Q97U59 - SULSO - GREMLIN Contacts

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Q97U59 - Glycolate oxidase glcE subunit. (GlcE)

UniProt:	Q97U59	Sequences: MYIGINIILLIKNYFYNLSPFLLMDWVDELGKIVKVSNGKDEGPARWKITAIPQTYAELRDVIKFANEKKLSIYPFSFNMHHIGPPVSIDIGVKLSQFNNVVEISDDDLYVTSQVGVRVSDLFEIIKAKGLFLPAYYDGSLGGLLATNLPTPFSSFYGYPKNLILMAKVITGDGIIAKGGGKTLKFSSGYKIHKILSGMLGWLGIYLEATLKIYPFPEVIVTFQTDKVNLTSKYRPISIIYETDEKGDKTYVTFIGFRRAIEKIGEEIGAKGEDGFYSIDYTSDEKVISIHTVRGKEVDVIRKAKSIMRVKRGIGIVGTGYCRLEVASFDNLEVLRREIDEQVVVERGYYNGDYWGIRDKETLQMLKEAFDPNNILQPGLIL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	382 (341)
Sequences:	18668 (14263)
Seq/√Len:	772.4

ECOLI - GLCD YGCU GLCE YDIJ
BACSU - GLCD YVDP YITY YGAK
HALSA - Q9HMR2
SULSO - Q97U59 Q97U60 Q97Y18 Q7LX43 Q97ZB9 Q7LXI5 Q97Z67

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
168_K	208_E	3.727	1.00
67_N	373_N	3.562	1.00
56_Y	168_K	3.553	1.00
145_L	209_A	3.192	1.00
100_N	114_Q	3.026	1.00
165_L	212_K	2.914	1.00
70_K	373_N	2.897	1.00
296_K	299_D	2.709	1.00
99_N	121_D	2.688	1.00
112_T	208_E	2.675	1.00
112_T	210_T	2.661	1.00
168_K	178_K	2.634	1.00
105_S	108_D	2.593	1.00
125_I	128_A	2.586	1.00
51_A	62_V	2.546	1.00
54_Q	97_Q	2.540	1.00
229_N	232_S	2.494	1.00
169_V	196_L	2.426	1.00
131_L	213_I	2.357	1.00
59_L	207_L	2.316	1.00
171_T	175_I	2.299	1.00
114_Q	208_E	2.181	1.00
60_R	176_I	2.151	1.00
166_M	178_K	2.137	1.00
65_F	69_K	2.073	1.00
117_V	122_L	2.053	1.00
127_K	216_F	2.052	1.00
166_M	210_T	2.044	1.00
63_I	73_I	1.958	1.00
120_S	124_E	1.948	1.00
172_G	376_L	1.948	1.00
101_V	117_V	1.925	1.00
106_D	129_K	1.914	1.00
334_V	338_E	1.903	1.00
38_N	41_D	1.893	1.00
118_R	121_D	1.860	1.00
39_G	42_E	1.858	1.00
56_Y	176_I	1.837	1.00
171_T	177_A	1.834	1.00
168_K	176_I	1.808	1.00
111_V	122_L	1.800	1.00
167_A	196_L	1.782	1.00
52_I	97_Q	1.775	1.00
103_E	112_T	1.770	1.00
56_Y	207_L	1.767	1.00
169_V	204_G	1.766	1.00
57_A	60_R	1.766	1.00
166_M	180_G	1.750	1.00
335_L	338_E	1.743	1.00
57_A	61_D	1.733	1.00
56_Y	60_R	1.713	1.00
59_L	205_I	1.713	1.00
33_I	36_V	1.695	1.00
60_R	64_K	1.689	1.00
98_F	114_Q	1.648	1.00
303_K	338_E	1.642	1.00
126_I	213_I	1.634	1.00
110_Y	210_T	1.633	1.00
75_P	205_I	1.616	1.00
76_F	79_N	1.616	1.00
95_L	205_I	1.604	1.00
93_V	205_I	1.604	1.00
110_Y	165_L	1.600	1.00
109_L	131_L	1.588	1.00
54_Q	58_E	1.584	1.00
55_T	58_E	1.570	1.00
125_I	129_K	1.567	1.00
231_T	302_R	1.561	1.00
61_D	64_K	1.557	1.00
368_E	374_N	1.547	1.00
266_E	270_A	1.534	1.00
63_I	376_L	1.534	1.00
163_L	214_Y	1.520	1.00
365_M	369_A	1.519	1.00
113_S	141_L	1.512	1.00
104_I	125_I	1.512	1.00
330_D	334_V	1.506	1.00
101_V	125_I	1.506	1.00
