RL10 - SULSO - GREMLIN Contacts

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RL10 - 50S ribosomal protein L10

UniProt:	P96039	Sequences: MKRLALALKQRKVASWKLEEVKELTELIKNSNTILIGNLEGFPADKLHEIRKKLRGKATIKVTKNTLFKIAAKNAGIDIEKLEQYLTGPNVFIFTKDNPFITNMFFENYKLRRYAMPGDKAEEEVVIPAGDTGMPAGPILSVFGKLKVQTKVQDGKVHVVKDTVVAKPGDVIPAEALPILQKLGIMPVYVKLKIKVAYHEGLVIPAESLKLDLEGYRSNITEAYRNAFTLAVEIAYPTPDVLKFTISKVFKNAIALASEIGYITPESAQAVISKAVAKAYALATAIGGKVDLGVQLPTVQQSQSQQPAAEEKKEEKKEEEKKGPSEEEIASGLASLFG	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	338 (295)
Sequences:	578 (355)
Seq/√Len:	20.7

HALSA - RL10
SULSO - RL10

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
129_A	162_D	5.206	1.00
257_A	267_S	4.559	1.00
308_A	312_K	4.444	1.00
173_P	176_A	3.744	1.00
131_D	156_K	3.556	1.00
57_K	105_F	3.256	1.00
305_Q	308_A	3.228	1.00
32_N	96_K	3.107	1.00
275_A	279_A	3.022	0.99
27_L	59_T	2.964	0.99
311_E	315_E	2.945	0.99
272_I	279_A	2.923	0.99
15_S	19_E	2.843	0.99
138_P	182_K	2.796	0.99
120_K	171_V	2.634	0.98
128_P	162_D	2.609	0.98
88_G	95_T	2.601	0.98
309_A	312_K	2.485	0.97
312_K	315_E	2.477	0.97
305_Q	309_A	2.467	0.97
263_I	268_A	2.327	0.96
26_E	30_N	2.215	0.95
284_T	287_G	2.213	0.95
124_E	168_P	2.136	0.94
39_L	62_V	2.124	0.94
308_A	314_E	2.112	0.94
309_A	313_K	2.024	0.92
127_I	162_D	2.024	0.92
96_K	223_A	1.970	0.91
303_Q	307_P	1.877	0.89
14_A	230_L	1.863	0.88
85_Y	196_V	1.845	0.88
272_I	276_V	1.844	0.88
301_Q	306_Q	1.840	0.88
302_S	306_Q	1.831	0.87
233_E	259_E	1.802	0.87
227_A	230_L	1.759	0.85
273_S	280_Y	1.754	0.85
308_A	313_K	1.736	0.84
164_V	167_K	1.659	0.82
269_Q	273_S	1.658	0.81
303_Q	306_Q	1.651	0.81
289_K	300_Q	1.627	0.80
286_I	290_V	1.611	0.80
282_L	286_I	1.589	0.79
254_I	270_A	1.586	0.78
286_I	291_D	1.585	0.78
35_L	194_I	1.578	0.78
266_E	269_Q	1.575	0.78
309_A	314_E	1.560	0.77
229_T	233_E	1.555	0.77
143_F	150_T	1.553	0.77
305_Q	312_K	1.550	0.77
285_A	288_G	1.548	0.77
300_Q	304_S	1.543	0.76
71_A	138_P	1.529	0.76
22_K	26_E	1.500	0.74
306_Q	314_E	1.497	0.74
267_S	270_A	1.496	0.74
306_Q	312_K	1.481	0.73
299_V	307_P	1.480	0.73
211_L	257_A	1.464	0.73
82_L	247_S	1.454	0.72
28_I	60_I	1.445	0.71
301_Q	312_K	1.439	0.71
301_Q	307_P	1.428	0.71
44_A	172_I	1.423	0.70
45_D	49_E	1.413	0.70
276_V	280_Y	1.413	0.70
291_D	307_P	1.407	0.69
