TRPB1 - SULSO - GREMLIN Contacts

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TRPB1 - Tryptophan synthase beta chain 1

UniProt:	P50383	Sequences: MVKEDEILPKYWYNIIPDLPKPLPPPRDPQGAYFSRIDLLRSILPKEVLRQQFTIERYIKIPEEVRDRYLSIGRPTPLFRAKRLEEYLKTPARIYFKYEGATPTGSHKINTAIPQAYFAKEEGIEHVVTETGAGQWGTAVALAASMYNMKSTIFMVKVSYEQKPMRRSIMQLYGANVYASPTNLTEYGRKILETNPQHPGSLGIAMSEAIEYALKNEFRYLVGSVLDVVLLHQSVIGQETITQLDLLGEDADILIGCVGGGSNFGGFTYPFIGNKKGKRYIAVSSAEIPKFSKGEYKYDFPDSAGLLPLVKMITLGKDYVPPPIYAGGLRYHGVAPTLSLLTKEGIVEWREYNEREIFEAAKIFIENQGIVPAPESAHAIRAVVDEAIEARKNNERKVIVFNLSGHGLLDLSNYESMMKRLNGNG	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	425 (358)
Sequences:	5721 (2930)
Seq/√Len:	154.9

ECOLI - ILVA SDHD TRPB DPAL TDCB CYSM CYSK DCYD
BACSU - ILVA SDHD TRPB THRC CYSM CYSK MCCA
HALSA - Q9HNH6 TRPB Q9HMQ7 Q9HRU4 Q9HNH0 Q9HQ70
SULSO - TRPB1 TRPB2 Q980P1 Q97ZL6 Q97ZT5 Q980F2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
240_T	400_V	4.922	1.00
144_A	151_S	4.651	1.00
279_R	348_E	4.556	1.00
356_E	385_D	3.710	1.00
80_R	93_R	3.699	1.00
253_I	281_I	3.380	1.00
52_Q	231_L	3.265	1.00
254_L	267_F	3.226	1.00
234_S	238_Q	3.054	1.00
157_K	186_E	3.034	1.00
154_F	202_L	3.026	1.00
78_L	400_V	2.878	1.00
152_T	176_N	2.813	1.00
217_E	265_G	2.769	1.00
93_R	398_V	2.686	1.00
152_T	178_Y	2.638	1.00
249_E	398_V	2.599	1.00
87_Y	363_I	2.548	1.00
150_K	176_N	2.541	1.00
267_F	402_N	2.505	1.00
300_F	342_T	2.448	1.00
157_K	190_K	2.435	1.00
283_V	382_A	2.415	1.00
80_R	85_E	2.343	1.00
241_I	274_N	2.337	1.00
202_L	206_M	2.304	1.00
240_T	267_F	2.254	1.00
253_I	279_R	2.236	1.00
198_H	201_S	2.200	1.00
80_R	95_Y	2.163	1.00
211_E	225_V	2.160	1.00
82_K	86_E	2.138	1.00
76_T	102_T	2.106	1.00
161_E	168_S	2.092	1.00
126_H	202_L	2.061	1.00
104_T	142_L	2.060	1.00
376_S	404_S	1.958	1.00
252_D	397_K	1.907	1.00
254_L	280_Y	1.899	1.00
390_A	397_K	1.890	1.00
237_G	270_P	1.889	1.00
186_E	190_K	1.888	1.00
83_R	86_E	1.865	1.00
286_A	353_N	1.861	1.00
75_P	242_T	1.843	1.00
96_F	364_F	1.837	1.00
237_G	267_F	1.828	1.00
201_S	205_A	1.824	1.00
313_I	343_K	1.821	1.00
244_L	400_V	1.777	1.00
281_I	348_E	1.772	1.00
363_I	384_V	1.758	1.00
55_I	121_E	1.743	1.00
158_V	161_E	1.738	1.00
362_K	418_M	1.727	1.00
242_T	245_D	1.720	1.00
353_N	356_E	1.702	1.00
338_L	349_W	1.687	1.00
88_L	384_V	1.684	1.00
