GREMLIN Database
FUMC - Fumarate hydratase class II
UniProt: P39461 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 438 (395)
Sequences: 11011 (5907)
Seq/√Len: 297.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
38_A48_A3.8231.00
31_K56_D3.4561.00
47_I105_H3.3451.00
39_D221_E3.2681.00
370_K373_R3.2361.00
35_K52_I3.2151.00
35_K225_I2.9101.00
137_Q184_H2.8371.00
25_S126_A2.8091.00
281_D365_V2.7671.00
417_S420_E2.7391.00
256_A313_V2.7321.00
140_I181_Q2.7111.00
133_L188_M2.6611.00
372_K376_E2.6501.00
128_V248_L2.6091.00
142_S362_D2.5901.00
130_N191_L2.5451.00
21_R347_N2.4971.00
193_Y247_L2.4901.00
169_L366_A2.4561.00
316_L345_Q2.4541.00
221_E224_R2.4361.00
133_L184_H2.3821.00
281_D367_N2.3721.00
146_K149_E2.3011.00
309_I352_A2.2431.00
125_V195_K2.2381.00
38_A45_K2.2311.00
44_D47_I2.2011.00
253_R317_D2.1981.00
195_K229_G2.1101.00
283_P363_G2.0961.00
152_D368_V2.0911.00
373_R377_S2.0551.00
135_A354_E2.0501.00
137_Q188_M2.0421.00
312_Q341_N2.0131.00
140_I184_H2.0051.00
45_K49_D1.9801.00
138_Q358_R1.9681.00
145_K149_E1.9531.00
142_S357_S1.9281.00
189_N193_Y1.9271.00
145_K362_D1.9251.00
370_K374_Y1.9191.00
263_G306_T1.8971.00
221_E225_I1.8941.00
57_D91_V1.8831.00
257_V314_V1.8541.00
184_H188_M1.8501.00
136_L255_I1.8461.00
418_D422_N1.8421.00
49_D53_K1.8391.00
28_V126_A1.8361.00
405_M413_E1.8341.00
186_Q247_L1.8311.00
142_S361_I1.8281.00
91_V95_Y1.7901.00
197_L223_N1.7641.00
372_K428_S1.7641.00
189_N192_E1.7611.00
408_R422_N1.7591.00
144_N181_Q1.7531.00
31_K52_I1.7321.00
420_E423_K1.7271.00
150_Y153_V1.7241.00
393_K396_E1.7211.00
426_D429_K1.7001.00
400_K414_L1.6781.00
259_L306_T1.6541.00
183_E258_D1.6531.00
140_I177_A1.6511.00
357_S362_D1.6511.00
368_V372_K1.6321.00
48_A52_I1.6211.00
35_K221_E1.6141.00
182_H237_R1.6081.00
412_R418_D1.5351.00
138_Q357_S1.5211.00
220_N224_R1.5191.00
199_I218_V1.5181.00
405_M410_A1.5051.00
36_V221_E1.4971.00
147_A177_A1.4881.00
135_A350_S1.4781.00
319_A337_L1.4691.00
201_G238_A1.4631.00
146_K150_Y1.4561.00
246_L324_S1.4521.00
57_D98_L1.4511.00
182_H243_T1.4441.00
403_K410_A1.4401.00
389_I397_I1.4241.00
400_K403_K1.4211.00
190_I251_A1.4201.00
192_E229_G1.4191.00
84_V88_I1.3731.00
303_V360_V1.3681.00
96_S99_K1.3661.00
358_R362_D1.3621.00
369_E373_R1.3471.00
127_E350_S1.3411.00
24_W28_V1.3401.00
242_L335_I1.3391.00
144_N148_E1.3381.00
185_E193_Y1.3201.00
367_N370_K1.3191.00
31_K35_K1.3111.00
138_Q362_D1.3041.00
253_R321_Q1.3001.00
268_L328_E1.2991.00
263_G356_M1.2951.00
82_M85_N1.2941.00
