GREMLIN Database
DUF4856 - Domain of unknown function (DUF4856)
PFAM: PF16148 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 358 (353)
Sequences: 790 (667)
Seq/√Len: 35.5
META: 0.777

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
261_E264_K4.2911.00
247_D250_V4.2461.00
231_D261_E4.0671.00
153_I262_L3.9971.00
150_Y262_L3.9661.00
167_T171_D3.8181.00
113_S116_V3.7221.00
40_E44_L3.5691.00
230_D234_D3.5561.00
254_Q257_I3.3781.00
69_R94_D3.1781.00
67_Q70_S2.9611.00
175_G246_K2.8761.00
93_F143_G2.8231.00
178_V185_T2.8201.00
318_K322_D2.8141.00
241_A245_A2.7701.00
187_E244_V2.7491.00
29_L140_F2.7401.00
49_N55_P2.6631.00
275_Q348_S2.5381.00
135_E291_H2.3841.00
86_S90_K2.3741.00
66_K71_K2.3430.99
297_Y325_L2.3310.99
145_I212_L2.3170.99
46_M49_N2.3100.99
320_E354_K2.3070.99
198_Y208_N2.2880.99
85_E304_Q2.2870.99
130_N136_Y2.2470.99
177_L182_N2.1780.99
220_N227_G2.1190.99
94_D97_I2.1030.99
199_G203_D2.0980.99
186_M251_R2.0590.99
301_Y305_F2.0120.98
18_G192_E2.0030.98
317_S320_E1.9430.98
250_V253_E1.9330.98
171_D178_V1.9230.98
170_A174_N1.9100.98
190_W239_G1.9080.98
231_D264_K1.9050.98
41_A45_A1.8980.98
59_A63_A1.8950.98
278_K345_D1.8340.97
134_L139_A1.8100.97
52_G55_P1.8070.97
12_S182_N1.7970.97
38_L42_K1.7800.96
99_D103_E1.7750.96
295_E299_F1.7370.96
22_R192_E1.7320.96
49_N52_G1.7220.96
214_K296_G1.7130.96
45_A49_N1.6870.95
30_I141_E1.6870.95
45_A51_N1.6830.95
78_Y314_P1.6820.95
197_L216_L1.6660.95
112_A120_I1.6410.94
16_F194_F1.6230.94
56_F61_L1.6180.94
18_G157_Y1.6020.94
238_L250_V1.5440.92
18_G22_R1.5180.92
316_F324_F1.5110.92
51_N55_P1.4920.91
200_A213_N1.4890.91
139_A334_F1.4850.91
171_D176_T1.4830.91
162_V189_K1.4730.90
24_A200_A1.4710.90
212_L266_I1.4650.90
233_Y237_K1.4370.89
316_F354_K1.4170.89
159_S259_R1.4110.88
33_L140_F1.3910.88
45_A55_P1.3810.87
220_N226_A1.3800.87
170_A173_D1.3760.87
353_A356_G1.3690.87
156_N162_V1.3680.87
276_Q292_A1.3540.86
68_L148_L1.3520.86
40_E98_T1.3330.85
263_S306_T1.3270.85
39_T42_K1.3260.85
19_Q192_E1.3250.85
19_Q157_Y1.3240.85
316_F320_E1.3220.85
27_E210_S1.3160.85
23_L149_M1.3080.84
236_F258_I1.3070.84
120_I287_A1.3010.84
120_I129_V1.2990.84
167_T170_A1.2780.83
323_A326_A1.2560.82
154_L262_L1.2540.82
350_A354_K1.2410.81
201_P204_P1.2380.81
275_Q278_K1.2330.80
346_A350_A1.2310.80
306_T315_Y1.2180.80
138_Q299_F1.2150.79
264_K268_A1.2090.79
200_A203_D1.2080.79
76_S165_A1.2080.79
2_A237_K1.1990.79
178_V181_K1.1950.78
157_Y192_E1.1920.78
120_I286_Y1.1910.78
68_L149_M1.1860.78
203_D206_N1.1860.78
56_F62_N1.1820.78
72_T148_L1.1740.77
186_M252_D1.1710.77
68_L72_T1.1680.77
11_E182_N1.1660.77
48_A69_R1.1650.77
194_F207_P1.1640.76
99_D136_Y1.1630.76
322_D325_L1.1490.76
225_F357_F1.1490.76
343_T347_I1.1390.75
119_Q126_T1.1390.75
44_L94_D1.1380.75
250_V254_Q1.1340.75
92_D301_Y1.1330.74
