GREMLIN Database
AmpE - Regulatory signalling modulator protein AmpE
PFAM: PF17113 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 284 (281)
Sequences: 792 (575)
Seq/√Len: 34.3
META: 0.593

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
162_Y165_Y6.7321.00
159_A162_Y5.5261.00
139_A142_R5.0281.00
140_N143_R4.7331.00
159_A163_R4.1191.00
163_R166_A3.7971.00
162_Y166_A3.4551.00
159_A165_Y3.3821.00
155_G159_A3.1761.00
139_A143_R3.1321.00
156_P159_A2.8971.00
159_A166_A2.7311.00
160_V167_L2.6611.00
19_G23_Q2.6351.00
47_A50_P2.5771.00
18_L23_Q2.5581.00
138_W143_R2.5471.00
18_L22_W2.4201.00
138_W144_Y2.4191.00
160_V166_A2.3860.99
160_V163_R2.3660.99
17_T23_Q2.3280.99
140_N144_Y2.2500.99
132_V135_A2.1060.99
19_G22_W2.0670.98
78_L83_G2.0480.98
163_R167_L1.9960.98
97_A105_A1.9660.98
160_V165_Y1.9420.98
114_T118_G1.9260.98
6_L148_I1.9210.98
262_L265_R1.8990.97
161_L165_Y1.8810.97
239_V243_A1.8170.97
213_H216_D1.7730.96
50_P54_V1.7610.96
98_L106_A1.7330.95
45_L50_P1.6850.95
240_A243_A1.6810.95
32_F35_L1.6740.94
141_N197_D1.6180.93
240_A245_A1.6100.93
5_S74_W1.5990.93
260_V264_K1.5890.93
246_R252_E1.5850.93
240_A244_L1.5630.92
20_E23_Q1.5560.92
140_N145_F1.5550.92
129_L132_V1.5490.92
17_T21_H1.5450.92
155_G161_L1.5050.91
104_E108_E1.4950.90
160_V164_L1.4740.90
113_L116_I1.4500.89
100_R105_A1.4420.89
46_L50_P1.4410.89
207_G266_N1.4230.88
1_M13_E1.4080.87
256_P263_V1.3940.87
10_L13_E1.3790.86
16_F19_G1.3590.85
239_V242_A1.3580.85
55_A59_W1.3550.85
132_V136_L1.3510.85
247_E252_E1.3500.85
247_E250_E1.3480.85
165_Y168_L1.3360.84
260_V263_V1.3360.84
153_V199_I1.3330.84
17_T20_E1.3290.84
106_A110_A1.3200.84
155_G162_Y1.3180.84
30_R34_R1.3160.83
31_Y34_R1.3160.83
16_F23_Q1.3110.83
166_A169_R1.3080.83
30_R33_R1.3070.83
133_Q136_L1.2990.83
110_A154_L1.2970.83
17_T22_W1.2720.81
188_G193_L1.2670.81
233_Y238_R1.2480.80
16_F20_E1.2390.80
90_H93_A1.2370.79
18_L21_H1.2200.78
1_M10_L1.2090.78
39_S45_L1.2060.78
253_V257_R1.2030.77
138_W142_R1.2000.77
161_L166_A1.1990.77
88_R94_Y1.1970.77
110_A113_L1.1900.77
53_L58_L1.1870.77
159_A164_L1.1860.76
41_I45_L1.1810.76
145_F208_Y1.1750.76
81_C160_V1.1750.76
246_R251_D1.1650.75
131_E134_E1.1450.74
28_L31_Y1.1430.74
123_A153_V1.1420.74
271_L274_I1.1300.73
276_L279_L1.1250.73
193_L197_D1.1230.72
158_G221_W1.1150.72
185_A188_G1.1060.71
13_E197_D1.1030.71
57_L191_R1.0970.71
156_P163_R1.0910.70
235_V238_R1.0890.70
81_C86_D1.0880.70
242_A246_R1.0800.69
170_A174_W1.0790.69
120_P124_S1.0700.69
228_F232_N1.0690.69
129_L133_Q1.0680.69
70_Y74_W1.0520.67
128_R132_V1.0520.67
164_L167_L1.0490.67
223_A226_L1.0470.67
65_L72_L1.0420.67
268_L275_A1.0390.66
92_K95_L1.0330.66
38_F41_I1.0290.66
57_L199_I1.0280.66
165_Y169_R1.0260.65
105_A108_E1.0250.65
259_A263_V1.0200.65
238_R242_A1.0200.65
25_D30_R1.0150.65
171_R174_W1.0150.65
162_Y167_L1.0040.64
34_R37_R0.9980.63
46_L51_V0.9950.63
25_D28_L0.9900.63
88_R92_K0.9840.62
7_L12_I0.9810.62
150_W196_L0.9800.62
254_E257_R0.9760.61
205_A217_A0.9750.61
