GREMLIN Database
TREZ - Malto-oligosyltrehalose trehalohydrolase
UniProt: P95867 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 561 (472)
Sequences: 10758 (7037)
Seq/√Len: 323.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
129_N183_L4.5901.00
122_E175_K4.4391.00
179_E182_K4.1761.00
122_E172_G3.7251.00
133_E481_K3.6421.00
233_R271_D3.3741.00
116_T127_K3.3401.00
174_R246_E3.1021.00
132_K183_L3.0701.00
139_I180_A3.0161.00
404_A480_Y2.9601.00
409_L509_V2.9471.00
169_G172_G2.9331.00
179_E183_L2.8931.00
36_M46_I2.7941.00
485_K489_E2.7471.00
423_G476_I2.7271.00
181_H250_D2.6441.00
173_F177_V2.5591.00
377_Q420_E2.5121.00
128_L183_L2.4981.00
277_H306_D2.4461.00
234_K238_E2.3211.00
122_E125_I2.2931.00
92_W248_H2.2771.00
242_Y247_F2.1911.00
175_K179_E2.1701.00
125_I175_K2.1551.00
196_V210_F2.1061.00
124_V176_L2.0951.00
104_D494_C2.0881.00
141_I144_I2.0871.00
173_F249_V2.0691.00
244_I281_K2.0681.00
478_S481_K2.0511.00
501_E504_N2.0491.00
408_I484_I2.0431.00
171_S174_R2.0261.00
510_K513_G2.0251.00
180_A185_L2.0231.00
409_L516_A2.0121.00
125_I179_E2.0091.00
241_E275_V1.9921.00
98_V101_K1.9841.00
97_S101_K1.9841.00
106_V484_I1.9821.00
180_A187_V1.9791.00
105_L138_A1.9481.00
275_V278_K1.9061.00
193_Y239_N1.9051.00
209_Y212_E1.8971.00
273_A277_H1.8901.00
52_R55_D1.8791.00
146_Q162_A1.8591.00
141_I176_L1.8561.00
405_A479_F1.8551.00
131_L136_V1.8341.00
490_Y512_N1.8311.00
23_K35_E1.8191.00
482_S485_K1.8161.00
110_L115_F1.8161.00
23_K62_D1.7981.00
90_F178_N1.7811.00
25_K60_I1.7801.00
94_D248_H1.7801.00
12_V85_I1.7691.00
133_E477_L1.7631.00
11_G47_T1.7561.00
441_Q445_E1.7361.00
401_K479_F1.7231.00
490_Y514_C1.7221.00
172_G175_K1.7201.00
508_T517_V1.7101.00
510_K515_L1.6981.00
161_Y239_N1.6781.00
34_Y46_I1.6621.00
478_S482_S1.6541.00
124_V141_I1.6361.00
176_L180_A1.6311.00
33_I60_I1.6181.00
178_N182_K1.6161.00
12_V51_V1.6071.00
15_N45_T1.5971.00
112_I124_V1.5951.00
24_V61_L1.5931.00
241_E245_N1.5891.00
240_V272_I1.5721.00
76_P80_H1.5711.00
173_F176_L1.5641.00
146_Q165_N1.5631.00
14_F59_Y1.5611.00
126_K179_E1.5421.00
93_D178_N1.5391.00
392_I395_V1.5311.00
161_Y238_E1.5271.00
477_L481_K1.5191.00
217_P222_F1.5191.00
131_L185_L1.5151.00
144_I173_F1.5131.00
474_D478_S1.5111.00
122_E126_K1.5071.00
60_I66_E1.5041.00
25_K62_D1.4931.00
131_L139_I1.4861.00
25_K35_E1.4851.00
56_R76_P1.4851.00
242_Y246_E1.4781.00
51_V55_D1.4541.00
105_L282_I1.4471.00
90_F93_D1.4361.00
226_D229_S1.4311.00
130_Y477_L1.4251.00
163_V173_F1.4241.00
