Q97UB0 - SULSO - GREMLIN Contacts

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Q97UB0 - Sec-independent protein translocase protein TatC

UniProt:	Q97UB0	Sequences: MEEKAKERGKEEKSFFEEKPLLDHLRELAIRLRRIIAALAISFFFYFMTGYEWINIDPLQIPFLGISISKIPIPYPSFYDSIAVQVTKFFIYHELPANTKIIIINLFDPIYASLYVSLYLALLTSLPLIIRETWAFLAPGLYKHEKKIFKLTMIPAFLLFFSGSLFAFLILIPLMFDMISFYTESLGPAVEPTISLSSFISTTFLLMGSLGLAFEMPLIMIGLTHLRIVKASTWRNYWRWGVLVSFIIAWIISPGTTGGVMETIIGLSLSSLYFLGMGISFLVERKRES	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	289 (237)
Sequences:	3347 (2132)
Seq/√Len:	138.5

ECOLI - TATC
BACSU - TATCY TATCD
HALSA - Q9HN36
SULSO - Q97UB0 Q97ZU3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
224_T	280_S	4.246	1.00
238_W	274_F	4.053	1.00
38_A	128_L	4.005	1.00
30_I	34_R	3.729	1.00
235_R	281_F	3.709	1.00
34_R	128_L	3.362	1.00
131_R	135_A	3.112	1.00
246_F	266_G	2.970	1.00
231_A	284_E	2.776	1.00
43_F	121_A	2.638	1.00
27_E	31_R	2.619	1.00
241_G	273_Y	2.266	1.00
35_I	129_I	2.226	1.00
100_K	191_E	2.159	1.00
46_Y	120_L	2.092	1.00
230_K	233_T	2.088	1.00
44_F	48_M	2.034	1.00
220_M	234_W	2.023	1.00
231_A	280_S	1.996	1.00
223_L	228_I	1.986	1.00
255_G	261_M	1.960	1.00
165_L	169_L	1.954	1.00
33_R	37_A	1.954	1.00
183_T	192_P	1.913	1.00
141_L	145_E	1.911	1.00
34_R	132_E	1.889	1.00
104_I	195_S	1.882	1.00
49_T	52_E	1.858	1.00
273_Y	277_M	1.856	1.00
102_I	193_T	1.852	1.00
242_V	270_S	1.834	1.00
53_W	120_L	1.831	1.00
240_W	244_V	1.801	1.00
42_S	121_A	1.796	1.00
108_D	195_S	1.795	1.00
32_L	36_I	1.787	1.00
153_M	157_F	1.784	1.00
121_A	125_S	1.777	1.00
39_L	118_L	1.763	1.00
245_S	269_L	1.757	1.00
20_P	23_D	1.752	1.00
160_F	211_G	1.717	1.00
31_R	135_A	1.700	1.00
246_F	262_E	1.695	1.00
229_V	234_W	1.692	1.00
257_T	262_E	1.672	1.00
235_R	238_W	1.651	1.00
105_N	108_D	1.643	1.00
156_A	215_E	1.621	1.00
31_R	132_E	1.612	1.00
237_Y	240_W	1.611	1.00
125_S	129_I	1.562	1.00
248_I	252_I	1.561	1.00
42_S	124_T	1.544	1.00
231_A	281_F	1.532	1.00
106_L	176_F	1.525	1.00
232_S	236_N	1.524	1.00
233_T	237_Y	1.522	1.00
224_T	231_A	1.506	1.00
161_F	165_L	1.503	1.00
138_A	146_K	1.501	1.00
35_I	125_S	1.498	1.00
231_A	235_R	1.495	1.00
103_I	109_P	1.446	1.00
235_R	274_F	1.445	1.00
45_F	48_M	1.412	1.00
19_K	23_D	1.406	1.00
34_R	37_A	1.399	1.00
38_A	125_S	1.389	1.00
106_L	109_P	1.373	1.00
42_S	125_S	1.358	1.00
158_L	162_S	1.333	1.00
