Q7LX51 - SULSO - GREMLIN Contacts

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Q7LX51 - Transposase ISC1217

UniProt:	Q7LX51	Sequences: MTKELTREEYYKALEKAVNEVILSMTGTRKDVAKRLLLGAVVGRNATEIAQEAEMDYETVLNNLDKAAQAKLIEVVKKLVGDHPVLLIIDDTHDHKLYARAMPVSRNGAQIFYCRAHKRFEPAIQLLVIGVKDLVNNQIYVIHIIAYIPRKVEEELKRRGEEVKFKTKIDALLEFLSSLSGLNVKARVFDSWYVNSRTLQGNTVGELKSSARVVEGGRSVPVSEFPQGEYLVEYLGTPIKLLVIDDYKGYGRRYFFSTDLNDTAEDIITTWENRWDIEVLIRELKALGLEGGSFLTWVRNSGFVALKALSLLVVQYFKYSTGLMLGAKRLARLIKSIYREAGGIKKLFKRRRKP	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	354 (299)
Sequences:	1058 (821)
Seq/√Len:	47.5

SULSO - Q7LX51 Q7LYK0 Q97VQ0 Q97XR1 Q97ZD8 Q97Y34 Q97YE4 Q97XK5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
189_F	205_G	4.597	1.00
145_I	178_S	3.509	1.00
36_L	303_F	3.317	1.00
89_I	172_L	3.259	1.00
287_L	307_K	3.169	1.00
91_D	192_W	2.839	1.00
166_K	174_E	2.825	1.00
90_D	274_R	2.756	1.00
90_D	278_E	2.637	1.00
143_H	178_S	2.566	1.00
89_I	187_R	2.494	1.00
204_V	271_W	2.455	1.00
128_V	281_I	2.396	1.00
88_I	132_K	2.325	1.00
142_I	182_L	2.298	1.00
222_V	228_G	2.295	1.00
127_L	193_Y	2.273	1.00
150_R	154_E	2.228	1.00
274_R	278_E	2.175	1.00
143_H	175_F	2.137	1.00
130_G	139_I	2.065	1.00
239_I	242_L	2.037	1.00
91_D	168_K	2.025	0.99
145_I	174_E	2.022	0.99
222_V	226_P	1.988	0.99
278_E	285_K	1.980	0.99
152_V	155_E	1.926	0.99
91_D	193_Y	1.915	0.99
22_I	30_K	1.884	0.99
220_V	223_S	1.872	0.99
224_E	228_G	1.867	0.99
293_S	299_R	1.858	0.99
240_K	244_I	1.848	0.99
26_T	30_K	1.826	0.99
275_W	278_E	1.813	0.99
90_D	285_K	1.761	0.99
294_F	299_R	1.752	0.98
231_L	234_Y	1.749	0.98
62_N	65_D	1.748	0.98
32_V	59_T	1.741	0.98
36_L	40_A	1.730	0.98
193_Y	274_R	1.698	0.98
147_Y	165_F	1.676	0.98
228_G	232_V	1.654	0.98
224_E	230_Y	1.628	0.98
70_A	74_E	1.618	0.97
130_G	281_I	1.601	0.97
167_T	170_D	1.592	0.97
91_D	275_W	1.590	0.97
243_V	246_D	1.572	0.97
145_I	301_S	1.569	0.97
85_V	131_V	1.568	0.97
95_H	117_H	1.565	0.97
93_H	121_E	1.553	0.97
168_K	193_Y	1.548	0.97
221_P	228_G	1.539	0.97
170_D	173_L	1.534	0.96
86_L	270_T	1.534	0.96
115_R	192_W	1.506	0.96
22_I	25_M	1.496	0.96
226_P	229_E	1.495	0.96
211_A	256_F	1.489	0.96
63_N	292_G	1.482	0.96
220_V	229_E	1.473	0.96
296_T	307_K	1.473	0.96
129_I	175_F	1.462	0.95
93_H	114_C	1.462	0.95
64_L	306_L	1.462	0.95
