GREMLIN Database
PNPH - Purine nucleoside phosphorylase
UniProt: P50389 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 236 (213)
Sequences: 5589 (3801)
Seq/√Len: 260.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
90_T203_V3.1081.00
190_S197_S3.0041.00
77_A195_W2.8411.00
15_E82_N2.7271.00
106_T151_V2.7111.00
35_N51_K2.5941.00
139_V151_V2.5701.00
107_G153_N2.5541.00
131_D196_K2.5461.00
183_C199_T2.4431.00
83_V196_K2.3771.00
107_G129_T2.2951.00
51_K56_T2.2141.00
105_V154_V2.1431.00
169_K173_S2.0761.00
155_F176_N2.0661.00
41_E46_L2.0601.00
94_V177_I2.0391.00
74_E193_K2.0051.00
139_V143_S1.9981.00
16_R83_V1.9751.00
136_N140_T1.8311.00
107_G127_A1.7951.00
37_K49_T1.7701.00
66_G185_T1.7591.00
98_N101_E1.6911.00
104_I135_T1.6401.00
90_T178_A1.6361.00
215_T218_E1.6281.00
103_I178_A1.6131.00
82_N195_W1.6101.00
23_P61_T1.6011.00
154_V178_A1.5941.00
135_T151_V1.5771.00
134_L198_A1.5741.00
132_F136_N1.5491.00
97_I150_Y1.5421.00
138_L198_A1.5411.00
166_E169_K1.5361.00
20_V26_A1.5351.00
180_E183_C1.4861.00
114_G118_Q1.4801.00
28_L224_M1.4771.00
45_F61_T1.4751.00
66_G156_S1.4741.00
73_L84_F1.4581.00
224_M228_K1.4531.00
152_G177_I1.4431.00
19_V62_H1.4381.00
40_N79_L1.4351.00
167_F170_K1.4251.00
110_Y188_T1.4091.00
27_R31_T1.4001.00
73_L189_L1.3801.00
142_F149_Y1.3751.00
19_V84_F1.3621.00
114_G119_Y1.3491.00
111_N157_S1.3491.00
169_K172_S1.3251.00
135_T198_A1.3221.00
115_L120_L1.3081.00
96_Y150_Y1.3071.00
154_V201_L1.2941.00
116_F119_Y1.2941.00
166_E170_K1.2931.00
104_I149_Y1.2841.00
34_Q51_K1.2761.00
167_F171_W1.2611.00
110_Y184_A1.2411.00
47_V60_A1.2401.00
178_A203_V1.2331.00
170_K173_S1.2261.00
52_Y231_L1.2231.00
16_R55_E1.2231.00
101_E150_Y1.2221.00
214_I218_E1.2201.00
221_K225_D1.2191.00
115_L119_Y1.2061.00
206_N209_K1.1991.00
53_N232_D1.1991.00
116_F120_L1.1991.00
108_A183_C1.1931.00
15_E56_T1.1901.00
49_T58_S1.1901.00
104_I139_V1.1861.00
136_N139_V1.1771.00
39_T46_L1.1691.00
108_A187_F1.1631.00
145_R228_K1.1481.00
49_T56_T1.1411.00
30_S48_Y1.1311.00
168_V172_S1.1301.00
141_S145_R1.1301.00
103_I177_I1.1291.00
140_T143_S1.1251.00
92_A203_V1.1221.00
141_S144_K1.1041.00
84_F190_S1.0851.00
28_L31_T1.0851.00
37_K56_T1.0851.00
145_R232_D1.0841.00
40_N49_T1.0781.00
17_V81_A1.0771.00
133_E196_K1.0771.00
187_F197_S1.0761.00
92_A97_I1.0691.00
26_A59_I1.0671.00
130_P135_T1.0631.00
168_V171_W1.0631.00
37_K40_N1.0621.00
60_A72_V1.0611.00
47_V58_S1.0581.00
135_T138_L1.0561.00
108_A199_T1.0501.00
39_T60_A1.0471.00
69_I186_L1.0421.00
150_Y177_I1.0341.00
137_K141_S1.0271.00
225_D228_K1.0231.00
92_A206_N1.0221.00
133_E136_N1.0121.00
77_A193_K1.0111.00
111_N159_A1.0111.00
21_G25_R1.0111.00
109_S127_A1.0111.00
77_A84_F1.0101.00
70_A189_L1.0041.00
29_L59_I1.0011.00
214_I217_E0.9801.00
133_E137_K0.9751.00
33_L50_G0.9731.00
28_L227_A0.9661.00
134_L138_L0.9651.00
217_E221_K0.9651.00
109_S155_F0.9591.00
114_G120_L0.9551.00
114_G117_Y0.9531.00
139_V149_Y0.9421.00
83_V134_L0.9381.00
165_E168_V0.9331.00
127_A153_N0.9301.00
140_T144_K0.9301.00
147_L229_A0.9281.00
86_R186_L0.9261.00
135_T139_V0.9201.00
104_I198_A0.9201.00
66_G112_Q0.9181.00
52_Y57_V0.9161.00
94_V97_I0.9131.00
132_F135_T0.9131.00
71_I189_L0.9111.00
100_G214_I0.9061.00
66_G158_D0.9001.00
20_V87_Y0.9001.00
51_K54_G0.8981.00
156_S183_C0.8971.00
105_V201_L0.8961.00
228_K232_D0.8961.00
98_N209_K0.8861.00
115_L118_Q0.8781.00
98_N206_N0.8741.00
93_L176_N0.8741.00
93_L179_V0.8691.00
144_K232_D0.8631.00
69_I185_T0.8631.00
130_P187_F0.8591.00
43_R75_E0.8480.99