145_L	164_I	1.499	1.00
258_R	262_E	1.489	1.00
286_K	290_I	1.468	1.00
146_A	199_M	1.463	1.00
361_E	365_M	1.461	1.00
144_L	160_P	1.441	1.00
66_A	91_I	1.435	1.00
303_K	306_S	1.428	1.00
111_V	211_L	1.423	1.00
64_K	67_N	1.413	1.00
344_V	347_R	1.410	1.00
109_L	215_P	1.409	1.00
146_A	206_Y	1.402	1.00
121_D	124_E	1.389	1.00
168_K	207_L	1.386	1.00
170_I	207_L	1.381	1.00
39_G	43_G	1.379	1.00
195_I	366_L	1.372	1.00
319_T	323_R	1.360	1.00
364_Q	368_E	1.359	1.00
145_L	211_L	1.358	1.00
231_T	235_R	1.335	1.00
51_A	91_I	1.322	1.00
105_S	110_Y	1.322	1.00
245_E	249_K	1.320	1.00
95_L	98_F	1.314	1.00
328_S	331_N	1.310	1.00
310_V	331_N	1.310	1.00
343_V	346_E	1.310	1.00
361_E	364_Q	1.310	1.00
356_G	359_D	1.309	1.00
59_L	170_I	1.304	1.00
306_S	310_V	1.300	1.00
246_K	249_K	1.297	1.00
65_F	71_L	1.284	1.00
196_L	204_G	1.280	1.00
114_Q	117_V	1.279	1.00
262_E	266_E	1.278	1.00
113_S	211_L	1.250	1.00
172_G	370_F	1.240	1.00
179_G	193_H	1.239	1.00
164_I	167_A	1.222	1.00
63_I	67_N	1.215	1.00
180_G	188_S	1.210	1.00
139_G	144_L	1.210	1.00
362_T	365_M	1.208	1.00
235_R	239_I	1.204	1.00
242_E	251_Y	1.194	1.00
281_Y	285_E	1.193	1.00
176_I	207_L	1.187	1.00
59_L	93_V	1.183	1.00
342_Q	345_V	1.177	1.00
331_N	334_V	1.174	1.00
113_S	122_L	1.169	1.00
63_I	205_I	1.161	1.00
357_I	361_E	1.159	1.00
52_I	94_K	1.157	1.00
283_S	288_I	1.151	1.00
232_S	235_R	1.149	1.00
66_A	73_I	1.148	1.00
100_N	117_V	1.127	1.00
367_K	374_N	1.123	1.00
334_V	353_D	1.118	1.00
60_R	170_I	1.118	1.00
260_A	263_K	1.118	1.00
64_K	68_E	1.118	1.00
287_V	290_I	1.113	1.00
245_E	248_D	1.106	1.00
305_K	309_R	1.098	1.00
53_P	62_V	1.094	1.00
283_S	287_V	1.093	1.00
99_N	114_Q	1.091	1.00
320_G	323_R	1.086	1.00
40_K	43_G	1.082	1.00
63_I	170_I	1.078	1.00
38_N	43_G	1.073	1.00
179_G	189_G	1.068	1.00
124_E	128_A	1.068	1.00
327_A	331_N	1.067	1.00
37_S	41_D	1.062	1.00
303_K	335_L	1.061	1.00
170_I	176_I	1.058	1.00
326_V	330_D	1.052	1.00
335_L	339_I	1.051	1.00
38_N	42_E	1.044	1.00
53_P	58_E	1.044	1.00
283_S	286_K	1.037	1.00
164_I	197_S	1.035	1.00
58_E	61_D	1.032	1.00
62_V	91_I	1.030	1.00
365_M	368_E	1.026	1.00
299_D	303_K	1.017	1.00
247_G	250_T	1.014	1.00
336_R	339_I	1.014	1.00
158_G	162_N	1.013	1.00
360_K	364_Q	1.012	1.00
76_F	92_G	1.011	1.00
359_D	362_T	1.007	1.00
145_L	160_P	1.001	1.00
67_N	375_I	1.000	1.00
267_E	270_A	0.996	1.00
111_V	126_I	0.992	1.00
307_I	310_V	0.991	1.00
246_K	250_T	0.986	1.00
236_P	239_I	0.985	1.00
279_I	283_S	0.984	1.00
284_D	288_I	0.981	1.00
286_K	289_S	0.981	1.00
104_I	111_V	0.980	1.00
62_V	93_V	0.979	1.00
52_I	96_S	0.977	1.00
248_D	251_Y	0.976	1.00
111_V	213_I	0.972	1.00
329_F	333_E	0.968	1.00
