Q7LXD9 - SULSO - GREMLIN Contacts

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Q7LXD9 - Transposase ISC1476

UniProt:	Q7LXD9	Sequences: MPSSGQLYGDEERELTPTPAIPEEGVYEVKYSNRRTNVVRLLPNGFQERKLRRLANLSAKLFNEVNFERRQQFFHEGKVNIEETYKKYYEKYKVILGVNAQAVLNKNNEAWSSFFSLLKLKKEGKLPPHMNHVSPPGYWKDREAGKRKQILVIRQDRYKVDAENHKLILKDFDMEIDFIGRLKWYGKQGRLEIIFDETRNAWYAHIPIEVGVEETKAGKKSKHVVKGERKSIQISKPKGNKVASIDLGINVIASVVVNDGTWLLYKGVRTKEDYFYFQKRIAEVQSLADRTRNIGEYDAYLELLREKRRLFKKLTRRLTHLYRNLASHLIETLYELGVSTIYLGYPFNIVQEKGNKFTVNMWSYRKLMESIELKAQEYGMKVFEVVEYNTSKYCAYHDVEVKRNPRGVINCPLGHKLHSDLNGALNILKKALGVIVNAVKKPLSFIVDHNRVAPIKGCNP	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	460 (349)
Sequences:	3532 (2251)
Seq/√Len:	120.5

ECOLI - INSQ
HALSA - Q9HHJ9 Q9HSW9 Q9HM87 Q9HSW7 Q9HHG8 Q9HSZ6 Q9HSD4 Q9HHU6 Q9HSY4 Q9HHX8
SULSO - Q7LXD9 Q7LXH2 Q97Y67 Q97XP1 Q97V47 Q97X39 Q97V25 Q97Y44 Q97YI2 Q97XQ8 Q97YI5 Q97Y48 Q97YW4 Q97WZ3 Q97WY6 Q97XQ0 Q97Z61 Q97U51 Q97YU1 Q97U68 Q97UQ8 Q97XB6 Q97YW9 Q97XA4 Q97V53 Q7LXH6 Q7LXH9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
320_H	324_N	5.401	1.00
395_A	422_N	4.112	1.00
375_A	382_V	3.543	1.00
342_Y	431_A	3.281	1.00
252_I	367_L	3.198	1.00
368_M	384_E	2.899	1.00
35_R	377_E	2.854	1.00
343_L	371_I	2.771	1.00
366_K	370_S	2.637	1.00
327_S	331_E	2.603	1.00
350_V	359_V	2.600	1.00
330_I	375_A	2.482	1.00
189_G	209_E	2.469	1.00
367_L	371_I	2.455	1.00
385_V	427_I	2.432	1.00
191_L	204_A	2.428	1.00
278_Q	356_K	2.398	1.00
366_K	369_E	2.383	1.00
430_K	433_G	2.361	1.00
403_R	406_R	2.330	1.00
392_K	402_K	2.235	1.00
284_V	303_L	2.186	1.00
323_R	373_L	2.164	1.00
372_E	384_E	2.157	1.00
67_F	71_Q	2.153	1.00
390_T	427_I	2.115	1.00
44_N	47_Q	2.112	1.00
323_R	370_S	2.091	1.00
397_H	413_L	2.047	1.00
341_I	375_A	2.040	1.00
397_H	411_C	2.029	1.00
303_L	307_K	2.017	1.00
389_N	392_K	2.012	1.00
343_L	382_V	1.996	1.00
82_E	86_K	1.996	1.00
326_A	370_S	1.981	1.00
70_R	135_P	1.966	1.00
298_D	301_L	1.962	1.00
65_V	104_L	1.936	1.00
394_C	413_L	1.926	1.00
98_V	154_R	1.925	1.00
410_N	416_K	1.900	1.00
394_C	411_C	1.849	1.00
323_R	326_A	1.847	1.00
407_G	419_S	1.840	1.00
277_F	317_R	1.833	1.00
396_Y	415_H	1.825	1.00
339_S	380_M	1.819	1.00
323_R	374_K	1.807	1.00
66_N	107_N	1.801	1.00
252_I	321_L	1.796	1.00
394_C	397_H	1.765	1.00
