GREMLIN Database
HIS4 - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
UniProt: O33772 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 232 (225)
Sequences: 5794 (3075)
Seq/√Len: 205.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
88_A115_I3.3561.00
139_S143_E2.6201.00
113_Y158_L2.5381.00
19_I173_T2.5341.00
128_I152_I2.4361.00
156_N190_G2.4081.00
84_D87_K2.3861.00
126_V155_V2.3361.00
108_N114_N2.3161.00
135_R144_R2.3001.00
37_N71_I2.2981.00
154_K158_L2.2921.00
14_K28_V2.2551.00
89_K93_S2.1961.00
37_N41_N2.1691.00
107_T139_S2.1521.00
135_R147_E2.0901.00
38_K42_E2.0421.00
202_S205_T2.0291.00
174_V225_K1.9941.00
202_S206_F1.9921.00
40_Y73_F1.9661.00
227_R230_N1.9381.00
115_I124_V1.8821.00
62_E90_R1.8731.00
82_I91_L1.8631.00
88_A124_V1.8031.00
106_F138_I1.7901.00
147_E150_D1.7641.00
31_N34_E1.7291.00
156_N189_R1.7151.00
20_R173_T1.7111.00
89_K118_E1.7091.00
182_K186_K1.6811.00
85_V115_I1.6741.00
217_V221_F1.6501.00
179_Y206_F1.6071.00
201_D226_I1.5821.00
42_E229_T1.5791.00
31_N67_E1.5621.00
198_V207_L1.5581.00
47_I74_D1.5431.00
146_M154_K1.5391.00
153_K187_L1.5281.00
12_F53_D1.5211.00
61_N64_Y1.5051.00
127_S164_I1.4981.00
220_A226_I1.4941.00
217_V220_A1.4901.00
200_S203_D1.4881.00
101_F104_I1.4871.00
98_A125_I1.4851.00
63_M66_K1.4781.00
154_K157_E1.4511.00
110_N114_N1.4491.00
137_L144_R1.4471.00
185_A192_K1.4221.00
104_I111_L1.4211.00
69_C76_I1.4151.00
92_I123_R1.4141.00
85_V118_E1.4051.00
22_V25_T1.3921.00
206_F209_N1.3811.00
5_I46_R1.3681.00
67_E70_K1.3621.00
6_P44_Y1.3531.00
85_V114_N1.3261.00
130_Y165_L1.3211.00
203_D206_F1.3201.00
167_Y178_D1.3141.00
19_I25_T1.3081.00
46_R98_A1.2941.00
113_Y117_R1.2821.00
49_V68_I1.2801.00
186_K211_G1.2691.00
86_E89_K1.2671.00
88_A99_L1.2671.00
105_V126_V1.2661.00
204_I220_A1.2491.00
3_E46_R1.2341.00
226_I231_V1.2291.00
38_K41_N1.2221.00
65_I78_V1.2121.00
204_I217_V1.2111.00
42_E227_R1.2071.00
66_K70_K1.2001.00
152_I184_Y1.1891.00
165_L184_Y1.1881.00
182_K209_N1.1841.00
127_S166_T1.1841.00
66_K94_L1.1671.00
125_I164_I1.1581.00
138_I143_E1.1441.00
38_K224_N1.1391.00
106_F146_M1.1361.00
103_T107_T1.1361.00
164_I216_I1.1361.00
207_L215_I1.1311.00
149_I187_L1.1271.00
201_D231_V1.1221.00
132_D169_T1.1131.00
161_L191_V1.1031.00
11_S23_R1.0991.00
3_E45_S1.0991.00
183_D186_K1.0911.00
15_A35_L1.0811.00
163_I190_G1.0801.00
184_Y192_K1.0741.00
153_K157_E1.0691.00
177_I198_V1.0651.00
126_V160_L1.0631.00
46_R75_W1.0591.00
207_L212_F1.0581.00
