Q97U32 - SULSO - GREMLIN Contacts

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Q97U32 - Formate dehydrogenase (Amino end)

UniProt:	Q97U32	Sequences: MVHACTRDCYDTCIFDDNHKPIDTFPFNGFTCSRGIADLKRNSLNRVDSVYIEGKLSNIDEALDLIVKEIRKRKKEEILHVDYDGNQGLLTWYYPARLWNVIGAASTDYSICSSEGHEAIKLHYGNSIGALPEDFLKYESFVIWGSELVFSFIHGWNLIRDKYKATIDVRISETAKRSNKYYIVKPGSDVYLAIGIIKRLFERNWADLSLLDEPEELRNYVYSFNDEEIEEATGLDSSKIDELAELYYYRRPLTIIGFALGRSINGGDAISLISLIPALIGMKKGFFYANSQGLGIDFKYLPRTSQILSFKNCRYAEVGKEIEEGKITFMFVWNSNPLHSLPMSDRIYDAVKEVRLFLVVHDPYCQKLLR	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	370 (362)
Sequences:	9115 (5257)
Seq/√Len:	276.3

ECOLI - YDEP FDHF NAPA BISC DMSA TORZ YNFE YNFF TORA FDOG FDNG NUOG
BACSU - NASC YYAE YOAE YJGC FDHL
HALSA - DMSA
SULSO - Q97U32 Q97ZA1 Q97V13 Q97XX8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
49_S	56_L	5.891	1.00
203_R	245_E	3.158	1.00
197_I	217_L	3.008	1.00
144_W	193_A	2.999	1.00
195_G	229_I	2.996	1.00
199_R	203_R	2.982	1.00
227_E	237_S	2.947	1.00
198_K	202_E	2.900	1.00
208_L	212_D	2.868	1.00
52_I	65_L	2.779	1.00
58_N	61_E	2.758	1.00
197_I	279_L	2.699	1.00
180_K	242_E	2.684	1.00
230_E	237_S	2.668	1.00
192_L	233_T	2.573	1.00
237_S	241_D	2.572	1.00
52_I	57_S	2.567	1.00
166_T	178_S	2.485	1.00
139_E	252_P	2.411	1.00
239_K	242_E	2.407	1.00
225_N	228_E	2.385	1.00
236_D	239_K	2.384	1.00
140_S	246_L	2.325	1.00
206_A	211_L	2.308	1.00
70_I	329_F	2.305	1.00
204_N	208_L	2.290	1.00
328_T	355_R	2.272	1.00
331_F	361_H	2.197	1.00
66_I	359_V	2.194	1.00
180_K	239_K	2.162	1.00
242_E	246_L	2.161	1.00
182_Y	243_L	2.145	1.00
137_K	283_K	2.134	1.00
65_L	69_E	2.121	1.00
138_Y	141_F	2.038	1.00
209_S	213_E	2.030	1.00
57_S	65_L	2.025	1.00
140_S	165_A	2.005	1.00
182_Y	235_L	1.933	1.00
192_L	240_I	1.919	1.00
201_F	218_R	1.891	1.00
198_K	226_D	1.890	1.00
77_E	328_T	1.888	1.00
199_R	241_D	1.857	1.00
180_K	246_L	1.847	1.00
52_I	55_K	1.817	1.00
356_L	359_V	1.815	1.00
50_V	59_I	1.794	1.00
73_R	328_T	1.786	1.00
205_W	248_Y	1.783	1.00
348_Y	352_K	1.770	1.00
200_L	248_Y	1.752	1.00
202_E	222_Y	1.732	1.00
320_K	346_R	1.725	1.00
247_Y	280_I	1.714	1.00
139_E	250_R	1.711	1.00
203_R	241_D	1.693	1.00
245_E	249_Y	1.684	1.00
188_S	232_A	1.665	1.00
163_Y	179_N	1.664	1.00
219_N	222_Y	1.655	1.00
167_I	192_L	1.650	1.00
354_V	358_L	1.648	1.00
