Q97U31 - SULSO - GREMLIN Contacts

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Q97U31 - Uncharacterized protein

UniProt:	Q97U31	Sequences: MVWFLILQLIVLLILVAFSATLMAKGTRELERSFGKGIAGGLILGFINALPETIIVIQAVLNGFYDIALGSALGGNILLLTLGIGLISIFYYLKYKSNIITLDSDINIEYSSFLLAVIILGIAIVYGKLNYYIGLFLLSPYIYYIYRRYKNYRGILKNEKNKGNAIKGLAYVMIGGLPLIFISKYLISTISSIASVFNVQPLILAILITPIAAELEENLTAIKLISDSPSSATTALMNFIGSKLENMTLLLSIIGISQTVSLRPSLLYLLLILATSLLALGIIKDRNIKVKEGLSLFGIYAILIAILLRFSA	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	312 (299)
Sequences:	2484 (1504)
Seq/√Len:	87.0

ECOLI - CHAA YRBG
BACSU - CHAA
HALSA - Q9HN44 Q9HS69
SULSO - Q97U31 Q97YX8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
255_G	258_Q	7.468	1.00
143_Y	294_L	4.542	1.00
255_G	260_V	3.842	1.00
166_I	170_A	3.668	1.00
120_L	123_A	3.256	1.00
135_L	139_S	2.927	1.00
91_Y	98_N	2.839	1.00
258_Q	261_S	2.816	1.00
258_Q	264_P	2.731	1.00
6_I	10_I	2.657	1.00
194_A	204_L	2.564	1.00
285_D	292_E	2.533	1.00
132_Y	305_A	2.505	1.00
63_G	260_V	2.491	1.00
255_G	261_S	2.389	1.00
152_Y	156_L	2.376	1.00
16_V	20_A	2.371	1.00
257_S	260_V	2.312	1.00
97_S	287_N	2.305	1.00
255_G	259_T	2.243	1.00
258_Q	262_L	2.177	1.00
123_A	126_Y	2.147	1.00
31_E	40_G	2.147	1.00
136_F	298_G	2.132	1.00
50_L	54_I	2.132	1.00
7_L	11_V	2.115	1.00
209_T	213_A	2.096	1.00
217_E	242_S	2.096	1.00
20_A	23_M	2.092	1.00
95_Y	228_S	2.091	1.00
170_A	174_I	2.060	1.00
18_F	22_L	2.020	1.00
49_A	214_E	1.996	1.00
188_S	191_S	1.914	1.00
158_N	161_N	1.913	1.00
157_K	160_K	1.873	1.00
93_L	96_K	1.849	1.00
246_N	250_L	1.840	1.00
34_F	38_I	1.823	1.00
52_E	217_E	1.822	1.00
140_P	298_G	1.815	1.00
248_T	252_S	1.799	1.00
224_L	234_T	1.776	1.00
110_Y	118_I	1.755	1.00
220_T	224_L	1.751	1.00
31_E	36_K	1.700	1.00
261_S	264_P	1.688	1.00
255_G	262_L	1.672	1.00
239_F	243_K	1.643	1.00
256_I	259_T	1.614	1.00
119_I	123_A	1.602	1.00
41_G	45_G	1.599	1.00
30_L	165_A	1.571	1.00
257_S	261_S	1.565	1.00
81_T	275_T	1.517	1.00
75_G	214_E	1.508	1.00
95_Y	98_N	1.506	1.00
122_I	128_K	1.506	1.00
121_G	125_V	1.504	1.00
260_V	264_P	1.478	0.99
146_Y	150_K	1.478	0.99
293_G	296_L	1.461	0.99
30_L	34_F	1.452	0.99
152_Y	155_I	1.448	0.99
221_A	239_F	1.446	0.99
79_L	242_S	1.431	0.99
46_F	245_E	1.427	0.99
86_L	297_F	1.416	0.99
214_E	218_N	1.415	0.99
55_I	71_S	1.413	0.99