195_S	198_S	1.319	1.00
225_H	283_V	1.314	1.00
113_S	182_Y	1.297	1.00
221_I	283_V	1.289	1.00
250_W	262_E	1.281	1.00
39_L	125_S	1.281	1.00
126_L	130_I	1.274	1.00
243_L	247_I	1.274	1.00
39_L	129_I	1.246	1.00
278_G	282_L	1.240	1.00
232_S	235_R	1.212	1.00
255_G	262_E	1.209	1.00
105_N	198_S	1.208	1.00
106_L	202_T	1.205	1.00
252_I	265_I	1.199	1.00
180_S	192_P	1.199	1.00
234_W	273_Y	1.193	1.00
220_M	280_S	1.182	1.00
220_M	276_G	1.176	1.00
164_S	207_M	1.148	0.99
26_R	30_I	1.143	0.99
41_I	45_F	1.142	0.99
136_F	139_P	1.142	0.99
274_F	278_G	1.138	0.99
104_I	193_T	1.134	0.99
263_T	267_L	1.127	0.99
172_I	203_T	1.125	0.99
50_G	113_S	1.104	0.99
24_H	145_E	1.104	0.99
122_L	129_I	1.096	0.99
45_F	49_T	1.091	0.99
107_F	179_I	1.084	0.99
245_S	270_S	1.081	0.99
56_I	169_L	1.080	0.99
35_I	38_A	1.080	0.99
140_G	145_E	1.072	0.99
164_S	211_G	1.070	0.99
143_K	147_K	1.065	0.99
34_R	131_R	1.055	0.99
231_A	277_M	1.040	0.99
36_I	40_A	1.034	0.99
237_Y	241_G	1.030	0.99
37_A	41_I	1.024	0.99
216_M	245_S	1.015	0.99
103_I	196_L	0.999	0.98
103_I	190_V	0.998	0.98
47_F	113_S	0.998	0.98
24_H	136_F	0.992	0.98
37_A	40_A	0.985	0.98
27_E	132_E	0.984	0.98
249_A	253_S	0.981	0.98
132_E	135_A	0.977	0.98
235_R	277_M	0.966	0.98
47_F	114_L	0.965	0.98
239_R	262_E	0.963	0.98
31_R	140_G	0.953	0.98
250_W	253_S	0.940	0.98
174_L	177_D	0.929	0.97
24_H	27_E	0.928	0.97
46_Y	124_T	0.920	0.97
122_L	126_L	0.917	0.97
232_S	281_F	0.917	0.97
253_S	265_I	0.917	0.97
172_I	176_F	0.913	0.97
136_F	140_G	0.912	0.97
40_A	44_F	0.905	0.97
216_M	276_G	0.896	0.97
190_V	196_L	0.893	0.97
175_M	261_M	0.890	0.97
103_I	108_D	0.889	0.97
249_A	252_I	0.885	0.96
229_V	233_T	0.881	0.96
39_L	122_L	0.881	0.96
24_H	28_L	0.880	0.96
176_F	202_T	0.874	0.96
106_L	194_I	0.874	0.96
163_G	211_G	0.873	0.96
24_H	139_P	0.872	0.96
238_W	278_G	0.865	0.96
136_F	145_E	0.861	0.96
209_S	252_I	0.860	0.96
242_V	274_F	0.859	0.96
175_M	179_I	0.858	0.96
238_W	242_V	0.858	0.96
242_V	266_G	0.857	0.96
245_S	249_A	0.857	0.96
234_W	277_M	0.856	0.96
253_S	262_E	0.854	0.96
262_E	266_G	0.854	0.96
280_S	284_E	0.852	0.96
123_L	164_S	0.846	0.95
83_A	87_T	0.845	0.95
134_W	149_F	0.841	0.95
105_N	197_S	0.839	0.95
244_V	247_I	0.838	0.95
278_G	281_F	0.834	0.95
126_L	129_I	0.831	0.95
42_S	46_Y	0.827	0.95
226_L	229_V	0.819	0.95
213_A	269_L	0.819	0.95
103_I	192_P	0.810	0.94
179_I	216_M	0.808	0.94
