Q97U51 - SULSO - GREMLIN Contacts

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Q97U51 - Second ORF in transposon ISC1904

UniProt:	Q97U51	Sequences: MLKNLRIRKFEPEEEYVHFTYSIKNSEREKSKELIKEYRTLLQKAIDYLWNLTKIQVRKKNGSYKITLPKKKEVYKPLREELEKINHLASHYVDKAINDAFSILKSWRKRAIKGRASIEKPTLKKAYVRIKTTLRKVVGESVRITVRPYEYITFSWSKSWFSRRVRELELGEPIIKEEKVYLPFRYKLPWVTPVNFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRGRELMRKYSHRERNRVLDFVHKFVNTLLDLYPMTFFAVEKLNKESMFKDANGSLSRKISRTVWRSIHRVLKYKAPLYGSFVKEVNPHLTSRSCPRCGFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIRDIPRVWVGVIPLMGRRGMNVRDFGEAQGLRIDIKYHEIL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	422 (343)
Sequences:	4356 (2762)
Seq/√Len:	149.1

ECOLI - INSQ
HALSA - Q9HSW9 Q9HHJ9 Q9HM87 Q9HSW7 Q9HHG8 Q9HSZ6 Q9HSD4 Q9HHU6 Q9HSY4 Q9HHX8
SULSO - Q97U51 Q97XQ0 Q97YW9 Q97Z61 Q97YI5 Q97YU1 Q97UQ8 Q97U68 Q97XB6 Q97Y48 Q97XQ8 Q97YI2 Q97WZ3 Q97V47 Q97XP1 Q97V25 Q97Y44 Q97X39 Q97Y67 Q97YW4 Q7LXH2 Q97V53 Q7LXD9 Q97XA4 Q97WY6 Q7LXH6 Q97YF7 Y2105 Q97U09 Q7LX59

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
264_D	268_K	5.296	1.00
288_N	291_S	4.320	1.00
340_P	367_N	4.131	1.00
320_A	327_V	3.689	1.00
206_L	312_I	2.990	1.00
313_H	329_E	2.966	1.00
284_V	316_L	2.940	1.00
283_A	376_M	2.914	1.00
19_F	322_L	2.874	1.00
347_R	350_G	2.828	1.00
311_S	315_V	2.775	1.00
271_N	275_D	2.730	1.00
312_I	316_L	2.434	1.00
338_S	345_V	2.425	1.00
311_S	314_R	2.393	1.00
226_K	302_R	2.373	1.00
334_L	337_R	2.324	1.00
317_K	329_E	2.323	1.00
173_P	182_L	2.323	1.00
330_V	372_I	2.254	1.00
274_L	320_A	2.253	1.00
293_F	305_S	2.210	1.00
375_K	378_G	2.148	1.00
282_F	320_A	2.116	1.00
171_G	187_K	2.113	1.00
54_K	120_K	2.105	1.00
225_L	261_R	2.105	1.00
337_R	346_S	2.103	1.00
267_H	315_V	2.080	1.00
335_T	372_I	2.068	1.00
284_V	327_V	2.003	1.00
28_R	31_S	1.994	1.00
354_E	361_K	1.991	1.00
232_K	247_G	1.990	1.00
51_N	55_I	1.986	1.00
267_H	318_Y	1.973	1.00
263_L	311_S	1.946	1.00
342_C	358_C	1.931	1.00
36_K	180_V	1.899	1.00
49_L	97_I	1.878	1.00
270_V	315_V	1.875	1.00
206_L	265_F	1.874	1.00
298_G	301_S	1.856	1.00
341_R	360_F	1.839	1.00
266_V	315_V	1.821	1.00
351_K	364_R	1.767	1.00
342_C	355_C	1.766	1.00
37_E	87_H	1.760	1.00
353_F	362_L	1.754	1.00