13_T182_N1.1290.74
26_A141_E1.1270.74
26_A29_L1.1270.74
50_E55_P1.1260.74
16_F20_T1.1080.73
304_Q322_D1.1060.73
260_E264_K1.1040.73
327_Q331_G1.1010.72
154_L263_S1.0960.72
122_D285_D1.0960.72
40_E46_M1.0950.72
322_D326_A1.0940.72
331_G347_I1.0930.72
273_Y295_E1.0860.71
248_Y251_R1.0830.71
25_M61_L1.0830.71
24_A58_D1.0800.71
90_K97_I1.0720.70
321_V325_L1.0690.70
87_A91_A1.0640.70
43_L46_M1.0630.70
200_A267_A1.0590.69
337_V343_T1.0580.69
134_L297_Y1.0570.69
291_H299_F1.0560.69
16_F19_Q1.0560.69
19_Q152_Q1.0520.69
337_V344_L1.0500.69
332_N336_D1.0490.69
327_Q330_A1.0470.68
214_K292_A1.0430.68
173_D176_T1.0400.68
122_D287_A1.0360.68
32_A35_D1.0330.67
96_W140_F1.0250.67
56_F59_A1.0130.66
119_Q287_A1.0070.65
349_E352_A1.0020.65
19_Q188_H1.0010.65
164_D186_M0.9990.65
220_N229_A0.9930.64
47_F52_G0.9920.64
157_Y188_H0.9920.64
134_L334_F0.9850.64
146_G302_S0.9830.63
331_G336_D0.9820.63
194_F216_L0.9810.63
273_Y291_H0.9800.63
139_A297_Y0.9720.63
340_T343_T0.9710.62
327_Q350_A0.9680.62
163_L255_A0.9670.62
151_D155_N0.9620.62
164_D251_R0.9620.62
24_A210_S0.9590.62
219_V269_R0.9550.61
200_A204_P0.9540.61
121_A124_S0.9530.61
330_A334_F0.9460.60
256_A310_N0.9410.60
255_A258_I0.9360.60
160_T164_D0.9340.59
57_S60_D0.9310.59
242_A247_D0.9290.59
329_M337_V0.9190.58
39_T49_N0.9140.58
328_L347_I0.9140.58
19_Q22_R0.9140.58
202_A209_L0.9130.58
20_T24_A0.9130.58
165_A189_K0.9120.58
1_P4_Y0.9070.57
343_T348_S0.9050.57
211_F301_Y0.9050.57
291_H295_E0.9020.57
184_T195_G0.8990.57
248_Y252_D0.8930.56
327_Q346_A0.8920.56
29_L68_L0.8920.56
55_P62_N0.8850.55
274_L300_I0.8840.55
169_R173_D0.8780.55
214_K218_R0.8740.54
222_D225_F0.8700.54
268_A277_G0.8700.54
278_K348_S0.8670.54
203_D209_L0.8610.53
112_A118_G0.8610.53
153_I177_L0.8570.53
48_A55_P0.8550.53
153_I197_L0.8530.53
327_Q347_I0.8500.52
31_S34_K0.8470.52
234_D237_K0.8460.52
29_L43_L0.8440.52
137_N141_E0.8430.52
171_D185_T0.8430.52
77_D159_S0.8410.52
71_K74_A0.8400.51
208_N216_L0.8400.51
163_L189_K0.8400.51
201_P208_N0.8380.51
238_L245_A0.8360.51
13_T183_Y0.8360.51
92_D96_W0.8350.51
342_A346_A0.8320.51
60_D63_A0.8310.51
132_K331_G0.8300.51
208_N217_N0.8270.50
235_A254_Q0.8210.50
40_E102_A0.8200.50
43_L47_F0.8200.50
45_A48_A0.8180.50
149_M298_G0.8170.49
59_A62_N0.8100.49
156_N163_L0.8090.49
151_D156_N0.8080.49
18_G188_H0.8080.49
127_R269_R0.8040.48
111_T119_Q0.8030.48
245_A250_V0.8020.48
160_T259_R0.8020.48
3_T6_F0.8010.48
293_L297_Y0.8000.48
38_L49_N0.7950.48
22_R157_Y0.7940.47
324_F350_A0.7940.47
194_F305_F0.7930.47
58_D61_L0.7930.47
274_L278_K0.7910.47
349_E353_A0.7860.47
18_G66_K0.7850.47
256_A259_R0.7840.47
238_L241_A0.7830.47
269_R275_Q0.7820.46
272_Y276_Q0.7740.46
16_F265_V0.7730.46
264_K356_G0.7710.45
41_A44_L0.7700.45
138_Q303_L0.7700.45
304_Q321_V0.7690.45
163_L251_R0.7670.45