257_A	262_Y	1.403	0.69
14_A	18_L	1.403	0.69
300_Q	305_Q	1.400	0.69
31_S	59_T	1.396	0.69
243_K	246_I	1.383	0.68
123_E	168_P	1.379	0.68
42_F	46_K	1.370	0.67
126_V	162_D	1.354	0.66
272_I	280_Y	1.337	0.65
94_F	148_V	1.334	0.65
305_Q	311_E	1.327	0.65
115_A	172_I	1.321	0.64
167_K	170_D	1.313	0.64
126_V	164_V	1.313	0.64
39_L	92_F	1.303	0.63
43_P	223_A	1.297	0.63
129_A	211_L	1.289	0.62
104_M	108_N	1.282	0.62
192_L	212_D	1.278	0.62
161_K	250_F	1.260	0.61
307_P	312_K	1.260	0.61
301_Q	305_Q	1.259	0.61
19_E	22_K	1.255	0.60
145_K	175_E	1.251	0.60
220_I	224_Y	1.249	0.60
127_I	207_E	1.241	0.59
34_I	130_G	1.241	0.59
231_A	239_P	1.230	0.59
207_E	213_L	1.228	0.59
124_E	170_D	1.228	0.59
174_A	207_E	1.218	0.58
299_V	302_S	1.218	0.58
112_R	156_K	1.217	0.58
37_G	93_I	1.209	0.57
86_L	108_N	1.206	0.57
164_V	169_G	1.186	0.56
250_F	253_A	1.183	0.56
307_P	313_K	1.174	0.55
217_R	221_T	1.163	0.54
148_V	159_V	1.163	0.54
48_H	202_L	1.158	0.54
310_E	315_E	1.149	0.53
140_L	150_T	1.145	0.53
304_S	307_P	1.143	0.53
246_I	291_D	1.141	0.53
89_P	144_G	1.140	0.53
130_G	137_G	1.131	0.52
23_E	84_Q	1.117	0.51
310_E	314_E	1.087	0.49
20_E	24_L	1.084	0.49
93_I	247_S	1.081	0.49
241_V	245_T	1.077	0.49
246_I	253_A	1.071	0.48
77_I	80_E	1.071	0.48
311_E	314_E	1.067	0.48
36_I	86_L	1.066	0.48
257_A	263_I	1.064	0.48
206_A	255_A	1.062	0.48
243_K	267_S	1.058	0.47
247_S	265_P	1.056	0.47
124_E	149_Q	1.053	0.47
148_V	163_T	1.048	0.47
64_K	190_V	1.038	0.46
73_K	214_E	1.036	0.46
73_K	126_V	1.033	0.46
109_Y	157_V	1.033	0.46
264_T	267_S	1.032	0.46
20_E	63_T	1.025	0.45
175_E	255_A	1.022	0.45
275_A	278_K	1.018	0.45
14_A	284_T	1.011	0.44
194_I	225_R	1.010	0.44
137_G	147_K	1.007	0.44
57_K	97_D	1.005	0.44
45_D	48_H	1.004	0.44
272_I	275_A	1.003	0.44
151_K	160_V	1.002	0.44
26_E	302_S	1.001	0.44
215_G	218_S	0.999	0.44
19_E	214_E	0.992	0.43
138_P	153_Q	0.988	0.43
25_T	74_N	0.984	0.43
308_A	315_E	0.978	0.42
85_Y	203_V	0.977	0.42
18_L	234_I	0.976	0.42
250_F	254_I	0.972	0.42
156_K	182_K	0.966	0.41
42_F	72_A	0.964	0.41
103_N	210_K	0.962	0.41
39_L	47_L	0.959	0.41
256_L	259_E	0.954	0.41
26_E	116_M	0.947	0.40
84_Q	220_I	0.946	0.40
283_A	291_D	0.945	0.40
300_Q	312_K	0.938	0.40
71_A	181_Q	0.937	0.40
113_R	144_G	0.929	0.39
52_K	249_V	0.929	0.39
241_V	244_F	0.928	0.39
