Q97U20 - SULSO - GREMLIN Contacts

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Q97U20 - Uncharacterized protein

UniProt:	Q97U20	Sequences: MNIETLMIKNPPILSKEDRLGSAFKKINEGGIGRIIVANEKIEGLLTTRDLLSTVESYCKDSCSQGDLYHISTTPIIDYMTPNPVTVYNTSDEFTAINIMVTRNFGSLPVVDINDKPVGIVTEREFLLLYKDLDEIFPVKVFMSTKVQTIYKEVRLDQAVKLMLRRGFRRLPVIDDDNKVVGIVTVVNAIKQLAKAVDKLDPDYFYGKVVKDVMVTNLVTIDELASVNRAAAEMIVKRIGSLLILNKDNTIRGIITERDLLIALHHILVMEKFKEKL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	277 (217)
Sequences:	1202 (803)
Seq/√Len:	54.5

HALSA - Q9HRK9
SULSO - Q97U20

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
150_I	173_V	4.468	1.00
87_V	110_V	4.159	1.00
175_D	179_K	3.398	1.00
137_F	141_V	3.103	1.00
236_V	239_I	2.865	1.00
159_A	171_L	2.863	1.00
121_V	142_F	2.690	1.00
214_M	217_N	2.685	1.00
51_L	54_T	2.454	1.00
226_S	229_R	2.422	1.00
210_V	213_V	2.368	1.00
221_I	225_A	2.308	1.00
94_F	136_I	2.263	1.00
219_V	224_L	2.257	1.00
215_V	219_V	2.224	1.00
96_A	100_M	2.207	1.00
198_D	201_D	2.173	1.00
154_V	158_Q	2.137	1.00
98_N	102_T	2.125	1.00
217_N	221_I	2.109	1.00
96_A	121_V	2.099	1.00
216_T	225_A	2.059	1.00
217_N	225_A	2.047	1.00
46_L	58_Y	2.041	1.00
92_D	95_T	2.034	1.00
87_V	91_S	2.000	1.00
91_S	95_T	1.957	1.00
88_Y	91_S	1.906	1.00
94_F	98_N	1.889	0.99
196_A	199_K	1.858	0.99
87_V	139_V	1.778	0.99
230_A	233_E	1.729	0.99
201_D	204_Y	1.702	0.99
204_Y	207_G	1.673	0.99
93_E	137_F	1.667	0.99
111_V	115_D	1.663	0.99
231_A	235_I	1.662	0.99
215_V	221_I	1.635	0.99
110_V	139_V	1.577	0.98
109_P	180_V	1.563	0.98
216_T	219_V	1.562	0.98
178_N	181_V	1.534	0.98
89_N	118_V	1.526	0.98
148_Q	166_R	1.515	0.98
97_I	126_F	1.504	0.97
150_I	154_V	1.471	0.97
151_Y	176_D	1.459	0.97
123_E	127_L	1.430	0.96
195_K	198_D	1.404	0.96
223_E	229_R	1.391	0.96
217_N	222_D	1.369	0.95
252_R	256_T	1.360	0.95
87_V	96_A	1.355	0.95
214_M	225_A	1.349	0.95
227_V	235_I	1.337	0.95
222_D	225_A	1.327	0.95
91_S	110_V	1.315	0.94
80_M	173_V	1.302	0.94
223_E	226_S	1.298	0.94
211_K	216_T	1.273	0.93
228_N	232_A	1.271	0.93
216_T	224_L	1.262	0.93
44_G	47_T	1.250	0.93
207_G	210_V	1.250	0.93
231_A	238_R	1.247	0.93
202_P	207_G	1.245	0.93
169_R	186_V	1.244	0.93
209_V	212_D	1.238	0.92
231_A	236_V	1.237	0.92
242_L	246_N	1.231	0.92
216_T	223_E	1.222	0.92
217_N	223_E	1.204	0.91
253_G	256_T	1.194	0.91
220_T	223_E	1.185	0.91
198_D	202_P	1.176	0.90
215_V	224_L	1.162	0.90
195_K	203_D	1.156	0.89
58_Y	72_S	1.146	0.89
214_M	221_I	1.142	0.89
220_T	224_L	1.125	0.88
195_K	199_K	1.123	0.88
138_P	141_V	1.121	0.88
139_V	146_K	1.112	0.88
121_V	189_A	1.081	0.86
152_K	181_V	1.079	0.86
215_V	223_E	1.064	0.85
196_A	200_L	1.055	0.85
55_V	58_Y	1.055	0.85
210_V	221_I	1.054	0.85
216_T	221_I	1.054	0.85
219_V	222_D	1.053	0.85
197_V	200_L	1.042	0.84
218_L	224_L	1.035	0.84
182_G	257_E	1.032	0.83
245_L	256_T	1.031	0.83
147_V	172_P	1.028	0.83
203_D	207_G	1.027	0.83
150_I	172_P	1.017	0.82
