Q7LX34 - SULSO - GREMLIN Contacts

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Q7LX34 - Transposase ISC1058

UniProt:	Q7LX34	Sequences: MEKSKYKRDWSKYDENVITRYKLMFPFYVFEHWWDLLAEENRNARTKYKAPKEFDEFLAFLHIFLPYRAIEGVLRALEQLKIIPTSLDYSTIWKRVRNMKITFPEASDELEVIADATGISTNTGGQYIVAKWGKTRDSKFLKIEIVMDNNEFNVINAEVTSNEVESAVKTVKDLQDKGKKVKKFYGDKTYDANEVYKTGVEVVVPPKKNASTKRGHLARRKAVREFRKLGYDRWREEKGYGVRWRVESLFSAVKRTFGESVRATSFAGQVVEAKLKFWAYAWMVHLANSVVGRAPGIRV	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	299 (281)
Sequences:	319 (192)
Seq/√Len:	11.4

SULSO - Q7LX34 Q97TV4 Q97YB8 Q97Y57 Q97UJ1 Q97XJ2 Q97VP0 Q97US4 Q97WA4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
191_D	219_R	3.017	0.95
17_V	20_R	2.891	0.94
284_V	288_N	2.840	0.93
251_S	255_R	2.806	0.93
170_T	195_V	2.506	0.88
17_V	21_Y	2.489	0.87
257_F	276_K	2.481	0.87
272_E	281_A	2.451	0.87
10_W	13_Y	2.401	0.85
241_G	244_W	2.286	0.82
51_P	54_F	2.267	0.82
71_E	89_Y	2.212	0.80
240_Y	247_E	2.208	0.80
12_K	35_D	2.188	0.79
16_N	287_A	2.127	0.77
142_K	166_S	2.114	0.77
28_Y	33_W	2.111	0.77
253_V	261_V	2.104	0.77
39_E	42_R	2.095	0.76
121_T	139_K	2.076	0.76
208_K	211_S	2.049	0.75
219_R	234_W	2.037	0.74
14_D	89_Y	1.996	0.73
284_V	287_A	1.976	0.72
145_I	250_F	1.975	0.72
154_V	280_Y	1.945	0.71
60_F	279_A	1.912	0.69
65_L	193_N	1.898	0.69
244_W	250_F	1.882	0.68
159_V	253_V	1.852	0.67
24_M	206_P	1.851	0.67
130_A	135_T	1.825	0.65
42_R	46_T	1.804	0.64
240_Y	243_R	1.755	0.62
140_F	144_E	1.750	0.62
247_E	272_E	1.739	0.61
242_V	289_S	1.734	0.61
124_G	241_G	1.706	0.60
112_V	155_I	1.692	0.59
125_G	181_V	1.677	0.59
243_R	247_E	1.675	0.58
94_K	160_T	1.655	0.58
88_D	91_T	1.650	0.57
159_V	273_A	1.637	0.57
116_A	166_S	1.635	0.57
156_N	200_V	1.635	0.57
13_Y	20_R	1.626	0.56
147_M	246_V	1.614	0.56
52_K	55_D	1.601	0.55
92_I	185_Y	1.597	0.55
33_W	57_F	1.596	0.55
111_E	250_F	1.595	0.55
119_I	159_V	1.590	0.54
156_N	173_D	1.555	0.53
191_D	234_W	1.547	0.52
249_L	252_A	1.545	0.52
216_H	220_R	1.542	0.52
15_E	19_T	1.520	0.51
55_D	95_R	1.515	0.51
266_F	270_V	1.509	0.50
42_R	47_K	1.494	0.50
113_I	149_N	1.490	0.49
134_K	260_S	1.487	0.49
69_A	160_T	1.476	0.49
34_W	57_F	1.474	0.49
185_Y	203_V	1.464	0.48
159_V	270_V	1.462	0.48
213_K	241_G	1.448	0.47
192_A	195_V	1.433	0.47
146_V	150_N	1.431	0.47
202_V	222_A	1.426	0.46
284_V	289_S	1.420	0.46
90_S	233_R	1.415	0.46
206_P	210_A	1.366	0.43
88_D	202_V	1.362	0.43
122_N	139_K	1.354	0.43
252_A	280_Y	1.351	0.43
169_K	172_K	1.343	0.42
51_P	59_A	1.339	0.42
152_F	249_L	1.338	0.42
31_E	40_E	1.335	0.42
76_A	127_Y	1.334	0.42
13_Y	17_V	1.333	0.42
123_T	126_Q	1.330	0.42
114_A	174_L	1.320	0.41
166_S	281_A	1.320	0.41
95_R	234_W	1.311	0.41
191_D	207_K	1.301	0.40