181_V185_A1.0491.00
133_T137_L1.0471.00
107_T138_I1.0411.00
29_L35_L1.0311.00
179_Y182_K1.0301.00
33_I64_Y1.0281.00
78_V91_L1.0271.00
130_Y148_I1.0251.00
110_N113_Y1.0231.00
183_D187_L1.0161.00
107_T143_E1.0121.00
89_K119_I0.9981.00
205_T209_N0.9971.00
179_Y210_V0.9941.00
69_C96_V0.9941.00
156_N188_I0.9921.00
109_F154_K0.9911.00
111_L115_I0.9801.00
162_G191_V0.9781.00
201_D205_T0.9781.00
152_I188_I0.9691.00
148_I184_Y0.9661.00
125_I162_G0.9600.99
116_V160_L0.9590.99
78_V96_V0.9560.99
35_L38_K0.9550.99
33_I67_E0.9500.99
132_D180_S0.9460.99
148_I152_I0.9390.99
86_E90_R0.9360.99
46_R125_I0.9270.99
55_A103_T0.9220.99
185_A212_F0.9170.99
112_F126_V0.9090.99
59_G90_R0.9030.99
14_K30_G0.9030.99
69_C97_N0.9030.99
17_K219_M0.9020.99
4_I217_V0.8940.99
182_K210_V0.8920.99
40_Y72_G0.8870.99
77_Q100_I0.8820.99
37_N40_Y0.8810.99
149_I183_D0.8810.99
72_G75_W0.8760.99
201_D230_N0.8690.99
101_F115_I0.8670.99
39_L222_Y0.8640.99
150_D154_K0.8640.99
149_I153_K0.8630.99
92_I119_I0.8620.99
138_I145_S0.8590.99
90_R94_L0.8580.99
219_M223_L0.8580.99
65_I96_V0.8570.99
131_D169_T0.8550.99
185_A210_V0.8540.99
131_D137_L0.8540.99
63_M67_E0.8510.99
66_K90_R0.8460.99
50_V164_I0.8430.99
198_V215_I0.8360.99
59_G87_K0.8350.99
185_A213_D0.8250.98
199_S203_D0.8240.98
208_K211_G0.8180.98
45_S75_W0.8150.98
205_T208_K0.8150.98
112_F155_V0.8070.98
88_A119_I0.8060.98
113_Y154_K0.8020.98
106_F151_G0.8010.98
75_W97_N0.7880.98
11_S53_D0.7880.98
85_V119_I0.7880.98
200_S204_I0.7870.98
42_E228_G0.7870.98
162_G213_D0.7860.98
180_S183_D0.7850.98
200_S225_K0.7830.98
67_E71_I0.7800.98
54_A60_N0.7800.98
85_V89_K0.7770.98
19_I219_M0.7740.98
40_Y74_D0.7720.98
137_L168_V0.7710.98
87_K90_R0.7650.97
3_E214_Y0.7620.97
149_I152_I0.7620.97
11_S24_G0.7600.97
59_G83_R0.7590.97
133_T144_R0.7540.97
53_D57_G0.7530.97
33_I71_I0.7520.97
207_L210_V0.7500.97
191_V213_D0.7480.97
133_T183_D0.7430.97
150_D153_K0.7410.97
29_L223_L0.7390.97
170_N175_K0.7380.97
227_R231_V0.7360.97
122_N161_L0.7350.97
128_I151_G0.7230.96
62_E66_K0.7210.96
50_V166_T0.7180.96
98_A161_L0.7170.96
5_I193_E0.7150.96
153_K156_N0.7130.96
117_R157_E0.7120.96
156_N163_I0.7120.96
181_V192_K0.7120.96
108_N111_L0.7100.96
193_E214_Y0.7100.96
34_E38_K0.7020.96
5_I125_I0.6990.96
4_I215_I0.6970.96
136_V151_G0.6970.96
170_N173_T0.6960.96
133_T147_E0.6950.96
116_V120_G0.6850.95
69_C94_L0.6820.95
120_G162_G0.6800.95
204_I226_I0.6800.95
62_E91_L0.6760.95
23_R142_K0.6750.95