180_I	184_K	1.396	0.99
256_I	260_V	1.376	0.99
157_K	162_K	1.364	0.99
89_I	297_F	1.362	0.99
64_F	201_P	1.361	0.99
167_K	170_A	1.360	0.99
75_G	79_L	1.359	0.99
12_L	185_Y	1.354	0.99
140_P	295_S	1.340	0.99
53_T	79_L	1.340	0.99
260_V	263_R	1.318	0.99
89_I	143_Y	1.311	0.99
43_I	54_I	1.310	0.99
18_F	21_T	1.300	0.99
214_E	246_N	1.294	0.99
259_T	264_P	1.293	0.99
48_N	214_E	1.291	0.99
24_A	186_L	1.280	0.98
44_L	47_I	1.269	0.98
165_A	169_L	1.258	0.98
27_T	44_L	1.253	0.98
79_L	214_E	1.251	0.98
102_L	105_D	1.248	0.98
120_L	299_I	1.229	0.98
98_N	228_S	1.221	0.98
121_G	138_L	1.218	0.98
163_G	167_K	1.213	0.98
189_T	192_S	1.213	0.98
206_I	307_L	1.211	0.98
243_K	246_N	1.210	0.98
66_D	69_L	1.209	0.98
78_L	214_E	1.202	0.98
94_K	226_S	1.200	0.98
16_V	21_T	1.198	0.98
64_F	191_S	1.193	0.98
147_R	152_Y	1.189	0.97
142_I	146_Y	1.181	0.97
123_A	127_G	1.181	0.97
92_Y	98_N	1.177	0.97
171_Y	174_I	1.166	0.97
255_G	264_P	1.149	0.97
124_I	269_L	1.148	0.97
108_I	121_G	1.147	0.97
83_G	214_E	1.141	0.97
120_L	127_G	1.127	0.97
143_Y	290_V	1.122	0.96
74_G	81_T	1.120	0.96
13_L	20_A	1.115	0.96
267_L	306_I	1.114	0.96
10_I	14_I	1.110	0.96
57_I	61_L	1.106	0.96
41_G	183_S	1.102	0.96
256_I	261_S	1.099	0.96
122_I	306_I	1.094	0.96
88_S	292_E	1.089	0.96
136_F	140_P	1.085	0.96
268_Y	271_L	1.082	0.96
72_A	209_T	1.077	0.96
24_A	218_N	1.077	0.96
122_I	301_A	1.075	0.95
121_G	272_I	1.069	0.95
131_Y	135_L	1.065	0.95
68_A	72_A	1.064	0.95
193_I	197_F	1.061	0.95
116_A	247_M	1.058	0.95
147_R	290_V	1.042	0.95
71_S	187_I	1.042	0.95
195_S	201_P	1.041	0.95
177_L	181_F	1.041	0.95
207_L	304_I	1.039	0.95
207_L	210_P	1.038	0.95
89_I	93_L	1.029	0.94
91_Y	228_S	1.028	0.94
184_K	188_S	1.026	0.94
108_I	112_S	1.024	0.94
272_I	275_T	1.013	0.94
149_Y	152_Y	1.009	0.94
72_A	251_L	0.997	0.93
147_R	151_N	0.994	0.93
85_G	91_Y	0.988	0.93
113_F	119_I	0.986	0.93
259_T	263_R	0.985	0.93
245_E	252_S	0.980	0.93
304_I	308_L	0.979	0.93
101_T	104_S	0.979	0.93
162_K	200_Q	0.977	0.93
164_N	167_K	0.975	0.92
211_I	215_L	0.974	0.92
121_G	128_K	0.971	0.92
45_G	52_E	0.971	0.92
24_A	214_E	0.971	0.92
23_M	47_I	0.967	0.92
16_V	23_M	0.967	0.92
209_T	244_L	0.966	0.92
127_G	199_V	0.965	0.92
26_G	47_I	0.963	0.92
99_I	102_L	0.958	0.92
76_N	247_M	0.956	0.92
142_I	145_I	0.956	0.92
19_S	178_P	0.954	0.92
32_R	161_N	0.946	0.91
148_R	237_M	0.944	0.91
