Q97UG8 - SULSO - GREMLIN Contacts

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Q97UG8 - Uncharacterized protein

UniProt:	Q97UG8	Sequences: MRLSNGDVIVFAGDSITDREKSNNAPLGYGYVNLFYNLMLAKHPETKITVINSGISGNTVIDLIDRWDDDVLSYKPNWISILIGINDLHKFLGGLTTFNPENYYENLRRLLKYTRKVLNNVNFILMTPFYISRNKLEGGFRAKVLELLPQYIEKVRLLSNEFSATYIDLHDTFKKLVNIREPNVYAPEPVHPNFTGHMVIALKLLEALEI	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	210 (178)
Sequences:	10540 (7771)
Seq/√Len:	582.4

ECOLI - TESA
BACSU - YQEF RHGT1 YPMR RHGT2 LIPC YXIM
SULSO - Q97UG8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
127_T	166_Y	3.514	1.00
108_R	162_F	3.287	1.00
112_K	162_F	3.192	1.00
104_Y	157_L	3.114	1.00
63_L	83_I	2.838	1.00
108_R	161_E	2.716	1.00
60_V	106_N	2.703	1.00
79_I	114_T	2.621	1.00
11_F	17_T	2.519	1.00
58_N	66_R	2.387	1.00
68_D	113_Y	2.212	1.00
159_S	166_Y	2.136	1.00
79_I	121_V	2.080	1.00
9_I	80_S	2.055	1.00
108_R	112_K	2.002	1.00
104_Y	154_K	1.904	1.00
64_I	109_R	1.859	1.00
8_V	49_T	1.778	1.00
18_D	56_S	1.770	1.00
128_P	152_I	1.733	1.00
122_N	165_T	1.725	1.00
168_D	171_D	1.709	1.00
199_V	202_L	1.704	1.00
115_R	162_F	1.684	1.00
38_L	205_L	1.631	1.00
34_L	198_M	1.625	1.00
198_M	202_L	1.617	1.00
12_A	63_L	1.608	1.00
192_P	200_I	1.601	1.00
111_L	125_L	1.600	1.00
11_F	80_S	1.590	1.00
125_L	159_S	1.550	1.00
58_N	62_D	1.547	1.00
154_K	157_L	1.546	1.00
16_I	192_P	1.542	1.00
203_K	206_E	1.534	1.00
126_M	204_L	1.533	1.00
99_N	102_N	1.513	1.00
127_T	168_D	1.480	1.00
156_R	166_Y	1.476	1.00
127_T	156_R	1.447	1.00
23_N	194_F	1.424	1.00
169_L	200_I	1.419	1.00
29_Y	194_F	1.409	1.00
158_L	162_F	1.402	1.00
111_L	164_A	1.401	1.00
17_T	54_G	1.370	1.00
64_I	106_N	1.365	1.00
103_Y	151_Y	1.360	1.00
20_E	56_S	1.349	1.00
195_T	198_M	1.345	1.00
113_Y	117_V	1.291	1.00
38_L	202_L	1.281	1.00
60_V	103_Y	1.280	1.00
172_T	203_K	1.276	1.00
104_Y	108_R	1.273	1.00
109_R	113_Y	1.267	1.00
167_I	207_A	1.266	1.00
60_V	107_L	1.260	1.00
153_E	157_L	1.252	1.00
111_L	158_L	1.231	1.00
114_T	121_V	1.227	1.00
78_W	124_I	1.226	1.00
11_F	32_V	1.213	1.00
16_I	85_I	1.206	1.00
22_S	194_F	1.203	1.00
60_V	110_L	1.176	1.00
156_R	160_N	1.174	1.00
64_I	113_Y	1.172	1.00
125_L	155_V	1.167	1.00
61_I	102_N	1.167	1.00
7_D	48_I	1.164	1.00
80_S	124_I	1.157	1.00
10_V	76_P	1.144	1.00
108_R	158_L	1.130	1.00
111_L	162_F	1.124	1.00
7_D	77_N	1.119	1.00
107_L	155_V	1.094	1.00
104_Y	161_E	1.068	1.00