250_G254_N1.2911.00
410_A414_L1.2901.00
133_L191_L1.2891.00
246_L254_N1.2791.00
124_A248_L1.2781.00
318_H322_F1.2711.00
21_R127_E1.2701.00
140_I180_F1.2681.00
406_S409_E1.2661.00
169_L173_L1.2601.00
173_L176_Y1.2551.00
176_Y330_E1.2421.00
27_G58_L1.2361.00
34_A43_L1.2361.00
142_S145_K1.2341.00
136_L180_F1.2131.00
26_M115_T1.2111.00
219_I223_N1.2051.00
153_V368_V1.1911.00
42_L108_F1.1831.00
50_S53_K1.1821.00
144_N177_A1.1711.00
148_E151_K1.1631.00
312_Q344_T1.1631.00
187_V255_I1.1601.00
109_G210_S1.1591.00
387_P420_E1.1521.00
405_M414_L1.1521.00
16_T344_T1.1511.00
163_D276_G1.1441.00
278_N370_K1.1411.00
27_G84_V1.1401.00
223_N229_G1.1331.00
93_S97_N1.1301.00
169_L371_M1.1301.00
124_A342_I1.1281.00
150_Y368_V1.1271.00
135_A138_Q1.1261.00
74_T85_N1.1241.00
109_G211_H1.1241.00
268_L277_L1.1231.00
151_K170_G1.1211.00
139_I180_F1.1181.00
135_A357_S1.1151.00
45_K48_A1.1141.00
405_M409_E1.1041.00
29_L115_T1.1041.00
389_I394_A1.1031.00
320_N342_I1.1031.00
306_T352_A1.0981.00
143_L262_L1.0971.00
155_K371_M1.0941.00
32_S225_I1.0861.00
266_I303_V1.0831.00
133_L137_Q1.0821.00
138_Q354_E1.0821.00
125_V228_L1.0811.00
176_Y262_L1.0801.00
128_V191_L1.0741.00
245_L342_I1.0731.00
315_G319_A1.0701.00
83_N86_E1.0681.00
52_I56_D1.0681.00
137_Q141_S1.0661.00
280_I364_M1.0561.00
49_D52_I1.0541.00
185_E192_E1.0481.00
94_S97_N1.0481.00
393_K416_Y1.0461.00
118_T197_L1.0441.00
408_R412_R1.0421.00
39_D225_I1.0421.00
153_V371_M1.0421.00
139_I361_I1.0401.00
50_S54_A1.0361.00
69_L72_F1.0351.00
166_P375_A1.0341.00
53_K95_Y1.0311.00
152_D170_G1.0261.00
310_S314_V1.0251.00
157_G167_V1.0251.00
411_L414_L1.0231.00
25_S122_I1.0211.00
120_I339_G1.0201.00
131_R350_S1.0171.00
24_W126_A1.0161.00
174_S234_N1.0161.00
127_E131_R1.0161.00
251_A255_I1.0151.00
409_E413_E1.0141.00
195_K228_L1.0111.00
196_E230_F1.0101.00
28_V225_I1.0091.00
167_V204_T1.0091.00
20_R24_W1.0071.00
120_I245_L1.0061.00
146_K361_I1.0051.00
314_V322_F1.0041.00
92_A100_V1.0031.00
260_Y307_L0.9971.00
249_S320_N0.9921.00
146_K173_L0.9901.00
191_L195_K0.9891.00
388_V424_I0.9881.00
125_V191_L0.9861.00
160_H304_E0.9811.00
268_L329_F0.9791.00
151_K168_T0.9771.00
259_L353_L0.9671.00
410_A413_E0.9661.00
54_A57_D0.9641.00
136_L184_H0.9591.00
178_D182_H0.9571.00
248_L342_I0.9561.00
419_N423_K0.9561.00
172_E330_E0.9541.00
196_E231_K0.9541.00
278_N367_N0.9531.00
266_I364_M0.9511.00
388_V420_E0.9511.00
44_D105_H0.9471.00
13_N305_A0.9461.00
38_A49_D0.9391.00
168_T431_V0.9391.00
50_S102_P0.9361.00
128_V187_V0.9321.00