138_Q144_L0.7670.45
218_R272_Y0.7660.45
211_F296_G0.7640.45
71_K192_E0.7640.45
56_F63_A0.7610.45
44_L48_A0.7600.45
276_Q282_A0.7590.44
255_A259_R0.7580.44
216_L232_I0.7570.44
150_Y266_I0.7570.44
57_S62_N0.7560.44
203_D207_P0.7550.44
33_L96_W0.7540.44
335_W351_I0.7530.44
198_Y306_T0.7510.44
25_M63_A0.7490.44
273_Y299_F0.7490.44
134_L300_I0.7450.43
249_T253_E0.7420.43
139_A298_G0.7400.43
58_D63_A0.7380.43
238_L254_Q0.7370.43
333_G336_D0.7330.42
57_S246_K0.7310.42
117_A304_Q0.7300.42
141_E211_F0.7290.42
18_G71_K0.7260.42
175_G178_V0.7250.42
38_L46_M0.7190.41
25_M55_P0.7180.41
29_L250_V0.7170.41
173_D246_K0.7170.41
203_D234_D0.7150.41
218_R292_A0.7150.41
226_A267_A0.7140.41
232_I262_L0.7130.41
230_D313_A0.7120.40
262_L265_V0.7120.40
167_T174_N0.7110.40
300_I314_P0.7100.40
33_L97_I0.7090.40
135_E143_G0.7080.40
135_E295_E0.7070.40
17_S21_T0.7020.40
158_L254_Q0.7000.40
235_A257_I0.6980.39
324_F347_I0.6960.39
135_E273_Y0.6950.39
205_A208_N0.6940.39
342_A356_G0.6940.39
304_Q315_Y0.6930.39
1_P6_F0.6930.39
309_P314_P0.6900.39
198_Y203_D0.6890.39
33_L38_L0.6860.38
69_R86_S0.6860.38
142_K295_E0.6830.38
10_G13_T0.6830.38
201_P206_N0.6820.38
49_N65_G0.6820.38
196_Y199_G0.6800.38
238_L253_E0.6800.38
124_S285_D0.6790.38
331_G334_F0.6790.38
269_R272_Y0.6780.38
270_A300_I0.6750.37
347_I350_A0.6750.37
345_D348_S0.6740.37
114_A332_N0.6730.37
176_T319_A0.6730.37
193_A301_Y0.6720.37
199_G204_P0.6720.37
111_T121_A0.6710.37
186_M248_Y0.6700.37
236_F256_A0.6680.37
272_Y275_Q0.6670.37
36_T108_W0.6650.37
61_L64_S0.6650.37
48_A51_N0.6640.37
142_K299_F0.6640.37
217_N221_G0.6630.36
23_L77_D0.6630.36
194_F233_Y0.6630.36
306_T309_P0.6620.36
39_T46_M0.6620.36
304_Q318_K0.6610.36
129_V136_Y0.6600.36
297_Y334_F0.6600.36
66_K157_Y0.6600.36
320_E325_L0.6600.36
194_F204_P0.6550.36
104_V107_N0.6530.36
346_A349_E0.6500.35
134_L329_M0.6470.35
296_G300_I0.6440.35
232_I258_I0.6430.35
16_F216_L0.6420.35
122_D127_R0.6410.35
107_N110_T0.6410.35
114_A317_S0.6360.34
1_P13_T0.6360.34
297_Y329_M0.6360.34
189_K193_A0.6350.34
345_D349_E0.6340.34
229_A233_Y0.6320.34
117_A174_N0.6320.34
326_A329_M0.6320.34
193_A211_F0.6300.34
69_R90_K0.6280.34
201_P209_L0.6280.34
166_G171_D0.6280.34
114_A333_G0.6260.34
268_A292_A0.6250.34
40_E47_F0.6250.34
26_A68_L0.6250.34
46_M61_L0.6240.33
215_Y295_E0.6240.33
40_E49_N0.6240.33
214_K268_A0.6230.33
258_I266_I0.6230.33
120_I301_Y0.6200.33
110_T116_V0.6190.33
319_A342_A0.6190.33
75_S151_D0.6180.33
171_D177_L0.6180.33
153_I212_L0.6170.33
219_V290_F0.6170.33
23_L28_E0.6160.33
137_N298_G0.6160.33
202_A207_P0.6150.33
55_P61_L0.6140.33
308_N313_A0.6120.33
153_I254_Q0.6110.32
47_F72_T0.6110.32
329_M333_G0.6110.32
231_D234_D0.6100.32
243_I251_R0.6100.32
218_R221_G0.6080.32
20_T199_G0.6070.32
228_I265_V0.6070.32
352_A357_F0.6060.32