331_N	335_L	0.967	1.00
343_V	347_R	0.966	1.00
260_A	264_I	0.962	1.00
277_Y	283_S	0.961	1.00
169_V	177_A	0.955	1.00
196_L	206_Y	0.955	1.00
37_S	40_K	0.954	1.00
77_S	140_S	0.952	1.00
228_V	232_S	0.951	1.00
171_T	370_F	0.948	1.00
104_I	131_L	0.946	1.00
280_D	283_S	0.945	1.00
167_A	193_H	0.937	1.00
32_K	35_K	0.934	1.00
244_D	250_T	0.933	1.00
277_Y	280_D	0.923	1.00
244_D	247_G	0.920	1.00
194_K	362_T	0.920	1.00
70_K	375_I	0.917	1.00
312_R	315_G	0.915	1.00
177_A	189_G	0.913	1.00
49_I	91_I	0.911	1.00
277_Y	290_I	0.907	1.00
141_L	211_L	0.904	1.00
73_I	93_V	0.903	1.00
345_V	348_G	0.900	1.00
30_L	34_V	0.899	1.00
121_D	125_I	0.896	1.00
95_L	115_V	0.895	1.00
142_G	209_A	0.889	1.00
245_E	250_T	0.885	1.00
344_V	349_Y	0.882	1.00
35_K	38_N	0.881	1.00
235_R	238_S	0.877	1.00
238_S	241_Y	0.876	1.00
48_K	89_I	0.872	1.00
347_R	350_Y	0.868	1.00
77_S	95_L	0.867	1.00
71_L	91_I	0.864	1.00
244_D	248_D	0.864	1.00
307_I	335_L	0.864	1.00
59_L	98_F	0.862	1.00
293_V	296_K	0.859	1.00
263_K	266_E	0.856	1.00
76_F	94_K	0.851	1.00
306_S	309_R	0.842	1.00
268_I	272_G	0.840	1.00
261_I	265_G	0.839	1.00
81_H	85_P	0.836	1.00
120_S	138_D	0.829	1.00
333_E	337_R	0.828	1.00
303_K	339_I	0.827	1.00
283_S	290_I	0.825	1.00
56_Y	170_I	0.825	1.00
61_D	65_F	0.823	1.00
167_A	209_A	0.821	1.00
234_Y	238_S	0.821	1.00
56_Y	114_Q	0.820	1.00
302_R	306_S	0.819	1.00
318_G	324_L	0.819	1.00
131_L	215_P	0.819	1.00
341_E	347_R	0.817	1.00
180_G	187_S	0.817	1.00
120_S	137_Y	0.815	1.00
164_I	209_A	0.813	1.00
298_V	302_R	0.813	1.00
344_V	348_G	0.812	1.00
181_G	187_S	0.812	1.00
119_V	144_L	0.810	1.00
72_S	377_Q	0.808	1.00
178_K	208_E	0.806	1.00
191_K	194_K	0.806	1.00
284_D	290_I	0.803	1.00
247_G	251_Y	0.799	1.00
73_I	375_I	0.799	1.00
264_I	267_E	0.799	1.00
178_K	210_T	0.794	1.00
199_M	203_L	0.792	1.00
331_N	336_R	0.792	1.00
285_E	288_I	0.791	1.00
303_K	307_I	0.788	1.00
190_Y	202_W	0.786	1.00
64_K	175_I	0.781	1.00
187_S	190_Y	0.781	1.00
242_E	246_K	0.779	1.00
346_E	350_Y	0.775	1.00
32_K	36_V	0.775	1.00
346_E	349_Y	0.775	1.00
278_S	290_I	0.774	1.00
343_V	348_G	0.774	1.00
195_I	203_L	0.773	1.00
233_K	237_I	0.773	1.00
353_D	356_G	0.770	1.00
245_E	251_Y	0.769	1.00
30_L	33_I	0.768	1.00
59_L	95_L	0.766	1.00
301_I	304_A	0.764	1.00
282_T	286_K	0.764	1.00
318_G	323_R	0.763	1.00
266_E	269_G	0.763	1.00
244_D	251_Y	0.763	1.00
252_V	255_I	0.758	1.00
78_F	140_S	0.758	1.00
72_S	90_D	0.754	1.00
277_Y	286_K	0.753	1.00
206_Y	209_A	0.753	1.00
31_G	34_V	0.748	1.00
99_N	117_V	0.746	1.00
117_V	121_D	0.742	1.00
280_D	288_I	0.738	1.00
307_I	331_N	0.736	1.00
282_T	287_V	0.734	1.00
333_E	336_R	0.734	1.00
110_Y	212_K	0.733	1.00
264_I	272_G	0.730	1.00