26_M29_L0.9251.00
189_N247_L0.9251.00
40_L211_H0.9231.00
185_E189_N0.9221.00
96_S103_N0.9221.00
70_D74_T0.9171.00
403_K414_L0.9171.00
29_L118_T0.9151.00
67_I73_Q0.9101.00
143_L173_L0.9001.00
158_R276_G0.9001.00
375_A427_L0.9001.00
21_R126_A0.8971.00
197_L222_I0.8961.00
71_V93_S0.8961.00
140_I144_N0.8861.00
36_V40_L0.8841.00
312_Q340_Y0.8811.00
153_V366_A0.8801.00
25_S123_A0.8801.00
55_S58_L0.8781.00
158_R163_D0.8771.00
385_V388_V0.8771.00
149_E365_V0.8741.00
46_K49_D0.8721.00
47_I51_I0.8701.00
147_A174_S0.8591.00
400_K410_A0.8581.00
317_D321_Q0.8561.00
47_I100_V0.8511.00
163_D275_T0.8471.00
249_S317_D0.8471.00
67_I72_F0.8451.00
250_G317_D0.8451.00
218_V223_N0.8441.00
101_H104_D0.8431.00
408_R418_D0.8391.00
161_L268_L0.8381.00
249_S342_I0.8371.00
68_V71_V0.8351.00
28_V222_I0.8321.00
26_M84_V0.8311.00
167_V236_F0.8311.00
143_L361_I0.8301.00
154_I375_A0.8281.00
82_M86_E0.8261.00
406_S410_A0.8241.00
154_I431_V0.8241.00
171_Q235_R0.8241.00
53_K57_D0.8231.00
255_I353_L0.8221.00
33_C110_Q0.8211.00
177_A181_Q0.8201.00
354_E358_R0.8191.00
36_V218_V0.8181.00
141_S145_K0.8171.00
126_A130_N0.8161.00
85_N88_I0.8141.00
316_L344_T0.8121.00
318_H321_Q0.8111.00
340_Y344_T0.8101.00
400_K413_E0.8071.00
257_V324_S0.8061.00
178_D233_A0.8031.00
306_T356_M0.8021.00
262_L356_M0.8021.00
39_D45_K0.8001.00
163_D378_S0.8001.00
312_Q316_L0.7991.00
142_S146_K0.7991.00
143_L177_A0.7981.00
120_I342_I0.7981.00
198_P238_A0.7981.00
34_A48_A0.7960.99
69_L73_Q0.7940.99
247_L250_G0.7940.99
196_E244_D0.7930.99
241_L258_D0.7910.99
182_H196_E0.7890.99
150_Y366_A0.7790.99
314_V318_H0.7780.99
91_V94_S0.7770.99
260_Y325_M0.7750.99
172_E269_M0.7750.99
47_I102_P0.7740.99
396_E400_K0.7730.99
240_R330_E0.7660.99
94_S98_L0.7620.99
156_A275_T0.7610.99
257_V321_Q0.7580.99
360_V364_M0.7580.99
202_T293_I0.7550.99
35_K39_D0.7540.99
311_A323_A0.7530.99
376_E408_R0.7530.99
38_A43_L0.7470.99
31_K225_I0.7470.99
51_I55_S0.7450.99
248_L347_N0.7440.99
155_K172_E0.7440.99
286_E363_G0.7430.99
223_N231_K0.7420.99
149_E368_V0.7410.99
192_E195_K0.7380.99
68_V72_F0.7370.99
152_D428_S0.7350.99
22_I116_V0.7340.99
125_V130_N0.7270.99
233_A238_A0.7240.99
65_D68_V0.7240.99
263_G303_V0.7230.99
323_A337_L0.7230.99
128_V190_I0.7220.99
136_L353_L0.7190.99
46_K50_S0.7180.99
31_K48_A0.7170.99
243_T258_D0.7160.99
180_F353_L0.7160.99
34_A51_I0.7150.99
143_L180_F0.7100.99
51_I102_P0.7100.99
353_L357_S0.7090.99
319_A341_N0.7070.99
421_I425_L0.7050.99
311_A319_A0.7040.99
236_F330_E0.7030.99
423_K429_K0.7030.99