276_V281_K1.4191.00
110_L124_V1.4161.00
72_S83_S1.4131.00
56_R84_E1.4121.00
505_Y521_S1.4091.00
95_E248_H1.4061.00
268_I272_I1.4051.00
25_K33_I1.4031.00
215_K222_F1.3981.00
218_W221_T1.3901.00
22_R62_D1.3891.00
492_L514_C1.3881.00
422_Y443_V1.3851.00
22_R64_N1.3841.00
245_N279_Y1.3741.00
215_K218_W1.3661.00
16_L61_L1.3591.00
402_I518_Y1.3581.00
401_K476_I1.3451.00
188_I284_I1.3441.00
16_L24_V1.3391.00
207_G212_E1.3331.00
110_L131_L1.3251.00
13_T47_T1.3161.00
218_W224_F1.3061.00
275_V279_Y1.3051.00
13_T45_T1.2831.00
241_E279_Y1.2781.00
393_K425_E1.2741.00
138_A186_A1.2691.00
74_Y86_I1.2641.00
231_E234_K1.2631.00
271_D275_V1.2621.00
230_D234_K1.2581.00
191_V240_V1.2571.00
321_L403_A1.2501.00
90_F175_K1.2491.00
340_K343_K1.2481.00
128_L180_A1.2441.00
219_G222_F1.2321.00
110_L139_I1.2261.00
220_L223_N1.2261.00
105_L137_T1.2231.00
15_N43_Y1.2161.00
68_P75_Q1.2131.00
138_A188_I1.2091.00
237_L275_V1.2071.00
31_R60_I1.2031.00
120_T123_G1.1971.00
27_K60_I1.1931.00
90_F248_H1.1921.00
217_P221_T1.1911.00
34_Y48_L1.1891.00
75_Q159_Y1.1861.00
402_I520_F1.1821.00
218_W222_F1.1791.00
129_N133_E1.1791.00
128_L179_E1.1751.00
429_Y441_Q1.1751.00
188_I251_G1.1661.00
267_H270_E1.1631.00
173_F189_L1.1601.00
441_Q444_R1.1561.00
509_V516_A1.1531.00
127_K130_Y1.1521.00
112_I141_I1.1491.00
272_I283_V1.1451.00
404_A416_I1.1441.00
145_A239_N1.1431.00
532_T535_L1.1411.00
27_K33_I1.1411.00
209_Y213_K1.1381.00
121_F163_V1.1361.00
72_S75_Q1.1351.00
204_V208_P1.1341.00
244_I276_V1.1341.00
404_A483_L1.1291.00
146_Q164_Q1.1261.00
416_I480_Y1.1211.00
339_V342_Y1.1151.00
93_D182_K1.1091.00
131_L135_G1.1091.00
214_Y217_P1.1061.00
375_Y378_N1.1061.00
274_D298_K1.1031.00
481_K485_K1.1021.00
447_R451_N1.0961.00
313_F410_S1.0941.00
215_K220_L1.0851.00
271_D274_D1.0841.00
116_T126_K1.0781.00
401_K475_D1.0781.00
74_Y84_E1.0751.00
210_F213_K1.0731.00
533_L536_S1.0711.00
137_T488_K1.0711.00
113_G117_S1.0661.00
416_I420_E1.0661.00
134_L477_L1.0641.00
438_K441_Q1.0611.00
107_I374_V1.0531.00
276_V279_Y1.0521.00
339_V343_K1.0511.00
317_I403_A1.0491.00
145_A157_G1.0481.00
400_Y423_G1.0471.00
220_L224_F1.0431.00
207_G210_F1.0401.00
115_F124_V1.0381.00
113_G167_Y1.0361.00
418_M443_V1.0361.00
121_F172_G1.0361.00
444_R447_R1.0351.00
234_K275_V1.0331.00
161_Y242_Y1.0331.00
270_E274_D1.0311.00
237_L272_I1.0301.00
154_G167_Y1.0291.00
217_P220_L1.0281.00
338_I344_D1.0271.00
273_A283_V1.0221.00
404_A420_E1.0211.00
51_V85_I1.0201.00
189_L249_V1.0191.00
