GREMLIN Database
DUF4831 - Domain of unknown function (DUF4831)
PFAM: PF16115 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 318 (313)
Sequences: 1634 (1384)
Seq/√Len: 78.2
META: 0.928

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
25_P45_D6.3301.00
146_I175_L4.9291.00
166_E169_K4.0701.00
174_E177_R3.8291.00
19_E52_K3.7061.00
213_F232_V3.4871.00
169_K173_D3.3651.00
56_I280_A3.2321.00
16_V213_F3.2141.00
214_R229_G3.2061.00
33_E39_K3.0271.00
23_Y42_I2.9861.00
19_E50_E2.8861.00
137_E141_K2.8371.00
20_K193_E2.7371.00
38_I41_V2.6031.00
66_Q263_R2.5281.00
18_A49_Y2.5181.00
24_K45_D2.5171.00
15_T196_T2.5121.00
12_L286_I2.4241.00
38_I187_T2.4121.00
13_R59_Y2.3001.00
20_K47_T2.2981.00
148_E152_D2.2621.00
117_N120_D2.2141.00
18_A197_Y2.2121.00
153_L171_M2.0801.00
130_K138_E2.0781.00
92_I260_L2.0721.00
23_Y190_T2.0701.00
70_V260_L2.0531.00
149_S152_D2.0391.00
12_L284_V1.9571.00
13_R200_T1.9491.00
17_E194_T1.9451.00
235_E271_T1.9261.00
278_T281_S1.8871.00
6_S67_V1.8731.00
9_K61_E1.8721.00
149_S155_T1.8441.00
268_A286_I1.8441.00
15_T198_T1.7951.00
147_R179_E1.7921.00
173_D176_D1.7911.00
261_Y292_V1.7731.00
68_Y292_V1.7601.00
151_N155_T1.7291.00
19_E192_T1.7231.00
178_Q182_Y1.7071.00
267_K283_R1.6771.00
51_I215_F1.6721.00
201_V234_I1.6501.00
11_V202_I1.6291.00
139_A185_L1.6121.00
152_D158_A1.6041.00
12_L58_T1.5991.00
13_R57_T1.5951.00
176_D180_K1.5931.00
13_R198_T1.5831.00
49_Y195_Q1.5720.99
17_E53_S1.5700.99
15_T55_K1.5630.99
18_A221_I1.5320.99
21_T192_T1.5210.99
154_L171_M1.5180.99
234_I272_I1.5100.99
150_R176_D1.5080.99
56_I270_V1.4980.99
89_L310_F1.4960.99
91_A94_A1.4900.99
16_V54_I1.4830.99
134_K137_E1.4820.99
168_L171_M1.4800.99
236_L270_V1.4730.99
22_T193_E1.4630.99
15_T57_T1.4580.99
153_L167_A1.4560.99
219_L228_S1.4520.99
142_Q145_E1.4400.99
5_Y8_P1.4380.99
84_T90_L1.4350.99
14_V272_I1.4340.99
2_G69_V1.4300.99
60_G313_E1.4210.99
210_E234_I1.4120.99
27_P37_G1.4110.99
212_L234_I1.3900.99
23_Y46_S1.3860.99
230_E274_D1.3830.99
201_V210_E1.3700.99
311_D314_T1.3620.99
158_A162_P1.3580.99
3_V81_L1.3570.98
214_R228_S1.3530.98
212_L272_I1.3510.98
17_E52_K1.3380.98
214_R231_P1.3260.98
10_T287_A1.3110.98
17_E196_T1.2890.98
12_L56_I1.2830.98
82_S91_A1.2810.98
238_P241_T1.2760.98
14_V212_L1.2720.98
150_R172_L1.2650.98
9_K64_P1.2650.98
222_V228_S1.2590.98
114_K120_D1.2510.97
237_K269_N1.2410.97
261_Y294_S1.2410.97
179_E183_L1.2390.97
237_K271_T1.2360.97
116_L120_D1.2270.97
20_K49_Y1.2260.97
211_V231_P1.1920.97
214_R226_D1.1850.96
31_Y133_A1.1750.96