229_I	240_I	1.645	1.00
64_D	68_K	1.635	1.00
199_R	245_E	1.616	1.00
200_L	206_A	1.592	1.00
349_D	352_K	1.589	1.00
80_H	99_W	1.582	1.00
323_E	346_R	1.580	1.00
218_R	222_Y	1.575	1.00
199_R	226_D	1.569	1.00
180_K	243_L	1.562	1.00
140_S	243_L	1.535	1.00
57_S	61_E	1.531	1.00
188_S	233_T	1.528	1.00
142_V	243_L	1.524	1.00
247_Y	253_L	1.516	1.00
74_K	77_E	1.514	1.00
199_R	202_E	1.512	1.00
216_E	219_N	1.504	1.00
61_E	65_L	1.503	1.00
230_E	236_D	1.498	1.00
14_I	25_F	1.495	1.00
205_W	245_E	1.489	1.00
99_W	104_A	1.482	1.00
169_V	181_Y	1.482	1.00
191_Y	232_A	1.479	1.00
230_E	240_I	1.474	1.00
196_I	243_L	1.470	1.00
142_V	167_I	1.465	1.00
79_L	327_I	1.436	1.00
201_F	221_V	1.430	1.00
166_T	175_A	1.404	1.00
51_Y	56_L	1.395	1.00
209_S	212_D	1.387	1.00
227_E	241_D	1.377	1.00
332_V	358_L	1.377	1.00
182_Y	239_K	1.361	1.00
224_F	229_I	1.361	1.00
139_E	251_R	1.349	1.00
201_F	206_A	1.347	1.00
224_F	228_E	1.341	1.00
65_L	356_L	1.333	1.00
198_K	222_Y	1.327	1.00
140_S	250_R	1.320	1.00
192_L	243_L	1.314	1.00
217_L	221_V	1.314	1.00
317_E	320_K	1.308	1.00
79_L	318_V	1.295	1.00
260_L	273_I	1.286	1.00
70_I	359_V	1.284	1.00
228_E	231_E	1.279	1.00
134_D	137_K	1.277	1.00
224_F	232_A	1.271	1.00
163_Y	177_R	1.267	1.00
196_I	244_A	1.261	1.00
184_V	188_S	1.245	1.00
160_R	163_Y	1.239	1.00
202_E	241_D	1.225	1.00
250_R	253_L	1.222	1.00
338_L	344_S	1.222	1.00
69_E	355_R	1.219	1.00
200_L	280_I	1.213	1.00
171_I	176_K	1.206	1.00
196_I	247_Y	1.203	1.00
329_F	356_L	1.198	1.00
141_F	162_K	1.195	1.00
197_I	221_V	1.190	1.00
343_M	346_R	1.190	1.00
223_S	228_E	1.189	1.00
329_F	359_V	1.189	1.00
78_I	329_F	1.187	1.00
321_E	326_K	1.180	1.00
318_V	327_I	1.174	1.00
322_I	354_V	1.170	1.00
205_W	249_Y	1.167	1.00
193_A	273_I	1.156	1.00
282_M	285_G	1.156	1.00
142_V	196_I	1.155	1.00
143_I	146_S	1.153	1.00
144_W	167_I	1.153	1.00
119_A	270_I	1.148	1.00
66_I	356_L	1.146	1.00
73_R	77_E	1.140	1.00
346_R	349_D	1.138	1.00
199_R	205_W	1.121	1.00
23_D	49_S	1.120	1.00
190_V	269_A	1.117	1.00
165_A	243_L	1.113	1.00
199_R	240_I	1.107	1.00
70_I	102_I	1.104	1.00
193_A	272_L	1.099	1.00
255_I	277_P	1.095	1.00
228_E	232_A	1.094	1.00
169_V	183_I	1.091	1.00
323_E	353_E	1.087	1.00
123_H	271_S	1.085	1.00
17_D	20_K	1.080	1.00
286_F	289_A	1.073	1.00
199_R	244_A	1.072	1.00
18_N	42_N	1.071	1.00
208_L	218_R	1.062	1.00