83_E	86_K	1.757	1.00
409_I	417_L	1.740	1.00
53_R	94_V	1.736	1.00
52_R	195_F	1.729	1.00
301_L	304_L	1.728	1.00
245_I	253_A	1.707	1.00
319_T	366_K	1.697	1.00
82_E	85_Y	1.677	1.00
425_L	429_K	1.662	1.00
40_R	201_A	1.651	1.00
392_K	401_V	1.644	1.00
60_K	91_K	1.633	1.00
322_Y	366_K	1.630	1.00
52_R	202_W	1.625	1.00
201_A	312_K	1.596	1.00
36_T	205_H	1.562	1.00
251_V	254_S	1.526	1.00
402_K	406_R	1.525	1.00
372_E	376_Q	1.515	1.00
332_T	338_V	1.506	1.00
167_L	204_A	1.503	1.00
352_E	355_N	1.496	1.00
178_F	182_L	1.496	1.00
255_V	259_D	1.489	1.00
326_A	373_L	1.487	1.00
409_I	415_H	1.487	1.00
408_V	416_K	1.476	1.00
180_G	183_K	1.470	1.00
254_S	421_L	1.465	1.00
281_I	311_F	1.451	1.00
287_L	291_T	1.441	1.00
55_A	202_W	1.435	1.00
322_Y	370_S	1.433	1.00
243_A	255_V	1.432	1.00
281_I	307_K	1.427	1.00
288_A	304_L	1.385	1.00
179_I	182_L	1.383	1.00
51_L	202_W	1.360	1.00
239_G	339_S	1.355	1.00
343_L	368_M	1.347	1.00
117_L	133_V	1.339	1.00
343_L	384_E	1.317	1.00
98_V	156_D	1.312	1.00
242_V	428_L	1.312	1.00
199_R	312_K	1.312	1.00
291_T	303_L	1.309	1.00
404_N	407_G	1.307	1.00
174_M	179_I	1.298	1.00
277_F	314_L	1.294	1.00
312_K	316_R	1.293	1.00
49_R	53_R	1.292	1.00
58_S	103_V	1.288	1.00
108_N	112_S	1.284	1.00
65_V	107_N	1.274	1.00
326_A	374_K	1.274	1.00
358_T	364_Y	1.273	1.00
327_S	378_Y	1.269	1.00
344_G	387_E	1.255	0.99
372_E	382_V	1.242	0.99
106_K	152_V	1.238	0.99
48_E	52_R	1.236	0.99
403_R	419_S	1.227	0.99
370_S	373_L	1.217	0.99
62_F	103_V	1.216	0.99
247_L	364_Y	1.213	0.99
256_V	424_A	1.207	0.99
96_L	99_N	1.203	0.99
288_A	303_L	1.199	0.99
395_A	425_L	1.199	0.99
284_V	310_L	1.183	0.99
284_V	307_K	1.181	0.99
345_Y	384_E	1.167	0.99
390_T	430_K	1.162	0.99
295_G	300_Y	1.159	0.99
385_V	430_K	1.153	0.99
194_I	205_H	1.148	0.99
113_S	133_V	1.148	0.99
81_I	86_K	1.142	0.99
174_M	178_F	1.142	0.99
112_S	116_S	1.138	0.99
70_R	74_F	1.133	0.99
45_G	48_E	1.133	0.99
180_G	184_W	1.121	0.99
169_L	193_I	1.116	0.99
326_A	367_L	1.115	0.99
33_N	212_V	1.115	0.99
254_S	264_L	1.113	0.99
263_L	418_H	1.111	0.99
111_W	135_P	1.111	0.99
347_F	387_E	1.105	0.99
174_M	180_G	1.101	0.99
284_V	287_L	1.098	0.99
326_A	371_I	1.095	0.99
178_F	183_K	1.092	0.99
178_F	181_R	1.086	0.98
402_K	419_S	1.083	0.98
328_H	332_T	1.082	0.98
331_E	378_Y	1.078	0.98
330_I	378_Y	1.071	0.98