350_R	382_A	1.681	1.00
244_L	398_V	1.656	1.00
93_R	247_L	1.656	1.00
361_A	377_A	1.652	1.00
376_S	403_L	1.630	1.00
119_A	123_G	1.611	1.00
51_Q	54_T	1.603	1.00
171_Q	176_N	1.600	1.00
72_I	102_T	1.582	1.00
116_A	213_A	1.573	1.00
355_R	359_E	1.560	1.00
360_A	381_R	1.544	1.00
304_A	311_K	1.542	1.00
87_Y	367_N	1.532	1.00
340_L	344_E	1.523	1.00
391_R	397_K	1.517	1.00
142_L	173_Y	1.493	1.00
352_Y	381_R	1.452	1.00
383_V	401_F	1.444	1.00
255_I	383_V	1.439	1.00
68_R	113_I	1.436	1.00
95_Y	398_V	1.431	1.00
358_F	374_P	1.429	1.00
313_I	342_T	1.427	1.00
272_I	347_V	1.417	1.00
267_F	280_Y	1.410	1.00
283_V	350_R	1.402	1.00
299_D	315_L	1.388	1.00
120_K	123_G	1.382	1.00
69_Y	72_I	1.380	1.00
223_G	227_D	1.356	1.00
282_A	349_W	1.351	1.00
206_M	209_A	1.351	1.00
126_H	150_K	1.344	1.00
244_L	247_L	1.339	1.00
79_F	98_Y	1.332	1.00
189_R	321_P	1.330	1.00
380_I	401_F	1.320	1.00
84_L	380_I	1.309	1.00
253_I	390_A	1.303	1.00
253_I	399_I	1.302	1.00
140_V	153_I	1.277	1.00
386_E	389_E	1.271	1.00
255_I	386_E	1.258	1.00
132_G	137_G	1.257	1.00
196_P	218_F	1.255	1.00
49_L	53_F	1.243	1.00
120_K	149_M	1.232	1.00
72_I	76_T	1.227	1.00
362_K	366_E	1.225	1.00
95_Y	247_L	1.221	1.00
44_L	207_S	1.216	1.00
254_L	271_F	1.215	1.00
312_M	339_S	1.212	1.00
95_Y	244_L	1.209	1.00
307_L	312_M	1.202	1.00
84_L	363_I	1.189	1.00
197_Q	201_S	1.187	1.00
124_I	219_R	1.186	1.00
412_S	416_S	1.181	1.00
130_E	153_I	1.177	1.00
382_A	386_E	1.177	1.00
286_A	351_E	1.175	1.00
283_V	352_Y	1.171	1.00
202_L	205_A	1.168	1.00
381_R	385_D	1.168	1.00
268_T	280_Y	1.167	1.00
253_I	386_E	1.166	1.00
391_R	395_E	1.159	1.00
160_Y	179_A	1.157	1.00
281_I	382_A	1.156	1.00
168_S	171_Q	1.151	1.00
196_P	200_G	1.144	1.00
359_E	363_I	1.143	1.00
62_P	65_V	1.143	1.00
255_I	283_V	1.135	1.00
205_A	209_A	1.131	1.00
50_R	54_T	1.130	1.00
118_F	228_V	1.127	1.00
116_A	127_V	1.123	1.00
358_F	417_M	1.122	1.00
46_K	50_R	1.118	1.00
88_L	363_I	1.112	1.00
253_I	397_K	1.109	0.99
116_A	147_Y	1.107	0.99
65_V	69_Y	1.106	0.99
379_A	401_F	1.103	0.99
360_A	380_I	1.101	0.99
104_T	109_I	1.093	0.99
93_R	396_R	1.089	0.99
127_V	144_A	1.077	0.99
196_P	214_L	1.076	0.99
60_K	63_E	1.072	0.99
88_L	388_I	1.072	0.99
84_L	94_I	1.066	0.99
66_R	238_Q	1.053	0.99
287_E	354_E	1.052	0.99
255_I	281_I	1.051	0.99
157_K	161_E	1.049	0.99
53_F	227_D	1.046	0.99