67_V	71_R	1.056	1.00
338_L	348_Y	1.054	1.00
194_I	220_Y	1.052	1.00
196_I	276_I	1.050	1.00
229_I	233_T	1.043	1.00
314_R	317_E	1.041	1.00
184_V	235_L	1.036	1.00
76_E	310_F	1.036	1.00
197_I	276_I	1.034	1.00
349_D	353_E	1.031	1.00
78_I	102_I	1.027	1.00
148_L	159_I	1.026	1.00
206_A	279_L	1.021	1.00
247_Y	255_I	1.019	1.00
167_I	184_V	1.015	1.00
330_M	354_V	1.014	1.00
238_S	241_D	1.013	1.00
135_F	254_T	1.010	1.00
165_A	246_L	1.007	1.00
167_I	182_Y	1.006	1.00
230_E	233_T	1.001	1.00
241_D	245_E	0.997	1.00
195_G	240_I	0.995	1.00
323_E	349_D	0.995	1.00
161_D	164_K	0.994	1.00
319_G	322_I	0.994	1.00
196_I	200_L	0.987	1.00
194_I	224_F	0.986	1.00
69_E	356_L	0.981	1.00
66_I	69_E	0.976	1.00
76_E	105_A	0.973	1.00
52_I	61_E	0.971	1.00
226_D	241_D	0.969	1.00
51_Y	365_C	0.969	1.00
11_D	154_H	0.969	1.00
217_L	279_L	0.966	1.00
136_L	158_L	0.965	1.00
253_L	282_M	0.964	1.00
70_I	331_F	0.958	1.00
215_E	218_R	0.956	1.00
184_V	192_L	0.952	1.00
141_F	159_I	0.952	1.00
48_D	56_L	0.950	1.00
345_D	349_D	0.949	1.00
55_K	61_E	0.945	1.00
207_D	210_L	0.944	1.00
168_D	172_S	0.942	1.00
185_K	234_G	0.940	1.00
220_Y	223_S	0.936	1.00
210_L	214_P	0.932	1.00
192_L	196_I	0.931	1.00
242_E	245_E	0.927	1.00
136_L	161_D	0.927	1.00
124_Y	274_S	0.925	1.00
238_S	242_E	0.925	1.00
171_I	183_I	0.924	1.00
226_D	229_I	0.924	1.00
122_L	271_S	0.923	1.00
171_I	181_Y	0.923	1.00
167_I	243_L	0.922	1.00
258_F	262_R	0.921	1.00
239_K	246_L	0.920	1.00
149_V	174_T	0.914	1.00
165_A	180_K	0.914	1.00
320_K	324_E	0.912	1.00
332_V	368_L	0.909	1.00
140_S	253_L	0.908	1.00
260_L	269_A	0.906	1.00
50_V	58_N	0.898	1.00
317_E	321_E	0.895	1.00
15_F	31_T	0.893	1.00
175_A	181_Y	0.886	1.00
200_L	276_I	0.885	1.00
65_L	68_K	0.878	1.00
225_N	229_I	0.877	1.00
318_V	322_I	0.875	1.00
194_I	221_V	0.874	1.00
283_K	286_F	0.872	1.00
193_A	276_I	0.871	1.00
22_I	39_L	0.866	1.00
235_L	240_I	0.863	1.00
226_D	230_E	0.860	1.00
87_Q	333_W	0.855	1.00
235_L	239_K	0.854	1.00
304_T	307_I	0.853	1.00
91_T	94_Y	0.853	1.00
79_L	330_M	0.852	1.00
49_S	58_N	0.850	1.00
252_P	285_G	0.849	1.00
57_S	62_A	0.848	1.00
199_R	237_S	0.844	1.00
73_R	355_R	0.843	1.00
190_V	272_L	0.842	1.00
48_D	51_Y	0.839	1.00
119_A	274_S	0.838	1.00
63_L	67_V	0.837	1.00
69_E	329_F	0.836	1.00
17_D	22_I	0.836	1.00
198_K	225_N	0.836	1.00
203_R	248_Y	0.833	1.00
143_I	174_T	0.833	1.00