101_I	256_L	0.925	0.39
183_L	242_L	0.924	0.39
26_E	218_S	0.923	0.39
299_V	311_E	0.918	0.38
114_Y	191_K	0.914	0.38
144_G	152_V	0.912	0.38
106_F	190_V	0.911	0.38
42_F	145_K	0.903	0.38
67_L	183_L	0.901	0.37
133_G	156_K	0.899	0.37
93_I	290_V	0.899	0.37
46_K	113_R	0.893	0.37
309_A	315_E	0.893	0.37
70_I	147_K	0.892	0.37
274_K	277_A	0.887	0.37
217_R	224_Y	0.884	0.36
116_M	311_E	0.881	0.36
116_M	207_E	0.871	0.36
306_Q	315_E	0.863	0.35
303_Q	313_K	0.863	0.35
186_M	204_I	0.862	0.35
281_A	284_T	0.860	0.35
303_Q	312_K	0.853	0.34
300_Q	308_A	0.850	0.34
91_V	158_H	0.849	0.34
133_G	141_S	0.849	0.34
36_I	73_K	0.848	0.34
272_I	278_K	0.847	0.34
96_K	160_V	0.845	0.34
282_L	299_V	0.844	0.34
71_A	74_N	0.835	0.33
26_E	96_K	0.835	0.33
276_V	279_A	0.834	0.33
133_G	162_D	0.833	0.33
38_N	247_S	0.827	0.33
166_A	212_D	0.825	0.33
274_K	278_K	0.825	0.33
299_V	312_K	0.825	0.33
144_G	210_K	0.820	0.33
310_E	313_K	0.817	0.32
15_S	85_Y	0.815	0.32
114_Y	273_S	0.814	0.32
70_I	160_V	0.812	0.32
251_K	255_A	0.808	0.32
220_I	223_A	0.807	0.32
227_A	240_D	0.806	0.32
95_T	131_D	0.805	0.32
36_I	159_V	0.796	0.31
82_L	214_E	0.794	0.31
66_T	206_A	0.792	0.31
300_Q	303_Q	0.792	0.31
93_I	106_F	0.792	0.31
277_A	280_Y	0.790	0.31
165_V	176_A	0.790	0.31
95_T	156_K	0.783	0.30
37_G	91_V	0.783	0.30
228_F	241_V	0.781	0.30
249_V	254_I	0.778	0.30
305_Q	313_K	0.776	0.30
165_V	267_S	0.773	0.30
15_S	169_G	0.770	0.30
248_K	252_N	0.769	0.30
90_N	157_V	0.769	0.30
60_I	195_K	0.768	0.30
303_Q	308_A	0.765	0.29
257_A	260_I	0.764	0.29
246_I	280_Y	0.762	0.29
90_N	195_K	0.761	0.29
166_A	170_D	0.756	0.29
88_G	204_I	0.756	0.29
231_A	256_L	0.755	0.29
307_P	310_E	0.753	0.29
140_L	144_G	0.750	0.29
146_L	173_P	0.746	0.28
34_I	60_I	0.744	0.28
204_I	221_T	0.742	0.28
171_V	199_H	0.740	0.28
96_K	204_I	0.738	0.28
302_S	305_Q	0.733	0.28
82_L	191_K	0.732	0.28
28_I	255_A	0.732	0.28
82_L	101_I	0.732	0.28
283_A	287_G	0.730	0.28
46_K	157_V	0.728	0.27
114_Y	197_A	0.723	0.27
288_G	302_S	0.721	0.27
155_G	187_P	0.717	0.27
146_L	158_H	0.710	0.27
307_P	311_E	0.709	0.26
135_P	149_Q	0.703	0.26
42_F	250_F	0.701	0.26
60_I	96_K	0.695	0.26
254_I	267_S	0.693	0.26
72_A	76_G	0.693	0.26
122_E	143_F	0.692	0.26
127_I	185_I	0.691	0.26
65_N	237_P	0.691	0.26
145_K	172_I	0.691	0.26
81_K	84_Q	0.691	0.26
157_V	160_V	0.690	0.26