219_G224_F1.0191.00
295_V298_K1.0171.00
26_L46_I1.0151.00
332_F335_I1.0131.00
161_Y235_F1.0031.00
198_P214_Y1.0021.00
527_M530_S1.0021.00
296_N299_E1.0011.00
319_A322_T1.0011.00
173_F247_F1.0001.00
406_L518_Y0.9991.00
163_V243_W0.9991.00
217_P224_F0.9961.00
12_V52_R0.9921.00
338_I342_Y0.9901.00
215_K224_F0.9871.00
204_V207_G0.9851.00
240_V254_L0.9831.00
376_I403_A0.9821.00
442_G446_G0.9781.00
342_Y345_V0.9741.00
132_K185_L0.9731.00
215_K223_N0.9731.00
338_I341_S0.9731.00
107_I188_I0.9721.00
528_K532_T0.9701.00
484_I488_K0.9701.00
440_I443_V0.9661.00
237_L241_E0.9651.00
126_K129_N0.9651.00
28_I34_Y0.9641.00
474_D477_L0.9561.00
341_S344_D0.9541.00
215_K221_T0.9531.00
309_W314_H0.9521.00
214_Y219_G0.9511.00
107_I138_A0.9501.00
324_E327_G0.9491.00
67_V70_P0.9421.00
106_V408_I0.9411.00
175_K178_N0.9401.00
448_R477_L0.9371.00
319_A395_V0.9371.00
55_D85_I0.9371.00
408_I416_I0.9361.00
528_K531_G0.9341.00
239_N242_Y0.9341.00
339_V344_D0.9321.00
78_G150_K0.9161.00
293_R296_N0.9141.00
218_W223_N0.9141.00
119_G123_G0.9121.00
69_D72_S0.9101.00
106_V488_K0.9101.00
170_P247_F0.9081.00
274_D278_K0.9041.00
140_E309_W0.9021.00
486_I490_Y0.9021.00
393_K396_D0.9001.00
445_E449_R0.8981.00
129_N132_K0.8981.00
237_L271_D0.8971.00
221_T224_F0.8941.00
269_L272_I0.8931.00
391_L395_V0.8921.00
160_L235_F0.8921.00
58_K66_E0.8921.00
39_D45_T0.8881.00
340_K344_D0.8861.00
342_Y346_F0.8851.00
267_H271_D0.8851.00
75_Q162_A0.8821.00
102_R282_I0.8821.00
270_E304_N0.8741.00
240_V252_F0.8721.00
208_P211_S0.8711.00
144_I189_L0.8691.00
336_G339_V0.8641.00
315_H319_A0.8641.00
121_F168_G0.8631.00
112_I121_F0.8611.00
311_D314_H0.8601.00
163_V242_Y0.8591.00
177_V187_V0.8581.00
219_G223_N0.8581.00
216_T220_L0.8551.00
446_G449_R0.8541.00
171_S175_K0.8501.00
125_I176_L0.8501.00
37_E45_T0.8491.00
114_T152_D0.8441.00
317_I406_L0.8441.00
208_P213_K0.8381.00
193_Y236_I0.8371.00
68_P72_S0.8361.00
157_G160_L0.8351.00
517_V527_M0.8331.00
255_D286_E0.8321.00
405_A483_L0.8311.00
422_Y477_L0.8301.00
28_I31_R0.8301.00
53_V87_S0.8281.00
479_F483_L0.8261.00
492_L512_N0.8251.00
67_V71_A0.8241.00
448_R451_N0.8231.00
136_V185_L0.8211.00
90_F94_D0.8201.00
233_R267_H0.8181.00
137_T185_L0.8171.00
404_A476_I0.8151.00
293_R297_P0.8131.00
100_V138_A0.8131.00
110_L418_M0.8121.00
292_P295_V0.8121.00
134_L484_I0.8121.00
17_W231_E0.8101.00
214_Y222_F0.8091.00
399_S402_I0.8051.00
28_I55_D0.8041.00
216_T221_T0.8041.00
318_H322_T0.8031.00
89_D171_S0.8031.00
115_F130_Y0.8031.00