339_C	342_C	1.748	1.00
242_K	245_K	1.741	1.00
317_K	321_P	1.734	1.00
247_G	251_M	1.730	1.00
50_W	100_A	1.729	1.00
355_C	358_C	1.718	1.00
339_C	358_C	1.715	1.00
267_H	319_K	1.697	1.00
267_H	270_V	1.679	1.00
245_K	248_R	1.631	1.00
284_V	329_E	1.631	1.00
266_V	311_S	1.623	1.00
232_K	235_K	1.611	1.00
35_I	180_V	1.596	1.00
44_K	84_K	1.595	1.00
144_I	182_L	1.584	1.00
20_T	183_P	1.584	1.00
352_T	361_K	1.550	1.00
33_E	37_E	1.548	1.00
339_C	355_C	1.540	1.00
199_I	207_D	1.522	1.00
286_K	329_E	1.517	1.00
278_P	325_S	1.516	1.00
284_V	313_H	1.514	1.00
208_A	366_L	1.505	1.00
346_S	350_G	1.488	1.00
177_E	256_H	1.488	1.00
344_F	356_E	1.482	1.00
196_F	373_Y	1.476	1.00
68_L	72_K	1.475	1.00
69_P	72_K	1.471	1.00
106_S	118_I	1.438	1.00
39_R	180_V	1.414	1.00
205_T	208_A	1.412	1.00
101_F	105_K	1.409	1.00
353_F	360_F	1.409	1.00
110_R	118_I	1.404	1.00
32_K	36_K	1.402	1.00
111_A	118_I	1.384	1.00
236_V	248_R	1.366	1.00
49_L	100_A	1.366	1.00
179_K	256_H	1.365	1.00
270_V	318_Y	1.362	1.00
348_K	351_K	1.357	1.00
240_A	244_T	1.352	1.00
145_T	151_Y	1.351	1.00
136_K	145_T	1.338	1.00
209_Y	213_K	1.336	1.00
317_K	327_V	1.333	1.00
270_V	319_K	1.324	1.00
232_K	254_Y	1.323	1.00
285_E	332_P	1.321	1.00
197_L	209_Y	1.320	1.00
295_D	301_S	1.304	1.00
370_L	374_L	1.301	1.00
256_H	260_N	1.298	1.00
304_I	309_W	1.288	1.00
105_K	109_K	1.275	1.00
196_F	281_F	1.263	1.00
24_K	179_K	1.262	1.00
91_H	131_K	1.231	1.00
104_L	120_K	1.225	1.00
68_L	98_N	1.220	1.00
102_S	106_S	1.215	1.00
66_I	71_K	1.202	1.00
272_T	276_L	1.196	1.00
54_K	58_R	1.192	1.00
29_E	32_K	1.191	1.00
229_M	255_S	1.185	1.00
239_F	247_G	1.184	1.00
197_L	277_Y	1.172	1.00
366_L	370_L	1.168	1.00
340_P	370_L	1.165	1.00
274_L	323_Y	1.164	1.00
99_D	129_R	1.157	1.00
229_M	251_M	1.152	1.00
27_E	31_S	1.151	1.00
225_L	258_E	1.149	1.00
91_H	133_T	1.148	1.00
340_P	362_L	1.146	1.00
95_K	98_N	1.133	1.00
199_I	209_Y	1.128	0.99
69_P	73_E	1.128	0.99
288_N	332_P	1.128	0.99
338_S	375_K	1.122	0.99
362_L	370_L	1.116	0.99
266_V	312_I	1.116	0.99
201_S	309_W	1.109	0.99
70_K	73_E	1.107	0.99
76_K	79_R	1.105	0.99
263_L	315_V	1.102	0.99
55_I	59_K	1.101	0.99
270_V	316_L	1.098	0.99
42_L	96_A	1.091	0.99
259_R	263_L	1.090	0.99
233_R	237_Q	1.089	0.99
203_L	307_T	1.088	0.99
347_R	351_K	1.079	0.99