265_S	268_G	1.300	0.40
81_K	143_I	1.300	0.40
273_A	284_V	1.294	0.40
63_I	83_I	1.287	0.40
67_Y	80_L	1.282	0.39
29_V	202_V	1.282	0.39
95_R	191_D	1.281	0.39
161_S	246_V	1.279	0.39
11_S	29_V	1.273	0.39
34_W	41_N	1.270	0.39
102_T	236_E	1.266	0.39
195_V	213_K	1.259	0.38
212_T	223_V	1.253	0.38
6_Y	14_D	1.247	0.38
52_K	145_I	1.246	0.38
259_E	276_K	1.244	0.38
188_K	207_K	1.240	0.38
128_I	235_R	1.237	0.37
29_V	33_W	1.229	0.37
264_T	289_S	1.227	0.37
119_I	273_A	1.225	0.37
151_E	241_G	1.222	0.37
76_A	95_R	1.213	0.36
65_L	149_N	1.209	0.36
52_K	95_R	1.205	0.36
232_D	235_R	1.199	0.36
114_A	139_K	1.193	0.35
8_R	33_W	1.188	0.35
148_D	189_T	1.187	0.35
33_W	40_E	1.186	0.35
50_A	108_D	1.177	0.35
111_E	149_N	1.166	0.34
58_L	74_L	1.164	0.34
74_L	155_I	1.158	0.34
19_T	25_F	1.156	0.34
39_E	47_K	1.152	0.34
124_G	242_V	1.148	0.33
6_Y	155_I	1.143	0.33
24_M	50_A	1.142	0.33
6_Y	16_N	1.141	0.33
162_N	224_R	1.138	0.33
73_V	210_A	1.132	0.33
19_T	90_S	1.107	0.32
45_R	54_F	1.104	0.31
129_V	135_T	1.100	0.31
83_I	117_T	1.095	0.31
112_V	150_N	1.095	0.31
144_E	160_T	1.093	0.31
139_K	162_N	1.092	0.31
57_F	261_V	1.089	0.31
247_E	281_A	1.089	0.31
127_Y	144_E	1.087	0.31
43_N	184_F	1.087	0.31
124_G	128_I	1.083	0.31
28_Y	31_E	1.080	0.30
16_N	288_N	1.072	0.30
269_Q	281_A	1.072	0.30
229_L	233_R	1.071	0.30
24_M	60_F	1.061	0.30
205_P	269_Q	1.060	0.30
56_E	96_V	1.048	0.29
9_D	21_Y	1.047	0.29
95_R	219_R	1.045	0.29
243_R	254_K	1.043	0.29
107_S	164_V	1.043	0.29
204_V	207_K	1.041	0.29
48_Y	242_V	1.037	0.29
245_R	249_L	1.034	0.29
36_L	49_K	1.026	0.28
270_V	279_A	1.024	0.28
18_I	71_E	1.023	0.28
33_W	36_L	1.022	0.28
90_S	209_N	1.022	0.28
128_I	135_T	1.022	0.28
247_E	254_K	1.019	0.28
225_E	229_L	1.016	0.28
159_V	225_E	1.015	0.28
16_N	284_V	0.997	0.27
185_Y	285_H	0.981	0.27
114_A	161_S	0.980	0.26
6_Y	104_P	0.979	0.26
43_N	46_T	0.979	0.26
11_S	39_E	0.977	0.26
274_K	277_F	0.976	0.26
152_F	252_A	0.974	0.26
86_S	141_L	0.973	0.26
24_M	143_I	0.972	0.26
165_E	168_V	0.967	0.26
30_F	47_K	0.966	0.26
118_G	142_K	0.963	0.26
16_N	117_T	0.962	0.26
75_R	208_K	0.961	0.26
23_L	31_E	0.960	0.26
137_D	219_R	0.953	0.25
148_D	207_K	0.952	0.25
19_T	22_K	0.949	0.25
248_S	251_S	0.949	0.25
116_A	173_D	0.939	0.25
140_F	205_P	0.938	0.25
184_F	195_V	0.934	0.25
114_A	285_H	0.933	0.25
29_V	38_A	0.916	0.24
132_W	140_F	0.914	0.24
7_K	226_F	0.910	0.24
29_V	34_W	0.904	0.24
184_F	200_V	0.903	0.24
25_F	90_S	0.896	0.23
49_K	154_V	0.895	0.23
114_A	170_T	0.892	0.23
12_K	38_A	0.892	0.23
111_E	289_S	0.882	0.23
89_Y	143_I	0.874	0.23
174_L	263_A	0.874	0.23
128_I	245_R	0.872	0.23
11_S	229_L	0.871	0.23
111_E	124_G	0.864	0.22
232_D	236_E	0.863	0.22
29_V	40_E	0.861	0.22
199_G	221_K	0.854	0.22
280_Y	283_M	0.853	0.22
