Q97UH1 - SULSO - GREMLIN Contacts

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Q97UH1 - ABC transporter, permease

UniProt:	Q97UH1	Sequences: MRRDFLIKRGIYYIIVWFVGITIDFILPRLIPGSALASIIYSRIGGAGSNYGQNVQQEIQILVQQLGLAQYQQPLYIQYFNYLKEIVTLNFGVSLTEFPVSVSTIITEAAPWTFGIVIPAIVASFFIGNLLGRVAALSRGKISDYAILGLMMFLYTFPVFVTGEILIEVLAIDLHIFPTGGQYNTFQFLKPTMSLPFIWSVIYHAILPTLTLILFSLAGWIMGMRNNMIPILQEDYMNYYRLMGVSEKMVASKAYRLALLPNFTSFSISLGYSVLGAVAIEYLFNYAGLGYFFNQAITGLDYPLLSGIFFMLVTAMVLSNFVADIIYTIIDPRTSQEETEGV	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	342 (303)
Sequences:	17772 (11423)
Seq/√Len:	656.2

ECOLI - YEJB DDPB DPPB NIKB GSIC OPPB SAPB YEJE DPPC SAPC DDPC NIKC GSID OPPC
BACSU - APPB OPPB DPPB DPPC APPC OPPC
HALSA - Q9HMX0 Q9HMI2 Q9HMW3 Q9HMW2 Q9HMI1
SULSO - Q97UH1 Q97VF8 Q97UG4 Q97YQ0 Q97VK6 Q97YP1 Q97VK7 Q97UG3 Q97UH2 Q97VF6 Q97YP9 Q97YP2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
73_Q	77_I	3.520	1.00
73_Q	81_N	3.316	1.00
29_R	78_Q	3.242	1.00
80_F	84_K	2.937	1.00
84_K	88_T	2.863	1.00
56_Q	60_Q	2.637	1.00
64_Q	72_Q	2.627	1.00
228_M	254_A	2.617	1.00
21_I	79_Y	2.608	1.00
119_P	209_T	2.424	1.00
34_S	63_V	2.417	1.00
241_R	247_E	2.417	1.00
249_M	252_S	2.411	1.00
112_W	286_Y	2.375	1.00
237_M	254_A	2.266	1.00
22_T	79_Y	2.243	1.00
76_Y	80_F	2.144	1.00
291_Y	295_Q	2.141	1.00
105_I	292_F	2.071	1.00
101_S	104_T	2.037	1.00
267_S	323_A	2.000	1.00
231_I	236_Y	1.999	1.00
97_E	105_I	1.991	1.00
59_I	63_V	1.973	1.00
232_L	254_A	1.968	1.00
20_G	313_V	1.959	1.00
246_S	249_M	1.951	1.00
131_L	147_I	1.947	1.00
231_I	234_E	1.921	1.00
177_F	203_Y	1.918	1.00
58_E	61_I	1.889	1.00
123_A	213_I	1.878	1.00
177_F	206_I	1.874	1.00
171_A	179_T	1.827	1.00
126_F	130_L	1.812	1.00
248_K	251_A	1.803	1.00
263_F	330_I	1.801	1.00
138_S	141_K	1.795	1.00
77_I	81_N	1.773	1.00
61_I	65_Q	1.768	1.00
100_V	104_T	1.755	1.00
144_D	225_R	1.747	1.00
60_Q	64_Q	1.734	1.00
55_V	58_E	1.730	1.00
60_Q	63_V	1.704	1.00
81_N	84_K	1.702	1.00
142_I	146_A	1.687	1.00
102_V	307_G	1.642	1.00
239_Y	243_M	1.631	1.00
85_E	90_N	1.603	1.00
168_E	173_D	1.602	1.00
182_Q	286_Y	1.599	1.00
134_V	138_S	1.583	1.00
143_S	147_I	1.583	1.00
237_M	250_V	1.583	1.00
103_S	107_T	1.573	1.00
326_I	329_I	1.562	1.00
245_V	249_M	1.559	1.00
264_T	327_Y	1.548	1.00
293_F	308_I	1.518	1.00
13_Y	317_V	1.513	1.00
17_W	314_T	1.512	1.00
134_V	143_S	1.503	1.00
11_I	15_I	1.500	1.00
