1.00
162_I	170_F	0.899	1.00
217_K	233_K	0.898	1.00
218_V	242_V	0.897	1.00
28_L	32_D	0.891	1.00
33_I	39_I	0.891	1.00
243_T	253_F	0.890	1.00
142_D	172_F	0.889	1.00
193_E	197_P	0.888	1.00
147_S	162_I	0.883	1.00
240_S	257_M	0.881	1.00
69_I	78_T	0.879	1.00
146_R	165_P	0.877	1.00
145_Y	165_P	0.873	1.00
222_N	229_V	0.868	1.00
107_I	156_N	0.851	1.00
204_E	244_F	0.850	1.00
256_T	260_G	0.846	1.00
100_F	116_M	0.844	1.00
255_T	259_K	0.835	1.00
209_W	223_P	0.833	1.00
207_Y	220_R	0.832	1.00
184_K	187_V	0.827	1.00
193_E	221_W	0.826	1.00
198_D	240_S	0.822	1.00
116_M	128_S	0.809	1.00
20_V	62_L	0.808	1.00
169_V	200_M	0.808	1.00
208_L	220_R	0.801	1.00
148_N	198_D	0.796	1.00
186_I	190_F	0.794	1.00
205_E	249_M	0.793	1.00
242_V	254_I	0.791	1.00
42_N	47_E	0.789	1.00
196_R	214_A	0.788	1.00
212_H	216_G	0.784	1.00
37_K	52_D	0.783	1.00
22_D	26_N	0.781	1.00
49_K	52_D	0.776	1.00
32_D	35_G	0.774	1.00
88_P	91_E	0.773	1.00
223_P	226_G	0.773	1.00
102_D	150_L	0.772	1.00
164_T	170_F	0.764	1.00
193_E	215_G	0.761	1.00
192_Y	221_W	0.759	1.00
112_W	132_L	0.758	1.00
62_L	81_L	0.756	1.00
20_V	60_L	0.756	1.00
169_V	197_P	0.752	1.00
192_Y	219_S	0.751	1.00
96_G	128_S	0.747	1.00
101_N	148_N	0.746	1.00
116_M	126_T	0.741	1.00
38_V	60_L	0.740	1.00
163_D	167_R	0.739	1.00
126_T	139_K	0.739	1.00
43_L	46_N	0.734	1.00
23_R	62_L	0.729	1.00
242_V	252_I	0.728	1.00
151_G	161_L	0.726	1.00
73_R	77_H	0.724	1.00
35_G	39_I	0.724	1.00
218_V	232_I	0.723	1.00
20_V	30_W	0.721	1.00
57_V	60_L	0.718	1.00
111_Y	137_L	0.718	1.00
222_N	227_E	0.716	1.00
159_F	202_I	0.713	1.00
213_S	221_W	0.711	1.00
34_D	56_L	0.707	1.00
166_L	170_F	0.702	1.00
115_T	119_H	0.701	1.00
184_K	188_M	0.700	1.00
104_K	152_W	0.700	1.00
147_S	164_T	0.697	1.00
67_R	80_N	0.696	1.00
149_G	199_G	0.695	1.00
79_L	84_G	0.694	1.00
132_L	135_Q	0.691	1.00
81_L	84_G	0.689	1.00
201_T	210_I	0.688	1.00
111_Y	114_G	0.687	1.00
199_G	241_S	0.687	1.00
153_D	159_F	0.684	1.00
29_F	43_L	0.681	1.00
149_G	200_M	0.678	1.00
234_L	256_T	0.676	1.00
103_G	112_W	0.676	1.00
100_F	103_G	0.675	1.00
202_I	223_P	0.673	1.00
171_Q	202_I	0.673	1.00
238_Y	257_M	0.672	1.00
96_G	118_L	0.665	1.00
28_L	40_V	0.664	1.00
95_N	98_N	0.664	1.00
40_V	62_L	0.658	1.00
30_W	68_I	0.656	1.00
207_Y	224_K	0.649	1.00
173_D	185_R	0.648	1.00
95_N	130_Y	0.647	1.00
215_G	219_S	0.647	1.00
34_D	37_K	0.638	1.00
75_G	91_E	0.638	1.00
207_Y	222_N	0.635	1.00
71_T	79_L	0.634	1.00
102_D	149_G	0.634	1.00