269_N281_S1.1680.96
270_V284_V1.1660.96
16_V51_I1.1620.96
151_N175_L1.1600.96
162_P168_L1.1470.96
230_E275_G1.1360.95
308_V317_I1.1320.95
59_Y200_T1.1240.95
214_R227_L1.1210.95
14_V56_I1.1100.95
116_L121_Y1.1070.95
115_T120_D1.0930.94
114_K121_Y1.0830.94
126_I139_A1.0820.94
215_F232_V1.0820.94
117_N121_Y1.0800.94
167_A171_M1.0780.94
267_K285_P1.0700.94
139_A142_Q1.0610.93
142_Q155_T1.0550.93
236_L268_A1.0530.93
21_T48_S1.0510.93
263_R292_V1.0510.93
184_S189_K1.0410.93
173_D177_R1.0410.93
25_P30_K1.0330.93
11_V200_T1.0290.92
122_L125_E1.0200.92
4_V69_V1.0120.92
158_A167_A1.0110.92
52_K194_T1.0040.91
121_Y126_I0.9900.91
118_P121_Y0.9750.90
12_L280_A0.9680.90
207_V210_E0.9640.90
270_V286_I0.9630.90
54_I272_I0.9570.89
103_P108_P0.9450.89
22_T47_T0.9440.89
126_I138_E0.9380.88
16_V199_F0.9360.88
209_K235_E0.9310.88
142_Q182_Y0.9240.88
34_K133_A0.9190.87
184_S187_T0.9190.87
172_L176_D0.9170.87
254_K258_G0.9010.86
153_L168_L0.8990.86
28_Y36_L0.8980.86
225_D229_G0.8940.86
54_I213_F0.8930.86
119_R122_L0.8920.86
163_P166_E0.8880.86
150_R179_E0.8820.85
170_L174_E0.8740.85
84_T88_L0.8730.85
266_G287_A0.8720.84
255_K258_G0.8680.84
215_F222_V0.8610.84
6_S62_P0.8530.83
124_E141_K0.8510.83
264_I293_E0.8500.83
34_K183_L0.8500.83
120_D123_T0.8500.83
216_S220_G0.8450.83
226_D231_P0.8440.82
11_V61_E0.8440.82
222_V229_G0.8380.82
118_P123_T0.8370.82
60_G287_A0.8340.82
271_T281_S0.8330.82
61_E283_R0.8320.82
177_R181_A0.8300.81
19_E194_T0.8300.81
152_D157_E0.8290.81
105_P108_P0.8290.81
142_Q151_N0.8260.81
5_Y261_Y0.8250.81
61_E64_P0.8190.81
76_T80_F0.8180.80
149_S158_A0.8150.80
144_Y149_S0.8140.80
73_K302_K0.8140.80
201_V236_L0.8100.80
311_D317_I0.8090.80
88_L311_D0.8080.80
66_Q292_V0.8060.80
146_I233_Y0.8040.79
262_Y310_F0.8040.79
307_K317_I0.8020.79
106_F109_A0.7990.79
209_K233_Y0.7960.79
2_G7_L0.7940.78
252_K257_K0.7920.78
241_T266_G0.7910.78
49_Y215_F0.7890.78
14_V201_V0.7890.78
9_K202_I0.7790.77
5_Y10_T0.7780.77
158_A163_P0.7770.77
209_K273_S0.7760.77
11_V59_Y0.7730.77
26_G186_F0.7730.77
153_L160_N0.7620.76
164_D169_K0.7600.75
215_F220_G0.7590.75
157_E168_L0.7580.75
114_K119_R0.7580.75
242_T245_L0.7570.75
35_Y133_A0.7500.74
269_N283_R0.7490.74
119_R126_I0.7490.74
178_Q181_A0.7490.74
310_F315_G0.7470.74
29_A32_A0.7410.74
9_K204_D0.7410.74
156_G168_L0.7380.73
18_A199_F0.7380.73
197_Y221_I0.7370.73
287_A314_T0.7370.73
113_L117_N0.7370.73
180_K184_S0.7370.73
168_L172_L0.7370.73
14_V199_F0.7350.73
232_V272_I0.7350.73
166_E174_E0.7340.73
238_P242_T0.7340.73