127_I	130_L	1.494	1.00
138_S	143_S	1.494	1.00
136_A	228_M	1.491	1.00
86_I	91_F	1.484	1.00
100_V	108_E	1.454	1.00
209_T	213_I	1.447	1.00
115_G	182_Q	1.440	1.00
124_S	216_S	1.436	1.00
169_V	173_D	1.426	1.00
231_I	258_A	1.421	1.00
166_L	211_T	1.420	1.00
62_L	66_L	1.419	1.00
231_I	237_M	1.402	1.00
161_V	165_I	1.401	1.00
112_W	182_Q	1.398	1.00
236_Y	257_L	1.396	1.00
127_I	217_L	1.394	1.00
243_M	253_K	1.383	1.00
18_F	79_Y	1.370	1.00
133_R	228_M	1.369	1.00
7_I	11_I	1.368	1.00
77_I	80_F	1.363	1.00
36_L	59_I	1.360	1.00
18_F	22_T	1.357	1.00
62_L	65_Q	1.352	1.00
235_D	238_N	1.333	1.00
81_N	85_E	1.324	1.00
296_A	304_L	1.322	1.00
105_I	304_L	1.317	1.00
74_P	77_I	1.299	1.00
169_V	174_L	1.290	1.00
292_F	304_L	1.287	1.00
128_G	224_M	1.281	1.00
125_F	266_F	1.280	1.00
34_S	37_A	1.265	1.00
140_G	144_D	1.260	1.00
213_I	217_L	1.259	1.00
21_I	82_Y	1.243	1.00
67_G	73_Q	1.241	1.00
132_G	136_A	1.238	1.00
93_V	101_S	1.238	1.00
91_F	307_G	1.234	1.00
121_I	266_F	1.223	1.00
170_L	174_L	1.218	1.00
157_F	162_T	1.218	1.00
260_L	327_Y	1.215	1.00
96_T	299_G	1.210	1.00
100_V	105_I	1.209	1.00
91_F	103_S	1.198	1.00
115_G	201_V	1.193	1.00
154_L	218_A	1.189	1.00
108_E	111_P	1.189	1.00
184_N	204_H	1.184	1.00
225_R	229_I	1.176	1.00
112_W	115_G	1.171	1.00
18_F	83_L	1.167	1.00
128_G	220_W	1.166	1.00
37_A	59_I	1.160	1.00
132_G	224_M	1.150	1.00
13_Y	321_F	1.137	1.00
22_T	26_I	1.136	1.00
136_A	229_I	1.134	1.00
224_M	262_N	1.132	1.00
308_I	312_L	1.129	1.00
294_N	298_T	1.123	1.00
165_I	169_V	1.123	1.00
67_G	81_N	1.123	1.00
73_Q	76_Y	1.111	1.00
325_I	329_I	1.108	1.00
6_L	325_I	1.106	1.00
115_G	119_P	1.105	1.00
94_S	301_D	1.101	1.00
166_L	207_L	1.100	1.00
231_I	254_A	1.100	1.00
17_W	317_V	1.097	1.00
147_I	221_I	1.096	1.00
319_S	323_A	1.093	1.00
135_A	147_I	1.090	1.00
137_L	247_E	1.087	1.00
136_A	232_L	1.083	1.00
10_G	321_F	1.081	1.00
115_G	205_A	1.079	1.00
104_T	108_E	1.079	1.00
223_G	265_S	1.074	1.00
211_T	215_F	1.074	1.00
61_I	64_Q	1.060	1.00
220_W	269_S	1.048	1.00
131_L	221_I	1.046	1.00
107_T	111_P	1.038	1.00
105_I	108_E	1.036	1.00
22_T	75_L	1.035	1.00
183_Y	204_H	1.032	1.00
79_Y	83_L	1.029	1.00
177_F	207_L	1.024	1.00
86_I	310_F	1.022	1.00
26_I	29_R	1.016	1.00
21_I	310_F	1.014	1.00
202_I	206_I	1.001	1.00
247_E	250_V	1.001	1.00
29_R	75_L	0.998	1.00
159_V	280_I	0.997	1.00
295_Q	299_G	0.996	1.00
19_V	23_I	0.992	1.00
322_V	326_I	0.986	1.00