69_I73_L0.8470.99
98_N211_G0.8400.99
23_P27_R0.8380.99
47_V75_E0.8340.99
165_E169_K0.8340.99
23_P30_S0.8290.99
24_G41_E0.8280.99
111_N155_F0.8270.99
70_A74_E0.8200.99
77_A81_A0.8190.99
136_N143_S0.8130.99
220_E224_M0.8040.99
102_Y229_A0.8000.99
131_D134_L0.7960.99
105_V178_A0.7960.99
116_F188_T0.7940.99
106_T153_N0.7920.99
100_G222_S0.7840.99
33_L36_P0.7770.99
42_N78_M0.7740.99
24_G27_R0.7740.99
105_V130_P0.7720.99
66_G184_A0.7640.99
99_L214_I0.7630.99
17_V76_L0.7540.99
223_V227_A0.7470.99
218_E222_S0.7460.99
26_A48_Y0.7410.99
60_A76_L0.7380.99
71_I74_E0.7330.98
89_T204_S0.7320.98
26_A61_T0.7250.98
221_K224_M0.7200.98
36_P48_Y0.7170.98
185_T189_L0.7140.98
34_Q54_G0.7060.98
215_T219_L0.7050.98
129_T132_F0.7050.98
105_V108_A0.7010.98
224_M227_A0.6990.98
95_P208_A0.6980.98
117_Y120_L0.6980.98
141_S232_D0.6970.98
103_I203_V0.6950.98
180_E199_T0.6940.98
128_S188_T0.6880.98
17_V58_S0.6850.98
29_L87_Y0.6840.98
32_L224_M0.6730.97
84_F195_W0.6730.97
129_T191_K0.6690.97
114_G123_N0.6680.97
142_F229_A0.6660.97
168_V173_S0.6650.97
89_T215_T0.6600.97
202_V218_E0.6590.97
71_I75_E0.6580.97
70_A185_T0.6580.97
30_S59_I0.6570.97
74_E189_L0.6560.97
93_L207_L0.6550.97
179_V205_D0.6520.97
166_E171_W0.6520.97
32_L228_K0.6500.97
104_I151_V0.6500.97
90_T201_L0.6460.97
77_A80_G0.6430.97
100_G218_E0.6370.96
214_I222_S0.6370.96
28_L32_L0.6320.96
109_S176_N0.6290.96
187_F198_A0.6290.96
15_E55_E0.6280.96
136_N151_V0.6260.96
138_L141_S0.6250.96
216_K219_L0.6240.96
220_E223_V0.6240.96
179_V207_L0.6140.96
40_N47_V0.6140.96
131_D137_K0.6140.96
31_T224_M0.6130.96
102_Y202_V0.6120.95
161_Y179_V0.6110.95
163_E166_E0.6100.95
62_H73_L0.6090.95
115_L189_L0.6070.95
163_E192_V0.6070.95
217_E220_E0.6070.95
22_D25_R0.6040.95
97_I103_I0.6030.95
86_R89_T0.6000.95
160_F181_M0.5970.95
183_C201_L0.5960.95
117_Y122_D0.5950.95
45_F72_V0.5920.95
204_S223_V0.5890.94
108_A154_V0.5890.94
22_D86_R0.5890.94
123_N126_V0.5830.94
109_S153_N0.5830.94
106_T132_F0.5790.94
94_V150_Y0.5780.94
218_E221_K0.5740.94
109_S126_V0.5710.93
27_R46_L0.5690.93
90_T103_I0.5670.93
134_L137_K0.5660.93
47_V79_L0.5620.93
144_K147_L0.5610.93
73_L186_L0.5610.93
45_F64_I0.5600.93
183_C187_F0.5590.93
20_V29_L0.5580.93
19_V73_L0.5550.93
153_N174_R0.5540.92
97_I203_V0.5540.92
41_E44_G0.5510.92
40_N44_G0.5510.92
105_V187_F0.5510.92
168_V207_L0.5490.92
147_L228_K0.5480.92
36_P39_T0.5460.92
21_G86_R0.5450.92
132_F139_V0.5430.92
76_L81_A0.5420.92
77_A82_N0.5420.92
120_L174_R0.5400.91
137_K140_T0.5380.91
40_N78_M0.5340.91
106_T129_T0.5310.91
208_A211_G0.5310.91
80_G195_W0.5300.91
207_L211_G0.5290.91
89_T219_L0.5280.91
67_P158_D0.5270.90
110_Y174_R0.5260.90
46_L61_T0.5190.90
108_A197_S0.5180.90
132_F151_V0.5180.90
27_R30_S0.5130.89
25_R86_R0.5110.89
43_R193_K0.5090.89
95_P172_S0.5080.89
20_V61_T0.5080.89
24_G220_E0.5070.89
132_F138_L0.5050.89
165_E170_K0.5040.88
26_A46_L0.5040.88
147_L225_D0.5030.88
155_F170_K0.5020.88
155_F179_V0.5020.88
143_S146_N0.5010.88
126_V188_T0.5000.88
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3phcA 5 0.9873 100 0.208 Contact Map
3bjeA 2 0.9958 100 0.209 Contact Map
1je0A 3 0.9619 100 0.21 Contact Map
1odkA 3 0.9831 100 0.218 Contact Map
4tymA 5 0.9788 100 0.225 Contact Map
2b94A 4 0.9449 100 0.228 Contact Map
4mchA 3 0.9703 100 0.229 Contact Map
3u40A 4 0.9788 100 0.231 Contact Map
4m3nA 3 0.9703 100 0.232 Contact Map
3mb8A 4 0.9831 100 0.234 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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