216_M	269_L	0.804	0.94
27_E	136_F	0.799	0.94
103_I	183_T	0.794	0.94
156_A	218_L	0.793	0.94
84_V	93_H	0.783	0.93
48_M	52_E	0.782	0.93
183_T	190_V	0.777	0.93
256_T	261_M	0.775	0.93
47_F	51_Y	0.774	0.93
48_M	117_S	0.774	0.93
150_K	154_I	0.773	0.93
256_T	262_E	0.770	0.93
110_I	182_Y	0.769	0.93
174_L	264_I	0.767	0.92
24_H	140_G	0.761	0.92
83_A	86_V	0.761	0.92
115_Y	201_S	0.761	0.92
249_A	266_G	0.752	0.92
43_F	118_L	0.750	0.92
109_P	182_Y	0.748	0.91
84_V	87_T	0.746	0.91
148_I	151_L	0.740	0.91
32_L	35_I	0.737	0.91
29_A	33_R	0.737	0.91
51_Y	55_N	0.735	0.91
157_F	161_F	0.731	0.91
220_M	229_V	0.729	0.90
180_S	194_I	0.725	0.90
21_L	30_I	0.723	0.90
149_F	153_M	0.721	0.90
239_R	242_V	0.706	0.89
142_Y	145_E	0.705	0.89
149_F	157_F	0.696	0.88
51_Y	113_S	0.694	0.88
46_Y	49_T	0.691	0.88
126_L	157_F	0.689	0.88
246_F	270_S	0.686	0.88
38_A	129_I	0.682	0.87
111_Y	115_Y	0.679	0.87
148_I	222_G	0.679	0.87
177_D	181_F	0.677	0.87
46_Y	121_A	0.676	0.87
117_S	120_L	0.676	0.87
201_S	205_L	0.671	0.86
47_F	117_S	0.671	0.86
159_L	272_L	0.671	0.86
166_F	276_G	0.669	0.86
109_P	192_P	0.668	0.86
52_E	56_I	0.666	0.86
138_A	142_Y	0.664	0.86
51_Y	103_I	0.661	0.86
108_D	112_A	0.659	0.85
15_F	18_E	0.658	0.85
122_L	208_G	0.656	0.85
104_I	108_D	0.656	0.85
176_F	182_Y	0.655	0.85
122_L	156_A	0.654	0.85
42_S	45_F	0.653	0.85
34_R	38_A	0.652	0.85
205_L	208_G	0.649	0.85
136_F	141_L	0.646	0.84
196_L	218_L	0.641	0.84
206_L	210_L	0.640	0.84
112_A	116_V	0.635	0.83
130_I	156_A	0.633	0.83
257_T	261_M	0.633	0.83
221_I	279_I	0.632	0.83
213_A	265_I	0.631	0.83
128_L	132_E	0.628	0.83
139_P	199_F	0.626	0.82
54_I	116_V	0.626	0.82
31_R	139_P	0.623	0.82
101_I	167_A	0.622	0.82
139_P	145_E	0.620	0.82
214_F	272_L	0.620	0.82
270_S	274_F	0.619	0.82
224_T	234_W	0.615	0.81
22_L	25_L	0.613	0.81
242_V	246_F	0.612	0.81
35_I	121_A	0.609	0.81
121_A	148_I	0.608	0.81
45_F	124_T	0.608	0.81
267_L	271_S	0.607	0.80
225_H	279_I	0.605	0.80
109_P	190_V	0.604	0.80
100_K	193_T	0.598	0.79
167_A	210_L	0.594	0.79
174_L	262_E	0.593	0.79
137_L	149_F	0.592	0.79
155_P	218_L	0.590	0.79
205_L	261_M	0.590	0.79
160_F	163_G	0.589	0.78
224_T	284_E	0.587	0.78
118_L	122_L	0.587	0.78
225_H	286_K	0.582	0.78
119_Y	204_F	0.582	0.78
175_M	239_R	0.580	0.77
160_F	215_E	0.579	0.77
24_H	31_R	0.577	0.77
167_A	206_L	0.576	0.77
113_S	184_E	0.576	0.77
180_S	183_T	0.576	0.77