271_G	316_M	0.985	1.00
144_D	229_I	0.982	1.00
245_V	250_V	0.973	1.00
57_Q	61_I	0.970	1.00
142_I	145_Y	0.969	1.00
205_A	209_T	0.966	1.00
40_I	62_L	0.963	1.00
120_A	213_I	0.963	1.00
139_R	233_Q	0.960	1.00
163_G	211_T	0.959	1.00
97_E	100_V	0.958	1.00
104_T	107_T	0.958	1.00
64_Q	73_Q	0.957	1.00
123_A	209_T	0.956	1.00
151_M	221_I	0.951	1.00
185_T	203_Y	0.951	1.00
117_V	273_S	0.951	1.00
206_I	210_L	0.950	1.00
224_M	228_M	0.947	1.00
33_G	300_L	0.944	1.00
26_I	75_L	0.943	1.00
36_L	40_I	0.923	1.00
130_L	133_R	0.923	1.00
282_Y	291_Y	0.920	1.00
10_G	14_I	0.920	1.00
93_V	99_P	0.918	1.00
118_I	122_V	0.917	1.00
57_Q	60_Q	0.917	1.00
263_F	323_A	0.916	1.00
270_L	319_S	0.915	1.00
140_G	145_Y	0.910	1.00
109_A	292_F	0.896	1.00
240_Y	250_V	0.895	1.00
241_R	250_V	0.894	1.00
137_L	251_A	0.894	1.00
85_E	88_T	0.892	1.00
84_K	87_V	0.891	1.00
318_L	322_V	0.888	1.00
15_I	18_F	0.886	1.00
163_G	283_L	0.883	1.00
106_I	307_G	0.882	1.00
29_R	302_Y	0.881	1.00
227_N	258_A	0.876	1.00
120_A	212_L	0.875	1.00
80_F	83_L	0.872	1.00
251_A	254_A	0.870	1.00
40_I	54_N	0.869	1.00
170_L	177_F	0.869	1.00
56_Q	59_I	0.865	1.00
292_F	296_A	0.864	1.00
162_T	214_L	0.864	1.00
146_A	150_L	0.863	1.00
95_L	303_P	0.863	1.00
128_G	132_G	0.859	1.00
228_M	258_A	0.855	1.00
82_Y	86_I	0.849	1.00
36_L	63_V	0.848	1.00
121_I	270_L	0.847	1.00
150_L	154_L	0.840	1.00
118_I	270_L	0.838	1.00
250_V	254_A	0.835	1.00
240_Y	245_V	0.835	1.00
83_L	86_I	0.830	1.00
328_T	334_T	0.829	1.00
258_A	261_P	0.827	1.00
277_A	280_I	0.819	1.00
210_L	213_I	0.818	1.00
106_I	308_I	0.815	1.00
132_G	228_M	0.814	1.00
120_A	124_S	0.813	1.00
24_D	309_F	0.813	1.00
128_G	221_I	0.806	1.00
130_L	134_V	0.804	1.00
119_P	286_Y	0.802	1.00
139_R	229_I	0.802	1.00
125_F	130_L	0.802	1.00
114_F	118_I	0.800	1.00
36_L	62_L	0.799	1.00
138_S	247_E	0.794	1.00
113_T	117_V	0.794	1.00
327_Y	334_T	0.792	1.00
302_Y	306_S	0.790	1.00
129_N	224_M	0.789	1.00
145_Y	148_L	0.788	1.00
325_I	328_T	0.787	1.00
127_I	131_L	0.786	1.00
182_Q	205_A	0.784	1.00
148_L	221_I	0.783	1.00
314_T	318_L	0.781	1.00
143_S	146_A	0.780	1.00
159_V	214_L	0.772	1.00
182_Q	201_V	0.772	1.00
84_K	89_L	0.771	1.00
5_F	328_T	0.764	1.00
131_L	217_L	0.763	1.00
170_L	176_I	0.759	1.00
217_L	221_I	0.759	1.00
5_F	336_Q	0.759	1.00
170_L	207_L	0.757	1.00
115_G	118_I	0.756	1.00
168_E	172_I	0.755	1.00
111_P	114_F	0.754	1.00
211_T	280_I	0.753	1.00
