GREMLIN Database
DUF4869 - Domain of unknown function (DUF4869)
PFAM: PF16163 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 128 (126)
Sequences: 551 (373)
Seq/√Len: 33.2
META: 0.768

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
39_L47_P3.9951.00
27_K33_E3.9231.00
86_K89_K3.5621.00
63_M84_I3.5371.00
63_M93_I3.1261.00
22_V61_I2.8391.00
24_E86_K2.7561.00
25_M57_V2.7471.00
28_D82_K2.5681.00
3_N6_V2.4821.00
28_D83_W2.3940.99
45_L48_I2.2090.99
56_G79_N2.1780.99
4_T75_T2.1570.99
20_P23_K2.1520.99
109_E120_H2.1210.99
111_K116_G2.0850.98
84_I93_I2.0270.98
49_P52_E1.9070.97
33_E41_D1.8820.97
32_S40_I1.8690.97
120_H124_E1.8620.97
122_M125_L1.7360.95
112_I125_L1.6990.94
25_M61_I1.6960.94
35_I39_L1.6190.93
22_V65_N1.6100.93
67_P91_K1.5810.92
59_T76_C1.4310.87
112_I119_V1.4260.87
32_S42_S1.4120.87
40_I53_L1.3560.84
53_L57_V1.3180.83
59_T71_F1.3010.82
73_A95_I1.2920.81
54_S57_V1.2790.81
20_P24_E1.2720.80
84_I95_I1.2680.80
18_T23_K1.2540.79
21_L64_Y1.2480.79
19_D64_Y1.2460.79
109_E121_N1.2230.78
84_I110_I1.2210.77
22_V64_Y1.2110.77
21_L25_M1.1970.76
23_K33_E1.1360.72
78_D99_H1.0940.69
97_L101_M1.0810.68
38_H52_E1.0770.68
34_V40_I1.0610.67
50_P53_L1.0400.65
42_S48_I1.0320.65
60_L87_I1.0280.64
121_N124_E1.0260.64
21_L87_I1.0150.63
21_L82_K0.9860.61
29_V57_V0.9730.60
66_D69_K0.9720.60
25_M87_I0.9620.59
61_I65_N0.9530.58
92_D109_E0.9380.57
42_S55_G0.9350.57
110_I118_I0.9300.56
89_K109_E0.9270.56
32_S44_V0.9260.56
38_H84_I0.9140.55
38_H41_D0.8940.53
63_M73_A0.8850.53
40_I82_K0.8810.52
81_A97_L0.8750.52
104_G122_M0.8720.52
19_D22_V0.8690.51
32_S43_P0.8590.51
59_T63_M0.8580.50
81_A101_M0.8500.50
115_T126_V0.8480.50
24_E27_K0.8380.49
61_I75_T0.8330.48
115_T121_N0.8300.48
87_I93_I0.8200.47
85_L110_I0.8070.46
72_N114_N0.8030.46
110_I119_V0.8010.46
117_K120_H0.7930.45
25_M83_W0.7920.45
12_Y16_W0.7910.45
5_S52_E0.7870.45
28_D57_V0.7850.44
16_W80_C0.7780.44
107_P121_N0.7700.43
18_T22_V0.7530.42
6_V10_N0.7430.41
5_S47_P0.7430.41
6_V20_P0.7410.41
57_V83_W0.7400.41
17_I22_V0.7290.40
14_D18_T0.7280.40
63_M67_P0.7270.40
104_G111_K0.7270.40
36_G39_L0.7200.39
2_Y99_H0.7170.39
5_S9_K0.7160.39
96_N99_H0.7130.39
60_L80_C0.7080.38
68_D125_L0.7040.38
42_S45_L0.7000.37
39_L49_P0.6880.37
13_E44_V0.6830.36
11_T22_V0.6820.36
21_L116_G0.6710.35
85_L89_K0.6670.35
61_I84_I0.6640.35
25_M38_H0.6580.34
6_V65_N0.6560.34
42_S46_G0.6560.34
30_D77_G0.6520.34
17_I26_I0.6510.34
64_Y87_I0.6500.34
9_K51_E0.6490.34
22_V87_I0.6480.34
72_N99_H0.6430.33
94_T113_L0.6410.33
109_E118_I0.6410.33
4_T11_T0.6290.32
52_E68_D0.6270.32
117_K121_N0.6210.32
67_P92_D0.6210.32
62_L75_T0.6190.31
31_K116_G0.6110.31
59_T119_V0.6100.31
5_S75_T0.6090.31
14_D37_P0.6040.30
43_P46_G0.6040.30
67_P118_I0.6030.30
64_Y91_K0.6030.30
108_F112_I0.5990.30
22_V25_M0.5940.30
42_S73_A0.5940.30
4_T62_L0.5930.30
122_M126_V0.5910.29
38_H51_E0.5880.29
104_G125_L0.5870.29
26_I40_I0.5830.29
34_V50_P0.5810.29
76_C97_L0.5750.28
111_K118_I0.5740.28
100_L126_V0.5690.28
25_M29_V0.5670.28
86_K90_K0.5650.28
117_K122_M0.5650.28
24_E36_G0.5640.28
7_Y66_D0.5640.28
7_Y69_K0.5640.28
115_T120_H0.5630.28
55_G80_C0.5610.27
87_I103_F0.5610.27
13_E64_Y0.5590.27
36_G47_P0.5570.27
65_N91_K0.5560.27
23_K27_K0.5550.27
85_L100_L0.5530.27
93_I112_I0.5530.27
63_M71_F0.5530.27
117_K125_L0.5520.27
39_L82_K0.5470.26
47_P50_P0.5450.26
73_A81_A0.5400.26
33_E68_D0.5380.26
57_V61_I0.5370.26
71_F74_S0.5360.26
28_D88_A0.5340.26
59_T70_I0.5330.26
40_I50_P0.5320.25
123_K126_V0.5270.25
31_K44_V0.5270.25
72_N78_D0.5250.25
30_D79_N0.5210.25
43_P57_V0.5210.25
40_I73_A0.5210.25
5_S100_L0.5200.25
18_T31_K0.5180.25
69_K117_K0.5160.24
97_L103_F0.5140.24
30_D54_S0.5130.24
3_N9_K0.5120.24
11_T65_N0.5110.24
15_E41_D0.5070.24
35_I47_P0.5010.24
100_L125_L0.5000.24
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4ry2A 2 0.9688 56.1 0.917 Contact Map
4a82A 2 0.9688 32.4 0.928 Contact Map
4pl0A 2 0.9688 31.7 0.928 Contact Map
4q9hA 1 0.9609 30.1 0.929 Contact Map
4q4hB 1 0.9688 29.5 0.93 Contact Map
4f4cA 1 0.9688 28.7 0.93 Contact Map
3b60A 2 0.9688 21.4 0.934 Contact Map
4mrsA 2 0.9688 20.6 0.935 Contact Map
3nh6A 1 0.9688 19.2 0.936 Contact Map
2ghiA 1 0.9688 18.5 0.936 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0167 seconds.