228_S	232_K	1.071	0.99
270_V	312_I	1.066	0.99
198_A	373_Y	1.060	0.99
348_K	364_R	1.057	0.99
214_F	363_D	1.054	0.99
233_R	251_M	1.053	0.99
235_K	239_F	1.048	0.99
275_D	323_Y	1.042	0.99
89_A	92_Y	1.042	0.99
134_L	147_R	1.041	0.99
77_P	81_E	1.039	0.99
271_N	323_Y	1.038	0.99
315_V	318_Y	1.038	0.99
319_K	322_L	1.037	0.99
156_W	159_S	1.037	0.99
128_V	132_T	1.037	0.99
335_T	375_K	1.037	0.99
110_R	119_E	1.031	0.99
128_V	131_K	1.025	0.99
27_E	35_I	1.024	0.99
146_V	175_I	1.020	0.99
106_S	111_A	1.019	0.99
52_L	74_V	1.015	0.99
106_S	110_R	1.014	0.99
201_S	313_H	1.013	0.99
274_L	325_S	1.012	0.99
266_V	270_V	1.010	0.99
236_V	251_M	1.009	0.99
21_Y	186_Y	1.006	0.99
344_F	357_R	1.001	0.99
46_I	96_A	0.991	0.99
232_K	250_L	0.989	0.99
337_R	345_V	0.985	0.99
289_K	310_R	0.983	0.99
347_R	364_R	0.980	0.99
362_L	366_L	0.979	0.99
79_R	93_V	0.973	0.98
196_F	377_C	0.971	0.98
205_T	216_T	0.971	0.98
286_K	332_P	0.968	0.98
41_L	84_K	0.968	0.98
330_V	375_K	0.968	0.98
208_A	214_F	0.967	0.98
274_L	282_F	0.964	0.98
54_K	104_L	0.948	0.98
295_D	298_G	0.948	0.98
330_V	376_M	0.947	0.98
50_W	104_L	0.946	0.98
32_K	180_V	0.946	0.98
295_D	299_S	0.945	0.98
341_R	370_L	0.945	0.98
106_S	109_K	0.936	0.98
351_K	363_D	0.936	0.98
281_F	326_F	0.933	0.98
232_K	251_M	0.927	0.98
273_L	282_F	0.927	0.98
193_P	278_P	0.924	0.98
244_T	248_R	0.922	0.98
366_L	369_S	0.921	0.98
218_S	307_T	0.919	0.98
44_K	48_Y	0.916	0.98
197_L	210_D	0.910	0.98
95_K	129_R	0.906	0.98
283_A	372_I	0.906	0.98
346_S	353_F	0.903	0.98
281_F	328_K	0.900	0.97
91_H	95_K	0.896	0.97
138_V	141_S	0.895	0.97
207_D	269_F	0.895	0.97
320_A	325_S	0.892	0.97
350_G	364_R	0.891	0.97
75_Y	79_R	0.889	0.97
229_M	258_E	0.887	0.97
52_L	56_Q	0.882	0.97
107_W	121_P	0.879	0.97
340_P	360_F	0.878	0.97
221_Q	261_R	0.877	0.97
314_R	317_K	0.873	0.97
352_T	362_L	0.869	0.97
332_P	335_T	0.866	0.97
46_I	103_I	0.865	0.97
266_V	308_V	0.863	0.97
154_F	158_K	0.863	0.97
67_T	70_K	0.862	0.97
334_L	375_K	0.860	0.97
196_F	283_A	0.860	0.97
40_T	43_Q	0.857	0.97
68_L	97_I	0.855	0.97
230_E	234_A	0.853	0.96
250_L	253_K	0.850	0.96
18_H	185_R	0.850	0.96
257_R	261_R	0.848	0.96
73_E	76_K	0.844	0.96
46_I	100_A	0.844	0.96
230_E	233_R	0.841	0.96
99_D	174_I	0.840	0.96
85_I	93_V	0.833	0.96