90_D	275_W	1.438	0.95
203_T	258_T	1.429	0.95
274_R	285_K	1.425	0.95
194_V	205_G	1.418	0.95
158_R	298_V	1.410	0.94
89_I	129_I	1.402	0.94
275_W	285_K	1.379	0.94
45_N	50_A	1.365	0.93
260_L	264_A	1.363	0.93
206_E	253_R	1.356	0.93
303_F	310_S	1.335	0.93
77_K	179_L	1.314	0.92
108_G	288_G	1.310	0.92
193_Y	285_K	1.300	0.91
279_V	283_E	1.287	0.91
114_C	121_E	1.277	0.91
109_A	122_P	1.275	0.91
260_L	265_E	1.269	0.90
202_N	258_T	1.266	0.90
297_W	301_S	1.260	0.90
18_V	34_K	1.257	0.90
37_L	309_L	1.255	0.90
240_K	245_D	1.253	0.90
295_L	303_F	1.246	0.90
92_T	278_E	1.245	0.90
260_L	263_T	1.237	0.89
152_V	160_G	1.224	0.89
48_E	51_Q	1.221	0.89
186_A	270_T	1.216	0.88
108_G	148_I	1.210	0.88
39_G	49_I	1.209	0.88
211_A	254_Y	1.204	0.88
102_M	124_I	1.204	0.88
69_Q	302_G	1.203	0.88
21_V	25_M	1.200	0.88
240_K	243_V	1.198	0.88
22_I	29_R	1.194	0.88
239_I	243_V	1.191	0.87
19_N	23_L	1.191	0.87
188_V	270_T	1.189	0.87
292_G	300_N	1.184	0.87
154_E	162_E	1.182	0.87
130_G	280_L	1.182	0.87
242_L	267_I	1.172	0.87
220_V	232_V	1.168	0.86
20_E	25_M	1.163	0.86
262_D	265_E	1.160	0.86
276_D	311_L	1.156	0.86
200_Q	214_V	1.153	0.86
58_E	62_N	1.148	0.85
168_K	192_W	1.142	0.85
205_G	256_F	1.135	0.85
86_L	188_V	1.132	0.85
93_H	112_F	1.129	0.84
43_G	46_A	1.129	0.84
145_I	171_A	1.128	0.84
204_V	257_S	1.119	0.84
190_D	278_E	1.108	0.83
242_L	246_D	1.101	0.83
222_V	227_Q	1.096	0.83
304_V	307_K	1.092	0.82
201_G	258_T	1.091	0.82
274_R	281_I	1.090	0.82
21_V	24_S	1.089	0.82
90_D	277_I	1.089	0.82
228_G	231_L	1.086	0.82
57_Y	61_L	1.083	0.82
96_K	109_A	1.077	0.81
139_I	276_D	1.076	0.81
92_T	275_W	1.076	0.81
222_V	225_F	1.076	0.81
242_L	252_R	1.075	0.81
141_V	280_L	1.074	0.81
195_N	210_S	1.073	0.81
147_Y	168_K	1.070	0.81
275_W	314_V	1.062	0.81
94_D	126_L	1.060	0.80
34_K	38_L	1.059	0.80
230_Y	233_E	1.054	0.80
221_P	226_P	1.054	0.80
114_C	117_H	1.051	0.80
127_L	168_K	1.049	0.80
221_P	227_Q	1.046	0.80
29_R	67_A	1.037	0.79
46_A	293_S	1.030	0.78
127_L	172_L	1.030	0.78
139_I	280_L	1.028	0.78
112_F	115_R	1.024	0.78
114_C	125_Q	1.021	0.78
125_Q	168_K	1.013	0.77
90_D	193_Y	1.012	0.77
285_K	314_V	1.009	0.77
167_T	177_S	1.003	0.77
28_T	33_A	1.003	0.77
225_F	228_G	1.001	0.76
88_I	188_V	0.999	0.76
225_F	230_Y	0.998	0.76
38_L	41_V	0.996	0.76
222_V	229_E	0.996	0.76
102_M	242_L	0.994	0.76
227_Q	231_L	0.993	0.76