68_L296_G0.6060.32
97_I124_S0.6050.32
121_A287_A0.6040.32
123_G270_A0.6010.32
280_A292_A0.6000.32
76_S80_S0.5990.32
285_D288_D0.5980.31
257_I261_E0.5960.31
138_Q141_E0.5960.31
326_A346_A0.5950.31
98_T102_A0.5940.31
318_K321_V0.5930.31
99_D356_G0.5910.31
32_A46_M0.5910.31
51_N54_N0.5880.31
66_K70_S0.5880.31
114_A336_D0.5860.31
112_A129_V0.5840.30
260_E315_Y0.5830.30
19_Q66_K0.5830.30
297_Y347_I0.5810.30
113_S166_G0.5810.30
15_S181_K0.5800.30
159_S300_I0.5790.30
119_Q122_D0.5780.30
45_A52_G0.5760.30
103_E106_P0.5750.30
180_G346_A0.5740.30
71_K157_Y0.5720.30
201_P207_P0.5710.29
194_F258_I0.5710.29
277_G281_L0.5700.29
300_I321_V0.5690.29
205_A209_L0.5690.29
178_V183_Y0.5680.29
53_A68_L0.5660.29
140_F297_Y0.5650.29
48_A52_G0.5650.29
266_I302_S0.5640.29
225_F228_I0.5610.29
317_S354_K0.5610.29
237_K241_A0.5600.29
150_Y154_L0.5600.29
291_H296_G0.5580.29
135_E214_K0.5580.29
351_I354_K0.5570.28
44_L69_R0.5560.28
86_S94_D0.5540.28
18_G196_Y0.5530.28
262_L289_A0.5520.28
304_Q309_P0.5510.28
72_T144_L0.5500.28
174_N177_L0.5500.28
60_D78_Y0.5490.28
197_L232_I0.5490.28
14_V18_G0.5490.28
342_A345_D0.5480.28
152_Q157_Y0.5480.28
76_S83_T0.5470.28
48_A59_A0.5460.28
47_F51_N0.5440.28
137_N148_L0.5440.28
112_A155_N0.5440.28
53_A56_F0.5430.28
98_T317_S0.5430.28
32_A36_T0.5420.27
328_L333_G0.5410.27
11_E179_E0.5410.27
299_F303_L0.5410.27
22_R273_Y0.5400.27
212_L229_A0.5400.27
54_N246_K0.5390.27
73_A254_Q0.5390.27
69_R91_A0.5390.27
18_G152_Q0.5370.27
39_T48_A0.5370.27
306_T321_V0.5350.27
317_S334_F0.5350.27
79_F82_N0.5350.27
166_G314_P0.5340.27
2_A230_D0.5330.27
319_A330_A0.5320.27
7_E14_V0.5310.27
121_A281_L0.5310.27
349_E356_G0.5300.27
315_Y318_K0.5300.27
45_A50_E0.5280.26
330_A343_T0.5280.26
4_Y8_R0.5270.26
38_L50_E0.5260.26
165_A168_N0.5250.26
33_L49_N0.5240.26
201_P233_Y0.5240.26
173_D182_N0.5230.26
153_I236_F0.5230.26
334_F354_K0.5210.26
163_L186_M0.5200.26
307_R310_N0.5200.26
144_L187_E0.5200.26
142_K215_Y0.5190.26
127_R225_F0.5190.26
158_L258_I0.5190.26
141_E150_Y0.5180.26
194_F201_P0.5180.26
300_I319_A0.5160.26
92_D143_G0.5160.26
137_N216_L0.5150.26
328_L336_D0.5140.26
105_F108_W0.5130.25
7_E12_S0.5090.25
85_E318_K0.5080.25
8_R244_V0.5070.25
315_Y321_V0.5060.25
26_A148_L0.5060.25
9_N13_T0.5030.25
191_D240_R0.5000.25
45_A62_N0.5000.25
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4fzpA 2 0.2263 24.3 0.973 Contact Map
3pzpA 1 0.1536 17 0.975 Contact Map
3hxaA 5 0.1061 10.1 0.977 Contact Map
1dd4C 7 0.0894 8.3 0.978 Contact Map
4wilA 4 0.1061 6.5 0.979 Contact Map
2oo2A 2 0.2123 6.4 0.979 Contact Map
4f3lB 1 0.2933 5.3 0.98 Contact Map
2hiyA 2 0.1732 5 0.98 Contact Map
3dr4A 2 0.1369 4.7 0.98 Contact Map
2v6uA 4 0.1117 4.4 0.981 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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