56_E60_N0.6730.95
204_I215_I0.6610.94
82_I88_A0.6590.94
30_G35_L0.6590.94
100_I164_I0.6580.94
126_V163_I0.6550.94
152_I163_I0.6550.94
97_N122_N0.6500.94
68_I76_I0.6460.93
88_A101_F0.6460.93
82_I101_F0.6450.93
44_Y228_G0.6440.93
164_I193_E0.6430.93
104_I108_N0.6430.93
114_N117_R0.6400.93
88_A123_R0.6400.93
64_Y68_I0.6390.93
204_I221_F0.6380.93
66_K93_S0.6380.93
221_F231_V0.6370.93
88_A92_I0.6350.93
5_I77_Q0.6310.93
184_Y188_I0.6270.92
185_A211_G0.6190.92
53_D61_N0.6180.92
36_A68_I0.6170.92
34_E37_N0.6150.92
60_N65_I0.6150.92
97_N123_R0.6130.92
181_V194_Y0.6130.92
218_G222_Y0.6130.92
181_V207_L0.6110.91
177_I199_S0.6110.91
90_R93_S0.6110.91
128_I136_V0.6100.91
59_G91_L0.6050.91
101_F112_F0.6040.91
10_I49_V0.5970.91
10_I68_I0.5960.90
138_I142_K0.5950.90
104_I115_I0.5940.90
17_K171_E0.5940.90
49_V76_I0.5930.90
44_Y231_V0.5920.90
132_D178_D0.5910.90
56_E59_G0.5890.90
153_K188_I0.5880.90
98_A162_G0.5860.90
198_V217_V0.5840.90
57_G61_N0.5830.89
120_G123_R0.5790.89
210_V213_D0.5750.89
146_M150_D0.5720.89
204_I231_V0.5680.88
86_E93_S0.5660.88
78_V82_I0.5650.88
199_S225_K0.5640.88
162_G193_E0.5630.88
10_I64_Y0.5600.88
54_A59_G0.5600.88
5_I98_A0.5540.87
35_L39_L0.5520.87
116_V126_V0.5520.87
83_R107_T0.5500.87
172_G197_G0.5440.86
177_I203_D0.5440.86
159_E190_G0.5430.86
38_K229_T0.5420.86
3_E213_D0.5400.86
20_R25_T0.5390.86
3_E122_N0.5320.85
166_T195_A0.5320.85
59_G62_E0.5310.85
18_R24_G0.5290.84
113_Y157_E0.5290.84
18_R141_W0.5270.84
16_V21_G0.5270.84
198_V226_I0.5270.84
72_G76_I0.5270.84
64_Y76_I0.5270.84
18_R102_S0.5260.84
177_I204_I0.5240.84
51_D57_G0.5230.84
172_G176_G0.5210.84
198_V204_I0.5160.83
25_T223_L0.5160.83
127_S193_E0.5160.83
20_R169_T0.5160.83
54_A58_V0.5160.83
181_V212_F0.5160.83
7_S166_T0.5120.83
50_V127_S0.5090.82
115_I119_I0.5090.82
105_V112_F0.5080.82
7_S216_I0.5070.82
36_A72_G0.5070.82
32_P64_Y0.5060.82
149_I188_I0.5030.81
176_G197_G0.5010.81
152_I187_L0.5000.81
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2agkA 1 0.8836 100 0.266 Contact Map
1jvnA 1 0.9569 100 0.302 Contact Map
4gj1A 1 0.931 100 0.448 Contact Map
1qo2A 1 0.9784 100 0.449 Contact Map
2y88A 1 0.9914 100 0.474 Contact Map
4x2rA 1 0.9698 100 0.491 Contact Map
4u28A 1 0.9655 100 0.497 Contact Map
1thfD 2 0.9914 100 0.512 Contact Map
4axkA 1 0.9784 100 0.517 Contact Map
4evzA 1 0.9914 100 0.522 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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