116_I	209_T	0.750	1.00
22_T	76_Y	0.748	1.00
11_I	14_I	0.740	1.00
154_L	214_L	0.737	1.00
133_R	255_Y	0.734	1.00
224_M	227_N	0.734	1.00
87_V	90_N	0.732	1.00
16_V	317_V	0.729	1.00
306_S	309_F	0.728	1.00
240_Y	253_K	0.728	1.00
206_I	209_T	0.727	1.00
9_R	324_D	0.724	1.00
167_I	179_T	0.721	1.00
58_E	62_L	0.719	1.00
155_Y	218_A	0.718	1.00
36_L	66_L	0.716	1.00
180_G	285_N	0.714	1.00
224_M	258_A	0.714	1.00
102_V	292_F	0.710	1.00
282_Y	294_N	0.707	1.00
83_L	87_V	0.706	1.00
60_Q	72_Q	0.706	1.00
307_G	311_M	0.706	1.00
26_I	30_L	0.706	1.00
173_D	176_I	0.705	1.00
295_Q	298_T	0.701	1.00
54_N	59_I	0.700	1.00
110_A	114_F	0.699	1.00
39_I	62_L	0.695	1.00
124_S	213_I	0.694	1.00
181_G	204_H	0.692	1.00
263_F	326_I	0.691	1.00
129_N	262_N	0.689	1.00
127_I	213_I	0.689	1.00
201_V	204_H	0.689	1.00
292_F	308_I	0.684	1.00
287_A	291_Y	0.683	1.00
216_S	219_G	0.682	1.00
328_T	335_S	0.681	1.00
316_M	320_N	0.681	1.00
240_Y	243_M	0.681	1.00
203_Y	206_I	0.680	1.00
14_I	18_F	0.680	1.00
109_A	291_Y	0.679	1.00
293_F	312_L	0.679	1.00
25_F	29_R	0.677	1.00
293_F	296_A	0.675	1.00
151_M	218_A	0.675	1.00
183_Y	201_V	0.673	1.00
113_T	277_A	0.670	1.00
119_P	123_A	0.665	1.00
184_N	201_V	0.665	1.00
119_P	206_I	0.664	1.00
178_P	181_G	0.662	1.00
227_N	231_I	0.660	1.00
42_S	54_N	0.659	1.00
282_Y	295_Q	0.659	1.00
147_I	151_M	0.659	1.00
63_V	72_Q	0.657	1.00
63_V	68_L	0.656	1.00
37_A	41_Y	0.656	1.00
146_A	149_G	0.654	1.00
212_L	277_A	0.651	1.00
224_M	255_Y	0.650	1.00
135_A	144_D	0.650	1.00
107_T	110_A	0.648	1.00
111_P	115_G	0.645	1.00
77_I	84_K	0.644	1.00
105_I	109_A	0.644	1.00
119_P	205_A	0.640	1.00
178_P	204_H	0.639	1.00
236_Y	240_Y	0.638	1.00
166_L	210_L	0.637	1.00
24_D	28_P	0.633	1.00
28_P	305_L	0.628	1.00
28_P	31_I	0.627	1.00
106_I	289_L	0.626	1.00
174_L	177_F	0.625	1.00
75_L	78_Q	0.623	1.00
94_S	100_V	0.620	1.00
138_S	144_D	0.620	1.00
246_S	250_V	0.619	1.00
182_Q	185_T	0.617	1.00
220_W	262_N	0.616	1.00
323_A	327_Y	0.614	1.00
267_S	319_S	0.612	1.00
144_D	148_L	0.612	1.00
54_N	58_E	0.612	1.00
159_V	215_F	0.611	1.00
29_R	306_S	0.607	1.00
278_V	294_N	0.606	1.00
13_Y	16_V	0.605	1.00
123_A	210_L	0.603	1.00
122_V	126_F	0.601	1.00
312_L	316_M	0.601	1.00
102_V	304_L	0.601	1.00
65_Q	99_P	0.600	1.00
97_E	304_L	0.600	1.00
230_P	234_E	0.600	1.00
133_R	251_A	0.599	1.00
315_A	319_S	0.597	1.00
149_G	153_F	0.595	1.00
296_A	305_L	0.593	1.00