88_G	91_T	0.826	0.99
215_E	219_N	0.824	0.99
195_G	199_R	0.822	0.99
230_E	238_S	0.819	0.99
246_L	250_R	0.818	0.99
220_Y	224_F	0.818	0.99
268_D	271_S	0.816	0.99
311_K	314_R	0.813	0.99
150_F	173_E	0.812	0.99
54_G	57_S	0.811	0.99
201_F	279_L	0.806	0.99
83_Y	107_T	0.805	0.99
76_E	104_A	0.802	0.99
322_I	330_M	0.801	0.99
184_V	233_T	0.801	0.99
244_A	248_Y	0.796	0.99
202_E	226_D	0.796	0.99
19_H	22_I	0.793	0.99
313_C	321_E	0.790	0.99
252_P	283_K	0.789	0.99
182_Y	192_L	0.787	0.99
157_N	161_D	0.785	0.99
338_L	351_V	0.784	0.99
198_K	221_V	0.783	0.99
190_V	265_N	0.779	0.99
51_Y	54_G	0.778	0.99
226_D	237_S	0.778	0.99
140_S	247_Y	0.775	0.99
217_L	220_Y	0.773	0.99
43_S	364_Y	0.769	0.99
328_T	354_V	0.769	0.99
66_I	331_F	0.766	0.99
195_G	226_D	0.763	0.99
245_E	248_Y	0.762	0.99
243_L	246_L	0.761	0.99
150_F	170_R	0.761	0.99
195_G	244_A	0.759	0.99
143_I	148_L	0.758	0.99
237_S	240_I	0.756	0.99
30_F	157_N	0.756	0.99
131_L	134_D	0.755	0.99
18_N	21_P	0.754	0.99
194_I	272_L	0.754	0.99
137_K	252_P	0.752	0.99
197_I	275_L	0.751	0.99
119_A	123_H	0.749	0.99
332_V	360_V	0.749	0.99
142_V	255_I	0.749	0.99
147_E	150_F	0.746	0.99
192_L	235_L	0.742	0.99
171_I	175_A	0.741	0.99
28_N	161_D	0.739	0.99
227_E	230_E	0.738	0.99
68_K	71_R	0.735	0.99
97_R	100_N	0.735	0.99
138_Y	253_L	0.734	0.99
32_C	35_G	0.731	0.99
256_I	287_F	0.731	0.99
218_R	221_V	0.727	0.99
33_S	37_A	0.722	0.99
244_A	276_I	0.721	0.99
60_D	64_D	0.719	0.99
331_F	359_V	0.718	0.99
188_S	191_Y	0.716	0.99
65_L	329_F	0.715	0.99
62_A	66_I	0.714	0.99
16_D	20_K	0.713	0.99
123_H	274_S	0.713	0.99
198_K	229_I	0.712	0.99
19_H	39_L	0.708	0.98
120_I	274_S	0.706	0.98
260_L	270_I	0.705	0.98
191_Y	229_I	0.705	0.98
195_G	231_E	0.704	0.98
247_Y	277_P	0.703	0.98
332_V	337_P	0.703	0.98
277_P	287_F	0.701	0.98
63_L	66_I	0.698	0.98
247_Y	276_I	0.694	0.98
5_C	13_C	0.694	0.98
75_K	103_G	0.694	0.98
319_G	347_I	0.691	0.98
130_A	134_D	0.690	0.98
81_V	335_S	0.690	0.98
47_V	365_C	0.689	0.98
230_E	235_L	0.688	0.98
16_D	22_I	0.687	0.98
252_P	282_M	0.686	0.98
144_W	273_I	0.684	0.98
146_S	290_N	0.682	0.98
195_G	228_E	0.679	0.98
362_D	366_Q	0.678	0.98
322_I	350_A	0.677	0.98
191_Y	228_E	0.676	0.98
113_S	117_H	0.674	0.98
345_D	348_Y	0.671	0.98
64_D	67_V	0.669	0.98
37_A	40_K	0.668	0.98
315_Y	341_L	0.667	0.98
138_Y	252_P	0.663	0.98
322_I	353_E	0.660	0.98