314_V321_Q0.7030.99
32_S35_K0.7010.99
248_L252_L0.7000.99
153_V168_T0.7000.99
366_A371_M0.6980.99
179_A241_L0.6960.99
412_R422_N0.6950.99
89_A93_S0.6950.99
141_S144_N0.6950.99
389_I393_K0.6950.99
419_N422_N0.6930.99
269_M329_F0.6930.99
93_S96_S0.6880.99
402_N414_L0.6880.99
90_E93_S0.6870.99
180_F183_E0.6860.99
420_E424_I0.6860.99
179_A258_D0.6860.99
199_I222_I0.6850.98
369_E372_K0.6830.98
402_N410_A0.6830.98
237_R243_T0.6820.98
39_D211_H0.6820.98
173_L371_M0.6800.98
316_L346_V0.6800.98
312_Q348_F0.6760.98
260_Y326_L0.6740.98
175_A261_R0.6730.98
264_Q307_L0.6720.98
13_N17_K0.6700.98
428_S432_K0.6690.98
18_F340_Y0.6680.98
262_L361_I0.6660.98
131_R135_A0.6660.98
257_V325_M0.6640.98
147_A151_K0.6640.98
153_V372_K0.6630.98
185_E188_M0.6610.98
405_M411_L0.6600.98
145_K148_E0.6590.98
409_E418_D0.6580.98
43_L108_F0.6570.98
150_Y169_L0.6560.98
130_N228_L0.6560.98
132_L346_V0.6560.98
183_E254_N0.6550.98
125_V229_G0.6550.98
57_D95_Y0.6540.98
390_G393_K0.6510.98
260_Y322_F0.6500.98
38_A52_I0.6490.98
87_V90_E0.6480.98
34_A106_V0.6470.98
342_I346_V0.6460.98
155_K167_V0.6450.98
156_A375_A0.6450.98
195_K223_N0.6440.98
137_Q181_Q0.6440.98
139_I357_S0.6430.98
319_A323_A0.6420.98
42_L211_H0.6390.98
70_D73_Q0.6380.98
40_L221_E0.6380.98
132_L350_S0.6370.98
128_V133_L0.6370.98
71_V74_T0.6370.98
186_Q243_T0.6360.98
302_T356_M0.6340.98
17_K127_E0.6330.97
148_E152_D0.6310.97
138_Q142_S0.6300.97
27_G55_S0.6290.97
101_H392_D0.6290.97
127_E346_V0.6280.97
121_R244_D0.6280.97
404_G410_A0.6270.97
137_Q140_I0.6270.97
128_V194_V0.6250.97
396_E414_L0.6210.97
123_A346_V0.6210.97
86_E89_A0.6200.97
190_I248_L0.6190.97
246_L250_G0.6170.97
142_S358_R0.6160.97
259_L357_S0.6150.97
253_R257_V0.6140.97
134_P138_Q0.6130.97
255_I349_I0.6130.97
139_I353_L0.6130.97
132_L252_L0.6120.97
35_K48_A0.6110.97
397_I411_L0.6110.97
237_R258_D0.6090.97
245_L320_N0.6090.97
203_A293_I0.6080.97
102_P105_H0.6070.97
423_K426_D0.6070.97
42_L109_G0.6060.97
246_L320_N0.6040.97
48_A51_I0.6030.97
370_K377_S0.6030.97
306_T360_V0.6030.97
178_D234_N0.6020.97
190_I194_V0.6010.97
88_I92_A0.6000.97
47_I50_S0.5970.96
324_S335_I0.5960.96
376_E406_S0.5960.96
395_T399_K0.5940.96
286_E358_R0.5920.96
67_I90_E0.5920.96
43_L51_I0.5910.96
186_Q189_N0.5910.96
222_I225_I0.5900.96
180_F255_I0.5890.96
168_T432_K0.5880.96
375_A380_S0.5870.96
408_R414_L0.5860.96
374_Y377_S0.5840.96
183_E186_Q0.5820.96
105_H108_F0.5810.96
55_S88_I0.5810.96
248_L346_V0.5800.96
187_V251_A0.5800.96
29_L199_I0.5790.96