400_Y476_I0.8021.00
530_S536_S0.8001.00
272_I276_V0.8001.00
294_V297_P0.7991.00
66_E69_D0.7981.00
240_V276_V0.7981.00
97_S100_V0.7971.00
493_A514_C0.7971.00
118_E123_G0.7971.00
68_P71_A0.7971.00
48_L51_V0.7961.00
125_I172_G0.7951.00
205_K208_P0.7951.00
477_L480_Y0.7941.00
266_K270_E0.7921.00
119_G127_K0.7911.00
337_D340_K0.7901.00
209_Y235_F0.7891.00
124_V180_A0.7881.00
170_P246_E0.7811.00
370_C400_Y0.7811.00
180_A183_L0.7771.00
66_E70_P0.7751.00
195_H255_D0.7751.00
145_A249_V0.7721.00
499_S510_K0.7711.00
19_P42_G0.7711.00
104_D495_N0.7711.00
145_A189_L0.7671.00
162_A170_P0.7651.00
377_Q403_A0.7651.00
27_K58_K0.7641.00
508_T511_G0.7610.99
309_W313_F0.7600.99
276_V283_V0.7600.99
409_L518_Y0.7600.99
313_F317_I0.7590.99
405_A409_L0.7510.99
527_M532_T0.7510.99
497_K500_V0.7480.99
215_K219_G0.7450.99
100_V184_G0.7440.99
241_E276_V0.7440.99
528_K533_L0.7430.99
106_V136_V0.7420.99
126_K175_K0.7390.99
195_H379_H0.7380.99
297_P346_F0.7380.99
346_F369_G0.7360.99
91_E94_D0.7330.99
122_E169_G0.7320.99
214_Y218_W0.7320.99
40_D45_T0.7320.99
302_G305_I0.7310.99
507_L511_G0.7310.99
216_T222_F0.7300.99
303_Y306_D0.7290.99
130_Y133_E0.7280.99
284_I415_M0.7210.99
132_K184_G0.7210.99
208_P212_E0.7200.99
68_P83_S0.7190.99
74_Y77_E0.7190.99
406_L520_F0.7180.99
18_A21_Q0.7170.99
216_T223_N0.7170.99
92_W95_E0.7140.99
286_E379_H0.7110.99
310_V314_H0.7100.99
88_P92_W0.7090.99
505_Y511_G0.7080.99
476_I479_F0.7080.99
491_G495_N0.7070.99
521_S525_I0.7060.99
159_Y162_A0.7060.99
378_N392_I0.7060.99
532_T536_S0.7060.99
302_G306_D0.7030.99
440_I444_R0.7030.99
14_F85_I0.7030.99
444_R448_R0.7030.99
343_K346_F0.7030.99
444_R449_R0.7020.99
66_E71_A0.7020.99
527_M531_G0.6990.99
53_V89_D0.6990.99
255_D380_D0.6950.99
109_E142_M0.6930.99
253_R286_E0.6920.99
282_I307_A0.6920.99
339_V346_F0.6910.99
405_A518_Y0.6900.99
146_Q158_V0.6880.99
373_V414_P0.6880.99
389_E399_S0.6870.99
525_I528_K0.6860.99
338_I343_K0.6850.99
27_K34_Y0.6850.99
121_F144_I0.6840.99
22_R63_D0.6840.99
36_M59_Y0.6840.99
323_G327_G0.6830.99
22_R65_S0.6830.99
400_Y404_A0.6810.99
70_P74_Y0.6790.99
23_K60_I0.6780.99
129_N179_E0.6770.99
319_A324_E0.6740.99
341_S345_V0.6740.99
508_T515_L0.6710.99
217_P223_N0.6700.99
443_V448_R0.6700.99
204_V210_F0.6700.99
177_V249_V0.6690.99
339_V345_V0.6690.99
531_G536_S0.6680.99
67_V72_S0.6680.99
71_A74_Y0.6680.99
90_F174_R0.6650.99
393_K397_K0.6640.99
207_G211_S0.6640.99
319_A323_G0.6630.99
73_R242_Y0.6630.99
138_A185_L0.6620.99
37_E46_I0.6610.99
