GREMLIN Database
DUF4835 - Domain of unknown function (DUF4835)
PFAM: PF16119 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 277 (275)
Sequences: 4732 (3228)
Seq/√Len: 194.7
META: 0.902

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
59_E67_K4.2411.00
87_L134_S3.6771.00
33_M124_A3.5661.00
29_L120_L3.3031.00
205_N208_E3.0671.00
41_D132_Y2.9761.00
214_L248_F2.9281.00
105_L117_T2.6651.00
33_M120_L2.5911.00
95_F119_V2.5481.00
5_C53_F2.5171.00
55_I95_F2.4961.00
109_E146_K2.4711.00
137_P189_E2.2531.00
138_L185_K2.1851.00
3_L124_A2.1491.00
193_D197_L2.1081.00
136_S139_G2.0551.00
74_S127_I1.9941.00
4_N50_E1.9711.00
50_E84_N1.9251.00
43_F132_Y1.9151.00
5_C51_C1.9131.00
201_L209_G1.9011.00
134_S191_L1.9011.00
74_S87_L1.9001.00
267_D274_Y1.9001.00
141_T188_R1.8731.00
129_G144_F1.8671.00
70_L95_F1.8511.00
201_L212_N1.8331.00
133_D188_R1.8301.00
126_I176_I1.8161.00
70_L119_V1.8081.00
3_L128_L1.8061.00
177_E181_N1.7961.00
5_C33_M1.7901.00
190_A236_F1.7901.00
164_K167_D1.7881.00
218_E258_R1.7761.00
112_F118_S1.7521.00
8_T26_Q1.7071.00
90_F231_Y1.6881.00
13_Q58_T1.6801.00
55_I70_L1.6781.00
221_Q225_R1.6741.00
221_Q265_K1.6641.00
57_I68_A1.6631.00
65_E98_K1.6461.00
11_A19_K1.6421.00
7_V29_L1.6311.00
198_G213_I1.5771.00
126_I175_L1.5671.00
189_E193_D1.5281.00
267_D270_N1.5231.00
24_T101_E1.5061.00
176_I180_L1.5041.00
32_F121_A1.4891.00
23_K26_Q1.4891.00
138_L189_E1.4581.00
126_I179_L1.4551.00
26_Q30_T1.4531.00
124_A128_L1.4491.00
144_F191_L1.4341.00
138_L141_T1.4291.00
197_L212_N1.4251.00
78_V85_T1.4251.00
151_V176_I1.4121.00
3_L33_M1.4071.00
67_K96_S1.3991.00
186_P189_E1.3971.00
244_I263_L1.3941.00
213_I247_I1.3631.00
187_F232_L1.3581.00
193_D219_L1.3471.00
270_N273_K1.3391.00
53_F70_L1.3361.00
260_Y264_S1.3311.00
51_C127_I1.3271.00
120_L124_A1.3231.00
33_M53_F1.3201.00
60_Y66_F1.3061.00
126_I147_A1.3011.00
2_E43_F1.3011.00
190_A216_A1.2901.00
218_E221_Q1.2891.00
199_L247_I1.2861.00
141_T145_Q1.2851.00
214_L259_V1.2831.00
236_F244_I1.2831.00
31_E35_N1.2821.00
149_N153_N1.2751.00
179_L232_L1.2711.00
244_I259_V1.2661.00
201_L204_E1.2621.00
25_L29_L1.2611.00
68_A95_F1.2561.00
129_G147_A1.2481.00
14_I57_I1.2471.00
187_F191_L1.2441.00
217_L262_I1.2441.00
189_E219_L1.2411.00
215_E218_E1.2321.00
175_L235_V1.2281.00
264_S275_E1.2271.00
145_Q148_Q1.2251.00
50_E75_S1.2131.00
212_N215_E1.2101.00
219_L222_K1.2091.00
20_Q101_E1.2081.00
64_N98_K1.1901.00
237_F263_L1.1881.00
193_D212_N1.1851.00
2_E39_T1.1821.00
132_Y143_Y1.1771.00
107_F121_A1.1771.00
72_V127_I1.1661.00
122_F126_I1.1651.00
262_I266_I1.1391.00
210_R255_E1.1251.00
65_E96_S1.1251.00
220_L233_L1.1241.00
58_T69_T1.1171.00
171_N174_W1.1171.00
206_P209_G1.1171.00
241_S270_N1.0991.00
270_N274_Y1.0951.00
230_S233_L1.0941.00
78_V199_L1.0941.00
29_L55_I1.0931.00
183_R227_R1.0911.00
241_S273_K1.0901.00
130_L144_F1.0881.00
129_G179_L1.0851.00
257_T261_E1.0771.00
22_F60_Y1.0751.00
242_D273_K1.0751.00
185_K189_E1.0741.00
