Q97TV5 - SULSO - GREMLIN Contacts

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Q97TV5 - Transposase ISC1048

UniProt:	Q97TV5	Sequences: MQDLVQAYKEEKNARIKERILAVKLHVVDGKSEKEVSKMLNKGYSTIKLWIGKYKKEGLDGLKDKPRSGRPRKVEEEKIKQILEDKPQKYGIQQEYWTMKTLKIALQEQGIEYKKSRLYELVHELGYNLVKPRPTNIQAEKDKWEDFKKIKKLERKALFFLDECRTVISTSIKKVLAKVGSKPVMRVNIGFSSIYVILAINAWTGEVVVSLAKRPNSESVKYFLRYFKRRVGSGRVYMVMDNYSPHKTKGTLEVCRRKGIHPVFTPPYSPELNMAEAVFKSLKNYMSNKIFYTIEDVKNCIKQFFEENKYRFNLNAITYLGLDKIEV	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	327 (284)
Sequences:	2061 (1711)
Seq/√Len:	101.5

SULSO - Q97TV5 Q7LX66 Q97XL9 Q97TW3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
37_S	47_I	3.511	1.00
217_S	248_T	3.310	1.00
99_M	119_Y	2.978	1.00
31_K	35_E	2.955	1.00
143_K	146_D	2.857	1.00
220_V	240_M	2.856	1.00
27_V	36_V	2.670	1.00
25_L	36_V	2.594	1.00
216_N	219_S	2.587	1.00
143_K	147_F	2.548	1.00
164_C	283_K	2.508	1.00
44_Y	48_K	2.492	1.00
68_S	71_P	2.454	1.00
57_E	61_G	2.431	1.00
221_K	251_T	2.419	1.00
36_V	47_I	2.402	1.00
237_Y	263_V	2.354	1.00
114_K	120_E	2.311	1.00
144_W	148_K	2.248	1.00
221_K	225_R	2.247	1.00
34_K	44_Y	2.190	1.00
146_D	149_K	2.186	1.00
27_V	51_I	2.170	1.00
115_K	119_Y	2.144	1.00
240_M	262_P	2.133	1.00
161_L	238_M	2.133	1.00
31_K	39_M	2.118	1.00
33_E	48_K	2.083	1.00
198_L	279_F	2.049	1.00
201_N	204_T	2.047	1.00
114_K	117_R	2.021	1.00
25_L	39_M	2.015	1.00
116_S	120_E	1.999	1.00
35_E	38_K	1.996	1.00
25_L	40_L	1.951	1.00
117_R	121_L	1.945	1.00
166_T	196_V	1.902	1.00
35_E	39_M	1.815	1.00
227_F	230_R	1.808	1.00
34_K	38_K	1.797	1.00
99_M	115_K	1.785	1.00
43_G	46_T	1.767	1.00
79_I	124_E	1.735	1.00
273_N	276_E	1.733	1.00
239_V	265_T	1.731	1.00
150_I	153_L	1.715	1.00
144_W	147_F	1.706	1.00
116_S	119_Y	1.696	1.00
72_R	79_I	1.684	1.00
302_K	306_E	1.656	1.00
20_I	24_K	1.631	1.00
198_L	275_A	1.618	1.00
45_S	48_K	1.611	1.00
304_F	308_N	1.603	1.00
18_E	40_L	1.603	1.00
74_V	78_K	1.584	1.00
221_K	250_G	1.579	1.00
78_K	81_Q	1.570	1.00
87_P	90_Y	1.553	1.00
292_Y	296_D	1.538	1.00
215_P	243_Y	1.532	1.00
79_I	121_L	1.487	1.00
297_V	301_I	1.482	1.00
117_R	120_E	1.481	1.00
241_D	246_H	1.480	1.00
64_D	67_R	1.467	1.00
113_Y	117_R	1.453	1.00
217_S	221_K	1.438	1.00
285_Y	304_F	1.436	1.00
25_L	31_K	1.432	1.00
33_E	44_Y	1.421	1.00
222_Y	226_Y	1.416	1.00
36_V	51_I	1.411	1.00
249_K	253_E	1.409	1.00
83_L	125_L	1.408	1.00
298_K	302_K	1.402	1.00
18_E	21_L	1.394	1.00
37_S	40_L	1.383	1.00
16_I	23_V	1.381	1.00
163_E	246_H	1.376	0.99
240_M	255_C	1.375	0.99
86_K	90_Y	1.372	0.99
52_G	56_K	1.371	0.99
140_E	143_K	1.358	0.99
45_S	49_L	1.357	0.99
62_L	66_P	1.353	0.99
199_A	206_E	1.349	0.99
18_E	42_K	1.338	0.99
160_F	164_C	1.336	0.99
31_K	36_V	1.324	0.99
119_Y	123_H	1.319	0.99
