Y103 - SULSO - GREMLIN Contacts

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Y103 - Protein SSO0103

UniProt:	P95958	Sequences: MVSIVNIFMDYWFAEIITIGNEVLSGKTVNTNASHIGRRLTSLGFTVRRITAVMDEVDEIASAFREAIDRKPRVIVSSGGLGPTWDDKTAEGLAKALGVNLELNKTAFDMILEKYMTRKIPITEERKKMAYMPYGAIPVENNEGIAPGIYVYHNNIDILATPGVPREMENVLENFINKMLRNRSNLKYLEDFIYVENVMESSLAPYVKELVKKYDIYIKTHPKSYEMSHPILEIQIAGSGKQEEEIKVKIEKVKFELLDAIKKLNGIIRNSL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	272 (242)
Sequences:	2997 (2164)
Seq/√Len:	139.1

ECOLI - CINAL
BACSU - CINA
HALSA - Q9HRC4
SULSO - Y103

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
57_V	95_K	3.866	1.00
100_N	134_Y	3.258	1.00
99_V	135_G	3.042	1.00
102_E	134_Y	2.979	1.00
103_L	131_Y	2.962	1.00
57_V	92_G	2.850	1.00
29_V	34_S	2.817	1.00
39_R	42_S	2.723	1.00
14_A	45_F	2.675	1.00
61_A	95_K	2.508	1.00
209_E	260_A	2.439	1.00
39_R	173_E	2.439	1.00
15_E	70_R	2.433	1.00
78_S	168_M	2.343	1.00
243_E	247_K	2.294	1.00
76_V	161_T	2.276	1.00
108_F	127_K	2.203	1.00
108_F	112_L	2.190	1.00
240_G	246_I	2.158	1.00
93_L	160_A	2.058	1.00
105_K	109_D	2.012	1.00
30_N	33_A	2.011	1.00
103_L	108_F	1.975	1.00
112_L	116_M	1.961	1.00
35_H	173_E	1.928	1.00
91_E	128_K	1.905	1.00
15_E	67_A	1.874	1.00
109_D	112_L	1.859	1.00
150_Y	179_M	1.857	1.00
18_T	52_A	1.849	1.00
142_N	175_F	1.790	1.00
36_I	172_L	1.737	1.00
103_L	127_K	1.714	1.00
109_D	113_E	1.661	1.00
104_N	107_A	1.640	1.00
35_H	172_L	1.614	1.00
56_E	59_E	1.603	1.00
99_V	134_Y	1.596	1.00
191_D	254_K	1.568	1.00
132_M	138_P	1.528	1.00
30_N	50_I	1.476	1.00
64_F	96_A	1.475	1.00
124_E	127_K	1.474	1.00
148_G	171_V	1.461	1.00
49_R	66_E	1.455	1.00
21_N	217_Y	1.450	1.00
17_I	67_A	1.434	1.00
57_V	61_A	1.434	1.00
142_N	174_N	1.432	1.00
68_I	158_I	1.430	1.00
15_E	71_K	1.420	1.00
62_S	66_E	1.411	1.00
65_R	68_I	1.403	1.00
150_Y	159_L	1.399	1.00
256_E	259_D	1.394	1.00
251_E	255_F	1.390	1.00
191_D	251_E	1.373	1.00
242_Q	245_E	1.360	1.00
58_D	62_S	1.345	1.00
61_A	92_G	1.340	1.00
73_R	156_I	1.339	1.00
43_L	177_N	1.335	1.00
61_A	65_R	1.328	1.00
37_G	50_I	1.320	1.00
64_F	75_I	1.320	1.00
33_A	50_I	1.319	1.00
152_Y	183_R	1.314	1.00
17_I	63_A	1.302	1.00
60_I	92_G	1.295	1.00
122_I	127_K	1.286	1.00
15_E	51_T	1.285	1.00
51_T	66_E	1.274	1.00
215_D	249_K	1.251	1.00
68_I	96_A	1.249	1.00
77_S	160_A	1.249	1.00
40_L	76_V	1.247	1.00