285_Q	289_D	1.068	0.98
116_S	132_H	1.066	0.98
393_Y	430_K	1.061	0.98
403_R	407_G	1.059	0.98
385_V	431_A	1.055	0.98
109_E	113_S	1.052	0.98
87_K	90_E	1.048	0.98
322_Y	326_A	1.041	0.98
82_E	105_N	1.038	0.98
242_V	432_L	1.037	0.98
37_N	208_I	1.037	0.98
350_V	355_N	1.031	0.98
280_R	283_E	1.024	0.98
315_T	319_T	1.023	0.98
82_E	104_L	1.023	0.98
153_I	169_L	1.019	0.98
421_L	425_L	1.016	0.98
81_I	84_T	1.014	0.98
92_Y	100_A	1.011	0.98
281_I	314_L	1.011	0.98
400_E	409_I	1.011	0.98
245_I	255_V	1.010	0.98
407_G	418_H	1.009	0.98
417_L	421_L	1.009	0.98
288_A	307_K	1.007	0.98
242_V	340_T	1.006	0.97
102_A	105_N	1.000	0.97
114_F	135_P	0.997	0.97
322_Y	367_L	0.991	0.97
86_K	104_L	0.979	0.97
242_V	342_Y	0.974	0.97
247_L	253_A	0.971	0.97
329_L	341_I	0.966	0.97
69_R	108_N	0.965	0.97
43_P	51_L	0.965	0.97
113_S	116_S	0.956	0.97
114_F	136_P	0.954	0.97
285_Q	307_K	0.952	0.97
113_S	132_H	0.952	0.97
86_K	105_N	0.951	0.96
395_A	417_L	0.949	0.96
389_N	430_K	0.948	0.96
179_I	183_K	0.944	0.96
43_P	47_Q	0.943	0.96
417_L	425_L	0.941	0.96
71_Q	75_H	0.938	0.96
70_R	111_W	0.938	0.96
151_L	154_R	0.936	0.96
181_R	184_W	0.935	0.96
395_A	423_G	0.933	0.96
161_D	164_N	0.929	0.96
281_I	289_D	0.926	0.96
57_L	91_K	0.926	0.96
113_S	117_L	0.923	0.96
48_E	202_W	0.923	0.96
62_F	110_A	0.918	0.96
375_A	378_Y	0.914	0.96
263_L	421_L	0.910	0.95
342_Y	427_I	0.906	0.95
387_E	390_T	0.904	0.95
157_R	170_K	0.902	0.95
40_R	203_Y	0.901	0.95
247_L	368_M	0.901	0.95
400_E	403_R	0.900	0.95
41_L	204_A	0.899	0.95
66_N	111_W	0.898	0.95
179_I	184_W	0.898	0.95
236_K	239_G	0.894	0.95
243_A	256_V	0.891	0.95
281_I	285_Q	0.883	0.95
330_I	341_I	0.882	0.95
402_K	409_I	0.881	0.95
406_R	419_S	0.881	0.95
310_L	314_L	0.879	0.95
254_S	263_L	0.879	0.95
404_N	419_S	0.876	0.94
174_M	182_L	0.875	0.94
371_I	382_V	0.872	0.94
393_Y	426_N	0.871	0.94
319_T	370_S	0.870	0.94
163_E	166_K	0.870	0.94
166_K	175_E	0.869	0.94
98_V	102_A	0.866	0.94
253_A	325_L	0.866	0.94
97_G	157_R	0.866	0.94
161_D	168_I	0.862	0.94
101_Q	105_N	0.862	0.94
409_I	419_S	0.857	0.94
374_K	377_E	0.854	0.94
277_F	310_L	0.849	0.93
266_K	269_R	0.848	0.93
73_F	77_G	0.846	0.93
192_E	205_H	0.845	0.93
321_L	325_L	0.844	0.93
275_F	357_F	0.843	0.93
300_Y	304_L	0.841	0.93
244_S	428_L	0.841	0.93
292_R	295_G	0.838	0.93
60_K	64_E	0.837	0.93