209_T	212_A	0.942	0.91
12_L	182_I	0.940	0.91
28_R	31_E	0.932	0.91
72_A	171_Y	0.931	0.91
20_A	43_I	0.929	0.91
90_F	226_S	0.927	0.90
108_I	114_L	0.925	0.90
242_S	246_N	0.920	0.90
242_S	250_L	0.917	0.90
62_N	135_L	0.914	0.90
67_I	71_S	0.912	0.90
121_G	126_Y	0.912	0.90
189_T	196_V	0.911	0.90
266_L	273_L	0.906	0.89
84_I	279_A	0.906	0.89
135_L	298_G	0.903	0.89
32_R	159_E	0.902	0.89
158_N	162_K	0.896	0.89
115_L	122_I	0.891	0.89
86_L	90_F	0.882	0.88
118_I	299_I	0.881	0.88
128_K	158_N	0.880	0.88
18_F	23_M	0.878	0.88
253_I	261_S	0.852	0.86
284_K	299_I	0.850	0.86
142_I	161_N	0.848	0.86
151_N	156_L	0.846	0.86
220_T	238_N	0.845	0.86
34_F	43_I	0.841	0.86
109_E	112_S	0.840	0.86
76_N	246_N	0.837	0.85
72_A	203_I	0.833	0.85
13_L	21_T	0.831	0.85
117_V	123_A	0.830	0.85
33_S	183_S	0.828	0.85
144_Y	148_R	0.826	0.85
154_G	173_M	0.824	0.84
44_L	48_N	0.821	0.84
100_I	288_I	0.818	0.84
141_Y	144_Y	0.814	0.84
26_G	172_V	0.808	0.83
48_N	213_A	0.807	0.83
241_G	247_M	0.806	0.83
134_G	137_L	0.806	0.83
7_L	15_L	0.799	0.83
33_S	165_A	0.798	0.82
49_A	242_S	0.797	0.82
214_E	245_E	0.796	0.82
174_I	178_P	0.796	0.82
255_G	263_R	0.794	0.82
98_N	288_I	0.793	0.82
302_I	306_I	0.792	0.82
162_K	165_A	0.792	0.82
182_I	244_L	0.790	0.82
242_S	245_E	0.786	0.81
143_Y	147_R	0.782	0.81
70_G	205_A	0.779	0.81
85_G	92_Y	0.779	0.81
76_N	243_K	0.778	0.81
87_I	91_Y	0.774	0.80
97_S	103_D	0.774	0.80
264_P	305_A	0.772	0.80
267_L	279_A	0.771	0.80
127_G	174_I	0.770	0.80
179_L	194_A	0.769	0.80
143_Y	153_R	0.768	0.80
301_A	305_A	0.765	0.80
79_L	243_K	0.765	0.80
73_L	206_I	0.764	0.80
109_E	114_L	0.761	0.79
111_S	126_Y	0.759	0.79
211_I	218_N	0.759	0.79
179_L	216_E	0.758	0.79
107_N	125_V	0.757	0.79
90_F	222_I	0.755	0.79
58_Q	183_S	0.753	0.79
14_I	140_P	0.750	0.78
119_I	126_Y	0.750	0.78
20_A	28_R	0.750	0.78
74_G	300_Y	0.746	0.78
199_V	271_L	0.745	0.78
22_L	26_G	0.743	0.78
47_I	50_L	0.741	0.78
289_K	292_E	0.739	0.77
65_Y	262_L	0.737	0.77
176_G	180_I	0.734	0.77
108_I	111_S	0.734	0.77
163_G	177_L	0.733	0.77
26_G	175_G	0.731	0.77
34_F	244_L	0.729	0.76
50_L	179_L	0.727	0.76
183_S	249_L	0.723	0.76
10_I	169_L	0.722	0.76
297_F	301_A	0.721	0.76
88_S	92_Y	0.721	0.76
29_E	87_I	0.718	0.75
259_T	262_L	0.714	0.75
131_Y	236_L	0.714	0.75
155_I	290_V	0.713	0.75
87_I	235_A	0.713	0.75
280_L	284_K	0.712	0.75
