GREMLIN Database
SYFA - Phenylalanine--tRNA ligase alpha subunit
UniProt: P95961 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 468 (452)
Sequences: 596 (392)
Seq/√Len: 18.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
78_D126_V4.8581.00
187_L192_E4.2921.00
69_K73_S3.9961.00
400_Y405_G3.7581.00
65_E68_R3.6461.00
254_Q264_H3.3401.00
52_K168_E3.3381.00
177_E197_K3.2440.99
59_K159_E3.1750.99
380_E402_E3.0690.99
68_R138_S3.0200.99
134_Y140_L2.9310.99
80_L94_I2.9200.99
282_N285_K2.9020.99
296_W301_T2.7770.98
70_R76_P2.7640.98
170_I173_P2.5310.97
378_I399_G2.5000.97
83_T98_L2.4180.96
380_E406_W2.4030.96
414_L420_L2.3760.95
386_A398_Y2.3480.95
280_L423_V2.3470.95
264_H305_M2.3450.95
68_R72_E2.3400.95
333_K453_Y2.2740.94
421_S427_S2.2400.94
362_F412_A2.2300.93
410_C434_I2.2140.93
232_L387_Y2.1600.92
395_V412_A2.1500.92
218_F437_E2.1360.92
286_E290_K2.1300.92
266_S338_D2.1220.92
388_F411_G2.1110.91
373_F390_F2.1000.91
441_M449_I2.0780.91
169_L173_P2.0750.91
399_G402_E2.0660.90
128_T131_S2.0640.90
128_T134_Y2.0610.90
268_S338_D2.0110.89
402_E405_G1.9940.89
229_M402_E1.9860.89
245_E268_S1.9840.88
57_S161_E1.9820.88
94_I359_N1.9710.88
360_F430_G1.9620.88
355_I422_S1.9300.87
218_F330_T1.8990.86
67_G71_K1.8870.86
249_F254_Q1.8580.85
202_N343_T1.8530.85
330_T352_D1.8470.84
108_W111_R1.8410.84
185_R189_I1.8360.84
180_I196_Y1.8290.84
278_K282_N1.8170.83
300_I303_R1.8050.83
289_E295_E1.8010.83
390_F437_E1.7750.82
69_K79_I1.7660.82
84_L101_D1.7660.82
382_K406_W1.7630.82
246_F284_V1.7570.81
218_F444_L1.7540.81
263_I268_S1.7420.81
109_A114_L1.7180.80
224_K443_F1.7060.79
66_E156_L1.7020.79
150_L153_K1.6840.78
397_A410_C1.6810.78
191_G194_K1.6670.78
384_K398_Y1.6570.77
231_S375_R1.6560.77
440_A452_L1.6510.77
67_G145_S1.6490.77
391_T394_S1.6290.76
39_L43_E1.5990.75
359_N386_A1.5970.75
84_L115_I1.5930.74
215_K220_E1.5850.74
42_I53_T1.5730.73
384_K388_F1.5620.73
167_V407_V1.5500.72
245_E303_R1.5410.72
245_E305_M1.5340.71
109_A115_I1.5330.71
278_K298_Q1.5190.71
281_L296_W1.5010.70
369_L397_A1.5010.70
390_F410_C1.4910.69
245_E304_L1.4900.69
54_E166_S1.4900.69
218_F345_L1.4800.69
429_A432_W1.4680.68
238_N317_V1.4670.68
437_E444_L1.4660.68
217_Y352_D1.4620.68
116_D122_V1.4520.67
225_V435_G1.4490.67
90_D120_G1.4480.67
76_P115_I1.4300.66
440_A446_I1.4280.66
193_W198_L1.4250.66
352_D390_F1.4250.66
380_E400_Y1.4210.65
369_L376_L1.4190.65
61_Y159_E1.4170.65
80_L105_A1.4110.65
66_E70_R1.4060.65
404_L407_V1.4050.64
192_E195_K1.4020.64
357_D361_T1.3920.64
420_L449_I1.3900.64
379_K402_E1.3880.63
315_A419_I1.3850.63
221_F440_A1.3830.63
237_I314_T1.3640.62
388_F396_E1.3570.62
174_K188_I1.3530.61
266_S308_S1.3500.61
361_T364_E1.3410.61
308_S338_D1.3400.61
131_S135_L1.3320.60
113_G122_V1.3240.60
110_K117_I1.3230.60
364_E367_G1.3190.59
237_I388_F1.3090.59
406_W452_L1.3080.59
79_I269_V1.3070.59