227_A236_I0.6600.99
108_Y421_E0.6600.99
205_K210_F0.6600.99
104_D488_K0.6590.99
69_D74_Y0.6570.99
430_Y441_Q0.6570.99
269_L276_V0.6550.98
531_G535_L0.6550.98
529_Y532_T0.6540.98
107_I373_V0.6540.98
244_I252_F0.6540.98
320_F520_F0.6530.98
409_L486_I0.6530.98
138_A282_I0.6530.98
340_K346_F0.6530.98
261_I266_K0.6530.98
528_K536_S0.6510.98
27_K31_R0.6510.98
178_N248_H0.6500.98
216_T219_G0.6500.98
53_V86_I0.6490.98
414_P487_R0.6490.98
76_P84_E0.6470.98
156_D197_G0.6470.98
235_F239_N0.6470.98
244_I279_Y0.6460.98
111_H142_M0.6460.98
338_I346_F0.6450.98
337_D343_K0.6450.98
150_K164_Q0.6450.98
530_S535_L0.6440.98
207_G213_K0.6440.98
73_R246_E0.6440.98
58_K82_Y0.6430.98
216_T224_F0.6420.98
398_E401_K0.6420.98
54_G84_E0.6420.98
230_D233_R0.6420.98
119_G122_E0.6410.98
530_S533_L0.6400.98
88_P91_E0.6390.98
214_Y221_T0.6380.98
124_V139_I0.6340.98
121_F173_F0.6330.98
309_W415_M0.6320.98
147_F157_G0.6300.98
174_R247_F0.6290.98
35_E38_R0.6290.98
394_L524_V0.6280.98
135_G485_K0.6270.98
253_R379_H0.6270.98
71_A209_Y0.6250.98
116_T119_G0.6240.98
130_Y422_Y0.6240.98
155_Y286_E0.6240.98
118_E442_G0.6230.98
292_P297_P0.6230.98
337_D344_D0.6220.98
195_H253_R0.6210.98
193_Y254_L0.6210.98
94_D178_N0.6210.98
70_P73_R0.6210.98
233_R237_L0.6200.98
506_W511_G0.6190.98
445_E448_R0.6190.98
76_P82_Y0.6190.98
255_D379_H0.6180.98
100_V104_D0.6150.98
439_L442_G0.6150.98
314_H318_H0.6150.98
442_G445_E0.6150.98
338_I345_V0.6140.98
403_A406_L0.6140.98
107_I415_M0.6140.98
425_E441_Q0.6130.98
62_D65_S0.6100.98
409_L483_L0.6100.98
274_D299_E0.6090.98
397_K451_N0.6080.98
506_W527_M0.6080.98
75_Q83_S0.6080.98
14_F26_L0.6070.98
206_L209_Y0.6070.98
444_R450_E0.6070.98
235_F238_E0.6060.97
91_E95_E0.6030.97
133_E478_S0.6020.97
93_D248_H0.6010.97
108_Y134_L0.6010.97
151_K430_Y0.6010.97
505_Y520_F0.6000.97
321_L520_F0.6000.97
181_H187_V0.5980.97
429_Y440_I0.5970.97
447_R450_E0.5960.97
132_K488_K0.5960.97
56_R82_Y0.5950.97
214_Y220_L0.5930.97
406_L410_S0.5910.97
341_S346_F0.5910.97
125_I129_N0.5910.97
89_D92_W0.5880.97
387_G396_D0.5850.97
258_H288_D0.5840.97
164_Q167_Y0.5840.97
310_V374_V0.5840.97
475_D478_S0.5830.97
430_Y438_K0.5810.97
427_P439_L0.5780.97
446_G450_E0.5780.97
115_F131_L0.5770.97
340_K345_V0.5750.97
337_D346_F0.5740.97
26_L48_L0.5730.97
123_G176_L0.5720.96
121_F176_L0.5720.96
443_V450_E0.5720.96
178_N181_H0.5720.96
146_Q166_S0.5710.96
529_Y536_S0.5710.96
16_L21_Q0.5710.96
426_N444_R0.5710.96
439_L445_E0.5690.96
527_M535_L0.5690.96