299_K	302_P	0.659	0.98
321_E	324_E	0.658	0.98
123_H	275_L	0.658	0.98
202_E	218_R	0.657	0.97
39_L	42_N	0.654	0.97
130_A	254_T	0.653	0.97
286_F	292_Q	0.650	0.97
306_Q	309_S	0.648	0.97
160_R	177_R	0.648	0.97
211_L	279_L	0.648	0.97
191_Y	195_G	0.648	0.97
168_D	181_Y	0.647	0.97
344_S	348_Y	0.644	0.97
255_I	273_I	0.644	0.97
289_A	292_Q	0.644	0.97
146_S	257_G	0.643	0.97
80_H	95_P	0.643	0.97
195_G	225_N	0.642	0.97
206_A	218_R	0.639	0.97
137_K	284_K	0.634	0.97
166_T	174_T	0.634	0.97
110_S	114_S	0.630	0.97
24_T	28_N	0.629	0.97
168_D	175_A	0.627	0.97
201_F	211_L	0.626	0.97
330_M	358_L	0.625	0.97
329_F	354_V	0.625	0.97
195_G	230_E	0.625	0.97
116_G	256_I	0.623	0.97
139_E	253_L	0.621	0.96
190_V	193_A	0.620	0.96
74_K	328_T	0.617	0.96
168_D	174_T	0.616	0.96
18_N	22_I	0.615	0.96
10_Y	13_C	0.615	0.96
129_G	288_Y	0.613	0.96
148_L	174_T	0.610	0.96
49_S	57_S	0.610	0.96
360_V	366_Q	0.607	0.96
70_I	356_L	0.607	0.96
116_G	270_I	0.606	0.96
195_G	198_K	0.606	0.96
197_I	272_L	0.606	0.96
29_G	53_E	0.605	0.96
326_K	353_E	0.605	0.96
133_E	137_K	0.604	0.96
297_D	300_Y	0.604	0.96
143_I	166_T	0.604	0.96
118_E	267_G	0.602	0.96
52_I	66_I	0.599	0.96
183_I	239_K	0.597	0.96
148_L	290_N	0.596	0.96
113_S	116_G	0.596	0.96
200_L	204_N	0.595	0.96
320_K	323_E	0.594	0.95
36_I	40_K	0.592	0.95
265_N	269_A	0.586	0.95
200_L	205_W	0.584	0.95
198_K	228_E	0.582	0.95
147_E	168_D	0.582	0.95
61_E	64_D	0.582	0.95
124_Y	287_F	0.582	0.95
13_C	32_C	0.581	0.95
198_K	223_S	0.580	0.95
336_N	368_L	0.580	0.95
300_Y	303_R	0.580	0.95
200_L	279_L	0.580	0.95
119_A	267_G	0.580	0.95
99_W	107_T	0.579	0.95
236_D	241_D	0.579	0.95
330_M	351_V	0.579	0.95
240_I	244_A	0.578	0.95
132_P	135_F	0.578	0.95
335_S	341_L	0.577	0.95
298_F	301_L	0.576	0.95
183_I	186_P	0.575	0.95
227_E	231_E	0.574	0.95
72_K	328_T	0.572	0.94
256_I	270_I	0.572	0.94
84_D	87_Q	0.572	0.94
318_V	330_M	0.566	0.94
138_Y	254_T	0.566	0.94
319_G	341_L	0.565	0.94
199_R	248_Y	0.564	0.94
152_F	155_G	0.563	0.94
247_Y	282_M	0.563	0.94
96_A	100_N	0.562	0.94
291_S	294_L	0.562	0.94
305_S	308_L	0.562	0.94
31_T	36_I	0.560	0.94
322_I	327_I	0.559	0.94
70_I	98_L	0.556	0.94
196_I	245_E	0.556	0.94
5_C	35_G	0.556	0.94
277_P	282_M	0.555	0.94
360_V	368_L	0.555	0.94
120_I	124_Y	0.553	0.93
308_L	314_R	0.552	0.93
55_K	65_L	0.552	0.93
111_I	341_L	0.550	0.93