272_S314_T1.3070.59
245_E390_F1.3020.58
9_E76_P1.3000.58
106_I110_K1.2970.58
309_Q390_F1.2920.58
305_M369_L1.2890.58
340_I346_I1.2890.58
384_K396_E1.2880.58
325_P357_D1.2860.57
249_F332_G1.2790.57
132_S135_L1.2780.57
355_I430_G1.2770.57
330_T437_E1.2750.57
369_L386_A1.2670.56
224_K351_L1.2660.56
390_F434_I1.2640.56
308_S429_A1.2640.56
274_K302_L1.2550.56
191_G445_N1.2500.55
366_L397_A1.2460.55
218_F337_P1.2410.55
245_E266_S1.2400.55
254_Q263_I1.2380.54
346_I384_K1.2340.54
151_L154_R1.2330.54
66_E154_R1.2230.54
349_H437_E1.2210.53
229_M314_T1.2210.53
98_L102_F1.2200.53
259_P432_W1.2130.53
281_L298_Q1.2080.53
62_V160_K1.2070.53
411_G454_S1.2010.52
259_P393_P1.1960.52
345_L437_E1.1900.52
367_G406_W1.1870.51
337_P454_S1.1800.51
117_I120_G1.1770.51
264_H268_S1.1740.51
297_K302_L1.1710.50
135_L138_S1.1670.50
264_H309_Q1.1660.50
448_D451_L1.1630.50
266_S300_I1.1610.50
254_Q313_T1.1610.50
447_S453_Y1.1560.49
422_S434_I1.1550.49
254_Q301_T1.1510.49
43_E268_S1.1510.49
217_Y372_I1.1480.49
49_G172_E1.1470.49
399_G407_V1.1470.49
20_K60_H1.1420.49
381_V444_L1.1380.48
352_D372_I1.1280.48
188_I193_W1.1240.47
333_K382_K1.1170.47
19_L49_G1.1170.47
247_Y338_D1.1150.47
48_K91_L1.1110.47
13_L45_L1.1030.46
323_K327_K1.1030.46
218_F395_V1.0990.46
218_F351_L1.0940.46
34_P37_S1.0900.45
103_N232_L1.0900.45
85_N89_K1.0860.45
448_D456_N1.0850.45
83_T87_Q1.0810.45
301_T431_A1.0810.45
264_H338_D1.0760.44
364_E430_G1.0750.44
214_K264_H1.0730.44
253_F422_S1.0690.44
388_F454_S1.0680.44
29_I186_E1.0640.44
255_P372_I1.0570.43
83_T277_D1.0530.43
309_Q337_P1.0440.42
229_M390_F1.0430.42
437_E454_S1.0420.42
136_A143_A1.0400.42
417_P449_I1.0300.42
452_L457_I1.0280.42
131_S134_Y1.0280.42
32_G123_I1.0260.41
103_N107_S1.0230.41
165_V382_K1.0130.41
131_S137_L1.0130.41
157_I161_E1.0070.40
388_F435_G1.0070.40
80_L107_S1.0060.40
245_E300_I1.0060.40
411_G420_L1.0050.40
103_N409_M1.0050.40
237_I330_T1.0030.40
84_L262_E1.0030.40
12_I71_K1.0020.40
67_G303_R1.0010.40
24_S42_I1.0010.40
264_H308_S0.9960.40
190_S196_Y0.9950.40
390_F454_S0.9920.39
379_K382_K0.9870.39
112_K269_V0.9740.38
373_F437_E0.9730.38
49_G361_T0.9700.38
181_S305_M0.9690.38
249_F266_S0.9670.38
152_K285_K0.9660.38
218_F436_I0.9660.38
76_P103_N0.9640.38
10_A14_F0.9640.38
388_F412_A0.9600.37
235_K328_V0.9570.37
316_R320_S0.9570.37
16_L224_K0.9570.37
61_Y148_I0.9560.37
45_L176_S0.9540.37
259_P392_E0.9500.37
144_D147_T0.9500.37
14_F18_D0.9480.37
426_D440_A0.9480.37
109_A407_V0.9460.37
82_N125_K0.9450.37
104_I156_L0.9450.37
106_I117_I0.9410.36
76_P108_W0.9400.36
166_S171_K0.9380.36
263_I308_S0.9380.36
254_Q395_V0.9370.36
47_E161_E0.9360.36
414_L430_G0.9350.36
38_V378_I0.9280.36
108_W112_K0.9270.36
337_P366_L0.9270.36
143_A147_T0.9250.35
317_V432_W0.9240.35
43_E46_R0.9230.35
217_Y255_P0.9220.35
12_I41_L0.9220.35
245_E338_D0.9220.35
116_D297_K0.9200.35