252_E268_L0.9750.61
185_A189_A0.9750.61
16_F22_W0.9590.60
44_L47_A0.9590.60
137_L142_R0.9590.60
44_L49_L0.9590.60
188_G194_H0.9560.60
70_Y160_V0.9510.59
233_Y236_L0.9450.59
24_L27_Y0.9390.58
78_L81_C0.9360.58
238_R241_Q0.9320.58
94_Y140_N0.9250.57
246_R249_H0.9250.57
138_W141_N0.9190.57
278_T281_G0.9170.57
262_L268_L0.9140.56
80_L204_T0.9120.56
45_L51_V0.9100.56
148_I274_I0.9080.56
134_E137_L0.8920.55
135_A138_W0.8890.54
257_R268_L0.8890.54
155_G163_R0.8890.54
87_L210_L0.8810.54
217_A239_V0.8810.54
157_A192_L0.8750.53
241_Q244_L0.8700.53
102_D105_A0.8690.53
139_A144_Y0.8650.52
147_V159_A0.8620.52
7_L39_S0.8590.52
159_A167_L0.8580.52
84_P141_N0.8530.51
232_N240_A0.8510.51
196_L211_V0.8490.51
78_L151_F0.8440.50
204_T210_L0.8420.50
48_L190_D0.8400.50
137_L144_Y0.8360.50
44_L50_P0.8350.50
101_G106_A0.8340.50
130_R134_E0.8340.50
44_L48_L0.8330.50
27_Y31_Y0.8270.49
169_R172_A0.8240.49
110_A114_T0.8210.48
149_F270_W0.8180.48
127_E130_R0.8170.48
243_A246_R0.8170.48
28_L38_F0.8110.48
12_I54_V0.8100.48
256_P264_K0.8090.47
58_L61_L0.8050.47
140_N207_G0.8050.47
100_R108_E0.8030.47
271_L277_L0.7990.47
69_P101_G0.7990.47
190_D194_H0.7960.46
79_L110_A0.7940.46
182_L185_A0.7940.46
112_E118_G0.7910.46
16_F21_H0.7910.46
56_L59_W0.7910.46
136_L144_Y0.7890.46
150_W239_V0.7890.46
83_G150_W0.7880.46
77_V172_A0.7870.46
214_F265_R0.7860.46
272_V281_G0.7800.45
175_L181_P0.7800.45
45_L49_L0.7780.45
133_Q140_N0.7770.45
253_V256_P0.7770.45
192_L195_W0.7760.45
50_P53_L0.7730.44
276_L280_Y0.7730.44
6_L17_T0.7720.44
35_L42_R0.7660.44
46_L49_L0.7640.44
80_L217_A0.7630.44
209_A217_A0.7620.44
81_C84_P0.7480.42
142_R217_A0.7460.42
238_R243_A0.7460.42
4_I10_L0.7420.42
2_T206_L0.7410.42
247_E254_E0.7410.42
84_P91_Y0.7390.42
88_R91_Y0.7330.41
155_G160_V0.7310.41
213_H218_L0.7300.41
54_V62_G0.7290.41
6_L142_R0.7280.41
11_I199_I0.7280.41
51_V55_A0.7250.40
43_T50_P0.7250.40
256_P261_R0.7230.40
194_H208_Y0.7230.40
158_G214_F0.7210.40
254_E266_N0.7200.40
178_R181_P0.7170.40
83_G151_F0.7160.40
263_V270_W0.7150.40
113_L117_H0.7150.40
28_L32_F0.7120.39
188_G191_R0.7120.39
44_L53_L0.7080.39
256_P260_V0.7060.39
40_L53_L0.7050.39
41_I49_L0.7030.39
76_L152_V0.7020.39
168_L173_V0.7020.39
91_Y95_L0.6990.38
54_V221_W0.6990.38
266_N270_W0.6960.38
96_Q100_R0.6880.37
49_L53_L0.6830.37
136_L143_R0.6830.37
89_E92_K0.6820.37
266_N276_L0.6760.37
246_R250_E0.6760.37
94_Y98_L0.6760.37
236_L241_Q0.6740.36
241_Q246_R0.6730.36
13_E204_T0.6710.36
263_V269_I0.6710.36
6_L21_H0.6700.36
265_R270_W0.6700.36
242_A277_L0.6680.36
135_A143_R0.6670.36
33_R38_F0.6640.36
100_R206_L0.6640.36
67_G73_L0.6630.36
74_W151_F0.6590.35
154_L159_A0.6570.35
52_L199_I0.6540.35
236_L243_A0.6540.35
26_R29_D0.6530.35
232_N236_L0.6530.35
90_H160_V0.6500.34
4_I23_Q0.6500.34
157_A161_L0.6450.34
182_L186_Q0.6450.34
141_N150_W0.6430.34
74_W78_L0.6410.34
6_L207_G0.6410.34
237_T244_L0.6400.34
160_V174_W0.6390.34
153_V271_L0.6380.34
211_V269_I0.6380.34
129_L134_E0.6360.33
37_R42_R0.6350.33
51_V66_F0.6340.33