174_M	181_R	0.837	0.93
67_F	138_Y	0.835	0.93
259_D	328_H	0.835	0.93
375_A	380_M	0.835	0.93
153_I	156_D	0.828	0.93
406_R	409_I	0.823	0.92
168_I	175_E	0.821	0.92
246_D	420_D	0.818	0.92
104_L	108_N	0.813	0.92
167_L	191_L	0.811	0.92
282_A	287_L	0.811	0.92
68_E	72_Q	0.810	0.92
72_Q	76_E	0.810	0.92
98_V	155_Q	0.808	0.92
283_E	287_L	0.808	0.92
313_K	317_R	0.807	0.92
98_V	101_Q	0.806	0.92
146_K	149_Q	0.806	0.92
152_V	190_R	0.805	0.92
251_V	265_Y	0.804	0.91
69_R	85_Y	0.801	0.91
330_I	380_M	0.801	0.91
400_E	406_R	0.801	0.91
102_A	154_R	0.798	0.91
395_A	415_H	0.797	0.91
117_L	132_H	0.797	0.91
176_I	180_G	0.791	0.91
399_V	412_P	0.789	0.91
186_G	208_I	0.788	0.91
80_N	86_K	0.782	0.90
288_A	291_T	0.782	0.90
251_V	263_L	0.780	0.90
430_K	434_V	0.778	0.90
345_Y	387_E	0.772	0.90
153_I	157_R	0.770	0.90
299_A	302_E	0.768	0.90
111_W	114_F	0.768	0.90
97_G	156_D	0.766	0.89
408_V	417_L	0.765	0.89
392_K	400_E	0.765	0.89
193_I	204_A	0.763	0.89
265_Y	268_V	0.761	0.89
354_G	358_T	0.759	0.89
98_V	157_R	0.759	0.89
57_L	92_Y	0.758	0.89
56_N	59_A	0.758	0.89
369_E	372_E	0.753	0.89
149_Q	152_V	0.752	0.89
78_K	84_T	0.751	0.88
152_V	192_E	0.751	0.88
332_T	339_S	0.749	0.88
327_S	374_K	0.749	0.88
291_T	296_E	0.748	0.88
249_I	361_M	0.748	0.88
301_L	305_R	0.748	0.88
365_R	369_E	0.743	0.88
282_A	285_Q	0.742	0.88
157_R	160_V	0.741	0.88
393_Y	400_E	0.738	0.88
345_Y	368_M	0.738	0.88
169_L	172_F	0.737	0.88
36_T	194_I	0.735	0.87
38_V	194_I	0.733	0.87
39_V	204_A	0.733	0.87
62_F	107_N	0.732	0.87
355_N	359_V	0.731	0.87
348_N	387_E	0.729	0.87
253_A	367_L	0.729	0.87
175_E	180_G	0.727	0.87
190_R	207_P	0.724	0.87
95_I	98_V	0.724	0.87
280_R	284_V	0.722	0.86
56_N	60_K	0.719	0.86
61_L	103_V	0.719	0.86
289_D	303_L	0.718	0.86
187_K	209_E	0.717	0.86
256_V	421_L	0.715	0.86
340_T	381_K	0.712	0.86
80_N	83_E	0.711	0.86
286_S	289_D	0.710	0.86
243_A	338_V	0.710	0.86
102_A	152_V	0.710	0.86
349_I	358_T	0.709	0.85
238_K	241_K	0.709	0.85
243_A	341_I	0.708	0.85
370_S	374_K	0.704	0.85
393_Y	409_I	0.701	0.85
423_G	426_N	0.700	0.85
57_L	95_I	0.700	0.85
346_P	387_E	0.700	0.85
177_D	180_G	0.700	0.85
156_D	159_K	0.698	0.85
244_S	256_V	0.698	0.85
159_K	168_I	0.698	0.85
80_N	84_T	0.696	0.84
195_F	202_W	0.696	0.84
34_R	207_P	0.695	0.84
117_L	134_S	0.695	0.84
409_I	412_P	0.693	0.84