63_N	67_A	0.990	0.76
216_G	219_S	0.988	0.76
224_E	229_E	0.987	0.75
128_V	141_V	0.986	0.75
220_V	225_F	0.985	0.75
207_L	256_F	0.984	0.75
63_N	69_Q	0.980	0.75
153_E	156_L	0.973	0.74
73_I	77_K	0.973	0.74
126_L	300_N	0.970	0.74
255_F	271_W	0.966	0.74
141_V	311_L	0.963	0.74
124_I	300_N	0.958	0.73
77_K	200_Q	0.955	0.73
249_G	257_S	0.951	0.73
158_R	318_K	0.949	0.73
141_V	312_L	0.949	0.73
124_I	148_I	0.946	0.72
171_A	175_F	0.941	0.72
97_L	102_M	0.935	0.71
226_P	234_Y	0.933	0.71
75_V	80_V	0.932	0.71
277_I	285_K	0.931	0.71
26_T	29_R	0.928	0.71
25_M	29_R	0.925	0.71
224_E	227_Q	0.924	0.71
24_S	71_K	0.921	0.70
243_V	247_Y	0.920	0.70
193_Y	275_W	0.919	0.70
115_R	121_E	0.917	0.70
129_I	176_L	0.910	0.69
281_I	285_K	0.908	0.69
189_F	194_V	0.907	0.69
152_V	159_R	0.904	0.69
153_E	162_E	0.904	0.69
36_L	293_S	0.903	0.69
284_L	289_L	0.899	0.68
227_Q	230_Y	0.898	0.68
112_F	124_I	0.897	0.68
126_L	304_V	0.895	0.68
82_D	136_N	0.895	0.68
127_L	171_A	0.894	0.68
160_G	163_V	0.894	0.68
306_L	310_S	0.894	0.68
33_A	37_L	0.893	0.68
222_V	230_Y	0.888	0.67
92_T	285_K	0.885	0.67
110_Q	151_K	0.880	0.67
91_D	277_I	0.880	0.67
150_R	187_R	0.872	0.66
112_F	121_E	0.870	0.66
263_T	266_D	0.866	0.65
199_L	205_G	0.866	0.65
127_L	145_I	0.866	0.65
212_R	236_G	0.862	0.65
181_G	214_V	0.861	0.65
90_D	168_K	0.860	0.65
21_V	34_K	0.860	0.65
238_P	242_L	0.860	0.65
91_D	281_I	0.853	0.64
101_A	292_G	0.851	0.64
111_I	122_P	0.845	0.64
261_N	264_A	0.844	0.63
48_E	293_S	0.844	0.63
281_I	284_L	0.842	0.63
220_V	228_G	0.841	0.63
172_L	187_R	0.841	0.63
125_Q	149_P	0.839	0.63
94_D	304_V	0.838	0.63
27_G	31_D	0.838	0.63
98_Y	109_A	0.834	0.63
94_D	128_V	0.834	0.63
207_L	278_E	0.833	0.62
58_E	72_L	0.829	0.62
116_A	290_E	0.829	0.62
117_H	121_E	0.829	0.62
32_V	35_R	0.826	0.62
191_S	207_L	0.826	0.62
229_E	232_V	0.813	0.61
23_L	30_K	0.810	0.60
92_T	274_R	0.809	0.60
190_D	274_R	0.808	0.60
192_W	275_W	0.806	0.60
271_W	274_R	0.804	0.60
98_Y	295_L	0.804	0.60
129_I	187_R	0.800	0.59
299_R	303_F	0.795	0.59
112_F	149_P	0.793	0.59
114_C	275_W	0.790	0.58
214_V	219_S	0.787	0.58
150_R	217_G	0.787	0.58
189_F	192_W	0.779	0.57
18_V	37_L	0.775	0.57
234_Y	318_K	0.774	0.57
89_I	176_L	0.772	0.57
214_V	218_R	0.771	0.56
20_E	24_S	0.771	0.56
45_N	118_K	0.768	0.56
21_V	33_A	0.765	0.56
72_L	75_V	0.752	0.55