207_S	210_I	1.044	0.99
244_L	251_A	1.044	0.99
76_T	106_S	1.043	0.99
247_L	398_V	1.042	0.99
62_P	66_R	1.042	0.99
413_N	417_M	1.042	0.99
59_I	63_E	1.041	0.99
157_K	160_Y	1.041	0.99
168_S	415_E	1.036	0.99
127_V	152_T	1.034	0.99
363_I	367_N	1.032	0.99
112_A	143_A	1.032	0.99
388_I	391_R	1.031	0.99
240_T	402_N	1.025	0.99
238_Q	270_P	1.024	0.99
88_L	383_V	1.023	0.99
258_V	282_A	1.023	0.99
361_A	414_Y	1.021	0.99
83_R	367_N	1.019	0.99
53_F	57_R	1.013	0.99
250_D	278_K	1.012	0.99
312_M	333_G	1.007	0.99
214_L	217_E	1.006	0.99
325_Y	358_F	0.997	0.99
372_P	377_A	0.989	0.99
85_E	91_P	0.983	0.99
141_A	175_A	0.982	0.99
46_K	49_L	0.981	0.99
413_N	416_S	0.977	0.99
251_A	400_V	0.976	0.99
356_E	381_R	0.970	0.99
383_V	399_I	0.968	0.99
386_E	390_A	0.966	0.99
283_V	351_E	0.961	0.99
365_I	371_V	0.959	0.99
189_R	194_T	0.955	0.98
240_T	271_F	0.954	0.98
79_F	100_G	0.950	0.98
255_I	382_A	0.949	0.98
265_G	338_L	0.946	0.98
259_G	328_G	0.942	0.98
120_K	147_Y	0.941	0.98
125_E	150_K	0.940	0.98
169_I	371_V	0.933	0.98
290_K	293_K	0.932	0.98
281_I	386_E	0.929	0.98
82_K	146_M	0.920	0.98
157_K	179_A	0.920	0.98
160_Y	171_Q	0.917	0.98
372_P	376_S	0.917	0.98
268_T	345_G	0.915	0.98
252_D	398_V	0.915	0.98
249_E	252_D	0.914	0.98
197_Q	200_G	0.912	0.98
99_E	404_S	0.911	0.98
329_L	378_H	0.906	0.98
234_S	270_P	0.901	0.98
254_L	270_P	0.897	0.98
196_P	216_N	0.896	0.98
138_T	169_I	0.896	0.98
143_A	213_A	0.895	0.98
236_I	402_N	0.894	0.98
412_S	415_E	0.892	0.98
205_A	208_E	0.885	0.98
387_A	390_A	0.884	0.97
310_V	314_T	0.881	0.97
237_G	271_F	0.880	0.97
45_P	49_L	0.879	0.97
187_Y	194_T	0.875	0.97
68_R	146_M	0.875	0.97
168_S	172_L	0.872	0.97
56_E	231_L	0.869	0.97
218_F	310_V	0.868	0.97
230_L	337_T	0.867	0.97
98_Y	101_A	0.866	0.97
352_Y	382_A	0.865	0.97
167_R	177_V	0.863	0.97
47_E	50_R	0.861	0.97
200_G	203_G	0.861	0.97
61_I	65_V	0.860	0.97
364_F	380_I	0.853	0.97
75_P	243_Q	0.853	0.97
236_I	263_N	0.852	0.97
304_A	313_I	0.852	0.97
240_T	254_L	0.851	0.97
372_P	403_L	0.851	0.97
91_P	390_A	0.850	0.97
167_R	171_Q	0.849	0.97
51_Q	55_I	0.849	0.97
78_L	244_L	0.846	0.97
380_I	403_L	0.844	0.97
161_E	416_S	0.841	0.97
382_A	385_D	0.837	0.97
52_Q	232_H	0.836	0.96
64_E	120_K	0.835	0.96
245_D	248_G	0.834	0.96
275_K	278_K	0.830	0.96
140_V	144_A	0.817	0.96
227_D	231_L	0.817	0.96
119_A	127_V	0.816	0.96