194_I	258_S	0.690	0.26
157_V	162_D	0.689	0.25
162_D	268_A	0.688	0.25
213_L	217_R	0.686	0.25
150_T	179_I	0.685	0.25
148_V	151_K	0.683	0.25
70_I	206_A	0.682	0.25
102_T	202_L	0.682	0.25
166_A	174_A	0.681	0.25
20_E	190_V	0.681	0.25
281_A	290_V	0.679	0.25
49_E	53_K	0.679	0.25
44_A	163_T	0.679	0.25
134_M	184_G	0.677	0.25
125_V	262_Y	0.673	0.25
49_E	52_K	0.672	0.25
304_S	309_A	0.669	0.24
41_G	119_D	0.667	0.24
274_K	280_Y	0.666	0.24
73_K	198_Y	0.664	0.24
28_I	116_M	0.663	0.24
64_K	103_N	0.661	0.24
154_D	214_E	0.661	0.24
123_E	129_A	0.659	0.24
36_I	57_K	0.658	0.24
198_Y	215_G	0.658	0.24
30_N	168_P	0.657	0.24
18_L	22_K	0.653	0.24
306_Q	309_A	0.652	0.24
70_I	115_A	0.650	0.24
56_G	59_T	0.648	0.23
71_A	110_K	0.648	0.23
113_R	189_Y	0.647	0.23
97_D	192_L	0.645	0.23
172_I	175_E	0.642	0.23
26_E	304_S	0.641	0.23
254_I	258_S	0.640	0.23
199_H	204_I	0.639	0.23
77_I	81_K	0.638	0.23
91_V	273_S	0.634	0.23
88_G	231_A	0.634	0.23
129_A	145_K	0.632	0.23
162_D	215_G	0.630	0.23
39_L	68_F	0.630	0.23
46_K	201_G	0.630	0.23
41_G	63_T	0.627	0.22
130_G	153_Q	0.627	0.22
42_F	111_L	0.626	0.22
299_V	308_A	0.624	0.22
206_A	213_L	0.624	0.22
250_F	266_E	0.623	0.22
54_L	134_M	0.620	0.22
26_E	309_A	0.620	0.22
304_S	308_A	0.619	0.22
303_Q	309_A	0.619	0.22
21_V	67_L	0.618	0.22
37_G	41_G	0.618	0.22
79_I	117_P	0.617	0.22
57_K	102_T	0.617	0.22
13_V	16_W	0.617	0.22
27_L	94_F	0.616	0.22
125_V	206_A	0.616	0.22
76_G	79_I	0.616	0.22
195_K	247_S	0.615	0.22
261_G	271_V	0.615	0.22
258_S	262_Y	0.615	0.22
15_S	18_L	0.614	0.22
302_S	315_E	0.614	0.22
62_V	76_G	0.614	0.22
257_A	266_E	0.611	0.22
86_L	147_K	0.610	0.22
141_S	150_T	0.610	0.22
112_R	186_M	0.610	0.22
36_I	170_D	0.608	0.22
28_I	36_I	0.608	0.22
93_I	145_K	0.606	0.22
65_N	192_L	0.606	0.22
116_M	148_V	0.606	0.22
168_P	207_E	0.605	0.21
50_I	198_Y	0.605	0.21
38_N	166_A	0.605	0.21
255_A	259_E	0.603	0.21
178_P	189_Y	0.600	0.21
117_P	160_V	0.599	0.21
99_P	243_K	0.599	0.21
157_V	299_V	0.594	0.21
28_I	76_G	0.592	0.21
36_I	224_Y	0.590	0.21
195_K	270_A	0.589	0.21
137_G	249_V	0.587	0.21
223_A	258_S	0.587	0.21
253_A	256_L	0.584	0.21
64_K	87_T	0.583	0.21
301_Q	315_E	0.582	0.20
86_L	166_A	0.580	0.20
97_D	102_T	0.579	0.20
172_I	237_P	0.579	0.20
58_A	147_K	0.578	0.20
200_E	213_L	0.577	0.20
116_M	159_V	0.577	0.20
162_D	168_P	0.576	0.20
