GREMLIN Database
DUF5009 - Domain of unknown function (DUF5009)
PFAM: PF16401 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 260 (211)
Sequences: 1202 (997)
Seq/√Len: 68.6
META: 0.892

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
185_I188_A3.3101.00
74_R78_K2.9291.00
153_G157_L2.8471.00
183_G186_G2.7741.00
175_T178_W2.7661.00
129_L132_L2.7111.00
93_T189_L2.6361.00
52_F56_V2.6271.00
174_L178_W2.6231.00
34_A49_G2.4771.00
74_R77_E2.4261.00
176_R180_G2.3391.00
157_L161_A2.2911.00
180_G183_G2.2621.00
181_I184_L2.2081.00
174_L177_S2.1931.00
95_A99_F2.1371.00
150_R153_G2.0681.00
81_S84_K2.0471.00
22_L53_V2.0421.00
127_F130_L2.0331.00
176_R179_I2.0041.00
131_F134_W1.9851.00
177_S181_I1.9731.00
183_G187_W1.8571.00
184_L188_A1.8291.00
86_L193_L1.7871.00
27_L32_Y1.7591.00
11_L15_L1.7531.00
177_S180_G1.7201.00
93_T97_L1.7011.00
157_L160_L1.6400.99
184_L187_W1.6340.99
180_G184_L1.6250.99
129_L156_L1.6090.99
31_M53_V1.6060.99
80_D84_K1.5670.99
131_F135_N1.5670.99
122_L126_G1.5670.99
155_L159_L1.5640.99
178_W181_I1.5580.99
201_N204_L1.5320.99
10_G13_I1.4940.99
75_R88_H1.4920.99
176_R181_I1.4780.99
212_L215_L1.4570.99
172_F179_I1.4500.98
127_F131_F1.4490.98
180_G185_I1.4470.98
163_I166_Y1.4410.98
122_L134_W1.4410.98
34_A48_D1.4400.98
159_L163_I1.4300.98
122_L129_L1.4170.98
118_S122_L1.3980.98
53_V56_V1.3720.98
129_L134_W1.3710.98
131_F137_Y1.3320.97
179_I183_G1.3310.97
194_I208_V1.3200.97
182_L185_I1.3200.97
124_L128_I1.3100.97
5_I66_M1.3060.97
111_L114_A1.3000.97
151_I155_L1.2900.97
147_L151_I1.2900.97
84_K87_L1.2820.97
149_L153_G1.2740.97
122_L127_F1.2660.96
187_W190_F1.2630.96
76_L85_I1.2550.96
145_L148_I1.2510.96
148_I151_I1.2470.96
76_L82_K1.2470.96
185_I189_L1.2460.96
132_L137_Y1.2460.96
5_I67_S1.2330.96
181_I185_I1.2320.96
126_G129_L1.2280.96
52_F55_L1.2220.96
103_M127_F1.2190.96
130_L134_W1.2160.95
15_L52_F1.2040.95
142_P146_F1.1960.95
110_V115_P1.1770.95
162_L166_Y1.1730.95
173_S178_W1.1700.94
161_A164_Y1.1670.94
149_L157_L1.1460.94
111_L115_P1.1340.93
117_L122_L1.1330.93
82_K199_R1.1320.93
145_L149_L1.1260.93
179_I182_L1.1100.93
183_G188_A1.1020.92
144_K148_I1.0980.92
212_L216_F1.0970.92
30_W33_H1.0890.92
91_L95_A1.0880.92
6_D9_R1.0850.92
216_F219_A1.0820.92
122_L131_F1.0760.92
172_F177_S1.0760.92
157_L166_Y1.0370.90
70_F74_R1.0340.90
157_L165_R1.0300.90
115_P118_S1.0290.90
75_R80_D1.0230.89
121_L125_L1.0220.89
132_L135_N1.0110.89
204_L208_V1.0100.89
174_L179_I1.0010.88
173_S176_R0.9890.88
213_L216_F0.9830.87
205_R208_V0.9800.87
175_T179_I0.9780.87
89_I192_S0.9760.87
179_I184_L0.9730.87
7_A11_L0.9710.87
13_I53_V0.9700.87
83_W87_L0.9620.86
156_L159_L0.9530.86
152_A159_L0.9510.86
186_G189_L0.9510.86
153_G156_L0.9510.86
182_L186_G0.9450.85
31_M52_F0.9400.85
128_I131_F0.9390.85
180_G186_G0.9300.84
198_T204_L0.9270.84
13_I66_M0.9240.84
122_L132_L0.9210.84
28_P33_H0.9200.84
111_L116_G0.9090.83
45_P48_D0.9040.83
5_I13_I0.9040.83
172_F180_G0.9030.82
134_W137_Y0.8970.82
207_G214_A0.8920.82
35_Q49_G0.8910.82
32_Y53_V0.8900.82
161_A165_R0.8830.81
13_I51_T0.8820.81
99_F130_L0.8800.81
178_W184_L0.8670.80
94_L144_K0.8630.80
147_L155_L0.8630.80
150_R158_I0.8570.79
87_L90_L0.8450.78
85_I206_L0.8450.78
209_L216_F0.8370.77
125_L129_L0.8310.77
173_S179_I0.8300.77
163_I167_R0.8180.76
203_L206_L0.8160.76
22_L56_V0.8150.76
161_A166_Y0.8130.75
161_A167_R0.8080.75
143_K148_I0.7990.74
201_N205_R0.7940.74
130_L137_Y0.7830.73
68_I196_L0.7780.72
172_F178_W0.7710.71