97_G	154_R	0.692	0.84
76_E	79_V	0.690	0.84
106_K	192_E	0.689	0.84
51_L	55_A	0.689	0.84
274_Y	277_F	0.688	0.84
34_R	189_G	0.683	0.83
54_L	151_L	0.682	0.83
392_K	403_R	0.681	0.83
103_V	107_N	0.680	0.83
322_Y	363_S	0.679	0.83
151_L	155_Q	0.675	0.83
291_T	299_A	0.671	0.82
153_I	158_Y	0.670	0.82
95_I	100_A	0.669	0.82
56_N	91_K	0.668	0.82
73_F	114_F	0.668	0.82
387_E	430_K	0.666	0.82
383_F	431_A	0.666	0.82
38_V	319_T	0.665	0.82
368_M	372_E	0.664	0.82
53_R	57_L	0.663	0.81
236_K	380_M	0.663	0.81
402_K	407_G	0.663	0.81
246_D	391_S	0.662	0.81
151_L	157_R	0.662	0.81
69_R	111_W	0.661	0.81
80_N	85_Y	0.661	0.81
427_I	430_K	0.658	0.81
73_F	76_E	0.657	0.81
331_E	340_T	0.656	0.81
296_E	299_A	0.656	0.81
111_W	115_F	0.654	0.81
300_Y	303_L	0.653	0.80
421_L	424_A	0.653	0.80
99_N	151_L	0.651	0.80
282_A	290_R	0.649	0.80
54_L	95_I	0.649	0.80
201_A	316_R	0.647	0.80
64_E	91_K	0.646	0.80
240_N	339_S	0.642	0.79
73_F	111_W	0.639	0.79
54_L	96_L	0.633	0.78
249_I	357_F	0.632	0.78
340_T	383_F	0.630	0.78
168_I	172_F	0.629	0.78
395_A	429_K	0.627	0.78
401_V	406_R	0.627	0.78
96_L	153_I	0.626	0.78
275_F	356_K	0.625	0.77
41_L	193_I	0.625	0.77
282_A	307_K	0.625	0.77
36_T	207_P	0.625	0.77
250_N	263_L	0.624	0.77
393_Y	398_D	0.624	0.77
396_Y	417_L	0.624	0.77
102_A	106_K	0.624	0.77
168_I	171_D	0.623	0.77
292_R	296_E	0.619	0.77
274_Y	356_K	0.618	0.77
244_S	342_Y	0.618	0.77
289_D	307_K	0.618	0.77
352_E	359_V	0.618	0.77
396_Y	425_L	0.616	0.76
431_A	435_I	0.615	0.76
408_V	418_H	0.615	0.76
280_R	310_L	0.613	0.76
287_L	290_R	0.611	0.76
316_R	319_T	0.610	0.76
259_D	264_L	0.608	0.76
389_N	393_Y	0.608	0.76
425_L	428_L	0.607	0.75
386_V	389_N	0.606	0.75
104_L	107_N	0.606	0.75
110_A	136_P	0.604	0.75
250_N	254_S	0.604	0.75
306_E	309_R	0.604	0.75
158_Y	170_K	0.603	0.75
274_Y	278_Q	0.603	0.75
275_F	278_Q	0.603	0.75
50_K	54_L	0.601	0.75
325_L	328_H	0.601	0.75
292_R	300_Y	0.601	0.75
255_V	258_N	0.600	0.75
274_Y	314_L	0.597	0.74
90_E	93_K	0.595	0.74
327_S	370_S	0.595	0.74
43_P	176_I	0.594	0.74
253_A	329_L	0.593	0.74
390_T	393_Y	0.592	0.74
175_E	184_W	0.592	0.74
341_I	371_I	0.591	0.73
107_N	111_W	0.589	0.73
169_L	191_L	0.585	0.73
285_Q	311_F	0.585	0.73
252_I	326_A	0.585	0.73
105_N	154_R	0.584	0.73
285_Q	288_A	0.584	0.73
68_E	71_Q	0.583	0.72
304_L	308_R	0.583	0.72