145_E149_S0.7320.73
147_R165_G0.7290.72
152_D155_T0.7290.72
211_V224_A0.7290.72
114_K117_N0.7280.72
23_Y45_D0.7280.72
158_A172_L0.7280.72
76_T79_P0.7280.72
27_P138_E0.7280.72
251_E254_K0.7240.72
120_D124_E0.7160.71
234_I270_V0.7150.71
183_L187_T0.7120.71
138_E182_Y0.7100.70
127_L144_Y0.7100.70
175_L230_E0.7040.70
296_P306_T0.7000.69
158_A171_M0.6980.69
33_E38_I0.6960.69
32_A236_L0.6950.69
3_V70_V0.6950.69
114_K118_P0.6940.69
29_A43_T0.6940.69
154_L172_L0.6930.68
58_T284_V0.6910.68
25_P29_A0.6890.68
21_T50_E0.6890.68
142_Q150_R0.6880.68
166_E173_D0.6870.68
156_G162_P0.6870.68
305_P316_A0.6860.68
93_N134_K0.6840.67
131_S229_G0.6840.67
259_G295_L0.6820.67
84_T87_G0.6820.67
103_P107_K0.6810.67
254_K257_K0.6810.67
116_L122_L0.6800.67
214_R223_D0.6780.67
211_V214_R0.6750.66
214_R225_D0.6750.66
10_T286_I0.6750.66
180_K187_T0.6740.66
122_L126_I0.6730.66
309_E317_I0.6720.66
271_T278_T0.6720.66
42_I45_D0.6720.66
115_T121_Y0.6680.66
171_M197_Y0.6660.65
6_S143_I0.6660.65
237_K286_I0.6650.65
211_V233_Y0.6640.65
36_L185_L0.6620.65
24_K191_T0.6620.65
272_I279_L0.6610.65
36_L183_L0.6610.65
210_E224_A0.6600.65
146_I179_E0.6590.65
287_A315_G0.6590.65
78_A83_L0.6580.64
106_F111_K0.6580.64
296_P305_P0.6570.64
131_S231_P0.6570.64
135_K148_E0.6560.64
264_I291_T0.6540.64
206_E240_E0.6520.64
49_Y162_P0.6490.63
121_Y124_E0.6490.63
72_F83_L0.6460.63
20_K195_Q0.6440.63
156_G161_M0.6440.63
60_G88_L0.6430.63
222_V271_T0.6420.63
12_L270_V0.6410.62
115_T119_R0.6410.62
16_V159_D0.6390.62
28_Y32_A0.6370.62
273_S278_T0.6370.62
121_Y125_E0.6370.62
6_S64_P0.6370.62
115_T118_P0.6360.62
109_A270_V0.6350.62
135_K144_Y0.6350.62
251_E258_G0.6340.62
172_L278_T0.6330.61
119_R123_T0.6320.61
129_A147_R0.6320.61
129_A162_P0.6310.61
86_D215_F0.6260.61
222_V227_L0.6240.60
27_P151_N0.6230.60
15_T159_D0.6180.60
123_T126_I0.6160.59
218_K228_S0.6140.59
36_L139_A0.6120.59
230_E235_E0.6120.59
130_K263_R0.6120.59
89_L294_S0.6100.59
269_N282_G0.6090.59
86_D267_K0.6080.58
79_P89_L0.6080.58
146_I150_R0.6070.58
239_L244_A0.6070.58
139_A152_D0.6070.58
82_S96_P0.6060.58
235_E273_S0.6050.58
273_S276_N0.6050.58
94_A309_E0.6040.58
248_K254_K0.6040.58
164_D167_A0.6020.58
116_L119_R0.6020.58
102_E105_P0.6010.58
131_S134_K0.6000.57
165_G186_F0.5990.57
260_L263_R0.5970.57
198_T202_I0.5960.57
131_S140_A0.5950.57
129_A225_D0.5940.57
221_I317_I0.5900.56
100_E112_S0.5870.56
211_V226_D0.5870.56
181_A189_K0.5870.56
307_K310_F0.5860.56
232_V274_D0.5850.56
239_L242_T0.5820.55
100_E103_P0.5800.55