115_Y	126_R	1.242	1.00
149_I	160_A	1.242	1.00
111_I	130_A	1.239	1.00
247_K	251_E	1.236	1.00
65_R	69_D	1.219	1.00
159_L	176_I	1.219	1.00
139_V	150_Y	1.209	1.00
68_I	156_I	1.206	1.00
112_L	127_K	1.205	1.00
64_F	77_S	1.203	1.00
53_V	63_A	1.193	1.00
213_K	252_K	1.192	1.00
254_K	258_L	1.190	1.00
62_S	70_R	1.182	1.00
204_A	208_K	1.170	0.99
137_I	179_M	1.160	0.99
193_I	254_K	1.157	0.99
129_M	145_I	1.152	0.99
37_G	47_V	1.148	0.99
65_R	96_A	1.141	0.99
245_E	248_V	1.138	0.99
126_R	129_M	1.136	0.99
97_L	158_I	1.135	0.99
164_V	170_N	1.125	0.99
90_A	149_I	1.121	0.99
15_E	49_R	1.121	0.99
194_Y	231_I	1.119	0.99
191_D	250_I	1.115	0.99
39_R	177_N	1.113	0.99
35_H	169_E	1.112	0.99
16_I	78_S	1.111	0.99
139_V	176_I	1.110	0.99
133_P	149_I	1.107	0.99
54_M	59_E	1.107	0.99
110_M	140_E	1.095	0.99
99_V	153_H	1.092	0.99
13_F	46_T	1.085	0.99
114_K	118_R	1.084	0.99
220_T	234_I	1.078	0.99
28_T	34_S	1.075	0.99
202_S	206_Y	1.070	0.99
45_F	180_L	1.066	0.99
172_L	176_I	1.053	0.99
236_I	254_K	1.053	0.99
209_E	213_K	1.046	0.99
215_D	245_E	1.036	0.99
18_T	23_V	1.035	0.99
236_I	250_I	1.035	0.99
99_V	151_V	1.034	0.99
250_I	254_K	1.031	0.99
198_V	203_L	1.022	0.99
85_W	208_K	1.022	0.99
51_T	63_A	1.012	0.99
168_M	172_L	1.010	0.98
77_S	93_L	1.000	0.98
170_N	174_N	0.999	0.98
238_G	246_I	0.998	0.98
74_V	159_L	0.996	0.98
139_V	175_F	0.996	0.98
199_M	202_S	0.994	0.98
64_F	93_L	0.993	0.98
113_E	117_T	0.992	0.98
14_A	74_V	0.991	0.98
36_I	168_M	0.989	0.98
191_D	247_K	0.986	0.98
101_L	131_Y	0.985	0.98
190_E	237_A	0.978	0.98
104_N	132_M	0.976	0.98
57_V	88_K	0.974	0.98
126_R	201_S	0.973	0.98
93_L	158_I	0.970	0.98
248_V	252_K	0.965	0.98
175_F	179_M	0.962	0.98
46_T	239_S	0.959	0.98
253_V	256_E	0.955	0.98
61_A	96_A	0.955	0.98
223_K	233_E	0.954	0.98
172_L	177_N	0.952	0.98
143_E	170_N	0.947	0.98
244_E	248_V	0.947	0.98
251_E	254_K	0.942	0.98
115_Y	122_I	0.941	0.98
152_Y	155_N	0.941	0.98
39_R	172_L	0.938	0.98
142_N	170_N	0.934	0.97
67_A	71_K	0.932	0.97
252_K	256_E	0.921	0.97
94_A	101_L	0.904	0.97
75_I	158_I	0.902	0.97
34_S	38_R	0.898	0.97
51_T	70_R	0.897	0.97
108_F	122_I	0.897	0.97
203_L	234_I	0.892	0.97
105_K	108_F	0.883	0.96
53_V	59_E	0.880	0.96
18_T	33_A	0.877	0.96
207_V	218_I	0.876	0.96
138_P	147_P	0.870	0.96
210_L	257_L	0.863	0.96
161_T	176_I	0.862	0.96
19_I	221_H	0.858	0.96
19_I	77_S	0.858	0.96
16_I	36_I	0.858	0.96