141_I173_F0.5690.96
501_E506_W0.5690.96
118_E126_K0.5690.96
94_D182_K0.5680.96
155_Y255_D0.5680.96
95_E178_N0.5670.96
385_R400_Y0.5670.96
123_G126_K0.5670.96
96_N99_K0.5660.96
136_V421_E0.5660.96
293_R298_K0.5650.96
88_P171_S0.5640.96
236_I268_I0.5620.96
174_R242_Y0.5580.96
491_G494_C0.5570.96
254_L283_V0.5560.96
370_C374_V0.5550.96
313_F316_A0.5550.96
195_H286_E0.5540.96
163_V170_P0.5530.96
26_L59_Y0.5530.96
295_V299_E0.5520.96
409_L493_A0.5500.96
438_K443_V0.5490.96
125_I183_L0.5490.96
97_S102_R0.5480.96
319_A341_S0.5470.95
500_V510_K0.5470.95
67_V74_Y0.5470.95
443_V447_R0.5460.95
18_A42_G0.5460.95
266_K304_N0.5440.95
71_A238_E0.5430.95
269_L273_A0.5430.95
174_R178_N0.5430.95
102_R105_L0.5430.95
294_V298_K0.5390.95
192_V286_E0.5370.95
10_D13_T0.5370.95
319_A325_R0.5370.95
476_I480_Y0.5360.95
321_L406_L0.5350.95
388_G391_L0.5340.95
192_V195_H0.5340.95
100_V103_E0.5340.95
507_L510_K0.5330.95
253_R380_D0.5330.95
39_D43_Y0.5330.95
501_E505_Y0.5320.95
29_L58_K0.5310.95
105_L188_I0.5300.95
112_I144_I0.5270.94
479_F482_S0.5270.94
223_N227_A0.5270.94
335_I338_I0.5270.94
48_L52_R0.5260.94
32_G60_I0.5240.94
382_V428_F0.5240.94
515_L531_G0.5230.94
193_Y240_V0.5230.94
66_E77_E0.5220.94
190_D255_D0.5210.94
238_E241_E0.5210.94
485_K488_K0.5210.94
200_G203_M0.5210.94
14_F48_L0.5200.94
274_D277_H0.5200.94
206_L210_F0.5200.94
273_A276_V0.5190.94
170_P242_Y0.5180.94
160_L224_F0.5180.94
188_I282_I0.5170.94
206_L212_E0.5150.94
420_E480_Y0.5130.94
288_D314_H0.5130.94
147_F167_Y0.5130.94
496_R499_S0.5130.94
396_D399_S0.5120.94
445_E451_N0.5120.94
131_L180_A0.5120.94
490_Y494_C0.5110.94
268_I275_V0.5110.94
481_K484_I0.5100.93
107_I413_I0.5090.93
135_G484_I0.5080.93
257_V269_L0.5070.93
47_T50_N0.5070.93
158_V197_G0.5070.93
336_G340_K0.5060.93
256_A259_A0.5060.93
186_A282_I0.5060.93
136_V416_I0.5050.93
506_W525_I0.5050.93
219_G259_A0.5040.93
192_V255_D0.5010.93
323_G341_S0.5010.93
35_E44_F0.5010.93
272_I275_V0.5000.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3vgfA 2 0.9804 100 0.244 Contact Map
2bhuA 1 0.9519 100 0.279 Contact Map
3wdhA 1 0.8913 100 0.282 Contact Map
1bf2A 1 0.9162 100 0.288 Contact Map
3m07A 1 0.9626 100 0.292 Contact Map
2wanA 1 0.9055 100 0.292 Contact Map
2e8yA 1 0.9127 100 0.293 Contact Map
2vr5A 5 0.9109 100 0.298 Contact Map
1ea9C 2 0.8681 100 0.304 Contact Map
1wzlA 2 0.8663 100 0.305 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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