235_P	258_S	0.633	1.00
30_W	41_N	0.631	1.00
96_G	130_Y	0.630	1.00
131_K	173_D	0.626	1.00
59_S	103_G	0.624	1.00
39_I	50_Y	0.623	1.00
248_E	251_Q	0.623	1.00
239_V	254_I	0.622	1.00
105_C	132_L	0.622	1.00
155_D	158_L	0.618	1.00
171_Q	185_R	0.616	1.00
141_L	144_L	0.614	1.00
95_N	128_S	0.614	1.00
240_S	255_T	0.614	1.00
104_K	113_A	0.612	1.00
67_R	82_T	0.610	1.00
25_G	28_L	0.610	1.00
127_A	150_L	0.610	1.00
29_F	32_D	0.610	1.00
53_T	71_T	0.609	1.00
218_V	239_V	0.606	1.00
221_W	226_G	0.606	1.00
98_N	116_M	0.604	1.00
80_N	87_T	0.600	1.00
143_K	170_F	0.598	1.00
153_D	158_L	0.597	1.00
27_R	44_D	0.596	1.00
72_L	75_G	0.591	1.00
27_R	31_V	0.590	1.00
62_L	67_R	0.589	1.00
187_V	190_F	0.587	1.00
118_L	128_S	0.585	1.00
228_K	232_I	0.583	1.00
197_P	213_S	0.583	1.00
129_L	152_W	0.582	1.00
213_S	216_G	0.576	1.00
195_G	214_A	0.575	1.00
203_D	208_L	0.571	1.00
60_L	71_T	0.570	1.00
72_L	77_H	0.568	1.00
100_F	114_G	0.564	1.00
244_F	249_M	0.560	1.00
115_T	127_A	0.559	1.00
21_Y	43_L	0.559	1.00
23_R	26_N	0.556	1.00
112_W	130_Y	0.554	1.00
32_D	60_L	0.554	1.00
85_E	88_P	0.552	1.00
60_L	70_V	0.552	1.00
212_H	254_I	0.552	1.00
111_Y	115_T	0.548	0.99
69_I	105_C	0.547	0.99
27_R	40_V	0.547	0.99
239_V	256_T	0.544	0.99
67_R	70_V	0.543	0.99
170_F	184_K	0.538	0.99
63_I	109_D	0.537	0.99
21_Y	29_F	0.535	0.99
113_A	127_A	0.531	0.99
129_L	138_T	0.529	0.99
131_K	135_Q	0.528	0.99
174_F	184_K	0.527	0.99
168_K	191_K	0.526	0.99
239_V	242_V	0.526	0.99
254_I	258_S	0.525	0.99
164_T	172_F	0.522	0.99
77_H	89_Y	0.522	0.99
131_K	137_L	0.522	0.99
176_K	184_K	0.521	0.99
176_K	185_R	0.520	0.99
172_F	186_I	0.520	0.99
185_R	188_M	0.516	0.99
197_P	200_M	0.513	0.99
197_P	215_G	0.512	0.99
233_K	238_Y	0.509	0.99
30_W	69_I	0.507	0.99
202_I	244_F	0.506	0.99
63_I	78_T	0.505	0.99
60_L	66_K	0.503	0.99
26_N	43_L	0.503	0.99
64_D	109_D	0.502	0.99
208_L	212_H	0.501	0.99
19_P	31_V	0.501	0.99
193_E	219_S	0.500	0.99

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1vyhC	1	0.9333	100	0.213	Contact Map
3dm0A	1	0.9263	100	0.224	Contact Map
4j73A	1	0.9333	100	0.224	Contact Map
4u1eI	1	0.9333	100	0.238	Contact Map
5a1uD	1	0.9333	100	0.239	Contact Map
4wjuA	1	0.8526	100	0.24	Contact Map
4lg9A	1	0.9158	100	0.24	Contact Map
3sfzA	1	0.9544	100	0.24	Contact Map
3ow8A	3	0.8596	100	0.241	Contact Map
3zey7	1	0.9333	100	0.243	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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