89_E93_A0.6340.33
197_D217_A0.6330.33
274_I281_G0.6330.33
213_H219_P0.6310.33
236_L280_Y0.6310.33
4_I56_L0.6290.33
186_Q190_D0.6290.33
65_L176_A0.6280.33
35_L38_F0.6250.33
84_P90_H0.6240.33
252_E255_T0.6240.33
130_R133_Q0.6220.32
11_I35_L0.6220.32
81_C144_Y0.6210.32
273_V281_G0.6210.32
3_L7_L0.6210.32
259_A266_N0.6190.32
84_P211_V0.6150.32
98_L213_H0.6150.32
91_Y260_V0.6140.32
57_L149_F0.6130.32
15_L18_L0.6090.31
24_L82_L0.6080.31
247_E269_I0.6080.31
28_L34_R0.6070.31
137_L143_R0.6070.31
262_L278_T0.6060.31
218_L260_V0.6050.31
28_L35_L0.6040.31
242_A245_A0.6030.31
87_L195_W0.6030.31
27_Y30_R0.6030.31
84_P87_L0.6020.31
146_A216_D0.6010.31
7_L73_L0.5990.31
209_A216_D0.5970.31
31_Y35_L0.5940.30
33_R36_E0.5930.30
48_L51_V0.5910.30
67_G182_L0.5900.30
83_G136_L0.5880.30
58_L62_G0.5870.30
47_A53_L0.5870.30
137_L140_N0.5860.30
62_G67_G0.5850.30
14_R87_L0.5820.29
14_R17_T0.5810.29
42_R50_P0.5810.29
252_E258_A0.5800.29
72_L118_G0.5790.29
21_H33_R0.5790.29
131_E136_L0.5780.29
41_I53_L0.5780.29
272_V280_Y0.5780.29
253_V274_I0.5770.29
82_L148_I0.5770.29
270_W274_I0.5760.29
141_N259_A0.5750.29
264_K268_L0.5750.29
131_E135_A0.5740.29
149_F259_A0.5730.29
242_A247_E0.5720.29
40_L44_L0.5710.29
167_L170_A0.5690.29
88_R140_N0.5670.28
14_R84_P0.5660.28
93_A97_A0.5650.28
16_F69_P0.5640.28
241_Q245_A0.5630.28
213_H272_V0.5620.28
266_N271_L0.5610.28
181_P188_G0.5600.28
133_Q142_R0.5600.28
86_D91_Y0.5600.28
237_T241_Q0.5560.28
68_L72_L0.5550.28
24_L28_L0.5540.28
63_G156_P0.5530.27
9_A13_E0.5530.27
193_L217_A0.5510.27
139_A207_G0.5500.27
137_L145_F0.5500.27
224_S236_L0.5490.27
270_W273_V0.5490.27
5_S151_F0.5460.27
78_L84_P0.5450.27
118_G227_D0.5450.27
121_A124_S0.5440.27
78_L155_G0.5440.27
84_P197_D0.5440.27
136_L140_N0.5410.27
90_H221_W0.5410.27
6_L152_V0.5400.27
72_L76_L0.5400.27
239_V245_A0.5400.27
124_S136_L0.5370.26
267_L276_L0.5340.26
133_Q144_Y0.5340.26
56_L187_S0.5340.26
249_H252_E0.5330.26
197_D211_V0.5300.26
41_I65_L0.5280.26
69_P72_L0.5270.26
91_Y94_Y0.5250.26
81_C85_R0.5230.25
233_Y239_V0.5220.25
82_L145_F0.5210.25
82_L147_V0.5200.25
128_R131_E0.5200.25
37_R41_I0.5190.25
125_E129_L0.5190.25
265_R278_T0.5180.25
13_E133_Q0.5170.25
228_F233_Y0.5160.25
256_P269_I0.5100.25
157_A162_Y0.5100.25
105_A110_A0.5090.25
76_L125_E0.5080.25
13_E97_A0.5070.24
234_Q238_R0.5050.24
185_A194_H0.5050.24
14_R88_R0.5050.24
141_N163_R0.5040.24
224_S249_H0.5020.24
254_E258_A0.5020.24
68_L186_Q0.5010.24
123_A229_H0.5010.24
235_V239_V0.5000.24
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1xnfA 2 0.3099 12.2 0.965 Contact Map
2mn6B 2 0.1408 11.8 0.965 Contact Map
1qleD 1 0.1514 10.8 0.966 Contact Map
4a1sA 1 0.3028 10.4 0.966 Contact Map
2l16A 1 0.1585 9.8 0.966 Contact Map
2jlnA 1 0.3556 9.6 0.966 Contact Map
2vsyA 1 0.2923 6 0.969 Contact Map
2lzrA 1 0.1725 5.4 0.97 Contact Map
3gw4A 2 0.3838 4.6 0.971 Contact Map
4pypA 1 0.2993 4.5 0.971 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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