141_K	144_D	0.593	1.00
125_F	220_W	0.593	1.00
260_L	334_T	0.592	1.00
305_L	309_F	0.591	1.00
245_V	253_K	0.589	1.00
21_I	83_L	0.589	1.00
271_G	315_A	0.588	1.00
141_K	146_A	0.586	1.00
121_I	125_F	0.585	1.00
36_L	39_I	0.585	1.00
269_S	272_Y	0.584	1.00
133_R	137_L	0.583	1.00
157_F	161_V	0.583	1.00
218_A	221_I	0.581	1.00
278_V	282_Y	0.580	1.00
219_G	265_S	0.579	1.00
117_V	121_I	0.578	1.00
93_V	97_E	0.572	1.00
141_K	145_Y	0.570	1.00
251_A	256_R	0.569	1.00
159_V	162_T	0.569	1.00
136_A	144_D	0.567	1.00
210_L	214_L	0.566	1.00
8_K	11_I	0.565	1.00
271_G	319_S	0.563	1.00
259_L	263_F	0.562	1.00
117_V	277_A	0.560	1.00
36_L	54_N	0.559	1.00
115_G	286_Y	0.557	1.00
220_W	224_M	0.556	1.00
259_L	262_N	0.555	1.00
24_D	29_R	0.555	1.00
304_L	308_I	0.555	1.00
161_V	164_E	0.554	1.00
219_G	223_G	0.554	1.00
207_L	211_T	0.551	1.00
237_M	241_R	0.548	1.00
135_A	148_L	0.547	1.00
251_A	255_Y	0.546	1.00
35_A	38_S	0.546	1.00
220_W	265_S	0.543	1.00
321_F	325_I	0.543	1.00
294_N	297_I	0.542	1.00
171_A	175_H	0.542	1.00
313_V	317_V	0.542	1.00
238_N	242_L	0.541	1.00
213_I	216_S	0.541	1.00
75_L	79_Y	0.539	1.00
219_G	269_S	0.539	1.00
126_F	129_N	0.539	1.00
327_Y	331_D	0.538	1.00
125_F	129_N	0.532	1.00
124_S	220_W	0.529	0.99
328_T	336_Q	0.528	0.99
303_P	306_S	0.527	0.99
184_N	203_Y	0.526	0.99
317_V	320_N	0.525	0.99
117_V	270_L	0.523	0.99
227_N	230_P	0.520	0.99
332_P	335_S	0.520	0.99
170_L	173_D	0.519	0.99
15_I	19_V	0.519	0.99
235_D	239_Y	0.519	0.99
159_V	163_G	0.519	0.99
20_G	24_D	0.516	0.99
114_F	274_V	0.515	0.99
12_Y	16_V	0.514	0.99
137_L	232_L	0.513	0.99
223_G	227_N	0.513	0.99
297_I	305_L	0.513	0.99
271_G	323_A	0.511	0.99
263_F	327_Y	0.511	0.99
55_V	59_I	0.506	0.99
25_F	75_L	0.506	0.99
35_A	302_Y	0.505	0.99
18_F	21_I	0.504	0.99
220_W	266_F	0.504	0.99
135_A	225_R	0.502	0.99
215_F	218_A	0.502	0.99
177_F	180_G	0.500	0.99

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3tuiA	2	0.6082	99.9	0.702	Contact Map
3rlfF	1	0.8012	99.8	0.725	Contact Map
4tquM	1	0.7895	99.8	0.727	Contact Map
4ymuD	2	0.6111	99.8	0.731	Contact Map
3d31C	2	0.7193	99.8	0.734	Contact Map
2onkC	2	0.7047	99.8	0.734	Contact Map
4tquN	1	0.7573	99.7	0.753	Contact Map
3rlfG	1	0.8129	99.7	0.766	Contact Map
4lz6A	1	0.2544	18.7	0.96	Contact Map
2yevC	1	0.0994	17	0.961	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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