165_R	193_S	1.316	0.99
75_E	78_K	1.308	0.99
165_R	195_Y	1.307	0.99
275_A	279_F	1.299	0.99
280_K	284_N	1.293	0.99
83_L	127_Y	1.286	0.99
33_E	51_I	1.281	0.99
299_N	303_Q	1.270	0.99
36_V	40_L	1.251	0.99
221_K	254_V	1.249	0.99
294_I	298_K	1.248	0.99
199_A	223_F	1.211	0.99
161_L	199_A	1.202	0.99
255_C	262_P	1.200	0.99
164_C	169_S	1.192	0.99
32_S	35_E	1.192	0.99
17_K	20_I	1.191	0.99
103_K	118_L	1.190	0.99
51_I	55_K	1.188	0.99
129_L	174_K	1.186	0.98
74_V	121_L	1.182	0.98
67_R	71_P	1.179	0.98
238_M	260_I	1.171	0.98
100_K	115_K	1.169	0.98
228_K	260_I	1.165	0.98
220_V	224_L	1.165	0.98
49_L	52_G	1.153	0.98
25_L	29_D	1.150	0.98
281_S	285_Y	1.148	0.98
217_S	251_T	1.148	0.98
304_F	307_E	1.147	0.98
82_I	102_L	1.142	0.98
293_T	296_D	1.123	0.98
70_R	73_K	1.107	0.98
52_G	55_K	1.106	0.98
113_Y	118_L	1.101	0.98
296_D	299_N	1.095	0.97
167_V	193_S	1.091	0.97
54_Y	58_G	1.089	0.97
23_V	54_Y	1.089	0.97
160_F	265_T	1.081	0.97
225_R	229_R	1.081	0.97
188_N	191_F	1.080	0.97
138_Q	146_D	1.070	0.97
248_T	251_T	1.065	0.97
83_L	87_P	1.060	0.97
81_Q	85_D	1.054	0.97
303_Q	307_E	1.054	0.97
187_V	190_G	1.051	0.97
229_R	258_K	1.043	0.97
225_R	254_V	1.043	0.97
75_E	79_I	1.037	0.96
22_A	42_K	1.036	0.96
178_K	181_S	1.028	0.96
253_E	257_R	1.028	0.96
102_L	118_L	1.025	0.96
85_D	90_Y	1.025	0.96
217_S	250_G	1.022	0.96
139_A	142_D	1.021	0.96
98_T	101_T	1.016	0.96
21_L	24_K	1.003	0.96
185_M	190_G	1.001	0.96
186_R	190_G	0.990	0.95
86_K	102_L	0.979	0.95
161_L	240_M	0.979	0.95
141_K	145_E	0.979	0.95
82_I	121_L	0.975	0.95
231_V	236_V	0.974	0.95
240_M	264_F	0.972	0.95
160_F	166_T	0.970	0.95
161_L	223_F	0.967	0.95
242_N	247_K	0.966	0.95
160_F	239_V	0.953	0.94
164_C	301_I	0.940	0.94
161_L	220_V	0.933	0.94
165_R	245_P	0.933	0.94
154_E	157_A	0.932	0.94
86_K	122_V	0.931	0.94
27_V	33_E	0.927	0.93
210_S	298_K	0.924	0.93
167_V	171_S	0.923	0.93
80_K	84_E	0.923	0.93
163_E	297_V	0.920	0.93
21_L	28_V	0.919	0.93
214_R	219_S	0.909	0.93
202_A	205_G	0.906	0.93
215_P	244_S	0.903	0.93
121_L	125_L	0.899	0.92
235_R	261_H	0.897	0.92
57_E	65_K	0.895	0.92
194_I	282_L	0.893	0.92
123_H	201_N	0.893	0.92
17_K	21_L	0.892	0.92
155_R	160_F	0.887	0.92
16_I	20_I	0.882	0.92
277_A	280_K	0.879	0.92
276_E	279_F	0.866	0.91
48_K	51_I	0.866	0.91
307_E	310_Y	0.864	0.91
28_V	35_E	0.864	0.91
210_S	226_Y	0.863	0.91
50_W	62_L	0.863	0.91
138_Q	142_D	0.863	0.91
22_A	50_W	0.860	0.91
29_D	39_M	0.860	0.91
175_V	183_P	0.859	0.91
79_I	125_L	0.857	0.90
283_K	287_S	0.853	0.90
159_F	201_N	0.853	0.90
188_N	195_Y	0.850	0.90
180_G	183_P	0.850	0.90
74_V	79_I	0.848	0.90
168_I	297_V	0.848	0.90
99_M	176_L	0.844	0.90
42_K	47_I	0.842	0.90
224_L	254_V	0.839	0.90
78_K	111_I	0.839	0.90
38_K	41_N	0.836	0.89
72_R	124_E	0.836	0.89
253_E	256_R	0.833	0.89