66_R	70_D	1.062	1.00
67_W	114_T	1.055	1.00
98_F	102_N	1.034	1.00
100_P	150_Q	1.033	1.00
8_V	51_I	1.032	1.00
102_N	105_E	1.031	1.00
17_T	82_L	1.027	1.00
59_T	62_D	1.026	1.00
81_I	110_L	1.023	1.00
148_L	152_I	1.021	1.00
112_K	116_K	1.021	1.00
124_I	167_I	1.018	1.00
85_I	190_V	1.018	1.00
129_F	192_P	1.017	1.00
8_V	77_N	1.014	1.00
80_S	126_M	1.014	1.00
89_H	190_V	1.009	1.00
107_L	111_L	0.975	1.00
105_E	108_R	0.974	1.00
34_L	38_L	0.971	1.00
199_V	203_K	0.965	1.00
117_V	121_V	0.965	1.00
60_V	83_I	0.961	1.00
103_Y	155_V	0.958	1.00
72_L	117_V	0.958	1.00
152_I	155_V	0.953	1.00
103_Y	154_K	0.949	1.00
105_E	109_R	0.944	1.00
170_H	174_K	0.942	1.00
34_L	37_N	0.940	1.00
82_L	129_F	0.939	1.00
201_A	205_L	0.935	1.00
78_W	122_N	0.920	1.00
10_V	53_S	0.918	1.00
147_L	150_Q	0.912	1.00
70_D	73_S	0.910	1.00
61_I	106_N	0.906	1.00
76_P	121_V	0.902	1.00
197_H	200_I	0.901	1.00
31_Y	197_H	0.891	1.00
111_L	123_F	0.882	1.00
171_D	174_K	0.881	1.00
157_L	161_E	0.877	1.00
113_Y	116_K	0.870	1.00
30_G	33_N	0.865	1.00
67_W	110_L	0.861	1.00
82_L	126_M	0.857	1.00
106_N	110_L	0.844	1.00
102_N	106_N	0.843	1.00
36_Y	40_L	0.843	1.00
85_I	131_I	0.839	1.00
38_L	201_A	0.834	1.00
61_I	65_D	0.831	1.00
69_D	72_L	0.819	1.00
66_R	69_D	0.818	1.00
125_L	158_L	0.814	1.00
149_P	153_E	0.812	1.00
172_T	175_K	0.810	1.00
87_D	106_N	0.808	1.00
60_V	64_I	0.802	1.00
23_N	27_L	0.798	1.00
67_W	70_D	0.789	1.00
203_K	207_A	0.789	1.00
88_L	131_I	0.788	1.00
146_E	150_Q	0.786	1.00
38_L	198_M	0.781	1.00
34_L	201_A	0.778	1.00
158_L	161_E	0.778	1.00
114_T	123_F	0.777	1.00
100_P	104_Y	0.774	1.00
53_S	74_Y	0.772	1.00
171_D	175_K	0.771	1.00
150_Q	154_K	0.770	1.00
33_N	36_Y	0.770	1.00
153_E	156_R	0.767	1.00
31_Y	204_L	0.766	1.00
100_P	154_K	0.765	1.00
40_L	47_K	0.763	1.00
150_Q	153_E	0.763	1.00
126_M	169_L	0.761	1.00
11_F	35_F	0.761	1.00
17_T	80_S	0.758	1.00
101_E	154_K	0.752	1.00
101_E	105_E	0.749	1.00
104_Y	158_L	0.743	1.00
159_S	164_A	0.743	1.00
35_F	205_L	0.740	1.00
63_L	110_L	0.739	1.00
107_L	125_L	0.738	1.00
152_I	156_R	0.738	1.00
68_D	72_L	0.738	1.00
35_F	201_A	0.736	1.00
109_R	112_K	0.720	1.00
156_R	168_D	0.716	1.00
111_L	115_R	0.715	1.00
23_N	29_Y	0.714	1.00
85_I	128_P	0.713	1.00
39_M	48_I	0.710	1.00
14_D	84_G	0.705	1.00
80_S	204_L	0.704	1.00
82_L	85_I	0.703	1.00
145_L	149_P	0.702	1.00
22_S	30_G	0.694	1.00
55_I	66_R	0.693	1.00
48_I	78_W	0.686	1.00