3_L51_C1.0711.00
146_K150_I1.0701.00
3_L127_I1.0551.00
182_P185_K1.0531.00
210_R251_A1.0511.00
138_L188_R1.0461.00
39_T128_L1.0391.00
225_R265_K1.0381.00
197_L201_L1.0351.00
193_D215_E1.0341.00
128_L132_Y1.0271.00
192_Y196_R1.0231.00
75_S84_N1.0231.00
142_P145_Q1.0231.00
190_A220_L1.0231.00
7_V33_M1.0151.00
117_T121_A1.0151.00
118_S150_I1.0101.00
186_P222_K0.9870.99
78_V83_Y0.9860.99
24_T99_Y0.9750.99
173_Y177_E0.9690.99
246_N249_S0.9600.99
69_T91_N0.9550.99
126_I130_L0.9530.99
248_F259_V0.9500.99
130_L179_L0.9490.99
174_W178_N0.9410.99
28_A99_Y0.9320.99
107_F125_Y0.9320.99
201_L205_N0.9270.99
141_T180_L0.9260.99
88_L235_V0.9240.99
36_R106_E0.9230.99
132_Y136_S0.9050.99
100_V103_Q0.9050.99
218_E262_I0.8980.99
112_F154_A0.8890.99
25_L57_I0.8870.99
145_Q180_L0.8860.99
199_L202_M0.8860.99
252_P255_E0.8860.99
128_L143_Y0.8850.99
57_I66_F0.8800.99
211_A215_E0.8760.99
254_E258_R0.8720.99
122_F176_I0.8670.99
258_R261_E0.8670.99
39_T143_Y0.8550.99
113_D118_S0.8500.98
237_F241_S0.8490.98
209_G212_N0.8460.98
56_T69_T0.8430.98
203_A209_G0.8420.98
137_P193_D0.8420.98
133_D138_L0.8420.98
222_K226_R0.8380.98
144_F176_I0.8370.98
112_F157_S0.8360.98
170_R234_Q0.8350.98
115_N119_V0.8290.98
209_G213_I0.8230.98
201_L208_E0.8230.98
24_T102_F0.8190.98
146_K149_N0.8170.98
195_H240_K0.8170.98
122_F151_V0.8150.98
24_T28_A0.8140.98
69_T93_K0.8130.98
259_V262_I0.8100.98
80_N204_E0.8030.98
90_F238_D0.7990.98
212_N216_A0.7980.98
49_I127_I0.7880.98
125_Y150_I0.7870.98
61_P65_E0.7850.97
112_F115_N0.7840.97
38_W121_A0.7820.97
92_D172_R0.7810.97
241_S245_V0.7810.97
59_E62_S0.7790.97
245_V273_K0.7740.97
53_F72_V0.7720.97
2_E37_K0.7700.97
144_F179_L0.7700.97
20_Q23_K0.7700.97
251_A256_K0.7670.97
36_R107_F0.7640.97
94_D161_K0.7610.97
206_P210_R0.7550.97
97_F119_V0.7550.97
252_P256_K0.7540.97
62_S65_E0.7510.97
61_P67_K0.7480.97
222_K225_R0.7480.97
217_L259_V0.7450.97
133_D144_F0.7450.97
90_F130_L0.7440.97
87_L191_L0.7440.97
37_K42_V0.7400.96
5_C34_N0.7370.96
211_A255_E0.7370.96
130_L191_L0.7360.96
87_L130_L0.7330.96
240_K243_E0.7310.96
78_V135_F0.7270.96
108_N113_D0.7260.96
80_N203_A0.7210.96
255_E258_R0.7150.96
174_W177_E0.7120.96
49_I128_L0.7100.95
11_A22_F0.7050.95
191_L232_L0.7050.95
208_E212_N0.7050.95
231_Y235_V0.7040.95
25_L60_Y0.7010.95
234_Q238_D0.7010.95
176_I179_L0.7010.95
220_L266_I0.7000.95
64_N100_V0.6980.95
121_A150_I0.6960.95
6_T52_N0.6900.95
55_I120_L0.6890.95
184_Y230_S0.6890.95
183_R226_R0.6860.95
74_S131_D0.6860.95
121_A125_Y0.6840.94
259_V263_L0.6790.94
130_L232_L0.6790.94
21_V66_F0.6780.94
253_P257_T0.6770.94
271_S275_E0.6770.94
264_S271_S0.6750.94
9_V26_Q0.6740.94
202_M247_I0.6720.94
41_D143_Y0.6710.94
88_L123_Y0.6710.94
273_K276_K0.6710.94
221_Q262_I0.6670.94
6_T30_T0.6630.93
148_Q152_N0.6630.93
39_T43_F0.6600.93
85_T199_L0.6600.93
235_V239_A0.6570.93
147_A176_I0.6560.93
68_A97_F0.6550.93
11_A14_I0.6510.93
71_Q91_N0.6500.93
8_T54_L0.6480.93
245_V249_S0.6480.93