93_H	120_F	0.752	0.55
278_E	281_I	0.749	0.54
199_L	256_F	0.749	0.54
193_Y	278_E	0.746	0.54
148_I	159_R	0.746	0.54
241_L	244_I	0.734	0.53
166_K	177_S	0.733	0.53
44_R	291_G	0.731	0.53
86_L	272_E	0.730	0.52
25_M	28_T	0.728	0.52
18_V	21_V	0.726	0.52
39_G	60_V	0.725	0.52
129_I	179_L	0.722	0.52
98_Y	101_A	0.721	0.52
152_V	157_K	0.721	0.52
21_V	27_G	0.718	0.51
90_D	190_D	0.716	0.51
115_R	120_F	0.713	0.51
278_E	314_V	0.710	0.50
32_V	63_N	0.710	0.50
92_T	289_L	0.709	0.50
148_I	152_V	0.705	0.50
17_A	20_E	0.704	0.50
54_E	218_R	0.697	0.49
194_V	198_T	0.694	0.49
221_P	224_E	0.692	0.49
156_L	162_E	0.689	0.48
17_A	313_V	0.686	0.48
277_I	287_L	0.685	0.48
60_V	63_N	0.684	0.48
127_L	147_Y	0.678	0.47
25_M	30_K	0.678	0.47
62_N	67_A	0.677	0.47
222_V	233_E	0.677	0.47
275_W	281_I	0.673	0.47
127_L	274_R	0.671	0.47
172_L	194_V	0.664	0.46
210_S	250_Y	0.663	0.46
228_G	233_E	0.661	0.46
93_H	125_Q	0.660	0.45
68_A	302_G	0.659	0.45
191_S	208_K	0.659	0.45
153_E	159_R	0.654	0.45
288_G	291_G	0.652	0.45
26_T	32_V	0.650	0.44
188_V	273_N	0.650	0.44
122_P	149_P	0.650	0.44
168_K	172_L	0.648	0.44
44_R	50_A	0.638	0.43
195_N	205_G	0.638	0.43
114_C	120_F	0.637	0.43
97_L	121_E	0.637	0.43
40_A	306_L	0.636	0.43
39_G	288_G	0.636	0.43
225_F	229_E	0.635	0.43
257_S	293_S	0.634	0.43
112_F	125_Q	0.633	0.43
228_G	234_Y	0.632	0.43
69_Q	305_A	0.631	0.43
119_R	200_Q	0.630	0.42
124_I	303_F	0.630	0.42
94_D	101_A	0.629	0.42
239_I	244_I	0.625	0.42
111_I	121_E	0.624	0.42
223_S	228_G	0.623	0.42
95_H	121_E	0.622	0.42
102_M	297_W	0.621	0.42
166_K	207_L	0.616	0.41
264_A	273_N	0.614	0.41
94_D	303_F	0.614	0.41
289_L	304_V	0.613	0.41
92_T	304_V	0.613	0.41
279_V	282_R	0.611	0.41
60_V	142_I	0.611	0.41
102_M	300_N	0.610	0.41
132_K	273_N	0.608	0.40
127_L	289_L	0.607	0.40
123_A	271_W	0.605	0.40
131_V	189_F	0.605	0.40
192_W	278_E	0.602	0.40
34_K	176_L	0.601	0.40
87_L	144_I	0.601	0.40
43_G	49_I	0.600	0.40
241_L	254_Y	0.597	0.39
245_D	248_K	0.595	0.39
17_A	31_D	0.592	0.39
153_E	161_E	0.591	0.39
29_R	296_T	0.590	0.39
299_R	306_L	0.589	0.39
220_V	226_P	0.588	0.38
21_V	213_V	0.588	0.38
32_V	62_N	0.588	0.38
80_V	166_K	0.587	0.38
93_H	115_R	0.587	0.38
197_R	251_G	0.587	0.38
65_D	299_R	0.586	0.38
89_I	233_E	0.585	0.38
156_L	163_V	0.584	0.38
292_G	303_F	0.580	0.38
203_T	268_I	0.580	0.38
33_A	36_L	0.579	0.38