167_A	172_I	0.572	0.76
174_L	276_G	0.569	0.76
24_H	141_L	0.568	0.76
27_E	139_P	0.568	0.76
21_L	26_R	0.564	0.75
116_V	161_F	0.564	0.75
162_S	165_L	0.562	0.75
52_E	55_N	0.562	0.75
93_H	96_P	0.562	0.75
255_G	263_T	0.560	0.75
101_I	117_S	0.558	0.75
16_F	19_K	0.555	0.74
108_D	197_S	0.553	0.74
119_Y	123_L	0.552	0.74
200_I	278_G	0.552	0.74
109_P	134_W	0.551	0.74
49_T	55_N	0.551	0.74
192_P	195_S	0.550	0.74
274_F	277_M	0.549	0.73
54_I	113_S	0.548	0.73
39_L	42_S	0.548	0.73
264_I	269_L	0.547	0.73
199_F	203_T	0.545	0.73
246_F	263_T	0.545	0.73
153_M	200_I	0.545	0.73
112_A	196_L	0.544	0.73
210_L	265_I	0.542	0.72
164_S	212_L	0.541	0.72
130_I	134_W	0.539	0.72
132_E	145_E	0.539	0.72
108_D	194_I	0.539	0.72
238_W	277_M	0.539	0.72
231_A	238_W	0.538	0.72
223_L	229_V	0.538	0.72
49_T	53_W	0.537	0.72
253_S	261_M	0.537	0.72
57_D	220_M	0.535	0.72
261_M	265_I	0.535	0.72
151_L	154_I	0.534	0.71
21_L	136_F	0.533	0.71
171_L	235_R	0.533	0.71
56_I	161_F	0.532	0.71
107_F	111_Y	0.531	0.71
43_F	117_S	0.530	0.71
123_L	208_G	0.528	0.71
19_K	22_L	0.528	0.71
27_E	135_A	0.527	0.70
27_E	140_G	0.527	0.70
220_M	272_L	0.525	0.70
119_Y	122_L	0.525	0.70
163_G	214_F	0.524	0.70
134_W	138_A	0.524	0.70
127_P	163_G	0.523	0.70
277_M	280_S	0.522	0.70
229_V	276_G	0.522	0.70
251_I	257_T	0.522	0.70
106_L	179_I	0.521	0.70
54_I	200_I	0.520	0.69
277_M	284_E	0.518	0.69
242_V	245_S	0.518	0.69
19_K	27_E	0.518	0.69
146_K	150_K	0.517	0.69
271_S	282_L	0.517	0.69
57_D	203_T	0.516	0.69
176_F	193_T	0.516	0.69
167_A	211_G	0.515	0.69
236_N	243_L	0.514	0.68
95_L	219_I	0.512	0.68
249_A	260_V	0.512	0.68
237_Y	244_V	0.511	0.68
132_E	139_P	0.510	0.68
30_I	236_N	0.510	0.68
263_T	266_G	0.508	0.68
115_Y	119_Y	0.507	0.67
23_D	26_R	0.505	0.67
243_L	250_W	0.505	0.67
134_W	171_L	0.504	0.67
22_L	26_R	0.504	0.67
96_P	99_T	0.502	0.67
148_I	226_L	0.500	0.66

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4httA	1	0.7716	100	0.175	Contact Map
4b4aA	1	0.7716	100	0.192	Contact Map
4ui9I	1	0.0415	13.3	0.963	Contact Map
2mfrA	1	0.1522	11.1	0.964	Contact Map
4p02A	1	0.173	11	0.964	Contact Map
2m7xA	1	0.1038	10.2	0.965	Contact Map
3o7qA	1	0.6782	9.3	0.966	Contact Map
2ww9B	1	0.1557	7.8	0.967	Contact Map
2cfqA	1	0.7197	7.2	0.967	Contact Map
4cbcA	3	0.0035	6.5	0.968	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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