172_L	362_K	0.813	0.96
281_I	350_R	0.812	0.96
44_L	47_E	0.810	0.96
194_T	198_H	0.810	0.96
62_P	69_Y	0.808	0.96
352_Y	385_D	0.807	0.96
53_F	56_E	0.806	0.96
411_L	415_E	0.805	0.96
55_I	58_Y	0.804	0.96
242_T	246_L	0.798	0.95
250_D	274_N	0.797	0.95
248_G	252_D	0.794	0.95
383_V	387_A	0.794	0.95
201_S	204_I	0.791	0.95
350_R	385_D	0.790	0.95
72_I	113_I	0.787	0.95
81_A	84_L	0.786	0.95
231_L	234_S	0.785	0.95
168_S	411_L	0.782	0.95
347_V	350_R	0.782	0.95
311_K	314_T	0.778	0.95
170_M	175_A	0.776	0.95
109_I	142_L	0.775	0.95
314_T	319_Y	0.773	0.95
52_Q	56_E	0.770	0.94
77_P	101_A	0.769	0.94
43_I	46_K	0.768	0.94
126_H	152_T	0.768	0.94
244_L	249_E	0.768	0.94
196_P	202_L	0.763	0.94
137_G	170_M	0.762	0.94
191_I	194_T	0.760	0.94
188_G	193_E	0.760	0.94
67_D	70_L	0.758	0.94
66_R	70_L	0.756	0.94
365_I	372_P	0.748	0.94
168_S	418_M	0.746	0.93
279_R	397_K	0.744	0.93
364_F	403_L	0.743	0.93
85_E	94_I	0.742	0.93
272_I	341_L	0.741	0.93
127_V	149_M	0.739	0.93
127_V	150_K	0.739	0.93
385_D	388_I	0.739	0.93
241_I	271_F	0.734	0.93
158_V	190_K	0.734	0.93
188_G	194_T	0.733	0.93
118_F	122_E	0.731	0.93
82_K	85_E	0.729	0.93
91_P	395_E	0.725	0.92
359_E	384_V	0.725	0.92
270_P	273_G	0.723	0.92
238_Q	242_T	0.721	0.92
370_I	403_L	0.721	0.92
155_M	170_M	0.718	0.92
85_E	92_A	0.718	0.92
163_K	327_G	0.717	0.92
376_S	379_A	0.713	0.92
116_A	143_A	0.710	0.92
290_K	334_V	0.710	0.92
196_P	201_S	0.705	0.91
298_Y	301_P	0.705	0.91
254_L	400_V	0.702	0.91
94_I	383_V	0.700	0.91
253_I	348_E	0.694	0.91
63_E	67_D	0.694	0.91
398_V	402_N	0.693	0.91
286_A	293_K	0.691	0.91
294_G	299_D	0.689	0.90
274_N	278_K	0.685	0.90
199_P	203_G	0.684	0.90
116_A	140_V	0.683	0.90
152_T	202_L	0.682	0.90
267_F	270_P	0.682	0.90
92_A	399_I	0.681	0.90
151_S	175_A	0.679	0.90
67_D	82_K	0.678	0.90
241_I	245_D	0.678	0.90
187_Y	190_K	0.675	0.90
107_H	263_N	0.675	0.90
116_A	149_M	0.675	0.90
377_A	380_I	0.671	0.89
109_I	146_M	0.668	0.89
167_R	179_A	0.667	0.89
390_A	399_I	0.664	0.89
120_K	125_E	0.664	0.89
258_V	334_V	0.664	0.89
92_A	390_A	0.663	0.89
250_D	271_F	0.663	0.89
169_I	411_L	0.662	0.89
132_G	373_A	0.661	0.89
288_I	293_K	0.661	0.89
91_P	391_R	0.661	0.89
55_I	60_K	0.659	0.88
84_L	87_Y	0.657	0.88
110_N	235_V	0.656	0.88
214_L	218_F	0.655	0.88
352_Y	357_I	0.654	0.88
169_I	173_Y	0.652	0.88
196_P	322_P	0.652	0.88
141_A	153_I	0.652	0.88