113_I	179_K	1.017	0.82
49_R	55_V	1.016	0.82
47_T	55_V	1.013	0.82
215_V	225_A	1.012	0.82
97_I	101_V	1.011	0.82
202_P	206_Y	1.010	0.82
151_Y	154_V	1.005	0.82
219_V	225_A	0.997	0.81
227_V	231_A	0.997	0.81
195_K	201_D	0.992	0.81
224_L	236_V	0.990	0.81
229_R	247_K	0.990	0.81
54_T	58_Y	0.987	0.80
41_K	44_G	0.986	0.80
214_M	219_V	0.985	0.80
229_R	236_V	0.980	0.80
246_N	250_T	0.979	0.80
247_K	258_R	0.979	0.80
217_N	224_L	0.979	0.80
221_I	224_L	0.971	0.79
85_V	108_L	0.967	0.79
215_V	220_T	0.958	0.78
199_K	203_D	0.954	0.78
93_E	142_F	0.949	0.78
76_I	173_V	0.948	0.78
235_I	240_G	0.945	0.77
233_E	237_K	0.936	0.77
253_G	257_E	0.932	0.77
42_I	47_T	0.930	0.76
57_S	112_D	0.923	0.76
78_D	192_Q	0.919	0.75
203_D	206_Y	0.917	0.75
152_K	175_D	0.916	0.75
100_M	171_L	0.915	0.75
228_N	233_E	0.910	0.75
116_K	179_K	0.910	0.75
250_T	253_G	0.908	0.75
246_N	252_R	0.902	0.74
127_L	168_F	0.902	0.74
216_T	222_D	0.898	0.74
198_D	203_D	0.894	0.73
99_I	103_R	0.888	0.73
90_T	113_I	0.884	0.73
57_S	126_F	0.875	0.72
52_L	58_Y	0.870	0.71
96_A	126_F	0.870	0.71
90_T	138_P	0.867	0.71
168_F	188_N	0.860	0.71
234_M	238_R	0.858	0.70
210_V	214_M	0.856	0.70
198_D	204_Y	0.853	0.70
93_E	133_L	0.852	0.70
223_E	236_V	0.851	0.70
72_S	162_L	0.849	0.70
215_V	222_D	0.843	0.69
134_D	137_F	0.842	0.69
140_K	145_T	0.839	0.69
248_D	256_T	0.835	0.68
88_Y	111_V	0.833	0.68
248_D	253_G	0.833	0.68
100_M	108_L	0.824	0.67
125_E	163_M	0.822	0.67
85_V	103_R	0.820	0.67
93_E	135_E	0.816	0.66
91_S	139_V	0.815	0.66
220_T	237_K	0.810	0.66
197_V	204_Y	0.806	0.66
216_T	220_T	0.805	0.65
214_M	224_L	0.798	0.65
150_I	189_A	0.794	0.64
200_L	203_D	0.790	0.64
195_K	200_L	0.786	0.64
51_L	57_S	0.785	0.63
41_K	47_T	0.782	0.63
78_D	122_T	0.777	0.63
98_N	136_I	0.777	0.63
40_E	85_V	0.771	0.62
95_T	98_N	0.771	0.62
41_K	45_L	0.770	0.62
100_M	189_A	0.767	0.62
97_I	163_M	0.767	0.62
172_P	180_V	0.764	0.61
158_Q	161_K	0.760	0.61
54_T	57_S	0.760	0.61
210_V	225_A	0.760	0.61
214_M	223_E	0.759	0.61
99_I	104_N	0.758	0.61
144_S	147_V	0.755	0.60
96_A	108_L	0.755	0.60
71_I	108_L	0.753	0.60
249_N	256_T	0.751	0.60
57_S	102_T	0.747	0.60
113_I	118_V	0.746	0.59
245_L	253_G	0.745	0.59
132_D	137_F	0.743	0.59
38_A	41_K	0.741	0.59
197_V	206_Y	0.741	0.59
53_S	57_S	0.737	0.59
207_G	212_D	0.735	0.58
44_G	52_L	0.735	0.58
224_L	229_R	0.734	0.58
220_T	231_A	0.731	0.58
160_V	163_M	0.730	0.58
170_R	240_G	0.730	0.58
186_V	190_I	0.729	0.58
39_N	76_I	0.728	0.58
50_D	55_V	0.726	0.57
95_T	108_L	0.724	0.57
89_N	140_K	0.722	0.57
210_V	215_V	0.722	0.57
38_A	42_I	0.721	0.57
95_T	99_I	0.720	0.57
154_V	173_V	0.718	0.57
71_I	76_I	0.712	0.56
211_K	214_M	0.709	0.56
233_E	238_R	0.707	0.55
91_S	108_L	0.704	0.55
196_A	201_D	0.703	0.55
120_I	159_A	0.703	0.55
246_N	257_E	0.703	0.55
239_I	242_L	0.697	0.54
80_M	86_T	0.695	0.54
104_N	181_V	0.695	0.54
229_R	237_K	0.690	0.54
70_H	74_T	0.690	0.54