201_V207_V0.5800.55
132_T135_K0.5790.55
250_A255_K0.5780.55
308_V313_E0.5770.55
211_V222_V0.5770.55
117_N125_E0.5770.55
38_I144_Y0.5730.54
214_R222_V0.5730.54
286_I313_E0.5730.54
2_G172_L0.5730.54
153_L158_A0.5720.54
294_S297_P0.5690.53
58_T313_E0.5690.53
9_K51_I0.5690.53
46_S191_T0.5680.53
264_I308_V0.5650.53
199_F212_L0.5640.53
145_E152_D0.5640.53
17_E55_K0.5620.53
98_P101_E0.5600.52
62_P88_L0.5600.52
156_G229_G0.5580.52
103_P106_F0.5580.52
305_P308_V0.5580.52
102_E106_F0.5570.52
118_P124_E0.5560.52
246_V250_A0.5560.52
146_I151_N0.5550.52
199_F221_I0.5550.52
181_A184_S0.5550.52
216_S289_F0.5540.52
134_K139_A0.5540.52
120_D126_I0.5520.51
154_L183_L0.5500.51
302_K309_E0.5480.51
131_S214_R0.5460.51
153_L156_G0.5450.50
214_R224_A0.5450.50
103_P109_A0.5440.50
204_D278_T0.5440.50
232_V262_Y0.5430.50
157_E160_N0.5410.50
129_A138_E0.5400.50
233_Y273_S0.5400.50
36_L41_V0.5390.50
131_S227_L0.5390.50
250_A256_K0.5390.50
236_L286_I0.5380.50
292_V296_P0.5380.50
75_K230_E0.5380.50
187_T200_T0.5360.49
158_A168_L0.5360.49
312_P315_G0.5340.49
14_V234_I0.5340.49
219_L226_D0.5310.49
239_L269_N0.5310.49
36_L143_I0.5300.48
313_E316_A0.5300.48
132_T150_R0.5290.48
73_K178_Q0.5280.48
52_K196_T0.5270.48
224_A272_I0.5260.48
136_A140_A0.5260.48
154_L160_N0.5250.48
30_K137_E0.5240.48
295_L300_F0.5240.48
27_P182_Y0.5240.48
85_E157_E0.5220.47
128_V143_I0.5220.47
180_K200_T0.5210.47
97_E100_E0.5190.47
312_P316_A0.5180.47
310_F317_I0.5170.47
109_A112_S0.5170.47
139_A155_T0.5160.47
67_V89_L0.5160.47
182_Y185_L0.5150.47
61_E101_E0.5140.46
163_P168_L0.5130.46
75_K80_F0.5130.46
260_L310_F0.5110.46
51_I199_F0.5110.46
137_E227_L0.5110.46
234_I273_S0.5110.46
31_Y136_A0.5100.46
149_S163_P0.5090.46
207_V234_I0.5070.46
248_K257_K0.5070.46
51_I274_D0.5060.45
260_L265_P0.5050.45
151_N157_E0.5050.45
293_E296_P0.5030.45
18_A195_Q0.5030.45
114_K126_I0.5030.45
216_S231_P0.5020.45
141_K148_E0.5020.45
148_E155_T0.5010.45
240_E266_G0.5010.45
131_S162_P0.5000.45
240_E244_A0.5000.45
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1r48A 2 0.1038 12.1 0.968 Contact Map
1cbyA 2 0.1918 11.2 0.968 Contact Map
3ronA 1 0.1698 10.7 0.968 Contact Map
2bzbA 2 0.195 6.9 0.971 Contact Map
3mceA 2 0.1761 5.9 0.972 Contact Map
2qmeI 1 0.0755 5.6 0.972 Contact Map
4hw6A 3 0.3491 5.5 0.972 Contact Map
2c0sA 1 0.2013 5.2 0.973 Contact Map
2ov2I 1 0.0755 4.9 0.973 Contact Map
1f3mA 2 0.0629 4.7 0.973 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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