137_G	166_A	0.576	0.20
149_Q	159_V	0.575	0.20
39_L	200_E	0.574	0.20
183_L	234_I	0.574	0.20
49_E	120_K	0.574	0.20
134_M	148_V	0.573	0.20
130_G	138_P	0.566	0.20
38_N	193_K	0.564	0.20
215_G	219_N	0.564	0.20
263_I	267_S	0.563	0.20
23_E	27_L	0.562	0.20
237_P	245_T	0.560	0.20
141_S	285_A	0.559	0.20
135_P	138_P	0.557	0.19
107_E	249_V	0.555	0.19
115_A	155_G	0.552	0.19
86_L	258_S	0.551	0.19
273_S	278_K	0.551	0.19
104_M	161_K	0.550	0.19
244_F	251_K	0.550	0.19
226_N	242_L	0.549	0.19
120_K	206_A	0.548	0.19
137_G	223_A	0.548	0.19
25_T	258_S	0.548	0.19
27_L	31_S	0.547	0.19
228_F	244_F	0.546	0.19
35_L	106_F	0.546	0.19
86_L	93_I	0.546	0.19
141_S	194_I	0.542	0.19
86_L	92_F	0.541	0.19
60_I	164_V	0.541	0.19
16_W	19_E	0.540	0.19
175_E	228_F	0.538	0.19
46_K	144_G	0.538	0.19
42_F	154_D	0.536	0.19
147_K	223_A	0.535	0.19
80_E	92_F	0.534	0.18
232_V	248_K	0.533	0.18
35_L	93_I	0.532	0.18
21_V	157_V	0.531	0.18
79_I	253_A	0.530	0.18
170_D	246_I	0.529	0.18
59_T	313_K	0.528	0.18
54_L	111_L	0.527	0.18
47_L	93_I	0.526	0.18
284_T	302_S	0.525	0.18
31_S	80_E	0.524	0.18
134_M	137_G	0.523	0.18
159_V	168_P	0.521	0.18
92_F	133_G	0.520	0.18
161_K	198_Y	0.518	0.18
97_D	272_I	0.517	0.18
32_N	218_S	0.517	0.18
19_E	29_K	0.515	0.18
32_N	181_Q	0.514	0.18
66_T	201_G	0.512	0.18
56_G	225_R	0.511	0.18
131_D	141_S	0.508	0.17
16_W	262_Y	0.507	0.17
214_E	282_L	0.505	0.17
163_T	264_T	0.505	0.17
192_L	246_I	0.505	0.17
114_Y	133_G	0.504	0.17
147_K	256_L	0.504	0.17
69_K	216_Y	0.504	0.17
20_E	61_K	0.502	0.17
214_E	218_S	0.501	0.17
252_N	269_Q	0.500	0.17
170_D	243_K	0.500	0.17
32_N	36_I	0.500	0.17
48_H	135_P	0.500	0.17
82_L	204_I	0.500	0.17

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
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HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1vq8G	1	0.003	100	0.214	Contact Map
3j21k	1	0.6095	100	0.234	Contact Map
3j3bq	1	0.8195	100	0.259	Contact Map
3j61q	1	0.7604	100	0.267	Contact Map
4ujti	1	0.4142	100	0.271	Contact Map
3a1yG	1	0.6065	100	0.307	Contact Map
4hubG	1	0.6095	100	0.38	Contact Map
3jsyA	1	0.6154	100	0.457	Contact Map
4w22q	1	0.5888	100	0.475	Contact Map
3j65n	1	0.6272	100	0.521	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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