58_Q	187_I	0.712	0.75
223_K	226_S	0.709	0.74
276_S	280_L	0.705	0.74
17_A	275_T	0.705	0.74
150_K	153_R	0.703	0.74
153_R	156_L	0.700	0.73
158_N	257_S	0.698	0.73
205_A	210_P	0.698	0.73
208_I	211_I	0.697	0.73
12_L	15_L	0.696	0.73
194_A	223_K	0.696	0.73
92_Y	293_G	0.693	0.73
272_I	279_A	0.689	0.72
89_I	157_K	0.688	0.72
235_A	242_S	0.687	0.72
173_M	257_S	0.686	0.72
43_I	223_K	0.686	0.72
88_S	288_I	0.683	0.72
91_Y	97_S	0.683	0.72
154_G	157_K	0.680	0.71
168_G	172_V	0.673	0.70
77_I	271_L	0.672	0.70
28_R	50_L	0.667	0.70
24_A	221_A	0.666	0.70
34_F	37_G	0.663	0.69
183_S	243_K	0.661	0.69
88_S	131_Y	0.661	0.69
27_T	250_L	0.660	0.69
173_M	302_I	0.659	0.69
31_E	42_L	0.658	0.69
91_Y	95_Y	0.658	0.69
127_G	295_S	0.657	0.69
132_Y	136_F	0.657	0.69
46_F	49_A	0.654	0.68
158_N	170_A	0.651	0.68
64_F	67_I	0.650	0.68
206_I	299_I	0.649	0.68
11_V	99_I	0.649	0.68
125_V	129_L	0.648	0.68
49_A	246_N	0.647	0.67
60_V	293_G	0.646	0.67
186_L	212_A	0.644	0.67
109_E	117_V	0.643	0.67
142_I	174_I	0.643	0.67
67_I	190_I	0.643	0.67
41_G	46_F	0.642	0.67
49_A	79_L	0.639	0.67
50_L	234_T	0.638	0.66
133_I	137_L	0.638	0.66
41_G	48_N	0.635	0.66
71_S	218_N	0.635	0.66
105_D	282_I	0.634	0.66
255_G	295_S	0.634	0.66
190_I	193_I	0.633	0.66
27_T	49_A	0.631	0.66
245_E	248_T	0.631	0.66
44_L	238_N	0.630	0.65
293_G	297_F	0.627	0.65
146_Y	277_L	0.622	0.64
99_I	139_S	0.620	0.64
216_E	238_N	0.615	0.64
295_S	298_G	0.614	0.63
101_T	118_I	0.613	0.63
211_I	214_E	0.613	0.63
79_L	213_A	0.613	0.63
137_L	141_Y	0.612	0.63
56_V	233_T	0.610	0.63
41_G	44_L	0.608	0.63
20_A	27_T	0.608	0.63
155_I	298_G	0.607	0.63
114_L	277_L	0.607	0.63
75_G	242_S	0.602	0.62
147_R	153_R	0.602	0.62
194_A	205_A	0.596	0.61
60_V	63_G	0.596	0.61
131_Y	256_I	0.594	0.61
99_I	161_N	0.593	0.61
261_S	266_L	0.593	0.61
186_L	218_N	0.592	0.61
78_L	300_Y	0.591	0.61
205_A	229_P	0.588	0.60
45_G	56_V	0.588	0.60
147_R	156_L	0.588	0.60
156_L	287_N	0.587	0.60
17_A	141_Y	0.586	0.60
78_L	217_E	0.584	0.60
49_A	53_T	0.583	0.59
87_I	190_I	0.582	0.59
281_G	284_K	0.582	0.59
233_T	237_M	0.580	0.59
20_A	225_I	0.580	0.59
100_I	105_D	0.580	0.59
135_L	192_S	0.579	0.59
214_E	243_K	0.579	0.59
75_G	83_G	0.579	0.59
8_Q	11_V	0.574	0.58
49_A	83_G	0.572	0.58
83_G	133_I	0.571	0.58
48_N	242_S	0.569	0.58
132_Y	162_K	0.569	0.58
71_S	213_A	0.568	0.58
294_L	306_I	0.567	0.57