129_R	172_E	0.832	0.96
236_V	247_G	0.829	0.96
71_K	74_V	0.829	0.96
56_Q	60_K	0.828	0.96
143_R	151_Y	0.828	0.96
229_M	237_Q	0.823	0.96
271_N	319_K	0.819	0.96
254_Y	258_E	0.819	0.96
241_S	244_T	0.815	0.95
130_I	146_V	0.813	0.95
18_H	187_K	0.812	0.95
201_S	207_D	0.812	0.95
25_N	182_L	0.811	0.95
94_D	98_N	0.811	0.95
95_K	99_D	0.809	0.95
107_W	120_K	0.809	0.95
265_F	269_F	0.804	0.95
213_K	272_T	0.804	0.95
136_K	139_G	0.800	0.95
53_T	101_F	0.798	0.95
203_L	303_K	0.797	0.95
57_V	61_N	0.794	0.95
91_H	132_T	0.793	0.95
72_K	97_I	0.792	0.95
48_Y	52_L	0.791	0.95
289_K	309_W	0.790	0.95
221_Q	225_L	0.790	0.95
367_N	371_N	0.789	0.95
291_S	332_P	0.789	0.95
248_R	252_R	0.788	0.95
229_M	233_R	0.786	0.94
233_R	255_S	0.783	0.94
368_A	371_N	0.782	0.94
269_F	272_T	0.780	0.94
72_K	76_K	0.777	0.94
300_L	304_I	0.775	0.94
293_F	301_S	0.774	0.94
24_K	181_Y	0.773	0.94
174_I	183_P	0.773	0.94
370_L	373_Y	0.772	0.94
328_K	376_M	0.769	0.94
175_I	182_L	0.766	0.94
344_F	353_F	0.765	0.94
338_S	371_N	0.764	0.94
350_G	353_F	0.762	0.94
41_L	88_L	0.762	0.94
197_L	282_F	0.759	0.93
154_F	157_S	0.756	0.93
88_L	91_H	0.755	0.93
230_E	237_Q	0.754	0.93
215_V	265_F	0.753	0.93
340_P	374_L	0.753	0.93
205_T	214_F	0.751	0.93
340_P	368_A	0.747	0.93
65_K	70_K	0.747	0.93
129_R	132_T	0.743	0.93
192_T	195_N	0.742	0.93
23_I	182_L	0.741	0.93
134_L	137_V	0.738	0.92
45_A	96_A	0.737	0.92
53_T	71_K	0.733	0.92
334_L	338_S	0.731	0.92
214_F	366_L	0.727	0.92
95_K	131_K	0.724	0.92
34_L	38_Y	0.724	0.92
282_F	316_L	0.723	0.92
91_H	94_D	0.723	0.92
61_N	65_K	0.721	0.92
40_T	44_K	0.720	0.91
204_Y	208_A	0.717	0.91
375_K	379_F	0.715	0.91
44_K	82_L	0.715	0.91
353_F	364_R	0.712	0.91
283_A	373_Y	0.711	0.91
231_V	235_K	0.708	0.91
29_E	33_E	0.704	0.91
53_T	104_L	0.701	0.90
291_S	304_I	0.700	0.90
57_V	104_L	0.698	0.90
262_V	306_R	0.698	0.90
200_D	365_Q	0.695	0.90
286_K	313_H	0.693	0.90
202_N	208_A	0.692	0.90
232_K	236_V	0.689	0.90
287_L	332_P	0.686	0.89
236_V	244_T	0.683	0.89
38_Y	42_L	0.682	0.89
91_H	134_L	0.682	0.89
129_R	174_I	0.681	0.89
76_K	80_E	0.681	0.89
258_E	306_R	0.676	0.89
97_I	101_F	0.674	0.89
71_K	75_Y	0.673	0.89
206_L	307_T	0.673	0.89
283_A	328_K	0.671	0.88
169_E	187_K	0.670	0.88
334_L	345_V	0.670	0.88
20_T	185_R	0.668	0.88