94_I	387_A	0.651	0.88
158_V	321_P	0.649	0.88
194_T	197_Q	0.648	0.88
187_Y	198_H	0.645	0.87
195_N	198_H	0.644	0.87
168_S	412_S	0.644	0.87
68_R	147_Y	0.643	0.87
356_E	359_E	0.641	0.87
129_T	203_G	0.640	0.87
375_E	378_H	0.640	0.87
142_L	146_M	0.639	0.87
200_G	204_I	0.635	0.87
229_V	233_Q	0.635	0.87
189_R	197_Q	0.635	0.87
364_F	372_P	0.633	0.86
372_P	380_I	0.632	0.86
188_G	191_I	0.631	0.86
246_L	249_E	0.631	0.86
197_Q	205_A	0.631	0.86
92_A	396_R	0.631	0.86
301_P	351_E	0.629	0.86
54_T	59_I	0.629	0.86
190_K	194_T	0.627	0.86
46_K	53_F	0.627	0.86
269_Y	337_T	0.626	0.86
50_R	53_F	0.626	0.86
282_A	338_L	0.625	0.86
306_L	333_G	0.623	0.85
228_V	232_H	0.621	0.85
353_N	385_D	0.621	0.85
58_Y	121_E	0.621	0.85
285_S	329_L	0.619	0.85
241_I	270_P	0.619	0.85
154_F	198_H	0.617	0.85
96_F	403_L	0.617	0.85
258_V	404_S	0.616	0.85
138_T	170_M	0.615	0.85
280_Y	346_I	0.615	0.85
364_F	370_I	0.615	0.85
265_G	337_T	0.615	0.85
157_K	189_R	0.614	0.85
265_G	269_Y	0.614	0.85
91_P	399_I	0.611	0.84
308_P	332_H	0.611	0.84
195_N	199_P	0.610	0.84
407_G	410_D	0.609	0.84
45_P	48_V	0.608	0.84
241_I	250_D	0.608	0.84
204_I	207_S	0.608	0.84
179_A	186_E	0.606	0.84
81_A	96_F	0.605	0.84
303_S	316_G	0.604	0.84
256_G	263_N	0.603	0.84
315_L	320_V	0.602	0.84
187_Y	201_S	0.602	0.84
360_A	384_V	0.601	0.83
278_K	346_I	0.601	0.83
144_A	213_A	0.598	0.83
306_L	311_K	0.598	0.83
231_L	269_Y	0.597	0.83
288_I	322_P	0.596	0.83
100_G	404_S	0.595	0.83
200_G	207_S	0.594	0.83
354_E	357_I	0.593	0.83
350_R	389_E	0.593	0.83
342_T	349_W	0.592	0.82
88_L	367_N	0.591	0.82
234_S	269_Y	0.591	0.82
198_H	202_L	0.588	0.82
163_K	373_A	0.588	0.82
294_G	318_D	0.588	0.82
92_A	383_V	0.588	0.82
102_T	109_I	0.585	0.82
238_Q	273_G	0.583	0.82
271_F	278_K	0.583	0.82
192_L	224_S	0.582	0.81
66_R	242_T	0.582	0.81
112_A	139_A	0.581	0.81
307_L	319_Y	0.581	0.81
92_A	397_K	0.578	0.81
414_Y	418_M	0.576	0.81
216_N	310_V	0.576	0.81
92_A	387_A	0.576	0.81
380_I	383_V	0.572	0.80
281_I	347_V	0.571	0.80
326_A	357_I	0.570	0.80
64_E	121_E	0.570	0.80
121_E	124_I	0.568	0.80
74_R	110_N	0.568	0.80
44_L	48_V	0.567	0.80
339_S	342_T	0.566	0.80
55_I	62_P	0.564	0.79
126_H	210_I	0.563	0.79
79_F	82_K	0.562	0.79
299_D	333_G	0.562	0.79
238_Q	274_N	0.560	0.79
56_E	62_P	0.558	0.79
66_R	235_V	0.557	0.79
163_K	374_P	0.556	0.78
203_G	209_A	0.555	0.78