181_Q185_E0.5790.96
176_Y179_A0.5780.96
16_T340_Y0.5780.96
341_N344_T0.5760.96
307_L334_G0.5760.96
29_L119_A0.5750.96
368_V371_M0.5740.96
408_R411_L0.5730.96
250_G253_R0.5730.96
144_N147_A0.5730.96
252_L349_I0.5720.95
155_K269_M0.5720.95
195_K230_F0.5700.95
146_K364_M0.5690.95
85_N89_A0.5650.95
180_F259_L0.5630.95
220_N223_N0.5610.95
400_K405_M0.5610.95
28_V226_T0.5580.95
28_V32_S0.5570.95
132_L349_I0.5570.95
25_S28_V0.5570.95
379_P382_I0.5570.95
43_L47_I0.5540.95
124_A194_V0.5540.95
186_Q193_Y0.5540.95
58_L88_I0.5530.95
179_A240_R0.5520.95
197_L231_K0.5510.94
302_T360_V0.5490.94
277_L374_Y0.5490.94
417_S422_N0.5490.94
167_V171_Q0.5490.94
403_K413_E0.5490.94
192_E196_E0.5490.94
133_L251_A0.5490.94
218_V221_E0.5450.94
136_L187_V0.5450.94
253_R318_H0.5430.94
263_G307_L0.5420.94
309_I348_F0.5420.94
16_T21_R0.5410.94
172_E265_D0.5410.94
311_A322_F0.5410.94
403_K411_L0.5410.94
192_E231_K0.5400.94
264_Q326_L0.5400.94
392_D396_E0.5400.94
243_T246_L0.5390.94
163_D380_S0.5380.94
68_V73_Q0.5380.94
183_E251_A0.5370.94
51_I95_Y0.5370.94
190_I247_L0.5360.94
178_D181_Q0.5360.94
247_L254_N0.5320.93
67_I94_S0.5320.93
185_E196_E0.5320.93
429_K432_K0.5310.93
128_V195_K0.5310.93
416_Y420_E0.5290.93
154_I427_L0.5280.93
270_F280_I0.5260.93
252_L255_I0.5250.93
256_A349_I0.5250.93
186_Q254_N0.5240.93
121_R245_L0.5240.93
249_S345_Q0.5230.93
156_A374_Y0.5230.93
182_H247_L0.5220.93
160_H333_M0.5220.93
139_I358_R0.5200.93
339_G343_V0.5190.93
174_S177_A0.5190.93
404_G411_L0.5180.92
173_L364_M0.5180.92
223_N228_L0.5170.92
139_I142_S0.5170.92
412_R421_I0.5150.92
218_V222_I0.5150.92
428_S431_V0.5140.92
252_L313_V0.5140.92
385_V389_I0.5140.92
21_R131_R0.5130.92
48_A55_S0.5130.92
198_P239_M0.5110.92
38_A44_D0.5080.92
33_C222_I0.5080.92
23_I87_V0.5080.92
93_S99_K0.5070.92
133_L136_L0.5060.92
407_I414_L0.5060.92
161_L328_E0.5040.91
266_I361_I0.5030.91
164_A204_T0.5030.91
69_L87_V0.5020.91
110_Q115_T0.5020.91
188_M192_E0.5010.91
34_A52_I0.5000.91
43_L48_A0.5000.91
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1furA 4 0.9703 100 0.153 Contact Map
1vdkA 4 0.9795 100 0.157 Contact Map
1yfmA 4 0.9658 100 0.16 Contact Map
3gtdA 4 0.9726 100 0.161 Contact Map
4hgvA 4 0.984 100 0.162 Contact Map
4admA 4 0.9772 100 0.164 Contact Map
3e04A 4 0.984 100 0.167 Contact Map
1jswA 4 0.9658 100 0.167 Contact Map
3r6qA 4 0.9863 100 0.169 Contact Map
3ocfA 4 0.9498 100 0.171 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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