244_M275_I0.9200.35
113_G367_G0.9190.35
193_W260_A0.9150.35
249_F400_Y0.9090.35
183_L239_T0.9090.35
214_K431_A0.9060.34
65_E192_E0.9050.34
337_P345_L0.9050.34
218_F352_D0.9040.34
127_K132_S0.9040.34
146_N153_K0.9000.34
351_L437_E0.9000.34
229_M264_H0.8980.34
93_E97_I0.8930.34
384_K412_A0.8920.34
162_R366_L0.8910.34
87_Q301_T0.8900.33
76_P80_L0.8850.33
395_V437_E0.8850.33
337_P388_F0.8830.33
249_F337_P0.8830.33
28_A163_K0.8820.33
330_T390_F0.8820.33
277_D445_N0.8820.33
86_G125_K0.8810.33
409_M431_A0.8760.33
412_A437_E0.8760.33
194_K283_N0.8730.33
67_G159_E0.8690.32
362_F366_L0.8680.32
221_F390_F0.8670.32
7_E364_E0.8660.32
379_K429_A0.8640.32
107_S110_K0.8630.32
94_I107_S0.8590.32
362_F395_V0.8570.32
401_L411_G0.8540.32
221_F373_F0.8510.31
337_P444_L0.8510.31
248_N254_Q0.8500.31
35_E304_L0.8480.31
332_G338_D0.8460.31
386_A420_L0.8460.31
264_H313_T0.8440.31
442_S449_I0.8430.31
384_K411_G0.8430.31
200_K369_L0.8400.31
374_Y379_K0.8360.31
222_L373_F0.8350.31
179_G358_N0.8350.31
243_E316_R0.8340.30
135_L149_N0.8330.30
9_E108_W0.8300.30
177_E196_Y0.8300.30
206_F223_E0.8290.30
29_I87_Q0.8240.30
51_V118_K0.8180.30
113_G121_K0.8170.30
12_I155_G0.8160.30
51_V319_A0.8160.30
337_P390_F0.8150.29
93_E297_K0.8130.29
12_I16_L0.8120.29
238_N332_G0.8120.29
146_N458_E0.8110.29
108_W180_I0.8100.29
245_E309_Q0.8090.29
360_F399_G0.8060.29
15_F19_L0.8060.29
83_T286_E0.8050.29
69_K125_K0.8050.29
136_A380_E0.8040.29
263_I303_R0.8040.29
243_E254_Q0.8030.29
217_Y308_S0.8030.29
264_H390_F0.8030.29
229_M453_Y0.8010.29
228_I377_G0.8000.29
155_G169_L0.7960.28
310_T377_G0.7920.28
395_V436_I0.7870.28
215_K363_K0.7870.28
218_F229_M0.7850.28
393_P404_L0.7850.28
91_L110_K0.7840.28
86_G94_I0.7840.28
222_L292_W0.7810.28
28_A35_E0.7790.28
101_D111_R0.7770.28
93_E96_N0.7740.27
66_E105_A0.7740.27
82_N100_K0.7720.27
372_I415_L0.7700.27
420_L437_E0.7690.27
322_P351_L0.7690.27
170_I222_L0.7670.27
237_I410_C0.7670.27
140_L143_A0.7640.27
346_I362_F0.7640.27
410_C415_L0.7610.27
123_I137_L0.7600.27
313_T358_N0.7580.27
72_E132_S0.7530.26
386_A396_E0.7520.26
366_L412_A0.7510.26
351_L390_F0.7510.26
101_D143_A0.7490.26
51_V374_Y0.7480.26
91_L382_K0.7480.26
200_K451_L0.7460.26
117_I161_E0.7450.26
339_A430_G0.7450.26
67_G131_S0.7450.26
43_E239_T0.7440.26
392_E432_W0.7440.26
52_K73_S0.7430.26
82_N86_G0.7420.26
377_G390_F0.7420.26
217_Y229_M0.7410.26
113_G118_K0.7400.26
283_N422_S0.7400.26
132_S139_N0.7390.26
241_Y333_K0.7390.26
24_S167_V0.7390.26
341_D344_H0.7370.26
62_V157_I0.7350.25
309_Q372_I0.7340.25
27_L51_V0.7340.25
366_L395_V0.7330.25
135_L139_N0.7320.25
19_L359_N0.7310.25
176_S275_I0.7290.25
365_L450_R0.7280.25
257_D440_A0.7240.25
213_S315_A0.7230.25
411_G435_G0.7200.25
21_K52_K0.7160.25
50_Y376_L0.7140.25
218_F349_H0.7130.24
237_I390_F0.7120.24
44_L315_A0.7120.24
23_N72_E0.7100.24
218_F390_F0.7080.24
151_L156_L0.7050.24