133_A	163_K	0.554	0.78
77_P	312_M	0.554	0.78
199_P	206_M	0.552	0.78
48_V	273_G	0.552	0.78
169_I	172_L	0.552	0.78
133_A	373_A	0.551	0.78
84_L	364_F	0.550	0.78
230_L	269_Y	0.548	0.77
267_F	400_V	0.548	0.77
366_E	418_M	0.547	0.77
240_T	244_L	0.547	0.77
206_M	210_I	0.545	0.77
160_Y	180_S	0.545	0.77
91_P	388_I	0.545	0.77
258_V	376_S	0.545	0.77
312_M	319_Y	0.545	0.77
224_S	233_Q	0.544	0.77
342_T	346_I	0.544	0.77
133_A	331_Y	0.543	0.77
361_A	374_P	0.543	0.77
120_K	124_I	0.541	0.77
329_L	375_E	0.541	0.77
83_R	368_Q	0.540	0.76
110_N	233_Q	0.539	0.76
302_D	309_L	0.537	0.76
287_E	321_P	0.537	0.76
240_T	270_P	0.536	0.76
140_V	151_S	0.536	0.76
288_I	323_P	0.535	0.76
233_Q	376_S	0.533	0.75
99_E	376_S	0.532	0.75
356_E	360_A	0.532	0.75
390_A	396_R	0.529	0.75
96_F	380_I	0.529	0.75
314_T	320_V	0.528	0.75
66_R	69_Y	0.528	0.75
371_V	414_Y	0.527	0.75
136_W	233_Q	0.526	0.75
63_E	70_L	0.526	0.75
84_L	384_V	0.526	0.75
241_I	275_K	0.526	0.75
358_F	414_Y	0.525	0.74
63_E	242_T	0.521	0.74
89_K	395_E	0.521	0.74
119_A	225_V	0.521	0.74
127_V	213_A	0.521	0.74
211_E	316_G	0.520	0.74
300_F	349_W	0.519	0.74
163_K	405_G	0.518	0.73
207_S	211_E	0.518	0.73
272_I	280_Y	0.518	0.73
218_F	312_M	0.517	0.73
95_Y	249_E	0.516	0.73
340_L	343_K	0.516	0.73
237_G	274_N	0.515	0.73
119_A	149_M	0.514	0.73
78_L	81_A	0.513	0.73
190_K	193_E	0.513	0.73
376_S	380_I	0.512	0.73
89_K	396_R	0.512	0.73
226_L	336_P	0.511	0.72
244_L	250_D	0.511	0.72
57_R	62_P	0.509	0.72
333_G	336_P	0.509	0.72
98_Y	403_L	0.508	0.72
214_L	312_M	0.508	0.72
342_T	347_V	0.507	0.72
88_L	387_A	0.505	0.72
200_G	205_A	0.504	0.71
334_V	342_T	0.504	0.71
291_F	294_G	0.504	0.71
194_T	201_S	0.503	0.71
304_A	339_S	0.503	0.71
307_L	310_V	0.503	0.71
131_T	375_E	0.502	0.71
85_E	93_R	0.501	0.71
196_P	199_P	0.500	0.71

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4qysB	2	0.9576	100	0.296	Contact Map
3ss7X	1	0.9059	100	0.341	Contact Map
1tdjA	2	0.7741	100	0.351	Contact Map
2gn0A	2	0.7694	100	0.371	Contact Map
1v71A	2	0.7435	100	0.378	Contact Map
2d1fA	2	0.7694	100	0.382	Contact Map
2zsjA	2	0.7647	100	0.383	Contact Map
3aeyA	2	0.7624	100	0.383	Contact Map
4negA	2	0.76	100	0.384	Contact Map
1x1qA	2	0.9035	100	0.385	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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