300_C	304_F	0.830	0.89
185_M	188_N	0.828	0.89
43_G	49_L	0.825	0.89
103_K	115_K	0.818	0.88
177_A	183_P	0.807	0.88
185_M	191_F	0.805	0.88
291_F	300_C	0.804	0.87
80_K	87_P	0.804	0.87
291_F	297_V	0.802	0.87
220_V	238_M	0.800	0.87
46_T	50_W	0.793	0.87
39_M	56_K	0.792	0.87
289_K	300_C	0.791	0.87
34_K	48_K	0.791	0.87
64_D	68_S	0.786	0.86
165_R	170_T	0.785	0.86
83_L	86_K	0.784	0.86
33_E	37_S	0.781	0.86
29_D	32_S	0.771	0.85
265_T	268_Y	0.766	0.85
159_F	199_A	0.765	0.85
100_K	288_N	0.765	0.85
33_E	40_L	0.763	0.85
71_P	100_K	0.761	0.84
181_S	185_M	0.754	0.84
227_F	231_V	0.753	0.84
283_K	301_I	0.752	0.84
24_K	29_D	0.751	0.84
54_Y	62_L	0.749	0.83
34_K	306_E	0.748	0.83
80_K	83_L	0.747	0.83
177_A	180_G	0.747	0.83
196_V	301_I	0.745	0.83
170_T	287_S	0.745	0.83
250_G	257_R	0.740	0.83
164_C	215_P	0.738	0.83
132_P	175_V	0.737	0.82
87_P	178_K	0.735	0.82
290_I	310_Y	0.732	0.82
191_F	199_A	0.731	0.82
51_I	54_Y	0.729	0.82
239_V	268_Y	0.727	0.82
22_A	36_V	0.725	0.81
100_K	104_I	0.724	0.81
244_S	247_K	0.723	0.81
21_L	29_D	0.717	0.81
100_K	224_L	0.713	0.80
116_S	123_H	0.706	0.80
216_N	221_K	0.705	0.80
221_K	248_T	0.701	0.79
207_V	230_R	0.700	0.79
254_V	258_K	0.697	0.79
129_L	176_L	0.693	0.78
162_D	246_H	0.693	0.78
160_F	200_I	0.688	0.78
164_C	279_F	0.685	0.78
22_A	39_M	0.682	0.77
282_L	304_F	0.680	0.77
75_E	81_Q	0.679	0.77
181_S	184_V	0.679	0.77
179_V	182_K	0.672	0.76
79_I	82_I	0.669	0.76
254_V	257_R	0.667	0.76
274_M	278_V	0.666	0.76
53_K	65_K	0.664	0.75
45_S	52_G	0.662	0.75
176_L	270_P	0.662	0.75
207_V	227_F	0.659	0.75
306_E	310_Y	0.658	0.75
265_T	270_P	0.657	0.75
23_V	27_V	0.657	0.75
130_V	265_T	0.655	0.74
138_Q	240_M	0.649	0.74
239_V	273_N	0.642	0.73
160_F	274_M	0.642	0.73
255_C	260_I	0.641	0.73
163_E	166_T	0.640	0.73
161_L	197_I	0.639	0.73
226_Y	262_P	0.635	0.72
294_I	297_V	0.633	0.72
278_V	305_F	0.631	0.72
175_V	181_S	0.631	0.72
242_N	276_E	0.624	0.71
224_L	255_C	0.622	0.71
79_I	86_K	0.621	0.71
135_T	271_E	0.617	0.70
85_D	97_W	0.616	0.70
84_E	87_P	0.611	0.69
161_L	265_T	0.611	0.69
77_E	84_E	0.610	0.69
303_Q	306_E	0.609	0.69
16_I	27_V	0.608	0.69
209_V	305_F	0.607	0.69
82_I	125_L	0.605	0.69
165_R	169_S	0.605	0.69
29_D	38_K	0.604	0.68
228_K	258_K	0.604	0.68
244_S	249_K	0.604	0.68
17_K	24_K	0.603	0.68
159_F	300_C	0.602	0.68
300_C	303_Q	0.602	0.68
216_N	245_P	0.602	0.68
19_R	30_G	0.601	0.68
99_M	113_Y	0.600	0.68
145_E	267_P	0.600	0.68
34_K	56_K	0.598	0.68
249_K	255_C	0.597	0.68
23_V	32_S	0.596	0.67
42_K	46_T	0.595	0.67
178_K	183_P	0.594	0.67
136_N	268_Y	0.590	0.67
38_K	48_K	0.589	0.66
198_L	208_V	0.588	0.66
122_V	176_L	0.588	0.66
87_P	127_Y	0.588	0.66
200_I	274_M	0.588	0.66
81_Q	108_E	0.588	0.66
166_T	220_V	0.585	0.66
128_N	179_V	0.584	0.66