75_I	156_I	0.856	0.96
137_I	152_Y	0.852	0.96
97_L	151_V	0.851	0.96
214_Y	252_K	0.846	0.96
19_I	60_I	0.844	0.95
16_I	47_V	0.842	0.95
161_T	172_L	0.840	0.95
140_E	175_F	0.831	0.95
105_K	112_L	0.831	0.95
195_V	203_L	0.829	0.95
194_Y	233_E	0.827	0.95
113_E	116_M	0.826	0.95
106_T	109_D	0.823	0.95
160_A	164_V	0.820	0.95
214_Y	218_I	0.818	0.95
150_Y	157_D	0.815	0.95
195_V	198_V	0.808	0.94
144_G	167_E	0.807	0.94
60_I	64_F	0.806	0.94
85_W	220_T	0.802	0.94
62_S	69_D	0.801	0.94
204_A	220_T	0.792	0.94
157_D	183_R	0.790	0.94
141_N	146_A	0.789	0.94
189_L	243_E	0.785	0.93
17_I	75_I	0.785	0.93
48_R	59_E	0.780	0.93
67_A	75_I	0.779	0.93
132_M	149_I	0.775	0.93
152_Y	157_D	0.774	0.93
215_D	238_G	0.772	0.93
63_A	67_A	0.771	0.93
99_V	133_P	0.770	0.93
33_A	37_G	0.768	0.93
90_A	221_H	0.761	0.92
255_F	258_L	0.759	0.92
56_E	217_Y	0.753	0.92
159_L	179_M	0.746	0.91
97_L	133_P	0.743	0.91
123_T	205_P	0.743	0.91
57_V	91_E	0.735	0.91
256_E	260_A	0.734	0.91
244_E	247_K	0.731	0.91
107_A	132_M	0.727	0.90
104_N	138_P	0.726	0.90
143_E	166_R	0.725	0.90
152_Y	156_I	0.725	0.90
24_L	221_H	0.722	0.90
141_N	144_G	0.719	0.90
161_T	168_M	0.719	0.90
182_N	248_V	0.715	0.90
21_N	222_P	0.715	0.90
35_H	168_M	0.711	0.89
34_S	37_G	0.708	0.89
192_F	233_E	0.707	0.89
199_M	232_L	0.702	0.89
30_N	52_A	0.702	0.89
146_A	167_E	0.701	0.89
132_M	147_P	0.698	0.89
109_D	116_M	0.698	0.89
18_T	30_N	0.694	0.88
175_F	178_K	0.693	0.88
27_K	221_H	0.693	0.88
58_D	95_K	0.691	0.88
211_V	218_I	0.685	0.88
106_T	140_E	0.684	0.88
63_A	66_E	0.684	0.88
90_A	160_A	0.683	0.87
51_T	67_A	0.683	0.87
186_L	241_K	0.681	0.87
161_T	171_V	0.680	0.87
139_V	148_G	0.674	0.87
209_E	212_K	0.668	0.86
110_M	113_E	0.665	0.86
255_F	259_D	0.665	0.86
62_S	65_R	0.662	0.86
249_K	253_V	0.660	0.86
107_A	110_M	0.657	0.85
43_L	172_L	0.656	0.85
236_I	253_V	0.653	0.85
211_V	214_Y	0.642	0.84
81_L	167_E	0.642	0.84
132_M	136_A	0.641	0.84
48_R	56_E	0.640	0.84
13_F	48_R	0.639	0.84
136_A	151_V	0.638	0.84
47_V	50_I	0.636	0.83
19_I	222_P	0.635	0.83
186_L	239_S	0.632	0.83
252_K	255_F	0.632	0.83
21_N	54_M	0.628	0.83
248_V	251_E	0.628	0.83
110_M	114_K	0.627	0.83
107_A	138_P	0.624	0.82
107_A	141_N	0.624	0.82
85_W	211_V	0.623	0.82
139_V	171_V	0.623	0.82
210_L	253_V	0.619	0.82
141_N	148_G	0.619	0.82
187_K	240_G	0.618	0.82
103_L	130_A	0.617	0.82
55_D	84_T	0.613	0.81
68_I	154_N	0.612	0.81
