GREMLIN Database
Tox-GHH - GHH signature containing HNH/Endo VII superfamily nuclease toxin
PFAM: PF15636 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 78 (72)
Sequences: 741 (579)
Seq/√Len: 68.2
META: 0.741

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
44_I50_V3.3211.00
60_S63_Q3.0731.00
40_E53_Y2.9361.00
15_V55_G2.7661.00
26_V44_I2.6211.00
23_R27_R2.5391.00
7_L61_V2.3321.00
42_Q46_S2.2761.00
13_R17_R2.2461.00
14_A69_G2.2311.00
25_L30_G1.9791.00
41_K45_L1.9311.00
18_A76_F1.9031.00
13_R69_G1.8501.00
17_R20_K1.7861.00
27_R45_L1.7231.00
43_E51_P1.5720.99
56_H59_N1.5650.99
24_E28_E1.4430.98
47_T50_V1.4380.98
14_A17_R1.4050.98
25_L33_T1.3770.98
29_G41_K1.2840.97
25_L29_G1.2110.95
10_A13_R1.1190.93
20_K62_E1.1040.92
10_A69_G1.0100.89
24_E37_T1.0080.88
19_W53_Y0.9820.87
47_T51_P0.9570.86
23_R28_E0.9410.85
11_R15_V0.9280.84
11_R61_V0.9260.84
67_L70_D0.9120.83
19_W50_V0.9040.82
54_E57_H0.8970.82
21_Q72_N0.8670.80
11_R33_T0.8620.79
21_Q24_E0.8230.76
73_N76_F0.8210.76
26_V29_G0.8170.75
58_I73_N0.8060.74
24_E71_P0.7960.74
55_G74_I0.7960.74
26_V32_G0.7940.73
11_R57_H0.7930.73
21_Q42_Q0.7920.73
39_E46_S0.7860.73
59_N64_Y0.7760.72
6_L10_A0.7720.71
23_R45_L0.7630.70
60_S64_Y0.7620.70
43_E52_G0.7540.69
23_R40_E0.7540.69
43_E50_V0.7480.69
44_I53_Y0.7320.67
32_G36_W0.7280.67
39_E43_E0.7070.64
22_E36_W0.7000.64
13_R16_R0.6990.64
9_H13_R0.6960.63
37_T40_E0.6900.63
57_H60_S0.6890.62
8_E12_Q0.6880.62
50_V57_H0.6830.62
59_N67_L0.6760.61
10_A14_A0.6750.61
43_E47_T0.6730.61
19_W76_F0.6720.61
38_E46_S0.6700.60
67_L72_N0.6670.60
26_V36_W0.6620.59
39_E47_T0.6590.59
17_R71_P0.6570.59
15_V18_A0.6500.58
7_L68_A0.6370.56
61_V68_A0.6350.56
17_R47_T0.6350.56
14_A18_A0.6230.55
34_R75_Q0.6220.55
43_E49_K0.6210.55
36_W50_V0.6200.54
46_S49_K0.6200.54
18_A74_I0.6130.54
21_Q71_P0.6060.53
43_E48_G0.6030.52
34_R53_Y0.6020.52
16_R46_S0.5990.52
38_E41_K0.5880.51
11_R14_A0.5830.50
8_E17_R0.5820.50
65_P68_A0.5720.49
8_E66_E0.5630.48
40_E50_V0.5500.46
29_G32_G0.5490.46
51_P75_Q0.5450.46
65_P69_G0.5400.45
43_E65_P0.5300.44
7_L10_A0.5300.44
49_K76_F0.5230.43
32_G73_N0.5180.42
44_I52_G0.5100.41
27_R38_E0.5090.41
14_A55_G0.5090.41
6_L76_F0.5050.41
33_T58_I0.5030.41
25_L60_S0.5000.40
12_Q39_E0.5000.40
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3mabA 2 0.6923 7.5 0.909 Contact Map
2xsaA 2 0.7051 6.2 0.913 Contact Map
4pysA 1 1 4.7 0.918 Contact Map
3dmyA 2 0.2821 4.3 0.919 Contact Map
7ceiB 1 0.8846 4.2 0.919 Contact Map
4jg2A 1 0.1282 4.2 0.919 Contact Map
4ogeA 1 0.8974 4.1 0.92 Contact Map
3hwkA 5 0.641 4.1 0.92 Contact Map
1usuB 1 0.0256 4.1 0.92 Contact Map
3zfkA 1 0.8846 3.8 0.921 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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