152_F	257_F	0.851	0.22
123_T	154_V	0.849	0.22
73_V	97_R	0.844	0.22
14_D	90_S	0.838	0.21
31_E	261_V	0.833	0.21
71_E	75_R	0.824	0.21
23_L	73_V	0.824	0.21
13_Y	115_D	0.824	0.21
184_F	202_V	0.823	0.21
107_S	246_V	0.820	0.21
274_K	284_V	0.820	0.21
144_E	166_S	0.819	0.21
16_N	62_H	0.818	0.21
62_H	202_V	0.818	0.21
273_A	287_A	0.817	0.21
15_E	91_T	0.817	0.21
9_D	17_V	0.815	0.21
127_Y	140_F	0.814	0.21
90_S	214_R	0.807	0.20
273_A	285_H	0.807	0.20
118_G	140_F	0.803	0.20
8_R	18_I	0.796	0.20
39_E	197_K	0.795	0.20
172_K	208_K	0.783	0.20
181_V	268_G	0.781	0.20
175_Q	180_K	0.781	0.20
119_I	261_V	0.779	0.20
90_S	219_R	0.779	0.20
70_I	227_R	0.775	0.19
8_R	58_L	0.775	0.19
269_Q	272_E	0.774	0.19
143_I	206_P	0.773	0.19
248_S	289_S	0.773	0.19
109_E	245_R	0.757	0.19
18_I	35_D	0.753	0.19
97_R	265_S	0.752	0.19
120_S	132_W	0.751	0.19
24_M	33_W	0.749	0.19
25_F	58_L	0.748	0.19
119_I	252_A	0.745	0.19
86_S	165_E	0.741	0.18
228_K	265_S	0.739	0.18
72_G	95_R	0.736	0.18
152_F	248_S	0.734	0.18
35_D	38_A	0.732	0.18
25_F	83_I	0.730	0.18
167_A	229_L	0.726	0.18
241_G	253_V	0.726	0.18
214_R	267_A	0.724	0.18
203_V	285_H	0.719	0.18
207_K	245_R	0.717	0.18
97_R	267_A	0.715	0.18
72_G	206_P	0.715	0.18
31_E	83_I	0.713	0.18
20_R	219_R	0.712	0.18
72_G	126_Q	0.710	0.18
243_R	258_G	0.708	0.17
195_V	241_G	0.706	0.17
80_L	286_L	0.706	0.17
280_Y	284_V	0.706	0.17
50_A	121_T	0.705	0.17
206_P	226_F	0.704	0.17
66_P	69_A	0.702	0.17
242_V	248_S	0.701	0.17
81_K	256_T	0.696	0.17
240_Y	254_K	0.696	0.17
58_L	249_L	0.696	0.17
263_A	275_L	0.694	0.17
104_P	216_H	0.693	0.17
280_Y	289_S	0.692	0.17
245_R	248_S	0.689	0.17
252_A	261_V	0.686	0.17
42_R	266_F	0.684	0.17
100_K	117_T	0.682	0.17
253_V	257_F	0.681	0.17
271_V	274_K	0.680	0.17
91_T	148_D	0.678	0.17
99_M	172_K	0.677	0.17
15_E	200_V	0.676	0.17
72_G	127_Y	0.674	0.17
160_T	266_F	0.673	0.16
51_P	55_D	0.671	0.16
134_K	233_R	0.671	0.16
163_E	226_F	0.669	0.16
57_F	259_E	0.665	0.16
74_L	262_R	0.663	0.16
248_S	266_F	0.663	0.16
186_G	238_K	0.663	0.16
136_R	241_G	0.661	0.16
7_K	34_W	0.657	0.16
92_I	246_V	0.657	0.16
128_I	134_K	0.656	0.16
92_I	175_Q	0.656	0.16
49_K	172_K	0.655	0.16
16_N	286_L	0.655	0.16
123_T	132_W	0.654	0.16
32_H	35_D	0.652	0.16
278_W	282_W	0.651	0.16
83_I	154_V	0.648	0.16
106_A	204_V	0.647	0.16
21_Y	234_W	0.647	0.16
109_E	160_T	0.647	0.16
86_S	133_G	0.646	0.16
100_K	163_E	0.644	0.16
199_G	225_E	0.640	0.16
181_V	257_F	0.640	0.16
122_N	136_R	0.640	0.16
8_R	134_K	0.639	0.16
22_K	25_F	0.639	0.16
107_S	190_Y	0.638	0.16
243_R	272_E	0.637	0.16
30_F	34_W	0.636	0.16
41_N	61_L	0.633	0.15
82_I	92_I	0.633	0.15
189_T	250_F	0.631	0.15
262_R	275_L	0.628	0.15
120_S	126_Q	0.625	0.15
141_L	252_A	0.624	0.15
153_N	158_E	0.623	0.15