299_D	302_R	0.727	1.00
173_D	177_A	0.727	1.00
334_V	337_R	0.726	1.00
301_I	305_K	0.725	1.00
280_D	284_D	0.724	1.00
341_E	344_V	0.723	1.00
172_G	375_I	0.722	1.00
254_F	261_I	0.721	1.00
244_D	249_K	0.720	1.00
130_G	216_F	0.718	1.00
304_A	307_I	0.717	1.00
96_S	140_S	0.715	1.00
237_I	241_Y	0.713	1.00
36_V	39_G	0.712	1.00
331_N	339_I	0.709	1.00
302_R	305_K	0.707	1.00
265_G	269_G	0.707	1.00
34_V	38_N	0.707	1.00
163_L	212_K	0.705	1.00
300_V	304_A	0.705	1.00
53_P	95_L	0.702	1.00
341_E	345_V	0.701	1.00
279_I	287_V	0.701	1.00
115_V	209_A	0.700	1.00
75_P	95_L	0.699	1.00
53_P	97_Q	0.697	1.00
278_S	283_S	0.695	1.00
325_E	329_F	0.693	1.00
296_K	339_I	0.691	1.00
191_K	369_A	0.690	1.00
281_Y	284_D	0.689	1.00
179_G	196_L	0.688	1.00
237_I	240_I	0.687	1.00
63_I	172_G	0.681	1.00
292_T	300_V	0.679	1.00
313_G	316_I	0.678	1.00
301_I	310_V	0.677	1.00
136_Y	139_G	0.676	1.00
134_P	163_L	0.674	1.00
132_F	216_F	0.673	1.00
341_E	346_E	0.671	1.00
254_F	268_I	0.670	1.00
67_N	173_D	0.669	1.00
355_W	359_D	0.669	1.00
312_R	316_I	0.668	1.00
327_A	330_D	0.668	1.00
280_D	289_S	0.667	1.00
282_T	285_E	0.666	1.00
56_Y	59_L	0.664	1.00
253_T	256_G	0.664	1.00
280_D	287_V	0.664	1.00
263_K	267_E	0.662	1.00
342_Q	347_R	0.662	1.00
242_E	245_E	0.661	1.00
284_D	289_S	0.660	1.00
134_P	214_Y	0.659	1.00
325_E	328_S	0.658	1.00
343_V	349_Y	0.657	1.00
105_S	112_T	0.656	1.00
267_E	271_K	0.654	1.00
167_A	206_Y	0.654	1.00
304_A	308_M	0.653	1.00
257_F	260_A	0.653	1.00
165_L	210_T	0.652	1.00
98_F	207_L	0.649	1.00
141_L	209_A	0.649	1.00
48_K	90_D	0.648	1.00
291_H	294_R	0.648	1.00
50_T	92_G	0.646	1.00
111_V	125_I	0.646	1.00
326_V	329_F	0.644	1.00
77_S	115_V	0.643	1.00
374_N	377_Q	0.643	1.00
159_Y	162_N	0.643	1.00
305_K	310_V	0.642	1.00
242_E	249_K	0.639	1.00
342_Q	346_E	0.637	1.00
96_S	118_R	0.636	1.00
28_D	31_G	0.630	1.00
47_W	92_G	0.630	1.00
279_I	282_T	0.629	1.00
328_S	336_R	0.629	1.00
193_H	197_S	0.627	1.00
269_G	272_G	0.627	1.00
119_V	139_G	0.626	1.00
37_S	42_E	0.626	1.00
291_H	342_Q	0.625	1.00
332_L	336_R	0.624	1.00
330_D	333_E	0.618	1.00
329_F	332_L	0.617	1.00
167_A	179_G	0.616	1.00
360_K	363_L	0.616	1.00
122_L	141_L	0.615	1.00
56_Y	98_F	0.615	1.00
328_S	332_L	0.614	1.00
299_D	339_I	0.612	1.00
227_K	236_P	0.609	1.00
345_V	349_Y	0.607	1.00
281_Y	287_V	0.606	1.00
124_E	127_K	0.602	1.00
300_V	339_I	0.602	1.00
203_L	366_L	0.601	1.00
221_V	262_E	0.601	1.00
366_L	370_F	0.600	1.00
278_S	284_D	0.599	1.00
254_F	265_G	0.595	1.00
312_R	317_V	0.595	1.00
147_T	345_V	0.594	1.00
166_M	208_E	0.594	1.00
279_I	284_D	0.593	1.00
108_D	295_G	0.591	1.00
367_K	377_Q	0.588	1.00
101_V	114_Q	0.586	1.00