32_V	36_Y	0.678	1.00
55_I	58_N	0.678	1.00
126_M	200_I	0.677	1.00
123_F	164_A	0.676	1.00
68_D	73_S	0.672	1.00
195_T	199_V	0.670	1.00
167_I	204_L	0.668	1.00
62_D	65_D	0.666	1.00
9_I	78_W	0.662	1.00
103_Y	107_L	0.661	1.00
145_L	148_L	0.659	1.00
158_L	164_A	0.655	1.00
112_K	115_R	0.654	1.00
129_F	173_F	0.649	1.00
98_F	103_Y	0.646	1.00
135_K	138_G	0.634	1.00
8_V	74_Y	0.632	1.00
107_L	158_L	0.629	1.00
130_Y	173_F	0.629	1.00
101_E	104_Y	0.627	1.00
34_L	197_H	0.624	1.00
33_N	37_N	0.622	1.00
127_T	155_V	0.622	1.00
48_I	77_N	0.614	1.00
29_Y	37_N	0.610	1.00
68_D	71_V	0.608	1.00
151_Y	155_V	0.608	1.00
106_N	109_R	0.607	1.00
9_I	50_V	0.606	1.00
14_D	82_L	0.605	1.00
88_L	147_L	0.598	1.00
162_F	165_T	0.598	1.00
36_Y	50_V	0.597	1.00
29_Y	34_L	0.595	1.00
185_Y	199_V	0.593	1.00
15_S	191_H	0.593	1.00
64_I	110_L	0.592	1.00
53_S	70_D	0.591	1.00
120_N	123_F	0.590	1.00
35_F	38_L	0.585	1.00
172_T	199_V	0.577	1.00
192_P	197_H	0.574	1.00
124_I	165_T	0.573	1.00
11_F	50_V	0.573	1.00
86_N	191_H	0.573	1.00
22_S	197_H	0.567	1.00
16_I	129_F	0.567	1.00
64_I	68_D	0.566	1.00
185_Y	189_P	0.566	1.00
53_S	71_V	0.565	0.99
51_I	74_Y	0.562	0.99
124_I	207_A	0.558	0.99
155_V	166_Y	0.557	0.99
110_L	113_Y	0.556	0.99
148_L	151_Y	0.555	0.99
64_I	105_E	0.555	0.99
155_V	158_L	0.554	0.99
6_G	49_T	0.552	0.99
144_V	147_L	0.548	0.99
85_I	89_H	0.546	0.99
167_I	203_K	0.543	0.99
115_R	123_F	0.542	0.99
183_N	195_T	0.540	0.99
16_I	82_L	0.538	0.99
12_A	83_I	0.538	0.99
6_G	47_K	0.533	0.99
79_I	123_F	0.531	0.99
88_L	148_L	0.527	0.99
152_I	170_H	0.526	0.99
131_I	144_V	0.524	0.99
19_R	30_G	0.522	0.99
7_D	78_W	0.518	0.99
19_R	197_H	0.516	0.99
129_F	152_I	0.514	0.99
101_E	150_Q	0.511	0.99
159_S	162_F	0.511	0.99
88_L	103_Y	0.510	0.99
28_G	33_N	0.510	0.99
12_A	53_S	0.510	0.99
11_F	54_G	0.506	0.99
194_F	198_M	0.504	0.99
10_V	74_Y	0.501	0.99
157_L	160_N	0.500	0.99

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4q9aA	2	0.9905	100	0.208	Contact Map
4jhlA	5	0.9667	100	0.229	Contact Map
3rjtA	2	0.9667	100	0.242	Contact Map
4rw0A	1	0.8571	100	0.281	Contact Map
3dc7A	3	0.9333	100	0.286	Contact Map
2o14A	1	0.9381	100	0.286	Contact Map
1k7cA	1	0.8952	100	0.288	Contact Map
2q0qA	5	0.8905	100	0.291	Contact Map
4q7qA	1	0.9524	100	0.292	Contact Map
3bzwA	4	0.9571	100	0.293	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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