360_F448_D0.7040.24
337_P437_E0.7030.24
166_S188_I0.7020.24
72_E190_S0.7010.24
337_P395_V0.7010.24
78_D82_N0.7000.24
309_Q347_E0.6970.24
397_A412_A0.6960.24
381_V440_A0.6960.24
180_I187_L0.6940.24
136_A144_D0.6930.24
435_G456_N0.6910.23
94_I286_E0.6910.23
143_A194_K0.6900.23
422_S451_L0.6890.23
264_H316_R0.6890.23
134_Y137_L0.6880.23
107_S296_W0.6870.23
419_I422_S0.6850.23
82_N197_K0.6850.23
18_D405_G0.6840.23
102_F112_K0.6820.23
71_K131_S0.6820.23
267_F377_G0.6820.23
347_E441_M0.6770.23
403_K407_V0.6770.23
268_S430_G0.6760.23
81_I123_I0.6750.23
225_V436_I0.6740.23
12_I27_L0.6730.23
266_S305_M0.6730.23
143_A157_I0.6730.23
255_P309_Q0.6720.23
130_M226_K0.6720.23
38_V49_G0.6690.23
26_E111_R0.6670.22
232_L309_Q0.6670.22
102_F188_I0.6660.22
245_E251_L0.6660.22
213_S343_T0.6640.22
135_L157_I0.6630.22
272_S325_P0.6630.22
202_N207_P0.6620.22
139_N157_I0.6620.22
18_D68_R0.6600.22
112_K371_E0.6600.22
182_N381_V0.6590.22
133_E136_A0.6580.22
27_L90_D0.6560.22
77_E156_L0.6540.22
56_K166_S0.6540.22
79_I84_L0.6530.22
157_I172_E0.6520.22
401_L454_S0.6510.22
337_P346_I0.6490.22
76_P453_Y0.6490.22
390_F411_G0.6490.22
378_I384_K0.6470.22
46_R53_T0.6460.22
240_G409_M0.6450.21
409_M420_L0.6440.21
301_T447_S0.6420.21
384_K454_S0.6410.21
349_H442_S0.6410.21
270_E339_A0.6400.21
91_L107_S0.6400.21
271_G407_V0.6390.21
167_V178_I0.6390.21
133_E140_L0.6380.21
299_D302_L0.6350.21
160_K223_E0.6340.21
338_D390_F0.6330.21
398_Y409_M0.6330.21
418_E433_G0.6320.21
417_P437_E0.6300.21
215_K223_E0.6300.21
388_F398_Y0.6300.21
103_N369_L0.6290.21
305_M370_K0.6270.21
32_G157_I0.6260.21
32_G69_K0.6250.21
309_Q330_T0.6250.21
82_N290_K0.6250.21
362_F444_L0.6250.21
35_E43_E0.6250.21
327_K400_Y0.6250.21
114_L126_V0.6230.21
264_H373_F0.6180.20
172_E374_Y0.6170.20
73_S197_K0.6170.20
128_T137_L0.6150.20
275_I314_T0.6150.20
337_P411_G0.6150.20
54_E60_H0.6140.20
363_K403_K0.6140.20
322_P402_E0.6120.20
69_K181_S0.6120.20
363_K399_G0.6120.20
228_I338_D0.6120.20
33_I37_S0.6120.20
354_V388_F0.6110.20
71_K78_D0.6110.20
227_D432_W0.6110.20
11_K167_V0.6100.20
40_S44_L0.6100.20
345_L373_F0.6090.20
53_T230_I0.6090.20
11_K139_N0.6080.20
69_K190_S0.6070.20
283_N359_N0.6070.20
54_E131_S0.6050.20
331_L436_I0.6040.20
136_A140_L0.6030.20
372_I390_F0.6020.20
63_L96_N0.6000.20
28_A117_I0.5980.20
228_I310_T0.5980.20
59_K161_E0.5950.19
386_A417_P0.5940.19
417_P420_L0.5930.19
240_G313_T0.5930.19
358_N435_G0.5910.19
412_A444_L0.5910.19
17_K52_K0.5900.19
69_K138_S0.5900.19
248_N264_H0.5880.19
301_T369_L0.5840.19
111_R210_Y0.5830.19
433_G438_R0.5820.19
32_G122_V0.5810.19
283_N319_A0.5810.19
416_R433_G0.5810.19
93_E171_K0.5790.19
49_G275_I0.5790.19
245_E254_Q0.5780.19
73_S320_S0.5780.19
396_E449_I0.5780.19
302_L327_K0.5770.19
384_K397_A0.5760.19
386_A449_I0.5760.19
313_T316_R0.5750.19
145_S168_E0.5750.19
333_K381_V0.5750.19