45_N	305_A	0.578	0.37
83_H	233_E	0.576	0.37
68_A	84_P	0.574	0.37
88_I	276_D	0.573	0.37
114_C	290_E	0.572	0.37
46_A	113_Y	0.572	0.37
207_L	211_A	0.571	0.37
47_T	299_R	0.570	0.37
83_H	156_L	0.569	0.37
26_T	33_A	0.569	0.37
276_D	280_L	0.568	0.37
184_V	201_G	0.568	0.37
296_T	311_L	0.565	0.36
120_F	308_A	0.565	0.36
101_A	256_F	0.564	0.36
29_R	68_A	0.563	0.36
45_N	288_G	0.563	0.36
190_D	281_I	0.562	0.36
18_V	33_A	0.559	0.36
114_C	123_A	0.559	0.36
20_E	26_T	0.558	0.36
44_R	98_Y	0.558	0.36
168_K	171_A	0.558	0.36
45_N	311_L	0.557	0.36
99_A	109_A	0.552	0.35
39_G	108_G	0.551	0.35
31_D	68_A	0.550	0.35
316_Y	322_G	0.550	0.35
64_L	302_G	0.550	0.35
158_R	163_V	0.549	0.35
28_T	31_D	0.546	0.35
84_P	232_V	0.546	0.35
19_N	22_I	0.545	0.34
95_H	114_C	0.545	0.34
75_V	79_L	0.545	0.34
136_N	268_I	0.544	0.34
101_A	128_V	0.542	0.34
189_F	208_K	0.541	0.34
21_V	182_L	0.538	0.34
129_I	199_L	0.537	0.34
252_R	309_L	0.537	0.34
76_V	79_L	0.535	0.34
42_V	286_A	0.534	0.33
261_N	265_E	0.534	0.33
36_L	85_V	0.534	0.33
76_V	223_S	0.533	0.33
111_I	120_F	0.531	0.33
275_W	282_R	0.530	0.33
298_V	302_G	0.529	0.33
300_N	304_V	0.528	0.33
90_D	130_G	0.526	0.33
45_N	291_G	0.525	0.33
217_G	243_V	0.525	0.33
23_L	188_V	0.524	0.33
87_L	179_L	0.524	0.33
19_N	34_K	0.522	0.32
159_R	163_V	0.521	0.32
241_L	251_G	0.521	0.32
296_T	308_A	0.520	0.32
222_V	232_V	0.515	0.32
302_G	305_A	0.515	0.32
43_G	142_I	0.514	0.32
192_W	208_K	0.514	0.32
46_A	195_N	0.513	0.32
22_I	176_L	0.511	0.31
114_C	141_V	0.510	0.31
51_Q	187_R	0.510	0.31
123_A	203_T	0.509	0.31
29_R	33_A	0.508	0.31
111_I	253_R	0.508	0.31
36_L	39_G	0.507	0.31
274_R	287_L	0.506	0.31
24_S	33_A	0.505	0.31
17_A	21_V	0.503	0.31
88_I	130_G	0.503	0.31
40_A	284_L	0.503	0.31
91_D	191_S	0.501	0.31
282_R	285_K	0.500	0.31

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1musA	2	0.9237	99.9	0.716	Contact Map
2kkoA	2	0.3023	12.4	0.968	Contact Map
2j49A	1	0.2288	9.1	0.97	Contact Map
3owqA	1	0.226	8.2	0.971	Contact Map
3qfiA	1	0.2571	7.2	0.971	Contact Map
3mzyA	1	0.2119	7.1	0.971	Contact Map
3rqiA	2	0.1667	6.1	0.972	Contact Map
3hdeA	2	0.2797	5.9	0.972	Contact Map
1xjtA	1	0.2881	5.6	0.973	Contact Map
1xjuA	2	0.2881	5.3	0.973	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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