138_V	145_T	0.667	0.88
225_L	257_R	0.666	0.88
96_A	100_A	0.665	0.88
104_L	108_R	0.665	0.88
377_C	380_P	0.664	0.88
127_Y	131_K	0.660	0.88
346_S	364_R	0.660	0.88
135_R	170_L	0.660	0.88
156_W	160_W	0.659	0.87
75_Y	97_I	0.658	0.87
18_H	171_G	0.658	0.87
190_W	325_S	0.657	0.87
131_K	134_L	0.657	0.87
66_I	70_K	0.657	0.87
41_L	44_K	0.655	0.87
60_K	65_K	0.655	0.87
144_I	173_P	0.654	0.87
133_T	136_K	0.654	0.87
310_R	314_R	0.652	0.87
76_K	97_I	0.652	0.87
376_M	380_P	0.651	0.87
35_I	39_R	0.650	0.87
30_K	33_E	0.649	0.87
210_D	216_T	0.647	0.86
23_I	158_K	0.647	0.86
235_K	247_G	0.645	0.86
263_L	267_H	0.644	0.86
172_E	185_R	0.644	0.86
209_Y	215_V	0.643	0.86
295_D	304_I	0.642	0.86
218_S	221_Q	0.642	0.86
237_Q	251_M	0.642	0.86
146_V	149_Y	0.641	0.86
346_S	351_K	0.639	0.86
27_E	32_K	0.638	0.86
66_I	74_V	0.637	0.86
26_S	155_S	0.635	0.85
100_A	104_L	0.635	0.85
206_L	270_V	0.634	0.85
157_S	160_W	0.634	0.85
154_F	159_S	0.633	0.85
149_Y	152_I	0.631	0.85
341_R	362_L	0.628	0.85
17_V	187_K	0.628	0.85
273_L	277_Y	0.628	0.85
92_Y	128_V	0.627	0.85
275_D	281_F	0.626	0.85
207_D	312_I	0.626	0.85
37_E	41_L	0.625	0.85
234_A	238_S	0.621	0.84
52_L	78_L	0.619	0.84
93_V	96_A	0.619	0.84
48_Y	78_L	0.619	0.84
41_L	85_I	0.618	0.84
195_N	211_G	0.615	0.84
67_T	71_K	0.615	0.84
225_L	229_M	0.614	0.84
90_S	131_K	0.613	0.83
313_H	317_K	0.610	0.83
200_D	336_S	0.609	0.83
75_Y	78_L	0.608	0.83
190_W	193_P	0.605	0.83
127_Y	130_I	0.604	0.83
107_W	110_R	0.601	0.82
316_L	327_V	0.601	0.82
104_L	107_W	0.600	0.82
204_Y	351_K	0.600	0.82
88_L	93_V	0.598	0.82
338_S	343_G	0.598	0.82
22_S	183_P	0.598	0.82
103_I	121_P	0.597	0.82
52_L	55_I	0.595	0.82
255_S	259_R	0.595	0.82
283_A	375_K	0.594	0.81
203_L	294_K	0.594	0.81
195_N	277_Y	0.594	0.81
362_L	367_N	0.593	0.81
74_V	77_P	0.592	0.81
338_S	353_F	0.591	0.81
350_G	356_E	0.589	0.81
298_G	305_S	0.588	0.81
225_L	254_Y	0.587	0.81
38_Y	88_L	0.582	0.80
356_E	364_R	0.582	0.80
374_L	379_F	0.581	0.80
318_Y	322_L	0.579	0.80
203_L	293_F	0.579	0.80
75_Y	94_D	0.577	0.80
372_I	375_K	0.577	0.80
109_K	118_I	0.576	0.79
201_S	284_V	0.573	0.79
34_L	37_E	0.573	0.79
51_N	123_L	0.572	0.79
331_N	334_L	0.571	0.79
39_R	128_V	0.571	0.79
244_T	247_G	0.570	0.79
38_Y	89_A	0.568	0.79