99_W	102_I	0.549	0.93
288_Y	291_S	0.549	0.93
195_G	237_S	0.548	0.93
15_F	38_D	0.548	0.93
70_I	78_I	0.547	0.93
238_S	245_E	0.547	0.93
47_V	54_G	0.547	0.93
263_S	269_A	0.546	0.93
358_L	368_L	0.545	0.93
274_S	287_F	0.544	0.93
212_D	215_E	0.544	0.93
142_V	276_I	0.544	0.93
277_P	280_I	0.543	0.93
144_W	276_I	0.542	0.93
351_V	358_L	0.542	0.93
158_L	161_D	0.541	0.93
266_G	270_I	0.539	0.93
330_M	337_P	0.539	0.93
115_E	261_G	0.539	0.93
313_C	327_I	0.538	0.92
158_L	162_K	0.538	0.92
151_S	257_G	0.535	0.92
72_K	355_R	0.534	0.92
275_L	278_A	0.534	0.92
200_L	244_A	0.533	0.92
5_C	32_C	0.532	0.92
295_G	298_F	0.531	0.92
269_A	273_I	0.531	0.92
15_F	22_I	0.530	0.92
188_S	229_I	0.530	0.92
4_A	12_T	0.530	0.92
304_T	309_S	0.529	0.92
113_S	291_S	0.529	0.92
147_E	259_A	0.529	0.92
313_C	326_K	0.528	0.92
225_N	231_E	0.528	0.92
203_R	226_D	0.527	0.92
211_L	214_P	0.526	0.92
258_F	261_G	0.525	0.92
77_E	326_K	0.524	0.92
157_N	162_K	0.523	0.91
239_K	243_L	0.523	0.91
289_A	294_L	0.523	0.91
145_G	169_V	0.522	0.91
134_D	254_T	0.522	0.91
54_G	365_C	0.521	0.91
40_K	44_L	0.521	0.91
266_G	269_A	0.520	0.91
337_P	368_L	0.520	0.91
81_V	315_Y	0.519	0.91
347_I	351_V	0.519	0.91
271_S	275_L	0.518	0.91
160_R	164_K	0.517	0.91
194_I	217_L	0.517	0.91
172_S	175_A	0.516	0.91
77_E	329_F	0.516	0.91
7_R	10_Y	0.515	0.91
113_S	258_F	0.515	0.91
93_Y	97_R	0.515	0.91
34_R	352_K	0.513	0.91
140_S	180_K	0.513	0.91
229_I	232_A	0.511	0.90
246_L	249_Y	0.510	0.90
198_K	224_F	0.510	0.90
333_W	361_H	0.510	0.90
147_E	173_E	0.508	0.90
140_S	252_P	0.506	0.90
332_V	351_V	0.504	0.90
114_S	118_E	0.503	0.90
23_D	28_N	0.502	0.90
13_C	35_G	0.502	0.90
25_F	29_G	0.501	0.90
217_L	275_L	0.501	0.90
169_V	184_V	0.501	0.90
57_S	63_L	0.500	0.90
321_E	327_I	0.500	0.90
255_I	276_I	0.500	0.90
296_I	300_Y	0.500	0.90

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1h0hA	1	0.9892	100	0.2	Contact Map
1kqfA	3	0.9892	100	0.202	Contact Map
2iv2X	1	0.9892	100	0.22	Contact Map
3ml1A	1	0.9919	100	0.223	Contact Map
2napA	1	0.9919	100	0.225	Contact Map
2e7zA	1	0.9811	100	0.237	Contact Map
2ivfA	1	0.9919	100	0.246	Contact Map
2vpzA	1	0.9784	100	0.256	Contact Map
1q16A	2	0.9892	100	0.265	Contact Map
1ti6A	3	0.9946	100	0.282	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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