55_I68_A0.6460.93
139_G142_P0.6460.93
14_I17_T0.6430.92
190_A219_L0.6420.92
7_V55_I0.6410.92
119_V172_R0.6400.92
162_G167_D0.6390.92
224_N230_S0.6390.92
12_Q57_I0.6350.92
186_P219_L0.6340.92
27_T31_E0.6320.92
272_S275_E0.6320.92
269_S272_S0.6310.92
112_F159_E0.6310.92
213_I216_A0.6290.92
198_G216_A0.6290.92
257_T260_Y0.6280.92
208_E211_A0.6270.91
260_Y276_K0.6270.91
260_Y275_E0.6250.91
241_S267_D0.6240.91
55_I119_V0.6220.91
223_V233_L0.6210.91
7_V53_F0.6210.91
54_L71_Q0.6210.91
61_P96_S0.6190.91
224_N233_L0.6190.91
207_D211_A0.6180.91
184_Y223_V0.6170.91
109_E149_N0.6110.90
148_Q180_L0.6090.90
107_F146_K0.6050.90
245_V276_K0.6020.90
256_K260_Y0.6010.90
224_N266_I0.6000.90
68_A119_V0.5990.90
94_D168_G0.5940.89
125_Y146_K0.5880.89
248_F251_A0.5870.89
242_D246_N0.5860.88
21_V24_T0.5830.88
77_P135_F0.5820.88
57_I60_Y0.5780.88
187_F233_L0.5780.88
15_Q18_N0.5770.88
167_D171_N0.5750.88
187_F190_A0.5750.88
133_D139_G0.5740.87
144_F188_R0.5720.87
260_Y274_Y0.5720.87
107_F117_T0.5710.87
133_D141_T0.5700.87
19_K23_K0.5690.87
33_M51_C0.5660.87
106_E113_D0.5660.87
114_S117_T0.5620.86
20_Q24_T0.5620.86
137_P197_L0.5610.86
45_P48_R0.5610.86
126_I144_F0.5600.86
25_L55_I0.5600.86
147_A150_I0.5600.86
231_Y234_Q0.5590.86
28_A105_L0.5580.86
253_P256_K0.5570.86
180_L188_R0.5570.86
2_E128_L0.5560.86
187_F236_F0.5560.86
58_T67_K0.5550.86
207_D255_E0.5540.86
214_L258_R0.5540.86
86_P239_A0.5540.86
188_R223_V0.5530.85
14_I19_K0.5530.85
15_Q19_K0.5530.85
214_L251_A0.5530.85
53_F120_L0.5480.85
242_D245_V0.5450.85
109_E117_T0.5450.85
114_S118_S0.5440.85
187_F223_V0.5410.84
75_S86_P0.5390.84
237_F266_I0.5360.84
76_R200_D0.5360.84
150_I153_N0.5360.84
17_T21_V0.5360.84
16_G22_F0.5360.84
9_V55_I0.5340.83
260_Y271_S0.5330.83
30_T34_N0.5310.83
53_F127_I0.5310.83
16_G60_Y0.5300.83
96_S161_K0.5290.83
256_K259_V0.5270.83
251_A274_Y0.5270.83
40_N142_P0.5240.82
227_R231_Y0.5240.82
90_F175_L0.5230.82
29_L116_L0.5220.82
237_F274_Y0.5190.82
198_G212_N0.5170.81
6_T26_Q0.5150.81
33_M38_W0.5130.81
25_L97_F0.5130.81
25_L99_Y0.5130.81
224_N229_N0.5120.81
29_L33_M0.5120.81
186_P226_R0.5120.81
112_F153_N0.5110.81
118_S153_N0.5100.81
148_Q176_I0.5100.81
165_A174_W0.5100.81
88_L127_I0.5090.80
81_S203_A0.5090.80
33_M128_L0.5070.80
106_E114_S0.5070.80
202_M206_P0.5070.80
32_F120_L0.5070.80
61_P64_N0.5060.80
259_V274_Y0.5060.80
198_G201_L0.5050.80
92_D119_V0.5020.80
72_V91_N0.5000.79
162_G172_R0.5000.79
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2ouxA 2 0.2383 19.5 0.963 Contact Map
4bujB 1 0.2744 19 0.963 Contact Map
1g1eB 1 0.2419 12.7 0.966 Contact Map
1e91A 1 0.231 9.3 0.968 Contact Map
2katA 1 0.2996 8.8 0.968 Contact Map
4c0aA 1 0.2816 8.5 0.969 Contact Map
2zy9A 2 0.1697 7.6 0.969 Contact Map
4bmaA 1 0.1769 7 0.97 Contact Map
2f05A 1 0.231 6.8 0.97 Contact Map
3uswA 1 0.4043 6.6 0.97 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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