133_G	188_K	0.622	0.15
130_A	136_R	0.622	0.15
253_V	276_K	0.622	0.15
146_V	238_K	0.621	0.15
74_L	83_I	0.619	0.15
167_A	263_A	0.615	0.15
39_E	48_Y	0.612	0.15
132_W	195_V	0.610	0.15
165_E	244_W	0.608	0.15
125_G	207_K	0.608	0.15
11_S	232_D	0.606	0.15
56_E	104_P	0.605	0.15
107_S	223_V	0.605	0.15
92_I	109_E	0.603	0.15
115_D	118_G	0.599	0.15
50_A	191_D	0.596	0.15
159_V	261_V	0.595	0.14
120_S	140_F	0.594	0.14
123_T	141_L	0.591	0.14
265_S	285_H	0.590	0.14
51_P	181_V	0.588	0.14
261_V	273_A	0.587	0.14
218_A	262_R	0.587	0.14
18_I	75_R	0.587	0.14
20_R	234_W	0.584	0.14
44_A	70_I	0.583	0.14
10_W	20_R	0.582	0.14
50_A	57_F	0.581	0.14
72_G	76_A	0.579	0.14
119_I	253_V	0.578	0.14
80_L	276_K	0.576	0.14
100_K	202_V	0.576	0.14
115_D	131_K	0.576	0.14
279_A	283_M	0.576	0.14
98_N	228_K	0.573	0.14
262_R	277_F	0.573	0.14
128_I	185_Y	0.571	0.14
7_K	114_A	0.571	0.14
115_D	190_Y	0.568	0.14
241_G	276_K	0.568	0.14
147_M	286_L	0.567	0.14
235_R	253_V	0.567	0.14
62_H	145_I	0.565	0.14
50_A	268_G	0.561	0.14
240_Y	272_E	0.560	0.14
133_G	141_L	0.559	0.14
12_K	100_K	0.559	0.14
22_K	51_P	0.558	0.14
161_S	164_V	0.553	0.13
28_Y	157_A	0.552	0.13
126_Q	287_A	0.552	0.13
72_G	125_G	0.551	0.13
9_D	20_R	0.551	0.13
145_I	152_F	0.551	0.13
229_L	237_E	0.548	0.13
18_I	25_F	0.545	0.13
186_G	228_K	0.545	0.13
137_D	191_D	0.541	0.13
29_V	32_H	0.536	0.13
70_I	186_G	0.536	0.13
89_Y	94_K	0.536	0.13
83_I	236_E	0.536	0.13
206_P	219_R	0.535	0.13
115_D	187_D	0.534	0.13
72_G	77_L	0.533	0.13
109_E	180_K	0.531	0.13
145_I	183_K	0.529	0.13
65_L	210_A	0.528	0.13
188_K	191_D	0.527	0.13
80_L	93_W	0.527	0.13
68_R	259_E	0.527	0.13
90_S	164_V	0.527	0.13
61_L	167_A	0.526	0.13
269_Q	280_Y	0.526	0.13
9_D	13_Y	0.525	0.13
64_F	250_F	0.525	0.13
98_N	225_E	0.524	0.13
140_F	173_D	0.524	0.13
153_N	165_E	0.523	0.13
123_T	252_A	0.523	0.13
212_T	215_G	0.520	0.13
41_N	154_V	0.520	0.13
93_W	145_I	0.519	0.13
61_L	136_R	0.517	0.13
213_K	217_L	0.515	0.13
117_T	191_D	0.515	0.13
58_L	92_I	0.514	0.13
59_A	96_V	0.513	0.13
164_V	277_F	0.513	0.13
274_K	282_W	0.508	0.12
121_T	226_F	0.503	0.12
80_L	124_G	0.502	0.12
73_V	265_S	0.501	0.12
16_N	36_L	0.500	0.12
61_L	275_L	0.500	0.12

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1musA	2	0.8629	96.9	0.931	Contact Map
4fcyA	4	0.8528	86.8	0.953	Contact Map
4fw1A	2	0.5117	53.8	0.964	Contact Map
1cxqA	2	0.4448	40	0.967	Contact Map
4u7bA	2	0.8763	37.4	0.967	Contact Map
4mq3A	1	0.4181	30.6	0.969	Contact Map
1bcoA	1	0.5686	23.5	0.97	Contact Map
3l3uA	2	0.4682	19.3	0.972	Contact Map
1c6vA	4	0.4548	17.7	0.972	Contact Map
1k6yA	4	0.592	12.7	0.974	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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