47_I	51_I	0.583	0.66
295_E	299_N	0.581	0.65
249_K	252_L	0.579	0.65
215_P	254_V	0.578	0.65
252_L	256_R	0.577	0.65
279_F	286_M	0.577	0.65
243_Y	267_P	0.574	0.65
166_T	304_F	0.574	0.65
220_V	247_K	0.573	0.64
21_L	25_L	0.573	0.64
293_T	309_K	0.569	0.64
73_K	78_K	0.569	0.64
151_K	233_S	0.569	0.64
295_E	298_K	0.568	0.64
79_I	84_E	0.568	0.64
22_A	47_I	0.568	0.64
22_A	255_C	0.567	0.64
291_F	296_D	0.566	0.63
29_D	36_V	0.566	0.63
16_I	31_K	0.565	0.63
69_G	73_K	0.564	0.63
135_T	140_E	0.564	0.63
283_K	302_K	0.563	0.63
160_F	165_R	0.562	0.63
139_A	143_K	0.562	0.63
226_Y	302_K	0.562	0.63
218_E	250_G	0.559	0.62
195_Y	215_P	0.559	0.62
248_T	252_L	0.559	0.62
161_L	279_F	0.558	0.62
16_I	19_R	0.558	0.62
240_M	265_T	0.557	0.62
286_M	301_I	0.555	0.62
195_Y	243_Y	0.554	0.62
303_Q	310_Y	0.554	0.62
242_N	268_Y	0.553	0.62
35_E	56_K	0.553	0.62
139_A	146_D	0.553	0.62
109_Q	112_E	0.553	0.62
215_P	220_V	0.552	0.62
256_R	299_N	0.552	0.62
89_K	101_T	0.551	0.61
287_S	301_I	0.550	0.61
46_T	49_L	0.550	0.61
34_K	145_E	0.547	0.61
184_V	188_N	0.547	0.61
35_E	45_S	0.546	0.61
98_T	259_G	0.544	0.60
166_T	301_I	0.544	0.60
216_N	228_K	0.540	0.60
197_I	220_V	0.538	0.60
77_E	81_Q	0.538	0.60
156_K	202_A	0.537	0.59
291_F	294_I	0.536	0.59
191_F	263_V	0.535	0.59
80_K	88_Q	0.534	0.59
266_P	272_L	0.533	0.59
68_S	81_Q	0.532	0.59
215_P	219_S	0.532	0.59
155_R	158_L	0.531	0.59
290_I	296_D	0.530	0.58
21_L	40_L	0.527	0.58
133_R	182_K	0.527	0.58
139_A	271_E	0.527	0.58
36_V	50_W	0.526	0.58
296_D	307_E	0.526	0.58
29_D	44_Y	0.526	0.58
83_L	251_T	0.525	0.58
131_K	284_N	0.524	0.58
45_S	55_K	0.524	0.58
271_E	279_F	0.523	0.57
289_K	303_Q	0.522	0.57
38_K	70_R	0.522	0.57
108_E	111_I	0.522	0.57
99_M	118_L	0.520	0.57
157_A	261_H	0.518	0.57
128_N	186_R	0.517	0.57
240_M	246_H	0.515	0.56
53_K	61_G	0.514	0.56
75_E	80_K	0.513	0.56
201_N	206_E	0.513	0.56
242_N	246_H	0.511	0.56
142_D	267_P	0.511	0.56
288_N	291_F	0.510	0.56
129_L	132_P	0.510	0.56
53_K	56_K	0.510	0.56
119_Y	122_V	0.509	0.55
104_I	111_I	0.508	0.55
298_K	301_I	0.507	0.55
101_T	288_N	0.505	0.55
177_A	182_K	0.502	0.54
166_T	170_T	0.501	0.54
23_V	33_E	0.501	0.54
280_K	297_V	0.501	0.54
301_I	305_F	0.500	0.54
196_V	205_G	0.500	0.54

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4u7bA	2	0.9235	100	0.387	Contact Map
3f2kA	1	0.5291	100	0.509	Contact Map
4mdaA	1	0.5229	100	0.517	Contact Map
4fcyA	4	0.8746	99.9	0.666	Contact Map
1u78A	1	0.315	99.7	0.748	Contact Map
1pdnC	1	0.3486	99.7	0.765	Contact Map
1k78A	1	0.3578	99.7	0.766	Contact Map
2k27A	1	0.4281	99.6	0.767	Contact Map
1hlvA	1	0.3609	98.6	0.857	Contact Map
4mq3A	1	0.3914	98.4	0.866	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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