229_M352_D0.5740.19
218_F222_L0.5740.19
172_E178_I0.5730.19
26_E135_L0.5720.19
403_K458_E0.5710.19
309_Q373_F0.5710.19
277_D422_S0.5710.19
337_P384_K0.5700.18
334_V347_E0.5700.18
263_I309_Q0.5690.18
65_E195_K0.5690.18
42_I51_V0.5690.18
266_S309_Q0.5680.18
254_Q312_A0.5670.18
85_N88_E0.5660.18
255_P321_R0.5640.18
104_I354_V0.5640.18
333_K406_W0.5630.18
44_L351_L0.5610.18
69_K92_N0.5610.18
51_V55_V0.5600.18
178_I275_I0.5600.18
213_S237_I0.5600.18
170_I373_F0.5600.18
337_P372_I0.5600.18
123_I126_V0.5590.18
131_S178_I0.5590.18
35_E53_T0.5590.18
73_S172_E0.5550.18
27_L55_V0.5540.18
247_Y266_S0.5530.18
226_K338_D0.5530.18
237_I243_E0.5520.18
136_A269_V0.5520.18
168_E195_K0.5510.18
330_T351_L0.5510.18
337_P436_I0.5500.18
123_I127_K0.5500.18
67_G329_F0.5500.18
322_P453_Y0.5490.18
309_Q437_E0.5490.18
102_F231_S0.5480.18
94_I102_F0.5470.18
254_Q383_F0.5470.18
179_G279_D0.5460.18
283_N358_N0.5450.18
223_E227_D0.5450.18
117_I431_A0.5440.18
291_F294_Y0.5440.18
388_F399_G0.5430.18
214_K369_L0.5430.18
388_F419_I0.5430.18
334_V444_L0.5420.17
221_F454_S0.5420.17
137_L140_L0.5410.17
11_K297_K0.5410.17
249_F364_E0.5400.17
279_D283_N0.5400.17
308_S390_F0.5380.17
75_L362_F0.5370.17
254_Q430_G0.5350.17
143_A160_K0.5350.17
165_V187_L0.5350.17
135_L189_I0.5340.17
151_L167_V0.5330.17
335_F349_H0.5320.17
80_L321_R0.5310.17
132_S286_E0.5300.17
128_T331_L0.5300.17
46_R51_V0.5300.17
401_L453_Y0.5290.17
224_K305_M0.5280.17
139_N432_W0.5280.17
194_K314_T0.5270.17
267_F347_E0.5260.17
137_L273_G0.5250.17
243_E248_N0.5250.17
254_Q369_L0.5250.17
70_R232_L0.5240.17
283_N301_T0.5230.17
188_I192_E0.5230.17
22_T82_N0.5220.17
237_I352_D0.5220.17
398_Y401_L0.5210.17
384_K449_I0.5210.17
266_S372_I0.5200.17
370_K428_T0.5190.17
237_I420_L0.5180.17
165_V351_L0.5180.17
184_N260_A0.5180.17
72_E146_N0.5180.17
226_K354_V0.5170.17
50_Y223_E0.5170.17
251_L420_L0.5170.17
266_S332_G0.5170.17
254_Q304_L0.5160.17
376_L397_A0.5150.17
83_T193_W0.5150.17
337_P398_Y0.5130.16
409_M446_I0.5120.16
112_K151_L0.5110.16
66_E104_I0.5110.16
198_L237_I0.5100.16
139_N144_D0.5090.16
21_K168_E0.5090.16
40_S61_Y0.5080.16
73_S124_P0.5080.16
247_Y423_V0.5070.16
16_L22_T0.5060.16
159_E223_E0.5050.16
18_D195_K0.5050.16
44_L411_G0.5030.16
218_F309_Q0.5020.16
264_H395_V0.5010.16
244_M390_F0.5010.16
243_E264_H0.5000.16
380_E446_I0.5000.16
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3l4gA 2 0.9915 100 0.389 Contact Map
1b7yA 2 0.5299 100 0.67 Contact Map
2odrC 4 0.5598 100 0.675 Contact Map
2du7A 7 0.6154 100 0.685 Contact Map
2odrD 4 0.5598 100 0.685 Contact Map
2odrA 4 0.5598 100 0.685 Contact Map
2odrB 3 0.5598 100 0.686 Contact Map
2du3A 4 0.6175 100 0.69 Contact Map
4p72C 2 0.5043 100 0.703 Contact Map
3pcoA 2 0.6581 100 0.712 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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