210_A213_L0.7670.71
48_D51_T0.7610.70
162_L165_R0.7580.70
172_F181_I0.7560.70
80_D85_I0.7490.69
130_L133_I0.7480.69
179_I187_W0.7480.69
111_L118_S0.7470.69
82_K86_L0.7470.69
13_I23_A0.7460.69
3_L11_L0.7430.69
165_R168_D0.7430.69
4_S8_L0.7430.69
30_W53_V0.7430.69
134_W138_P0.7390.68
58_P65_G0.7360.68
173_S177_S0.7360.68
10_G53_V0.7350.68
7_A12_T0.7350.68
104_V107_E0.7330.68
95_A134_W0.7320.67
115_P119_K0.7280.67
123_A126_G0.7280.67
97_L182_L0.7270.67
114_A118_S0.7260.67
27_L53_V0.7130.65
198_T202_L0.7120.65
126_G132_L0.7100.65
160_L165_R0.7100.65
150_R157_L0.7090.65
195_Y205_R0.7070.65
210_A214_A0.7070.65
87_L91_L0.7020.64
179_I185_I0.7010.64
71_A75_R0.7000.64
32_Y52_F0.6990.64
131_F136_R0.6980.64
56_V101_V0.6950.63
211_F217_L0.6910.63
150_R155_L0.6890.63
138_P146_F0.6890.63
122_L125_L0.6880.63
123_A127_F0.6880.63
29_A53_V0.6860.62
190_F208_V0.6860.62
198_T208_V0.6850.62
5_I70_F0.6850.62
133_I137_Y0.6840.62
175_T180_G0.6830.62
129_L133_I0.6830.62
124_L133_I0.6810.62
18_F22_L0.6760.61
97_L104_V0.6760.61
86_L196_L0.6760.61
127_F134_W0.6750.61
116_G119_K0.6730.61
127_F136_R0.6700.61
126_G160_L0.6680.60
205_R209_L0.6670.60
119_K123_A0.6660.60
11_L49_G0.6620.60
174_L181_I0.6580.59
10_G99_F0.6580.59
112_S115_P0.6540.59
22_L34_A0.6530.59
72_I195_Y0.6520.59
157_L164_Y0.6510.58
119_K122_L0.6500.58
148_I152_A0.6490.58
15_L53_V0.6480.58
8_L12_T0.6460.58
76_L124_L0.6440.58
13_I21_D0.6410.57
8_L59_A0.6370.57
23_A32_Y0.6360.57
173_S180_G0.6340.56
2_I5_I0.6330.56
21_D24_G0.6260.55
188_A192_S0.6260.55
12_T30_W0.6200.55
17_I21_D0.6190.55
172_F176_R0.6180.54
20_N183_G0.6180.54
32_Y35_Q0.6130.54
89_I196_L0.6120.54
209_L212_L0.6080.53
181_I186_G0.6080.53
24_G32_Y0.6000.52
113_A116_G0.6000.52
128_I132_L0.5980.52
142_P145_L0.5960.52
113_A118_S0.5940.52
22_L32_Y0.5930.52
91_L98_V0.5930.52
25_K32_Y0.5930.52
12_T19_V0.5920.51
189_L193_L0.5900.51
124_L127_F0.5900.51
106_A126_G0.5890.51
210_A216_F0.5890.51
149_L152_A0.5880.51
101_V105_N0.5830.50
87_L154_I0.5820.50
12_T20_N0.5770.50
138_P142_P0.5760.49
29_A32_Y0.5740.49
103_M126_G0.5740.49
8_L31_M0.5730.49
33_H57_F0.5730.49
132_L153_G0.5720.49
126_G134_W0.5720.49
7_A14_L0.5720.49
54_D60_F0.5720.49
78_K84_K0.5710.49
13_I52_F0.5700.49
34_A47_V0.5700.49
22_L27_L0.5650.48
34_A46_D0.5650.48
102_F157_L0.5640.48
17_I103_M0.5610.48
72_I196_L0.5600.48
167_R171_G0.5600.48
65_G71_A0.5600.48
48_D52_F0.5600.48
136_R145_L0.5570.47
20_N63_I0.5570.47
55_L59_A0.5510.46
10_G55_L0.5510.46
85_I196_L0.5510.46
64_V68_I0.5490.46
16_M28_P0.5460.46
174_L180_G0.5450.46
147_L156_L0.5440.46
111_L122_L0.5410.45
210_A217_L0.5410.45
10_G51_T0.5400.45
193_L197_F0.5400.45
213_L217_L0.5390.45
61_L187_W0.5360.45
153_G164_Y0.5330.44
170_E173_S0.5320.44
194_I215_L0.5320.44
64_V185_I0.5310.44
51_T56_V0.5310.44
120_W127_F0.5290.44
144_K147_L0.5290.44
142_P147_L0.5280.44
211_F214_A0.5260.43
6_D10_G0.5170.42
23_A51_T0.5140.42
14_L218_A0.5000.40
126_G130_L0.5000.40
15_L56_V0.5000.40
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2mkvA 1 0.2423 34.1 0.959 Contact Map
3wguG 1 0.1269 26.7 0.961 Contact Map
2jo1A 1 0.2462 22 0.962 Contact Map
2zxeG 1 0.15 7.2 0.97 Contact Map
3l32A 2 0.1385 6 0.971 Contact Map
2jp3A 1 0.2577 5.2 0.972 Contact Map
4f35D 2 0.3577 3.6 0.974 Contact Map
2mgyA 1 0.6115 3.3 0.974 Contact Map
4ryoA 1 0.5615 2.1 0.977 Contact Map
2nq2A 2 0.6692 2.1 0.977 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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