61_L	100_A	0.583	0.72
429_K	434_V	0.583	0.72
177_D	182_L	0.581	0.72
311_F	318_L	0.579	0.72
79_V	84_T	0.579	0.72
150_I	153_I	0.577	0.72
203_Y	312_K	0.576	0.72
60_K	89_Y	0.575	0.71
249_I	350_V	0.574	0.71
277_F	313_K	0.574	0.71
252_I	322_Y	0.572	0.71
404_N	408_V	0.569	0.71
194_I	198_T	0.569	0.71
54_L	99_N	0.567	0.70
353_K	357_F	0.566	0.70
418_H	423_G	0.566	0.70
348_N	351_Q	0.565	0.70
78_K	81_I	0.563	0.70
114_F	117_L	0.560	0.69
115_F	136_P	0.559	0.69
293_N	300_Y	0.557	0.69
348_N	352_E	0.557	0.69
51_L	151_L	0.556	0.69
389_N	409_I	0.554	0.69
252_I	371_I	0.553	0.69
175_E	179_I	0.552	0.68
417_L	422_N	0.549	0.68
64_E	68_E	0.548	0.68
98_V	153_I	0.547	0.68
97_G	153_I	0.546	0.68
286_S	290_R	0.546	0.68
359_V	391_S	0.546	0.68
154_R	157_R	0.544	0.67
346_P	349_I	0.544	0.67
349_I	364_Y	0.542	0.67
68_E	85_Y	0.540	0.67
325_L	367_L	0.540	0.67
284_V	306_E	0.539	0.67
38_V	203_Y	0.539	0.67
245_I	329_L	0.539	0.67
38_V	198_T	0.539	0.67
176_I	179_I	0.537	0.66
152_V	156_D	0.534	0.66
342_Y	430_K	0.534	0.66
53_R	95_I	0.531	0.65
101_Q	104_L	0.530	0.65
208_I	331_E	0.524	0.64
348_N	358_T	0.524	0.64
182_L	186_G	0.523	0.64
73_F	115_F	0.523	0.64
422_N	426_N	0.522	0.64
324_N	327_S	0.519	0.64
373_L	377_E	0.517	0.63
57_L	60_K	0.516	0.63
274_Y	317_R	0.516	0.63
81_I	85_Y	0.516	0.63
253_A	264_L	0.515	0.63
79_V	108_N	0.515	0.63
285_Q	293_N	0.515	0.63
54_L	58_S	0.515	0.63
412_P	419_S	0.514	0.63
249_I	268_V	0.512	0.63
51_L	193_I	0.512	0.63
388_Y	392_K	0.511	0.62
403_R	409_I	0.510	0.62
58_S	95_I	0.509	0.62
116_S	133_V	0.509	0.62
55_A	151_L	0.508	0.62
147_R	150_I	0.507	0.62
69_R	72_Q	0.506	0.62
253_A	343_L	0.505	0.62
241_K	339_S	0.504	0.61
177_D	184_W	0.504	0.61
269_R	317_R	0.504	0.61
314_L	318_L	0.501	0.61
189_G	207_P	0.500	0.61

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1nu0A	1	0.2826	95.7	0.934	Contact Map
1iv0A	1	0.2109	94.3	0.939	Contact Map
1vhxA	2	0.2978	94.2	0.939	Contact Map
4ep4A	2	0.3391	75.6	0.955	Contact Map
1qypA	1	0.0935	71.9	0.956	Contact Map
4aybP	1	0.087	66.7	0.958	Contact Map
2k4xA	1	0.1152	53.9	0.961	Contact Map
1hjrA	2	0.3348	51.1	0.962	Contact Map
1ffkW	1	0.1522	50.9	0.962	Contact Map
3j20Y	1	0.1043	41.8	0.964	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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