17_I	77_S	0.612	0.81
85_W	212_K	0.610	0.81
189_L	246_I	0.608	0.81
85_W	204_A	0.608	0.81
164_V	171_V	0.608	0.81
207_V	211_V	0.602	0.80
93_L	151_V	0.599	0.80
67_A	70_R	0.599	0.80
27_K	237_A	0.599	0.80
189_L	250_I	0.597	0.79
14_A	40_L	0.595	0.79
35_H	39_R	0.595	0.79
157_D	179_M	0.595	0.79
116_M	121_P	0.588	0.78
34_S	169_E	0.587	0.78
128_K	131_Y	0.586	0.78
125_E	205_P	0.586	0.78
32_N	168_M	0.585	0.78
92_G	96_A	0.585	0.78
164_V	222_P	0.581	0.78
45_F	74_V	0.580	0.77
23_V	28_T	0.580	0.77
205_P	208_K	0.580	0.77
123_T	127_K	0.578	0.77
49_R	63_A	0.578	0.77
31_T	169_E	0.575	0.77
38_R	42_S	0.574	0.77
83_P	201_S	0.572	0.77
58_D	61_A	0.572	0.77
24_L	52_A	0.570	0.76
139_V	179_M	0.565	0.76
153_H	158_I	0.565	0.76
107_A	111_I	0.564	0.76
32_N	164_V	0.560	0.75
61_A	64_F	0.559	0.75
238_G	249_K	0.559	0.75
94_A	100_N	0.558	0.75
110_M	145_I	0.556	0.75
59_E	63_A	0.556	0.75
18_T	168_M	0.555	0.74
88_K	92_G	0.553	0.74
53_V	67_A	0.553	0.74
21_N	220_T	0.550	0.74
40_L	45_F	0.548	0.73
90_A	219_K	0.548	0.73
29_V	38_R	0.547	0.73
190_E	217_Y	0.546	0.73
213_K	256_E	0.545	0.73
145_I	167_E	0.541	0.73
249_K	252_K	0.541	0.73
99_V	152_Y	0.540	0.72
19_I	89_T	0.539	0.72
133_P	151_V	0.537	0.72
38_R	169_E	0.537	0.72
55_D	86_D	0.536	0.72
25_S	235_Q	0.535	0.72
185_N	241_K	0.531	0.71
203_L	206_Y	0.527	0.71
16_I	33_A	0.526	0.70
244_E	255_F	0.522	0.70
129_M	146_A	0.521	0.70
210_L	218_I	0.521	0.70
97_L	153_H	0.520	0.70
146_A	171_V	0.520	0.70
79_G	82_G	0.518	0.69
92_G	95_K	0.518	0.69
55_D	163_G	0.517	0.69
49_R	59_E	0.516	0.69
133_P	145_I	0.515	0.69
204_A	222_P	0.514	0.69
21_N	172_L	0.511	0.68
59_E	190_E	0.510	0.68
137_I	150_Y	0.510	0.68
107_A	171_V	0.509	0.68
123_T	202_S	0.506	0.67
19_I	164_V	0.505	0.67
189_L	247_K	0.504	0.67
64_F	158_I	0.504	0.67
195_V	231_I	0.504	0.67
144_G	162_P	0.501	0.67
161_T	164_V	0.500	0.67

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4uuwA	2	0.9081	100	0.266	Contact Map
3kbqA	2	0.625	100	0.46	Contact Map
1mkzA	2	0.6213	100	0.607	Contact Map
1y5eA	4	0.5809	100	0.61	Contact Map
2is8A	3	0.5846	100	0.614	Contact Map
3iwtA	4	0.5699	100	0.615	Contact Map
2g2cA	1	0.5478	100	0.617	Contact Map
4lhbA	4	0.5809	100	0.624	Contact Map
3pzyA	3	0.5478	100	0.629	Contact Map
3rfqA	5	0.5735	100	0.63	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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