285_E	289_S	0.586	1.00
142_G	146_A	0.586	1.00
259_R	263_K	0.585	1.00
36_V	41_D	0.583	1.00
278_S	282_T	0.582	1.00
279_I	288_I	0.581	1.00
242_E	250_T	0.580	1.00
119_V	122_L	0.579	1.00
305_K	308_M	0.579	1.00
281_Y	288_I	0.578	1.00
56_Y	208_E	0.577	1.00
294_R	301_I	0.576	1.00
28_D	34_V	0.574	1.00
30_L	35_K	0.573	1.00
145_L	206_Y	0.573	1.00
57_A	176_I	0.569	1.00
361_E	368_E	0.569	1.00
328_S	333_E	0.569	1.00
303_K	310_V	0.568	1.00
58_E	62_V	0.566	1.00
246_K	251_Y	0.565	1.00
277_Y	282_T	0.565	1.00
123_F	216_F	0.564	1.00
292_T	295_G	0.559	1.00
278_S	287_V	0.557	1.00
278_S	281_Y	0.555	1.00
191_K	366_L	0.554	1.00
279_I	290_I	0.552	1.00
34_V	40_K	0.551	1.00
53_P	59_L	0.549	1.00
106_D	109_L	0.549	1.00
123_F	132_F	0.548	1.00
278_S	288_I	0.545	1.00
126_I	129_K	0.545	1.00
313_G	317_V	0.543	1.00
300_V	303_K	0.541	1.00
318_G	322_C	0.538	1.00
328_S	335_L	0.537	1.00
120_S	136_Y	0.535	1.00
280_D	286_K	0.535	1.00
53_P	98_F	0.534	1.00
273_E	277_Y	0.533	1.00
281_Y	286_K	0.533	1.00
234_Y	237_I	0.533	1.00
236_P	241_Y	0.532	1.00
122_L	133_L	0.531	1.00
277_Y	284_D	0.529	1.00
345_V	350_Y	0.529	1.00
314_I	317_V	0.529	1.00
60_R	63_I	0.529	1.00
285_E	290_I	0.527	1.00
154_S	159_Y	0.524	1.00
342_Q	348_G	0.524	1.00
104_I	129_K	0.523	1.00
333_E	339_I	0.523	1.00
261_I	268_I	0.523	1.00
340_D	346_E	0.522	1.00
259_R	262_E	0.520	1.00
294_R	298_V	0.520	1.00
280_D	290_I	0.518	1.00
295_G	298_V	0.518	1.00
277_Y	287_V	0.517	1.00
254_F	257_F	0.517	1.00
168_K	210_T	0.516	1.00
358_R	361_E	0.515	1.00
178_K	188_S	0.515	1.00
321_Y	324_L	0.515	1.00
74_Y	90_D	0.515	1.00
328_S	339_I	0.512	1.00
105_S	109_L	0.510	1.00
228_V	231_T	0.509	1.00
189_G	370_F	0.509	1.00
292_T	298_V	0.508	1.00
72_S	378_P	0.508	1.00
101_V	113_S	0.508	1.00
281_Y	289_S	0.507	1.00
221_V	265_G	0.507	1.00
34_V	42_E	0.507	1.00
320_G	324_L	0.506	1.00
78_F	118_R	0.505	1.00
180_G	189_G	0.505	1.00
202_W	367_K	0.504	1.00
148_N	159_Y	0.504	1.00
277_Y	289_S	0.504	1.00
33_I	37_S	0.503	1.00
31_G	35_K	0.502	1.00
65_F	91_I	0.502	1.00
165_L	181_G	0.501	1.00
151_T	154_S	0.501	1.00
283_S	289_S	0.500	1.00
233_K	238_S	0.500	1.00
169_V	179_G	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3pm9A	5	0.9791	100	0.276	Contact Map
2uuuA	2	0.9555	100	0.313	Contact Map
1f0xA	1	0.9503	100	0.336	Contact Map
4bbyA	2	0.9948	100	0.347	Contact Map
1w1oA	1	0.9241	100	0.352	Contact Map
4o95A	1	0.9267	100	0.36	Contact Map
4xloA	2	0.9686	100	0.362	Contact Map
4mlaA	1	0.9162	100	0.366	Contact Map
1wvfA	2	0.9791	100	0.368	Contact Map
2exrA	1	0.9136	100	0.37	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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