214_M	220_T	0.689	0.54
204_Y	213_V	0.677	0.52
103_R	181_V	0.676	0.52
156_L	189_A	0.675	0.52
95_T	178_N	0.674	0.52
246_N	258_R	0.673	0.52
207_G	211_K	0.671	0.52
170_R	184_V	0.671	0.52
98_N	101_V	0.670	0.51
249_N	252_R	0.669	0.51
228_N	231_A	0.668	0.51
43_E	51_L	0.666	0.51
211_K	215_V	0.664	0.51
247_K	250_T	0.661	0.51
121_V	186_V	0.660	0.50
50_D	58_Y	0.660	0.50
42_I	45_L	0.658	0.50
139_V	145_T	0.656	0.50
224_L	231_A	0.654	0.50
199_K	207_G	0.653	0.50
106_G	186_V	0.645	0.49
227_V	232_A	0.642	0.48
208_K	212_D	0.638	0.48
120_I	147_V	0.634	0.48
247_K	252_R	0.632	0.47
88_Y	113_I	0.630	0.47
71_I	181_V	0.628	0.47
247_K	257_E	0.627	0.47
88_Y	133_L	0.623	0.46
142_F	190_I	0.619	0.46
252_R	258_R	0.617	0.46
51_L	56_E	0.614	0.46
201_D	205_F	0.613	0.45
229_R	235_I	0.613	0.45
40_E	160_V	0.613	0.45
153_E	176_D	0.612	0.45
88_Y	192_Q	0.612	0.45
89_N	112_D	0.609	0.45
39_N	173_V	0.605	0.45
253_G	258_R	0.604	0.44
201_D	206_Y	0.603	0.44
76_I	150_I	0.602	0.44
171_L	234_M	0.599	0.44
85_V	105_F	0.598	0.44
98_N	115_D	0.596	0.44
159_A	170_R	0.596	0.44
196_A	213_V	0.592	0.43
117_P	174_I	0.590	0.43
195_K	207_G	0.590	0.43
190_I	193_L	0.589	0.43
118_V	142_F	0.586	0.43
235_I	238_R	0.584	0.42
80_M	186_V	0.584	0.42
82_P	85_V	0.584	0.42
232_A	236_V	0.581	0.42
229_R	232_A	0.580	0.42
220_T	232_A	0.576	0.42
250_T	257_E	0.574	0.41
170_R	244_I	0.571	0.41
171_L	241_S	0.570	0.41
224_L	247_K	0.568	0.41
182_G	258_R	0.567	0.41
109_P	251_I	0.566	0.40
220_T	228_N	0.566	0.40
93_E	124_R	0.565	0.40
166_R	173_V	0.563	0.40
150_I	167_G	0.560	0.40
76_I	184_V	0.559	0.40
126_F	176_D	0.558	0.40
107_S	170_R	0.556	0.39
233_E	236_V	0.556	0.39
237_K	240_G	0.553	0.39
40_E	51_L	0.550	0.39
147_V	156_L	0.550	0.39
57_S	98_N	0.546	0.38
84_P	191_K	0.544	0.38
160_V	194_A	0.543	0.38
249_N	253_G	0.538	0.38
39_N	172_P	0.538	0.38
88_Y	114_N	0.535	0.37
93_E	178_N	0.535	0.37
165_R	177_D	0.533	0.37
87_V	126_F	0.533	0.37
86_T	139_V	0.532	0.37
39_N	44_G	0.528	0.37
47_T	54_T	0.528	0.37
156_L	186_V	0.527	0.37
125_E	144_S	0.526	0.36
52_L	55_V	0.524	0.36
44_G	48_T	0.521	0.36
247_K	253_G	0.519	0.36
92_D	97_I	0.519	0.36
47_T	50_D	0.518	0.36
121_V	127_L	0.517	0.36
105_F	123_E	0.517	0.36
40_E	43_E	0.516	0.35
71_I	152_K	0.509	0.35
106_G	187_V	0.509	0.35
46_L	54_T	0.506	0.34
252_R	257_E	0.504	0.34
81_T	180_V	0.502	0.34
64_S	75_P	0.501	0.34
250_T	258_R	0.500	0.34

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3ddjA	1	0.9856	100	0.552	Contact Map
2yzqA	2	0.7906	100	0.58	Contact Map
3kh5A	1	0.9531	100	0.592	Contact Map
2qrdG	1	0.8014	100	0.594	Contact Map
3t4nC	1	0.7906	100	0.6	Contact Map
2v8qE	1	0.9531	99.9	0.621	Contact Map
3l2bA	2	0.4224	99.9	0.642	Contact Map
1vrdA	3	0.5199	99.9	0.649	Contact Map
4af0A	3	0.5054	99.9	0.656	Contact Map
1jcnA	6	0.769	99.9	0.658	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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