245_K	249_E	0.566	0.78
76_K	81_E	0.566	0.78
222_L	225_L	0.563	0.78
244_T	251_M	0.563	0.78
51_N	54_K	0.561	0.78
68_L	76_K	0.561	0.78
49_L	74_V	0.561	0.78
25_N	154_F	0.560	0.78
255_S	262_V	0.557	0.77
348_K	352_T	0.557	0.77
320_A	323_Y	0.557	0.77
74_V	78_L	0.556	0.77
289_K	292_M	0.555	0.77
145_T	148_P	0.553	0.77
235_K	250_L	0.551	0.76
123_L	126_A	0.550	0.76
206_L	266_V	0.548	0.76
21_Y	158_K	0.548	0.76
138_V	143_R	0.548	0.76
208_A	216_T	0.547	0.76
347_R	363_D	0.547	0.76
310_R	313_H	0.546	0.76
72_K	98_N	0.545	0.76
289_K	304_I	0.544	0.76
57_V	108_R	0.544	0.76
41_L	93_V	0.540	0.75
96_A	99_D	0.539	0.75
223_Y	302_R	0.535	0.74
38_Y	130_I	0.534	0.74
283_A	377_C	0.534	0.74
269_F	312_I	0.531	0.74
68_L	71_K	0.528	0.73
92_Y	130_I	0.528	0.73
176_K	179_K	0.528	0.73
179_K	260_N	0.528	0.73
65_K	71_K	0.528	0.73
90_S	134_L	0.528	0.73
137_V	141_S	0.526	0.73
57_V	107_W	0.526	0.73
74_V	79_R	0.525	0.73
140_E	143_R	0.524	0.73
260_N	263_L	0.524	0.73
22_S	263_L	0.523	0.73
348_K	363_D	0.523	0.73
56_Q	59_K	0.521	0.72
315_V	319_K	0.519	0.72
277_Y	282_F	0.518	0.72
90_S	133_T	0.517	0.72
266_V	269_F	0.517	0.72
39_R	42_L	0.517	0.72
70_K	75_Y	0.516	0.72
292_M	304_I	0.515	0.72
97_I	100_A	0.515	0.72
235_K	238_S	0.515	0.72
27_E	152_I	0.515	0.72
49_L	78_L	0.514	0.71
271_N	274_L	0.514	0.71
68_L	75_Y	0.514	0.71
300_L	303_K	0.514	0.71
333_H	337_R	0.513	0.71
198_A	283_A	0.513	0.71
181_Y	256_H	0.512	0.71
228_S	254_Y	0.511	0.71
48_Y	84_K	0.510	0.71
53_T	57_V	0.509	0.71
230_E	238_S	0.507	0.70
53_T	56_Q	0.504	0.70
230_E	298_G	0.503	0.70
37_E	40_T	0.502	0.70
223_Y	226_K	0.502	0.70
145_T	149_Y	0.501	0.69
236_V	239_F	0.501	0.69
239_F	243_H	0.500	0.69
248_R	251_M	0.500	0.69
363_D	368_A	0.500	0.69

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1nu0A	1	0.3081	95.4	0.932	Contact Map
1vhxA	2	0.3246	94.3	0.936	Contact Map
1iv0A	1	0.2299	92.4	0.94	Contact Map
1qypA	1	0.1019	86.4	0.947	Contact Map
4aybP	1	0.0948	83.4	0.949	Contact Map
2k4xA	1	0.1256	78.4	0.952	Contact Map
1ffkW	1	0.1659	77.8	0.952	Contact Map
3j20Y	1	0.1137	76.9	0.952	Contact Map
1vd4A	1	0.1256	72.7	0.954	Contact Map
3j3w0	1	0.1303	65.7	0.956	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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