305_A	308_L	0.567	0.57
120_L	128_K	0.566	0.57
115_L	215_L	0.564	0.57
160_K	287_N	0.563	0.57
21_T	209_T	0.563	0.57
20_A	137_L	0.562	0.57
125_V	139_S	0.561	0.57
114_L	281_G	0.561	0.57
53_T	246_N	0.561	0.57
273_L	277_L	0.560	0.56
51_P	183_S	0.559	0.56
68_A	254_I	0.558	0.56
223_K	227_D	0.558	0.56
16_V	49_A	0.556	0.56
40_G	141_Y	0.556	0.56
297_F	308_L	0.555	0.56
154_G	159_E	0.555	0.56
114_L	178_P	0.554	0.56
16_V	26_G	0.553	0.56
157_K	161_N	0.552	0.55
212_A	216_E	0.552	0.55
86_L	174_I	0.552	0.55
139_S	294_L	0.552	0.55
203_I	292_E	0.550	0.55
224_L	238_N	0.548	0.55
111_S	117_V	0.547	0.55
186_L	201_P	0.547	0.55
139_S	161_N	0.546	0.55
34_F	305_A	0.545	0.54
80_L	186_L	0.544	0.54
70_G	73_L	0.543	0.54
246_N	252_S	0.541	0.54
92_Y	97_S	0.539	0.54
20_A	217_E	0.539	0.54
146_Y	236_L	0.538	0.54
29_E	32_R	0.536	0.53
75_G	218_N	0.536	0.53
257_S	263_R	0.536	0.53
71_S	184_K	0.534	0.53
73_L	251_L	0.532	0.53
10_I	120_L	0.531	0.53
52_E	242_S	0.530	0.52
118_I	128_K	0.530	0.52
66_D	263_R	0.528	0.52
31_E	52_E	0.525	0.52
190_I	279_A	0.524	0.52
81_T	296_L	0.524	0.52
237_M	245_E	0.523	0.51
84_I	286_R	0.522	0.51
45_G	242_S	0.522	0.51
270_L	287_N	0.521	0.51
40_G	241_G	0.521	0.51
42_L	145_I	0.520	0.51
260_V	266_L	0.519	0.51
6_I	169_L	0.518	0.51
112_S	123_A	0.516	0.51
48_N	246_N	0.513	0.50
31_E	224_L	0.512	0.50
199_V	210_P	0.512	0.50
146_Y	154_G	0.510	0.50
16_V	220_T	0.509	0.50
11_V	198_N	0.507	0.49
245_E	250_L	0.506	0.49
147_R	155_I	0.506	0.49
244_L	251_L	0.505	0.49
40_G	183_S	0.505	0.49
132_Y	284_K	0.504	0.49
204_L	212_A	0.502	0.49
190_I	194_A	0.501	0.49
166_I	177_L	0.501	0.49
110_Y	275_T	0.501	0.49
41_G	56_V	0.500	0.48
252_S	293_G	0.500	0.48

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
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HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4k1cA	4	0.9872	100	0.184	Contact Map
4kjrA	3	0.9295	100	0.24	Contact Map
3v5uA	1	0.9423	100	0.261	Contact Map
4kppA	1	0.984	100	0.304	Contact Map
3l1lA	2	0.484	14	0.956	Contact Map
3j1zP	8	0.6154	12.2	0.957	Contact Map
2kncA	1	0.1603	10.5	0.958	Contact Map
3zuxA	1	0.2917	9.7	0.959	Contact Map
3o7qA	1	0.4679	8.9	0.96	Contact Map
4pgrA	1	0.4391	8.3	0.96	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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